請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34625完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 丁詩同 | |
| dc.contributor.author | Ya-Huei Ko | en |
| dc.contributor.author | 柯雅慧 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:19:00Z | - |
| dc.date.available | 2007-02-06 | |
| dc.date.copyright | 2006-02-06 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-01-26 | |
| dc.identifier.citation | Amemiya-Kudo, M. et al. 2002. Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes. J Lipid Res 43: 1220-1235.
Assaf, S. et al. 2003. Cloning of cDNA encoding the nuclear form of chicken sterol response element binding protein-2 (SREBP-2), chromosomal localization, and tissue expression of chicken SREBP-1 and -2 genes. Poult Sci 82: 54-61. Baulcombe, D. C. 1999. Gene silencing: RNA makes RNA makes no protein. Curr Biol 9: R599-601. Belloir, B., C. Diot, and M. Douaire. 1997. Triglyceride synthesis and secretion and lipogenesis implicated gene expression in the chicken hepatocarcinoma cell line LMH. Eur J Cell Biol 74: 246-250. Bernstein, E., A. A. Caudy, S. M. Hammond, and G. J. Hannon. 2001. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409: 363-366. Briggs, M. R., C. Yokoyama, X. Wang, M. S. Brown, and J. L. Goldstein. 1993. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. I. Identification of the protein and delineation of its target nucleotide sequence. J Biol Chem 268: 14490-14496. Brown, M. S., and J. L. Goldstein. 1997. The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89: 331-340. Bujo, H., M. Hermann, K. A. Lindstedt, J. Nimpf, and W. J. Schneider. 1997. Low density lipoprotein receptor gene family members mediate yolk deposition. J Nutr 127: 801S-804S. Caplen, N. J., S. Parrish, F. Imani, A. Fire, and R. A. Morgan. 2001. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci U S A 98: 9742-9747. Chesnutt, C., and L. Niswander. 2004. Plasmid-based short-hairpin RNA interference in the chicken embryo. Genesis 39: 73-78. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159. DeBose-Boyd, R. A. et al. 1999. Transport-dependent proteolysis of SREBP: Relocation of site-1 protease from Golgi to er obviates the need for SREBP transport to Golgi. Cell 99: 703-712. Donaldson, W. E. 1990. Lipid metabolism in liver of chicks: response to feeding. Poult Sci 69: 1183-1187. Dooley, K. A., M. K. Bennett, and T. F. Osborne. 1999. A critical role for cAMP response element-binding protein (CREB) as a co-activator in sterol-regulated transcription of 3-hydroxy-3-methylglutaryl coenzyme a synthase promoter. J Biol Chem 274: 5285-5291. Dridi, S., J. Buyse, E. Decuypere, and M. Taouis. 2005. Potential role of leptin in increase of fatty acid synthase gene expression in chicken liver. Domest Anim Endocrinol 29: 646-660. Duncan, E. A., M. S. Brown, J. L. Goldstein, and J. Sakai. 1997. Cleavage site for sterol-regulated protease localized to a Leu-Ser bond in the lumenal loop of sterol regulatory element-binding protein-2. J Biol Chem 272: 12778-12785. Duncan, E. A., U. P. Dave, J. Sakai, J. L. Goldstein, and M. S. Brown. 1998. Second-site cleavage in sterol regulatory element-binding protein occurs at transmembrane junction as determined by cysteine panning. J Biol Chem 273: 17801-17809. Eberle, D., B. Hegarty, P. Bossard, P. Ferre, and F. Foufelle. 2004. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 86: 839-848. Elbashir, S. M., J. Martinez, A. Patkaniowska, W. Lendeckel, and T. Tuschl. 2001. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. Embo J 20: 6877-6888. Fire, A. et al. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811. Goldman, M. J., D. W. Back, and A. G. Goodridge. 