以線上光學檢測系統進行單寧酸之定量分析

Chien-Yu Chung; 鍾建育

請用此 Handle URI 來引用此文件： http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34712

完整後設資料紀錄

DC 欄位	值	語言
dc.contributor.advisor	陳力騏(Richie, Che)
dc.contributor.author	Chien-Yu Chung	en
dc.contributor.author	鍾建育	zh_TW
dc.date.accessioned	2021-06-13T06:24:11Z	-
dc.date.available	2006-01-26
dc.date.copyright	2006-01-26
dc.date.issued	2006
dc.date.submitted	2006-01-23
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Freitas. 2004. Flow nephelometric analysis of protein-tannin interactions. Analytica Chimica Acta 513, 97-101. 10. Chatterjee, S., R. Chowdhury, P. Bhattacharya. 2000. A mathematical analysis of the effect of pH and protein concentration on adsorption of proteins on immobilised tannin. Biochemical Engineering Journal 5, 77-82. 11. Corominas, B. G-T., J.V. Garcia Mateo, L. L. Zamora, J. M. Calatayud. 2002. Determination of tannic acid by direct chemiluminescence in a FIA assembly. Talanta 58, 1243-1251. 12. Cui, H., O. Li, R. Meng, H. Zhao, C. He. 1998. Flow injection analysis of tannic acid inhibited chemiluminescent detection. Analytic Chimica Acta 62 362, 151–155. 13. Dodeigne, C., L. Thunus. 2000. Chumiluminescence as diagnostic tool. A review. Talanta 51, 415-439. 14. Edilene, C. F., A. R. A. Nogueira. 2000. Vanillin-condensed tannin study using flow injection spectrophotometry. Talanta 51, 1-6. 15. Fickel, J., Ch. Pitra, B.A. Joest , R.R Hofmann. 1999. A novel method to evaluate the relative tannin-binding capacities of salivary proteins. Comparstive Biochemistry and Physiology, Part C 122, 225-229. 16. Freitas, V. D., E. Carvalho, N. Mateus. 2003. Study of carbohydrate influence on protein–tannin aggregation by nephelometry. Food Chemistry 81, 503–509. 17. Freitas, V. D., N. Mateus. 2001. Nephelometric study of salivary protein-tannin aggregates. Journal of Science Food Agriculture 82, 113-119. 18. George S., P. Brat, P. Alter, M. J. Amiot. 2005. Rapid Determination of Polyphenols and Vitamin C in Plant-Derived Products. Journal of Agricultural and Food Chemistry 53, 1370-1373. 19. Hagerman, A. E., L. G. Butler. 1988. Choosing appropriate methods and standards for assaying tannin. Journal of Chemical Ecology 15, 1975-1809. 20. Harvey, I. M.. 2001. Analysis of hydrolysable tannins. Animal Feed Science and Technology 91, 3-20. 21. Haslam, E.. 1996. Natural Polyphenols (Vegetable Tannins) as drugs: Possible Modes of Action. Journal of Natural Production 59, 205-215. 22. Hupkens, P., H. Boxma, J. Dokter. 1995. Tannic acid as a topical agent in burns: historical considerations and implications for new developments. Burns Vol. 21, No.1, pp.57-61. 23. Kilkowski, W. J., G. G. Gross. 1999. Color reaction of hydrolyzable tannins with Bradford reagent, Coomassie brilliant blue. Phytochemistry 51, 363-366. 24. Lau, O. W., S. F. Luk, H. L. Huang. 1989. Spectrophotometric Determination of Tannins in Tea and Beer Samples With Iron (III) and 1,10-Phenanthroline as Reagents. ANALYST 114, 631-633. 25. Lima, L., S. Olivares, F. Martines, J. Torres. D. D. L. Rosa. C. Sepulveda. 1998. Use of immobilized tannin adsorbent for removal of Cr(VI) from water. Journal of Radioanalytical and Nuclear Chemistry 231, 35-40. 26. Lin, H. C., P. C. Chen, T. J. Cheng, R. L. C. Chen. 2004. Formation of tannin-albumin nano-particles at neutral pH as measured by light scattering techniques. Analytical Biochemistry 325, 117-120. 27. Lu, Y., A. Bennick. 1998. Interaction of tannin with human salivary praline-rich proteins. Archives of Oral Biology 43, 717-728. 28. Luczaj, W., E. Skrzydlewska. 2005. Antioxidative properties of black tea. 63 Preventive Medicine 40. 910-918. 29. Maie, N., A. Behrens, H. Knicker, I. K. Knabner. 2003. Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves. Soil biology & Biochemistry 35, 577-589. 30. Mole, S., P. G. Waterman. 1987. A critical analysis of techniques for measuring tannins in ecological studies. Oecologia 2, 137-147. 31. Robbins, R. J.. 2003. Phenolic Acid in Foods: An Overview of Analytical Methodology. Journal of Agriculture Food Chemistry 51, 2866-2887. 