請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34800完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王錦堂 | |
| dc.contributor.author | Yi-Ping Chuang | en |
| dc.contributor.author | 莊依萍 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:34:51Z | - |
| dc.date.available | 2006-02-08 | |
| dc.date.copyright | 2006-02-08 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2006-01-18 | |
| dc.identifier.citation | Abbott, S. (2003) Klebsiella, Enterobacter, Citrobacter, Serratia, Plesiomonas, and Other Enterobacteriaceae. In Manual of Clinical Microbiology. P.R. Murray, E.J.B., J.H. Jorgensen, M.A. Pfaller, and R.H. Yolken (ed). Washington D.C.: American Society for Microbiology Press, pp. 684-700.
Aguilar, J., Cruz, A., and Ortega, C. (1994) [Multiple hepatic and pulmonary abscesses caused by Klebsiella pneumoniae]. Enferm Infecc Microbiol Clin 12: 270-271. Alberti, S., Alvarez, D., Merino, S., Casado, M.T., Vivanco, F., Tomas, J.M., and Benedi, V.J. (1996) Analysis of complement C3 deposition and degradation on Klebsiella pneumoniae. Infect Immun 64: 4726-4732. Alvarez, D., Merino, S., Tomas, J.M., Benedi, V.J., and Alberti, S. (2000) Capsular polysaccharide is a major complement resistance factor in lipopolysaccharide O side chain-deficient Klebsiella pneumoniae clinical isolates. Infect Immun 68: 953-955. Amor, P.A., and Whitfield, C. (1997) Molecular and functional analysis of genes required for expression of group IB K antigens in Escherichia coli: characterization of the his-region containing gene clusters for multiple cell-surface polysaccharides. Mol Microbiol 26: 145-161. Arakawa, Y., Wacharotayankun, R., Nagatsuka, T., Ito, H., Kato, N., and Ohta, M. (1995) Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J Bacteriol 177: 1788-1796. Athamna, A., Ofek, I., Keisari, Y., Markowitz, S., Dutton, G.G., and Sharon, N. (1991) Lectinophagocytosis of encapsulated Klebsiella pneumoniae mediated by surface lectins of guinea pig alveolar macrophages and human monocyte-derived macrophages. Infect Immun 59: 1673-1682. Ayinala, S.R., Vulpe, M., Azaz, M., Cohen, H., Donelson, S.S., and Lee, M. (2001) Pyogenic liver abscesses due to Klebsiella pneumoniae in a diabetic patient. J Miss State Med Assoc 42: 67-70. Barton, E.N., Daisley, H., Gilbert, D.T., and Roberts, L. (1991) Diabetes mellitus and Klebsiella pneumoniae liver abscess in adults. Trop Geogr Med 43: 100-104. Belaaouaj, A., McCarthy, R., Baumann, M., Gao, Z., Ley, T.J., Abraham, S.N., and Shapiro, S.D. (1998) Mice lacking neutrophil elastase reveal impaired host defense against gram negative bacterial sepsis. Nat Med 4: 615-618. Bronner, D., Clarke, B.R., and Whitfield, C. (1994) Identification of an ATP-binding cassette transport system required for translocation of lipopolysaccharide O-antigen side-chains across the cytoplasmic membrane of Klebsiella pneumoniae serotype O1. Mol Microbiol 14: 505-519. Cahill, M., Chang, B., and Murray, A. (2000) Bilateral endogenous bacterial endophthalmitis associated with pyogenic hepatic abscess. Br J Ophthalmol 84: 1436. Casanova, C., Lorente, J.A., Carrillo, F., Perez-Rodriguez, E., and Nunez, N. (1989) Klebsiella pneumoniae liver abscess associated with septic endophthalmitis. Arch Intern Med 149: 1467. Chang, F.Y., and