1985. Nutritional regulation of the synthesis and degradation of malic enzyme messenger RNA in duck liver. J Biol Chem 260: 4404-4408. Goodridge, A. G. 1968. Citrate-cleavage enzyme, 'malic' enzyme and certain dehydrogenases in embryonic and growing chicks. Biochem J 108: 663-666. Goodridge, A. G., and E. G. Ball. 1967. Lipogenesis in the pigeon: In vivo studies. Am J Physiol 213: 245-249. Griffin, H. D., K. Guo, D. Windsor, and S. C. Butterwith. 1992. Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. J Nutr 122: 363-368. Guo, S., and K. J. Kemphues. 1995. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell 81: 611-620. Handschin, C. et al. 2004. The evolution of drug-activated nuclear receptors: one ancestral gene diverged into two xenosensor genes in mammals. Nucl Recept 2: 7. Hermier, D. 1997. Lipoprotein metabolism and fattening in poultry. J Nutr 127: 805S-808S. Hillgartner, F. B., T. Charron, and K. A. Chesnut. 1996. Alterations in nutritional status regulate acetyl-CoA carboxylase expression in avian liver by a transcriptional mechanism. Biochem J 319 ( Pt 1): 263-268. Holen, T., M. Amarzguioui, M. T. Wiiger, E. Babaie, and H. Prydz. 2002. Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor. Nucleic Acids Res 30: 1757-1766. Horton, J. D., Y. Bashmakov, I. Shimomura, and H. Shimano. 1998. Regulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Proc Natl Acad Sci U S A 95: 5987-5992. Hua, X., J. Wu, J. L. Goldstein, M. S. Brown, and H. H. Hobbs. 1995. Structure of the human gene encoding sterol regulatory element binding protein-1 (SREBF) and localization of SREBF1 and SREBF2 to chromosomes 17p11.2 and 22q13. Genomics 25: 667-673. Hua, X. et al. 1993. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc Natl Acad Sci U S A 90: 11603-11607. Jorgensen, R. 1990. Altered gene expression in plants due to trans interactions between homologous genes. Trends Biotechnol 8: 340-344. Kawaguchi, T., K. Nomura, Y. Hirayama, and T. Kitagawa. 1987. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res 47: 4460-4464. Kennerdell, J. R., and R. W. Carthew. 1998. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 95: 1017-1026. Kim, D. H. et al. 2005. Synthetic dsRNA dicer substrates enhance RNAi potency and efficacy. Nat Biotechnol 23: 222-226. Latasa, M. J., M. J. Griffin, Y. S. Moon, C. Kang, and H. S. Sul. 2003. Occupancy and function of the -150 sterol regulatory element and -65 E-box in nutritional regulation of the fatty acid synthase gene in living animals. Mol Cell Biol 23: 5896-5907. Legrand, P., D. Catheline, J. M. Hannetel, and P. Lemarchal. 1994. Stearoyl-CoA desaturase activity in primary culture of chicken hepatocytes. Influence of insulin, glucocorticoid, fatty acids and cordycepin. Int J Biochem 26: 777-785. Magana, M. M., S. H. Koo, H. C. Towle, and T. F. Osborne. 2000. Different sterol regulatory element-binding protein-1 isoforms utilize distinct co-regulatory factors to activate the promoter for fatty acid synthase. J Biol Chem 275: 4726-4733. Magana, M. M., and T. F. Osborne. 1996. Two tandem binding sites for sterol regulatory element binding proteins are required for sterol regulation of fatty-acid synthase promoter. J Biol Chem 271: 32689-32694. Miyazaki, M. et al. 2004. Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and -independent mechanisms. J Biol Chem 279: 25164-25171. Morioka, H., G. E. Tennyson, and V. M. Nikodem. 1988. Structural and functional analysis of the rat malic enzyme gene promoter. Mol Cell Biol 8: 3542-3545. Nohturfft, A., M. S. Brown, and J. L. Goldstein. 1998. Topology of SREBP cleavage-activating protein, a polytopic membrane protein with a sterol-sensing domain. J Biol Chem 273: 17243-17250. Nykanen, A., B. Haley, and P. D. Zamore. 