32. Schofield, P., D.M. Mbugua, A.N. Pell. 2001. Analysis of condensed tannins: a review. Animal Feed Science and Technology 91, 21-40. 33. Silber, M. L., B. B. Davitt, R. F. Khairutdinov, J. K. Hurst. 1998. A Mathematical Model Describing Tannin–Protein Association. Analytical Biochemistry 263, 46–50. 34. Tanaka, T., I. Kouno. 2003. Oxidation of tea catechins: Chemical structures and reaction mechanism. FOOD SCIENCE AND TECHNOLOGY RESEACH 9, 128-133. 35. Varanka, Z., I. Rojik, I. Varanka, J. Nemcsok, M. Abraham. 2001. Biochemical and morphological changes in carp (Cyprinus carpio L.) liver following exposure to copper sulfate and tannic acid. Comparative Biochemistry and Physiology Part C 128, 467-478. 36. Xie, C., H. Cui. 2003. Detection of tannic acid at trace level in industrial wastewaters using a highly sensitive chemiluminescence method. Water Research 37, 233-237. 37. Yamamoto, T., L. R. Juneja, D. C. Chu, M. Kim. 1997. Chemistry and Applications of Green Tea. 38. Yebra, M. C., M. Gallego, M. Valcercal. 1995. Indirect flow-injection determination of tannins in wines and tea by atomic absorption spectrometry. Analytica Chimica Acta 308, 357-363. 39. Zhao, Y. B., M. L. Wen, S. Q. Liu, Z. H. Liu, W. D. Zhang, Y. Yao. C. Y. Wang. 1998. Microbial Sensor for Determination of Tannic Acid. Microchemical Journal 60, 201–209. 40. Zhou, Y., T. Nagaoka, F. Li, G. Zhu. 1999. Evaluation of luminol-H2O2-KIO4 chemiluminescence system and its application to hydrogen peroxide, glucose and ascorbic acid assays. Talanta 48, 461-467.
dc.identifier.uri	http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34712	-
dc.description.abstract	摘要本研究針對茶品中單寧酸總量檢測與茶品中單寧酸與蛋白質凝集造成澀味感受進行探討，分別建立了螢光消光流動系統與光散射流動系統。在螢光消光流動系統中，能有效避免維他命C 與溫度變化的干擾，並擁有對單寧酸高度選擇性與檢測靈敏等優點。在茶品實測的應用面上，本系統的線性範圍為濃度0.0-1.0 mg/ml，R2 ＝ 0.9928，且分析結果與日本公定法具有高度相關性（R2 ＝ 0.9803）。在光散射流動系統中，SDS 洗劑清洗程序能有效的改善訊號之背景值漂移與重複性不佳等實驗誤差，徹底清除吸附於管壁的單寧酸與BSA 之凝集分子，成功建立一套能即時檢測與即時清洗的線上感測系統。系統檢測下限為20 μg/ml，上限為300 μg/ml。此系統能有效以單寧酸與蛋白質的凝集情形得知澀味等生理活性。	zh_TW
dc.description.abstract	Two on-line systems have been setup in this research for determination of tannic acid content in green tea infusion and biological activation of protein and tannic acid aggregation in tea. In fluorescence quenching method with flow-injection system which has been applied for detection of real samples within a linear concentration range of 0.0-1.0 mg/ml and a R2 of 0.9928. The method is immune from the interference of the coexisting ascorbate in green tea infusion and temperature changing, and the analytical results were verified by Japanese official analytical method, ferrous tartrate method. In light scattering method with flow-injection system, experiment error such as drift of baseline and lacking of reproducibility can be solved effectively by cleaning procedure with SDS reagent for the effective removal of residual aggregation particles by tannic acid and BSA. Therefore, an on-line detection system with real time detection and cleaning ability have been setup successfully. The range of detection is 20-300 μg/ml, and astringent activation in tea can be known by aggregation of tannic acid and BSA	en
dc.description.provenance	Made available in DSpace on 2021-06-13T06:24:11Z (GMT). No. of bitstreams: 1 ntu-95-R92631015-1.pdf: 607999 bytes, checksum: 8bc7d4b0d8db8ea880b15168ba30b0be (MD5) Previous issue date: 2006	en
dc.description.tableofcontents	目錄封面····················································································································i 口試委員簽名單·······························································································ii 誌謝··················································································································iii 摘要··················································································································iv 英文摘要··········································································································· v 目錄圖目錄表目錄一、前言··········································································································· 1 二、文獻探討 2.1 單寧酸的化學結構··············································································· 3 2.2 