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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34449完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張正憲 | |
| dc.contributor.author | Chia-Ming Chen | en |
| dc.contributor.author | 陳嘉銘 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:09:09Z | - |
| dc.date.available | 2007-06-20 | |
| dc.date.copyright | 2006-06-20 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-05-19 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34449 | - |
| dc.description.abstract | 本文是以原子力顯微鏡探討單一生物分子之間作用力為主，內容分成一量測Anti-Human IgG1與Human IgG1之間的解離力，探討解離力與壓入深度以及停留時間的關係，比較下壓時間與停留時間對解離力的影響，從數據中知道到了停留時間十秒時仍未有穩定的解離力出現，可見構形仍然在持續改變著。另外研究蛋白質拉伸的實驗，實驗樣本為丙胺酸(poly-alanine)，探討去摺疊力與實驗環境酸鹼度以及停留時間的關係，並將實驗所得的力-距離曲線與Worm Like Chain model做結合，如此可以進而求得丙胺酸的抗彎長度(persistence length)與實驗環境酸鹼度的關係，並與圓旋光光譜儀的實驗結果最比較，實驗結果證實不同酸鹼溶液下去摺疊力的大小與該胺基酸的pKa值息息相關。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:09:09Z (GMT). No. of bitstreams: 1 ntu-95-R92543057-1.pdf: 1867001 bytes, checksum: ec105c914bc9364a3f4e98feae984ab7 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 第一章 序論 1
1-1 前言 1 1-2文獻回顧 3 1-2-1 蛋白質拉伸實驗 3 1-2-2 抗原-抗體解離力實驗 7 1-3儀器介紹與比較 9 1-4 研究動機 12 第二章 實驗原理與方法 13 2-1 原子力顯微鏡基本架構 13 2-2 原子力顯微鏡的量測模式 16 2-2-1 力-距離曲線 18 2-2-2 比例常數量測 20 2-2-3 校正懸臂樑彈性常數 21 2-3 生物樣本製備與介紹 23 2-3-1 藥品配製 23 2-3-2 樣本製作與針尖修飾 23 2-3-2-1丙胺酸修飾 23 2-3-2-2 Human IgG1 and Anti-Human IgG1修飾 26 2-4 理論模型 29 2-4-1 Worm Like Chain model 分析 29 2-4-2 高斯分佈 38 2-5 力的量測與判讀 39 2-5-1 力的量測 39 2-5-2 接觸時間 42 第三章 實驗結果 44 3-1 抗體-抗原解離力實驗 44 3-2 蛋白質拉伸實驗 48 第四章 結論與展望 57 4-1 結論 57 4-2 未來展望 58 參考文獻 59 附錄A. 63 附錄B. 66 附錄C. 70 | |
| dc.language.iso | zh-TW | |
| dc.subject | 解離力 | zh_TW |
| dc.subject | 停留時間 | zh_TW |
| dc.subject | 去摺疊力 | zh_TW |
| dc.subject | Waiting time | en |
| dc.subject | Unfolding force | en |
| dc.subject | Unbinding force | en |
| dc.title | 停留時間對抗體-抗原解離力與丙胺酸去摺疊力之影響及酸鹼值對丙胺酸摺疊程度之探討 | zh_TW |
| dc.title | The Effect of Waiting time of Unbinding Force of IgG Anti-IgG and Unfolding force of Poly-Alanine. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 林世明 | |
| dc.contributor.oralexamcommittee | 吳光鍾,趙聖德 | |
| dc.subject.keyword | 解離力,去摺疊力,停留時間, | zh_TW |
| dc.subject.keyword | Unbinding force,Unfolding force,Waiting time, | en |
| dc.relation.page | 75 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-05-22 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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