Chou, M.Y. (1995) Comparison of pyogenic liver abscesses caused by Klebsiella pneumoniae and non-K. pneumoniae pathogens. J Formos Med Assoc 94: 232-237. Chang, K.C., Yeh, Y.C., Lin, T.L., and Wang, J.T. (2001) Identification of genes associated with natural competence in Helicobacter pylori by transposon shuttle random mutagenesis. Biochem Biophys Res Commun 288: 961-968. Chang, S.C., Chang, H.J., and Lai, M.S. (1999) Antibiotic usage in primary care units in Taiwan. Int J Antimicrob Agents 11: 23-30. Chang, S.C., Fang, C.T., Hsueh, P.R., Chen, Y.C., and Luh, K.T. (2000) Klebsiella pneumoniae isolates causing liver abscess in Taiwan. Diagn Microbiol Infect Dis 37: 279-284. Cheng, D.L., Liu, Y.C., Yen, M.Y., Liu, C.Y., Shi, F.W., and Wang, L.S. (1990) Pyogenic liver abscess: clinical manifestations and value of percutaneous catheter drainage treatment. J Formos Med Assoc 89: 571-576. Cheng, D.L., Liu, Y.C., Yen, M.Y., Liu, C.Y., and Wang, R.S. (1991) Septic metastatic lesions of pyogenic liver abscess. Their association with Klebsiella pneumoniae bacteremia in diabetic patients. Arch Intern Med 151: 1557-1559. Chiu, C.T., Lin, D.Y., and Liaw, Y.F. (1988) Metastatic septic endophthalmitis in pyogenic liver abscess. J Clin Gastroenterol 10: 524-527. Chou, F.F., and Kou, H.K. (1996) Endogenous endophthalmitis associated with pyogenic hepatic abscess. J Am Coll Surg 182: 33-36. Chou, H.C., Lee, C.Z., Ma, L.C., Fang, C.T., Chang, S.C., and Wang, J.T. (2004) Isolation of a chromosomal region of Klebsiella pneumoniae associated with allantoin metabolism and liver infection. Infect Immun 72: 3783-3792. Cobo Martinez, F., Aliaga Martinez, L., Diaz Monllor, F., Mediavilla Garcia, J.D., Arrebola Nacle, J.P., and de la Rosa Fraile, M. (1999) [Liver abscess caused by Klebsiella pneumoniae in diabetic patients]. Rev Clin Esp 199: 517-519. Corredoira, J., Casariego, E., Moreno, C., Villanueva, L., Lopez, Varela, J., Rodriguez, A., Alonso, P., and Coira, A. (1998) Prospective study of Streptococcus milleri hepatic abscess. Eur J Clin Microbiol Infect Dis 17: 556-560. Couez, D., Libon, E., Wasteels, M., Derue, G., and Gilbeau, J.P. (1991) Klebsiella pneumoniae liver abscess with septic endophthalmitis. The role of computed tomography. J Belge Radiol 74: 41-44. Cryz, S.J., Jr., Furer, E., and Germanier, R. (1984) Protection against fatal Klebsiella pneumoniae burn wound sepsis by passive transfer of anticapsular polysaccharide. Infect Immun 45: 139-142. Cryz, S.J., Jr., Furer, E., and Germanier, R. (1985) Purification and vaccine potential of Klebsiella capsular polysaccharides. Infect Immun 50: 225-230. Darfeuille-Michaud, A., Jallat, C., Aubel, D., Sirot, D., Rich, C., Sirot, J., and Joly, B. (1992) R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections. Infect Immun 60: 44-55. de Lorenzo, V., Herrero, M., Jakubzik, U., and Timmis, K.N. (1990) Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172: 6568-6572. Eisenstein, B.I., and D.F. Zaleznik. (2000) Enterobacteriaceae. In Principles and Practice of Infectious Diseases. Mandell, J.E.B., and R. Dolin, (ed). Philadelphia: Churchill-Livingstone, pp. 2294-2309. Fang, C.T., Chang, S.C., Hsueh, P.R., Chen, Y.C., Sau, W.Y., and Luh, K.T. (2000a) Microbiologic features of adult community-acquired bacterial meningitis