單寧酸的定量法··················································································· 8 2.2.1 縮合單寧定量法··········································································· 8 2.2.2 水解單寧定量法········································································· 10 2.2.3 單寧總量測定法·················································································11 2.2.4 單寧酸生物活性測定法······································································12 II 2.2.5 單寧酸之流動注射分析法··································································13 2.2.6 本研究之實驗原理··············································································14 三、材料與方法 3.1 實驗藥品製備與實驗儀器································································· 18 3.1.1 實驗藥品····················································································· 18 3.1.2 緩衝液與藥品製備····································································· 18 3.1.3 儀器設備····················································································· 19 3.2 系統架設與實驗方法········································································· 20 3.2.1 螢光消光流動分析系統架設與實驗方法································· 20 3.2.2 光散射流動分析系統架設與實驗方法······································ 22 3.2.3 日本公定綠茶單寧分析法························································· 24 四、結果與討論 4.1 螢光消光法························································································· 25 4.1.1 系統典型響應與系統線性範圍················································· 25 4.1.2 最適化探討················································································· 28 4.1.2.1 不同濃度發光胺對檢測之影響········································· 28 4.1.2.2 pH 值最適化探討································································ 30 4.1.3 溫度與干擾物影響····································································· 32 4.1.3.1 溫度對消光效應之影響····················································· 32 4.1.3.2 維生素C 之影響································································ 34 III 4.1.4 市售綠茶支單寧酸檢測····························································· 36 4.1.4.1 螢光消光法與日本公定法的比對····································· 36 4.1.4.2 茶葉浸置時間與單寧酸釋放量之探討····························· 39 4.2 光散射法····························································································· 41 4.2.1 非自動化檢測系統觀察單寧酸與BSA 之凝集過程················· 41 4.2.2 訊號漂移效應與改善方案·························································· 44 4.2.3 實驗參數探討············································································· 48 4.2.3.1 清洗管長最適化································································· 48 4.2.3.2 系統之典型響應································································· 49 4.2.3.3 BSA 濃度對檢測所造成之影響········································· 51 4.2.3.4 pH 值對檢測所造成之影響················································ 55 4.2.4 茶品實測····················································································· 56 五、結論········································································································· 59 六、參考文獻································································································· 61
dc.language.iso	zh-TW
dc.subject	單寧酸	zh_TW
dc.subject	流動分析系統	zh_TW
dc.subject	螢光消光	zh_TW
dc.subject	綠茶	zh_TW
dc.subject	光散射	zh_TW
dc.subject	牛血清蛋白	zh_TW
dc.subject	澀味	zh_TW
dc.subject	tannic acid	en
dc.subject	green tea	en
dc.subject	quenching	en
dc.subject	fluorescence	en
dc.subject	flow-injection	en
dc.subject	astringency	en
dc.subject	BSA	en
dc.title	以線上光學檢測系統進行單寧酸之定量分析	zh_TW
dc.title	Determination of tannic acid based on on-line optical detection system	en
dc.type	Thesis
dc.date.schoolyear	94-1
dc.description.degree	碩士
dc.contributor.oralexamcommittee	鄭宗記(Tzong-Jih Cheng),李允中(Yeun-Chung Lee),彭錦樵(Jinchyau Peng)
dc.subject.keyword	流動分析系統,單寧酸,螢光消光,綠茶,光散射,牛血清蛋白,澀味,	zh_TW
dc.subject.keyword	flow-injection,tannic acid,fluorescence,quenching,green tea,BSA,astringency,	en
dc.relation.page	63
dc.rights.note	有償授權
dc.date.accepted	2006-01-24
dc.contributor.author-college	生物資源暨農學院	zh_TW
dc.contributor.author-dept	生物產業機電工程學研究所	zh_TW
顯示於系所單位：	生物機電工程學系

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