2001. ATP requirements and small interfering RNA structure in the rna interference pathway. Cell 107: 309-321. O'Hea, E. K., and G. A. Leveille. 1969. Lipid biosynthesis and transport in the domestic chick (Gallus domesticus). Comp Biochem Physiol 30: 149-159. Paddison, P. J., A. A. Caudy, E. Bernstein, G. J. Hannon, and D. S. Conklin. 2002. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev 16: 948-958. Richards, M. P. et al. 2003. Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens. J Nutr 133: 707-715. Sakai, J. et al. 1997. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein. J Biol Chem 272: 20213-20221. SAS. 2002. SAS procedure guide for personal computers. Version 6th Ed. SAS Institue Inc. Cary. NC. U.S.A. Sato, K., A. Ohuchi, S. H. Sook, M. Toyomizu, and Y. Akiba. 2003. Changes in mRNA expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase and cholesterol 7 alpha-hydroxylase in chickens. Biochim Biophys Acta 1630: 96-102. Sato, R. et al. 1994. Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1). J Biol Chem 269: 17267-17273. Shimano, H. et al. 1988. Down-regulation of hepatic LDL receptor protein and messenger RNA in fasted rabbits. J Biochem (Tokyo) 104: 712-716. Shimano, H. et al. 1996. Overproduction of cholesterol and fatty acids causes massive liver enlargement in transgenic mice expressing truncated SREBP-1a. J Clin Invest 98: 1575-1584. Shimano, H. et al. 1997a. Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J Clin Invest 99: 846-854. Shimano, H. et al. 1997b. Elevated levels of SREBP-2 and cholesterol synthesis in livers of mice homozygous for a targeted disruption of the SREBP-1 gene. J Clin Invest 100: 2115-2124. Shimano, H. et al. 1999. Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. J Biol Chem 274: 35832-35839. Shimomura, I., H. Shimano, J. D. Horton, J. L. Goldstein, and M. S. Brown. 1997. Differential expression of exons 1a and 1c in mrnas for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells. J Clin Invest 99: 838-845. Simpson, C. F., and R. H. Harms. 1983. Aortic atherosclerosis in nonlaying hens with fatty liver syndrome. Avian Dis 27: 652-659. Siperstein, M. D., and V. M. Fagan. 1964. Studies on the feed-back regulation of cholesterol synthesis. Adv Enzyme Regul 2: 249-264. Speake, B. K., A. M. Murray, and R. C. Noble. 1998. Transport and transformations of yolk lipids during development of the avian embryo. Prog Lipid Res 37: 1-32. Stark, J. J. et al. 1998. Interferon-alpha and chemohormonal therapy for patients with advanced melanoma: Final results of a phase I-II study of the Cancer Biotherapy Research Group and the Mid-Atlantic Oncology Program. Cancer 82: 1677-1681. Stoudemire, J. B., G. Renaud, D. M. Shames, and R. J. Havel. 1984. Impaired receptor-mediated catabolism of low density lipoproteins in fasted rabbits. J Lipid Res 25: 33-39. Sui, G. et al. 2002. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A 99: 5515-5520. Tu, D. C., M. L. Batten, K. Palczewski, and R. N. Van Gelder. 2004. Nonvisual photoreception in the chick iris. Science 306: 129-131. Wang, X. et al. 1993. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. I. Purification and characterization. J Biol Chem 268: 14497-14504. Wianny, F., and M. Zernicka-Goetz. 2000. Specific interference with gene function by double-stranded RNA in early mouse development. Nat Cell Biol 2: 70-75. Yang, T. et al. 2002. Crucial step in cholesterol homeostasis: Sterols promote binding of SCAP to insig-1, a membrane protein that facilitates retention of SREBPs in er. Cell 110: 489-500. Yen, C. F. et al. 2005. Cloning and expression of the genes associated with lipid metabolism in Tsaiya ducks. Poult Sci 84: 67-74. Yin, L., Y. Zhang, and F. B. Hillgartner. 