in Taiwan. J Formos Med Assoc 99: 300-304. Fang, C.T., Chen, Y.C., Chang, S.C., Sau, W.Y., and Luh, K.T. (2000b) Klebsiella pneumoniae meningitis: timing of antimicrobial therapy and prognosis. Qjm 93: 45-53. Fang, C.T., Chuang, Y.P., Shun, C.T., Chang, S.C., and Wang, J.T. (2004) A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications. J Exp Med 199: 697-705. Fung, C.P., Hu, B.S., Chang, F.Y., Lee, S.C., Kuo, B.I., Ho, M., Siu, L.K., and Liu, C.Y. (2000) A 5-year study of the seroepidemiology of Klebsiella pneumoniae: high prevalence of capsular serotype K1 in Taiwan and implication for vaccine efficacy. J Infect Dis 181: 2075-2079. Fung, C.P., Chang, F.Y., Lee, S.C., Hu, B.S., Kuo, B.I., Liu, C.Y., Ho, M., and Siu, L.K. (2002) A global emerging disease of Klebsiella pneumoniae liver abscess: is serotype K1 an important factor for complicated endophthalmitis? Gut 50: 420-424. Gerlach, G.F., Clegg, S., and Allen, B.L. (1989) Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae. J Bacteriol 171: 1262-1270. Heinrichs, D.E., Monteiro, M.A., Perry, M.B., and Whitfield, C. (1998) The assembly system for the lipopolysaccharide R2 core-type of Escherichia coli is a hybrid of those found in Escherichia coli K-12 and Salmonella enterica. Structure and function of the R2 WaaK and WaaL homologs. J Biol Chem 273: 8849-8859. Herrero, M., de Lorenzo, V., and Timmis, K.N. (1990) Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol 172: 6557-6567. Hitchcock, P.J., and Brown, T.M. (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154: 269-277. Kenne, L., and Lindberg, B. (1983) Bacterial polysaccharides. In The polysaccharides. Vol. 2. Aspinall, G.O. (ed). New York: Academic Press, Inc., pp. 287-363. Ko, W.C., Paterson, D.L., Sagnimeni, A.J., Hansen, D.S., Von Gottberg, A., Mohapatra, S., Casellas, J.M., Goossens, H., Mulazimoglu, L., Trenholme, G., Klugman, K.P., McCormack, J.G., and Yu, V.L. (2002) Community-acquired Klebsiella pneumoniae bacteremia: global differences in clinical patterns. Emerg Infect Dis 8: 160-166. Lai, Y.C., Peng, H.L., and Chang, H.Y. (2003) RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level. J Bacteriol 185: 788-800. Lee, K.T., Wong, S.R., and Sheen, P.C. (2001) Pyogenic liver abscess: an audit of 10 years' experience and analysis of risk factors. Dig Surg 18: 459-465; discussion 465-456. Lindstrom, S.T., Healey, P.R., and Chen, S.C. (1997) Metastatic septic endophthalmitis complicating pyogenic liver abscess caused by Klebsiella pneumoniae. Aust N Z J Med 27: 77-78. Liu, Y.C., Cheng, D.L., and Lin, C.L. (1986) Klebsiella pneumoniae liver abscess associated with septic endophthalmitis. Arch Intern Med 146: 1913-1916. Ma, L.C., Fang, C.T., Lee, C.Z., Shun, C.T., and Wang, J.T. (2005) Genomic heterogeneity in Klebsiella pneumoniae strains is associated with primary pyogenic liver abscess and metastatic infection. J Infect Dis 192: 117-128. Mizuta, K., Ohta, M., Mori, M., Hasegawa, T., Nakashima, I., and Kato, N. (1983) Virulence for mice of Klebsiella strains belonging to the O1 group: relationship to their capsular (K) types. Infect Immun 40: 56-61. Nassif, X., Fournier, J.M., Arondel, J., and Sansonetti, P.J. (1989a) Mucoid phenotype of Klebsiella pneumoniae is a plasmid-encoded virulence factor. Infect