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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉麗飛(Li- Fei Liu) | |
| dc.contributor.author | Ya-Fin Lin | en |
| dc.contributor.author | 林雅芬 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:51:52Z | - |
| dc.date.available | 2007-07-30 | |
| dc.date.copyright | 2005-07-30 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-28 | |
| dc.identifier.citation | 曾東海、鄭清煥、陳治官、顏信沐、鄭統隆、卓緯玄和王強生 (2003) 水稻臺農67號同源突變系對褐飛蝨之抗性探討。中華農藝學會九十二年會作物科學講座暨研究成果發表會。中華農藝學會。第30頁。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35416 | - |
| dc.description.abstract | 水稻穎花大小與形態可能限制榖粒發育，間接影響產量；因此，本試驗期望找到與水稻穎花發育相關的基因。從劉(2003)所建立水稻穎花發育相關候選基因庫中，挑選6個基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型穎花(SLP/SLP)、小穎花突變體(SLP/slp)及內外穎退化突變體(slp/slp)之葉片及1~4公分幼花序為材料，利用Real-Time RT-PCR分析，發現salT基因在突變體幼花序中大量表現。因此，進一步分析salT基因在水稻不同器官中的表現，發現salT基因在水稻中僅有一個拷貝數，葉鞘salT基因表現量比葉身高，在SLP/SLP幼花序中幾乎不表現，但在slp/slp幼花序中表現量極高。salT基因在水稻中僅有一個拷貝數；本試驗進一步利用農桿菌轉殖法將salT基因轉殖到TNG67及SLP/SLP品系中，分析salT基因對轉殖株穎花形態之影響。在外表性狀上，持續性表現salT基因之TNG67轉殖株，會使株高變矮，且均不結實，類似slp/slp形態；然而，在促進或抑制salT基因表現之SLP/SLP轉殖系中，植株株高及穎花形態均與非轉殖株無太大差異；因此，水稻穎花構造之變異與salT基因的關係，尚須進一步確認。在非生物逆境研究方面，誘導表現salT基因可能與水稻3-4葉齡幼苗的耐旱性有關，在耐鹽性與耐冷性方面未見特殊影響，須再經試驗確認。 | zh_TW |
| dc.description.abstract | The size and shape of lemma and palea may limit the developing of rice grains, that could affect the rice yield indirectly. Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea (SLP/SLP), smaller lemma/palea (SLP/slp), and stunted lemma/palea (slp/slp) was compared by Real Time RT-PCR. The results showed that salT expression is higher in leaf sheath than in leaf lamina, and is especially high in the inflorescence of slp/slp mutant. The Southern blot showed that only one copy of salT gene in rice genome. SalT gene was transferred to TNG67 and SLP/SLP rice plant by Agro-transformation method. Three kinds of constructs were designed for over expression, induced expression, and knock out expression of salT gene. The transgenic rice plants with over expressed of salT gene in TNG67 were lower and no spikelets. However, the plant height and the size of lemma/palea of SLP/SLP transgenic rice plants showed no difference to the non-transgenic rice plants. Therefore, the relationship between salT and the structure of spikelets must be studied in more details. The transgenic rice plants with over expressed salT gene showed higher drought tolerance, but no special performance under salt or cold stress. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:51:52Z (GMT). No. of bitstreams: 1 ntu-94-R92621111-1.pdf: 2005638 bytes, checksum: ad5a10782e95e162760b619d43c980d3 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄-------------------------------------------------------------------------------------------------i
圖表目錄--------------------------------------------------------------------------------------ii 附錄目錄-------------------------------------------------------------------------------------iii 縮寫對照表--------------------------------------------------------------------------------------iv 中文摘要------------------------------------------------------------------------------------------v 英文摘要-----------------------------------------------------------------------------------------vi 壹、前言------------------------------------------------------------------------------------------1 貳、文獻回顧 Ⅰ.水稻穎花發育之研究-----------------------------------------------------------------3 Ⅱ.SLP/slp小穎花突變體之研究------------------------------------------------------7 Ⅲ.水稻穎花發育候選基因之探討-----------------------------------------------------9 參、實驗設計與流程--------------------------------------------------------------------------15 肆、材料與方法 Ⅰ.試驗材料-------------------------------------------------------------------------------16 Ⅱ.水稻穎花相關基因表現分析-------------------------------------------------------17 Ⅲ. 水稻內生salT基因拷貝數--------------------------------------------------------19 Ⅳ. 基因轉殖質體製備------------------------------------------------------------------24 Ⅴ. 水稻基因轉殖------------------------------------------------------------------------33 Ⅵ. 轉殖水稻T1代耐逆境測試--------------------------------------------------------41 伍、結果 Ⅰ. 水稻穎花相關基因表現分析------------------------------------------------------44 Ⅱ. 水稻內生salT基因表現特性分析-----------------------------------------------45 Ⅲ. salT基因轉殖水稻之分析---------------------------------------------------------47 Ⅳ. salT轉殖水稻農藝性狀調查------------------------------------------------------49 Ⅴ. salT轉殖水稻T1代分離率調查--------------------------------------------------50 Ⅵ. salT轉殖水稻對非生物性逆境之反應------------------------------------------50 陸、討論-----------------------------------------------------------------------------------------52 參考文獻-----------------------------------------------------------------------------------------59 圖表-----------------------------------------------------------------------------------------------70 附錄----------------------------------------------------------------------------------------------114 | |
| dc.language.iso | zh-TW | |
| dc.subject | salT基因 | zh_TW |
| dc.subject | 水稻轉殖 | zh_TW |
| dc.subject | 水稻穎花 | zh_TW |
| dc.subject | rice inflorescence | en |
| dc.subject | salT gene | en |
| dc.subject | transgenic rice | en |
| dc.title | 水稻穎花相關基因之研究 | zh_TW |
| dc.title | Studies on the Genes Related to Rice Spikelets | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張孟基(Men-Chi Chang),黃鵬林(Pung-Ling Huang),王強生(Chang-Sheng Wang) | |
| dc.subject.keyword | 水稻穎花,水稻轉殖,salT基因, | zh_TW |
| dc.subject.keyword | rice inflorescence,transgenic rice,salT gene, | en |
| dc.relation.page | 118 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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