2002. Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes. J Biol Chem 277: 19554-19565. Yokoyama, C. et al. 1993. Srebp-1, a basic-helix-loop-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell 75: 187-197. Zamore, P. D., T. Tuschl, P. A. Sharp, and D. P. Bartel. 2000. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101: 25-33. Zhang, Y., and F. B. Hillgartner. 2004. Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens. J Nutr 134: 2205-2210. Zhang, Y., L. Yin, and F. B. Hillgartner. 2003. SREBP-1 integrates the actions of thyroid hormone, insulin, cAMP, and medium-chain fatty acids on ACCalpha transcription in hepatocytes. J Lipid Res 44: 356-368. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34625 | - |
| dc.description.abstract | 本試驗目的為探討固醇調節區域結合蛋白（Sterol regulatory element binding protein, SREBP）在雞隻中對於脂質新生成及膽固醇合成代謝基因的調控功能。本試驗分為兩部分進行，試驗一：將16隻7週齡肉雞隨機分成兩組，每組8隻，一組禁食24小時（禁食組），一組禁食24小時後給予任食5小時（飽食組），以即時定量聚合 | zh_TW |
| dc.description.abstract | To study the role of sterol regulatory element binding-proteins (SREBPs) in lipogenesis and cholesterol synthesis in the chicken, two experiments were carried out. First, 16 seven-week-old broilers were allocated into 2 groups treated with either fasted for 24 h or refed for 5 h after a 24 h-fast. The mRNA concentrations for SREBPs and other lipogenic genes in the liver were determined by quantitative real-time PCR. The hepatic mRNA relative abundance of lipogenic genes and genes in cholesterol synthesis were significantly greater (p<0.001) in the refed than the fasted broilers. Similar results were demonstrated with northern analysis technique. The data suggest that in the livers of fasted broilers, the lipogenesis and cholesterogenesis activities were inhibited. The data also demonstrated that the expression of lipogenic genes coordinate well as a group during the refed period. Second, three small interfering RNA(siRNA) oligonucleotides against the SREBP1 were designed to be used in transfecting chicken hepatocarcinoma cell line LMH. One of the 3 siRNAs effectively reduced SREBP1 mRNA concentration (p<0.01). The acetyl coenzyme A carboxylase | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:19:00Z (GMT). No. of bitstreams: 1 ntu-95-R92626012-1.pdf: 3046360 bytes, checksum: c95141d9819b776d5ec813d3870bb28d (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 目次
中文摘要 1 英文摘要 2 緒言 3 一、固醇調節區域結合蛋白 6 二、核糖核酸干擾 10 試驗目的 13 試驗一、雞隻禁食-飽食試驗 14 結果 21 討論 27 試驗二、利用肝細胞培養測定SREBP之功能 29 結果 34 討論 37 總結 39 參考文獻 40 附錄一、組織及細胞RNA之萃取 45 附錄二、甲醛變性膠體電泳 48 附錄三、反轉錄反應 48 附錄四、LMH細胞培養 50 附錄五、siRNA轉染 52 表次 表一、即時聚合 | |
| dc.language.iso | zh-TW | |
| dc.subject | 脂質新生成 | zh_TW |
| dc.subject | 固醇調結區域結合蛋白 | zh_TW |
| dc.subject | 肝臟 | zh_TW |
| dc.subject | 雞隻 | zh_TW |
| dc.subject | lipogenesis | en |
| dc.subject | sterol regulatory element binding protein | en |
| dc.subject | liver | en |
| dc.subject | chicken | en |
| dc.title | 雞隻固醇調節區域結合蛋白之表現及其對脂質生合成基因之調控 | zh_TW |
| dc.title | The expression of sterol regulatory element binding protein and its function on regulating the expression of lipogenic genes in the chicken | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 歐柏榮,陳珠亮,唐品琦 | |
| dc.subject.keyword | 雞隻,肝臟,固醇調結區域結合蛋白,脂質新生成, | zh_TW |
| dc.subject.keyword | chicken,liver,sterol regulatory element binding protein,lipogenesis, | en |
| dc.relation.page | 53 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-01-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 畜產學研究所 | zh_TW |
| 顯示於系所單位： | 動物科學技術學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-95-1.pdf 未授權公開取用 | 2.97 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。