Immun 57: 546-552. Nassif, X., Honore, N., Vasselon, T., Cole, S.T., and Sansonetti, P.J. (1989b) Positive control of colanic acid synthesis in Escherichia coli by rmpA and rmpB, two virulence-plasmid genes of Klebsiella pneumoniae. Mol Microbiol 3: 1349-1359. Nulsen, M.F., Finlay-Jones, J.J., and McDonald, P.J. (1986) T-lymphocyte involvement in abscess formation in nonimmune mice. Infect Immun 52: 633-636. Ofek, I., Kabha, K., Athamna, A., Frankel, G., Wozniak, D.J., Hasty, D.L., and Ohman, D.E. (1993) Genetic exchange of determinants for capsular polysaccharide biosynthesis between Klebsiella pneumoniae strains expressing serotypes K2 and K21a. Infect Immun 61: 4208-4216. Parke, D. (1990) Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137. Podschun, R., Sievers, D., Fischer, A., and Ullmann, U. (1993) Serotypes, hemagglutinins, siderophore synthesis, and serum resistance of Klebsiella isolates causing human urinary tract infections. J Infect Dis 168: 1415-1421. Podschun, R., and Ullmann, U. (1998) Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev 11: 589-603. Rahimian, J., Wilson, T., Oram, V., and Holzman, R.S. (2004) Pyogenic liver abscess: recent trends in etiology and mortality. Clin Infect Dis 39: 1654-1659. Rahn, A., Drummelsmith, J., and Whitfield, C. (1999) Conserved organization in the cps gene clusters for expression of Escherichia coli group 1 K antigens: relationship to the colanic acid biosynthesis locus and the cps genes from Klebsiella pneumoniae. J Bacteriol 181: 2307-2313. Saccente, M. (1999) Klebsiella pneumoniae liver abscess, endophthalmitis, and meningitis in a man with newly recognized diabetes mellitus. Clin Infect Dis 29: 1570-1571. Sawyer, R.G., Adams, R.B., May, A.K., Rosenlof, L.K., and Pruett, T.L. (1995) CD4+ T cells mediate preexposure-induced increases in murine intraabdominal abscess formation. Clin Immunol Immunopathol 77: 82-88. Seeto, R.K., and Rockey, D.C. (1996) Pyogenic liver abscess. Changes in etiology, management, and outcome. Medicine (Baltimore) 75: 99-113. Seydel, A., Gounon, P., and Pugsley, A.P. (1999) Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection. Mol Microbiol 34: 810-821. Simoons-Smit, A.M., Verweij-van Vught, A.M., and MacLaren, D.M. (1986) The role of K antigens as virulence factors in Klebsiella. J Med Microbiol 21: 133-137. Stevenson, G., Andrianopoulos, K., Hobbs, M., and Reeves, P.R. (1996) Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J Bacteriol 178: 4885-4893. Straus, D.C. (1987) Production of an extracellular toxic complex by various strains of Klebsiella pneumoniae. Infect Immun 55: 44-48. Tang, L.M., Chen, S.T., Hsu, W.C., and Lyu, R.K. (1999) Acute bacterial meningitis in adults: a hospital-based epidemiological study. Qjm 92: 719-725. Taylor, L.A., and Rose, R.E. (1988) A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K. Nucleic Acids Res 16: 358. Tzianabos, A.O., Chandraker, A., Kalka-Moll, W., Stingele, F., Dong, V.M., Finberg, R.W., Peach, R., and Sayegh, M.H. (2000) Bacterial pathogens induce abscess formation by CD4(+) T-cell activation via the CD28-B7-2 costimulatory pathway. Infect Immun 68: 6650-6655. Wacharotayankun, R., Arakawa, Y., Ohta, M., Tanaka, K., Akashi, T., Mori, M., and Kato, N. (1993) Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ. Infect