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標題: | 利用鼷鼠淋巴癌細胞tk分析法檢測蛋黃油與紅麴米丙酮萃出物之基因毒性 Genotoxicity analysis of some egg yolk oils and acetone extract of Monascus-fermented rice by in vitro mouse lymphoma tk assay |
作者: | Yi-Chu Liu 劉怡初 |
指導教授: | 劉俊民 |
共同指導教授: | 蘇南維 |
關鍵字: | 基因毒性,鼷鼠淋巴癌細胞tk分析法,蛋黃油,紅麴米, Genotoxicity,mouse lymphoma tk assay,egg yolk oils,Monascus-fermented rice, |
出版年 : | 2005 |
學位: | 碩士 |
摘要: | 本研究利用鼷鼠淋巴癌細胞株 L5178Y (tk+/-) 法評估市售蛋黃油、自製蛋黃油及其經長時間加熱(260℃,48hr)所製的加工品與紅麴米丙酮萃出物之安全性。蛋油試驗品以絕對酒精溶解,紅麴米丙酮萃出物及其矽膠管柱區分物以 DMSO 溶解後,添加於含 5%馬血清之 RPMI1640 培養基 (內含 1 × 107 cells),均勻混和後,經過三小時的處理,進行細胞毒性試驗。結果發現,當三種蛋油樣品濃度為 1 mg/mL 時,細胞的致死率皆為 100%;三種蛋油樣品中以黑燒卵油(PYO)對試驗細胞株之細胞毒性最為顯著,濃度為 0.625 mg/mL 時其細胞致死率約為 30%;在紅麴米丙酮萃出物中,矽膠管柱區分物之乙酸乙酯區分物在濃度為0.055 mg/mL,細胞致死率為 40%。在基因毒性的測試方面,先將細胞以樣品處理三小時,之後以不含樣品之培養基培養二天後,再以含有嘧啶類似物 TFT (5-trifluorothymidine) 2 μg/mL的培養基培養存活下來之細胞,培養十二天之後,以計算其處理過後所誘發的突變率。結果顯示,三種蛋黃油試驗樣品及紅麴米丙酮萃出物及區分物不論是否經 S9 處理,皆未顯示有明顯的基因毒性。 In this study, mouse lymphoma cell L5178Y (tk+/-) assay was employed to detect the genotoxicity of the egg oil samples and the acetone extract of Monascus-fermented red rice. Three kinds of egg yolk oil samples and the acetone extract of Monascus-fermented rice and its silica gel fractions were dissolved in absolute ethanol and DMSO, respectively. In cytotoxicity tests, each sample with various concentrations was added into RPMI1640 medium with 5% horse serum containing 1 × 107 L5178Y tk+/- cells to incubate for three hours. Among the three kinds of egg oil samples, the PYO revealed the most cytotoxicity. When cells were incubated with 1 mg/mL of all three egg yolk oil samples, there were no cells survivals. When cells were incubated with 0.625 mg/mL of PYO, thirty percent of cells were dead. In acetone extract of Monascus-fermented rice, the ethyl acetate fraction of silica gel fractionation could cause 40% cell death at 0.055 mg/mL. In genotoxicity tests, a thymidine analogue TFT (5-trifluorothymidine) was applied to detect the mutation by 12-days incubation after the 2 days expression period followed the treatment. As the results, whether the three kinds of egg oil samples were treated with the S9 mix or not, no significant genotoxicity was shown. Similar non-genotoxic results were also observed in the acetone extract of Monascus-fermented red rice and its silica gel fractions. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35163 |
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顯示於系所單位: | 微生物學科所 |
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