Immun 61: 3164-3174. Wang, J.H., Liu, Y.C., Lee, S.S., Yen, M.Y., Chen, Y.S., Wang, J.H., Wann, S.R., and Lin, H.H. (1998) Primary liver abscess due to Klebsiella pneumoniae in Taiwan. Clin Infect Dis 26: 1434-1438. Wu, H. (1996) Biosynthesis of lipoproteins. In Escherichia Coli and Salmonella Typhimurium. Neidhardt, F. (ed). Washington, DC: ASM. Yang, C.C., Yen, C.H., Ho, M.W., and Wang, J.H. (2004) Comparison of pyogenic liver abscess caused by non-Klebsiella pneumoniae and Klebsiella pneumoniae. J Microbiol Immunol Infect 37: 176-184. 方啟泰 (2002) 造成肝膿瘍之侵襲性克雷伯氏肺炎桿菌菌株致病基因的研究. In 臨床醫學研究所. Vol. 博士 台北: 國立台灣大學醫學院, pp. 95. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34800 | - |
| dc.description.abstract | 克雷伯氏肺炎桿菌是革蘭氏陰性的桿菌，隸屬於腸內菌科，為一重要的伺機性感染的病原菌。近20年來，一種新型的侵襲性克雷伯氏肺炎桿菌感染逐漸躍升為一個全球性的新興重要社區感染症。最常見的臨床表現為原發性肝膿瘍合併菌血症及轉移性眼內炎或腦膜炎。菌株觀察發現高黏性型態和上述克雷伯氏肺炎桿菌的侵襲性感染有關；因此利用跳躍基因所建構的突變株庫找到一個與高黏性型態有關的基因，magA（mucoviscosity-associated gene A）； magA突變菌株失去對健康人的血清及嗜中性白血球的抗性，並失去致病力。合併比較莢膜血清型的統計發現，從帶有原發性肝膿瘍病人身上分離而得的42株臨床菌株中，35株為K1；而從不帶有原發性肝膿瘍病人身上分離而得的32株臨床菌株中，只有1株為K1（35/42 vs. 1/32， p<0.001）。此36株K1的菌株經由聚合酶鏈反應分析，皆帶有magA；其餘38株非K1的菌株則不帶有magA（36/36 vs. 0/38，p<0.0001）。序列分析magA的上下游區域，顯示magA位在莢膜生合成基因組中（galF至ugd共20 ORFs：open-reading frames）；其中，包含magA的9個ORFs（共10 kb）與MGH78578（K52）、Chedid（K2）的相對區域序列歧異度很大。針對NTUH-K2044此段區域其中4個基因作插入性突變，發現突變株失去了與抗K1血清凝集的反應，但若進行異位互補，則抗K1的凝集反應可以回復；此外，包含magA的11 kb區域（wzx至rfbP）以異位互補的方式送進5株非K1的克雷伯氏肺炎桿菌菌株可將其中2株的莢膜血清型轉為K1陽性。同時利用聚合酶鏈反應分析，認為此段11 kb區域中，wzx-gmd及wcaI都是莢膜血清型K1獨有的部份。因此我們認為magA位在25 kb的莢膜生合成基因組上，其中wzx至wcaI（包括magA）為莢膜血清型K1的基因決定區域，並與克雷伯氏肺炎桿菌造成原發性肝膿瘍有高度相關。 | zh_TW |
| dc.description.abstract | Klebsiella pneumoniae is a Gram-negative bacillus belonging to Enterobacteriace, which is a opportunistic pathogen. However, a new type of tissue-invasive K. pneumoniae infection has emerged over the past two decades. The common clinical symptoms represents primary liver abscess complicated with (or without) septic meningitis and endophthalmitis. Mucoviscosity of K. pneumoniae strains was hypothesized to be correlated with tissue-invasive infection by clinical manifestation. We constructed mutant library of NTUH-K2044 using transposon mutagenesis and screened for mucoviscosity-deficient mutants. A magA (mucoviscosity-associated gene A) gene was thus identified. magA mutant strain was susceptible to non-immune human serum as well as neutrophil phagocytosis and became avirulent. Analysis of magA and serotype revealed that thirty-five of 42 strains from patients with primary liver abscess were magA positive while only 1 of 32 non- primary liver abscess strains positive. All 36 magA-positive strains were serotype K1 and the 38 magA-negative strains were not (36/36 vs. 0/38, p<0.0001). Sequencing of magA-flanking region revealed a putative capsular polysaccharide synthesis (cps) region; this region was 25kb in length and contained 20 ORFs. Of them, there were 9 ORFs (10 kb) which were co-transcribed as part of an operon and different from both MGH78578 and Chedid strains. Mutation of 4 genes in this region turned the mutant strains into anti-K1 sero-negative. Trans-complementations restored K1 phenotype. The magA-flanking regions, wzx to rfbP, successively converted 2 of 5 non-K1 K. pneumoniae strains into K1 sero-positive by trans-complementation. Thus we concluded that the operon containing magA is responsible for capsular serotype K1 in K. pneumoniae. Several loci in the operon are unique determinants for K1 strains. These results may further help to the early detection and tracing the origin of this emerging infectious disease. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:34:51Z (GMT). No. of bitstreams: 1 ntu-94-F88445101-1.pdf: 2637284 bytes, checksum: 01b37b616d4596dc8923a44bf85ea2f4 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………………………. 1
英文摘要……………………………………………………………………. 2 第一章 緒論……………………………………………………………… 3 第二章 材料及方法………………………………………………………8 1. 細菌菌株及質體………………………………………………………..8 2. 拉絲測試………………………………………………………………..8 3. 建立克雷伯氏肺炎桿菌突變株庫…………………………………….8 4. 萃取克雷伯氏肺炎桿菌基因體DNA…………………………………9 5. 南方轉漬法……………………………………………………………..9 6. 萃取克雷伯氏肺炎桿菌total RNA…………………………………11 7. 反轉錄聚合酶鏈反應……………………………………………….12 8. 異位互補……………………………………………………………..14 9. 建立克雷伯氏肺炎桿菌突變株………………………………………14 10. 血清抗性試驗………………………………………………….…15 11. 補體沉積試驗…………………………………………………..…15 12. 吞噬作用試驗…………………………………………………..….16 13. 觀察脂多醣的O抗原模式…………………………………………17 14. 莢膜血清分型………………………………………………………18 第三章 結果…………………………………………………………………20 1. 建立克雷伯氏肺炎桿菌突變株庫…………………………………..20 2. 突變株多樣性測試………………………………………………….20 3. 突變株序列分析…………………………………………………….20 4. magA對先天免疫反應的影響………………………………………22 5. 經由生物資訊的方式了解MagA的生物功能……………………23 6. magA上下游序列分析………………………………………………25 7. magA與莢膜血清型的關係…………………………………………27 8. 莢膜血清型K1的基因決定區……………………………………..28 9. 莢膜生合成基因組轉錄單位的分析……………………………….29 第四章 總結與討論…………………………………………………………30 表目錄 表一 研究中使用的細菌菌株及載體……………………………………..36 表二 實驗中使用到的引子………………………………………………..37 表三 NTUH-K2044之莢膜生合成基因組中基因排列與功能分析…….38 表四 組織侵襲性與非組織侵襲性克雷伯氏肺炎桿菌菌株之莢膜血清型magA基因之分布…………………………………………………….39 圖目錄 圖一 建構克雷伯氏肺炎桿菌突變株示意圖……………………………....40 圖二 喪失黏性型態的克雷伯氏肺炎桿菌突變株在EMB培養基上與野生型NTUH-K2044的外觀比較……………………………………...…..41 圖三 萃取脂多醣觀察菌株間O抗原存在與否…………………………..42 圖四 比較Chedid、NTUH-K2044及MGH78578之莢膜生合成基因組序列……………………………………………………………………..43 圖五 利用對向免疫擴散法觀察各突變株莢膜血清型K1表現的情形…44 圖六 利用聚合酶鏈反應偵測wzx至rfbp中六個基因片段在K1與非K1克雷伯氏肺炎桿菌菌株中的分布…………………………………..45 圖七 利用對向免疫擴散法觀察wzx-rfbP及magA對非K1臨床菌株莢膜血清型的影響…………………………………………………………46 圖八 莢膜生合成基因組之轉錄單位的分析……………………………...47 第五章 參考文獻…………………………………………………………….48 第六章 附錄…………………………………………………………………55 圖A 利用南方墨點法分析突變株岐異度的結果…………………………55 圖 B magA基因對先天免疫反應的影響………………………………56 圖C 比較各菌株抵抗吞噬作用的能力……………………………………57 圖D MagA的功能性生物資訊學分析………………………………….58 圖 E 克雷伯氏肺炎桿菌莢膜血清型K1、K2、K5、K20、K54及K57之結構示意圖……………………………………………………………59 | |
| dc.language.iso | zh-TW | |
| dc.subject | 莢膜 | zh_TW |
| dc.subject | 克雷伯氏肺炎桿菌 | zh_TW |
| dc.subject | 組織侵襲性 | zh_TW |
| dc.subject | Klebsiella pneumoniae | en |
| dc.subject | tissue-invasive | en |
| dc.subject | mucoid | en |
| dc.subject | magA | en |
| dc.subject | K1 | en |
| dc.subject | capsule | en |
| dc.title | 組織侵襲性克雷伯氏肺炎桿菌中莢膜血清型K1的基因決定區 | zh_TW |
| dc.title | The cps Region Responsible for Capsular Serotype K1 in Klebsiella pneumoniae Causing Human Primary Pyogenic Liver Abscess | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 何漣漪,伍安怡,鄧述諄,楊偉勳 | |
| dc.subject.keyword | 克雷伯氏肺炎桿菌,莢膜,組織侵襲性, | zh_TW |
| dc.subject.keyword | Klebsiella pneumoniae,capsule,K1,magA,mucoid,tissue-invasive, | en |
| dc.relation.page | 59 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-01-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 未授權公開取用 | 2.58 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。