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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
| dc.contributor.author | Ling-Chih Chien | en |
| dc.contributor.author | 簡苓芝 | zh_TW |
| dc.date.accessioned | 2021-06-13T04:46:32Z | - |
| dc.date.available | 2006-07-21 | |
| dc.date.copyright | 2006-07-21 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-17 | |
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'Molecular Recognition at Model Organic Interfaces: Electrochemical Discrimination Using Self-Assembled Monolayers Modified via the Fusion of Phospholipid Vesicles.' Langmuir 19.23 (2003): 9781-91. [19] Carey M. Merritt and James W. Winkelman. 'Electrochemical Method for Measuring C-Reactive Protein Using Crown Ether-Phosphate Ester Ionophores.' Analytical Chemistry 61.21 (1989): 2362-65. [20] Christopher E. D. Chidsey et al. 'Coadsorption of Ferrocene- Terminated and Unsubstituted Alkanethiols on Gold: Electroactive Self-Assembled Monolayers.' Journal of the American Chemical Society 112.11 (1990): 4301-6. [21] William P. Castelli. 'Lipids Risk Factors and Ischemic Heart Disease.' Atherosclerosis 124(Suppl) (1996):S1-S9. [22] Darren Thompson, Mark B. Pepys, and Steve P. Wood. 'The Physiological Structure of Human C-Reactive Protein and Its Complex with Phosphocholine.' Structure with Folding & Design 7.2 (1999): 169-77. [23] John E. Volanakis. 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Thermochimica Acta 334.1-2 (1999): 97-106. [34] Emil C. Gotschlich and Gerald M. Edelman. 'Binding Properties and Specificity of C-Reactive Protein.' The Proceedings of the National Academy of Sciences of the USA 57.3 (1967): 706-12. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33545 | - |
| dc.description.abstract | 電化學是對化學相關反應的研究，電化學法是一種快速簡便且成本低廉的方法，其特性可以運用在經過二茂鐵烷基硫醇分子修飾的電極來做為檢測蛋白質之感測端。本研究中以含二茂鐵尾端之烷基硫醇分子和脂質單層組成複合雙層仿生修飾電極，依此設計所製造出之工作電極以電化學檢測法用來作為仿生C反應蛋白感測器。此實驗之目的主要為驗證尾端含二茂鐵之烷基硫醇分子與含磷化膽鹼基之脂質單層所組成的仿生混合雙層膜是否可以執行C反應蛋白的檢測。不同莫耳分率之含二茂鐵分子溶液與無取代硫醇分子溶液混合所形成之自組裝單分子層可以操控其表面親疏水性及電子傳導性，尾端有氧化還原能力之二茂鐵分子層可以在分子辨識時提供分子內電子傳遞所需的能量。結果顯示浸泡含ferrocenylundecanethiol及undecanethiol之疏水性混合單分子溶液的金膜電極，對電極之電子傳遞能力較有幫助；且吸附磷脂質後，電極上之電活性分子會被阻礙，而引起電流的改變，則此仿生層之設計是可以用來量測C反應蛋白。其電化學性質和電極之電子傳遞能力也可以循環伏安法來定性，電流與濃度經線性迴歸後，其趨勢線與實驗資料之間的決定係數為0.8478。 | zh_TW |
| dc.description.abstract | Electrochemistry is principally the study of chemical reactions. This feature makes the study of protein via ferrocene-terminated alkanethiol modified SAM a feasible plan. In this study, biomimic modified gold electrode with ferrocene-terminated alkanethiol and lipid complex layer is designed and fabricated to serve as biomimic C-reactive protein sensor using electrochemical determination. The scope of this research is to know whether ferrocen-terminated SAM and hydrogenated phosphocholine biomimic hybrid bilayer could be used to perform C-reactive protein detection. With different mole fraction of ferrocene-terminated alkanethiol mixed with unsubstituted alkanethiol to manipulate the surface hydrophilicity and electro-conductivity, this redoxactive layer may give appropriate electron transfer ability of interconversion of electrical energy during molecular recognition.
The result shows that mixed hydrophobic SAM, which consists of ferrocenylundecanethiol and undecanethiol formed by soaking gold substrates in ethanol solutions of these two compounds, can increase electrode electron transfer ability. And after coating phospholipids, the electroactive molecules are hindered from the electrode, thus this kind of biomimic layer design can be used to measure CRP. Its electrochemical property and the changes of electrode’s electron transfer ability are characterized by cyclic voltammetry. The output of linear regression between induced current and concentration shows a value of R squared of 0.8478 which implies a good linearity. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T04:46:32Z (GMT). No. of bitstreams: 1 ntu-95-R92548014-1.pdf: 1565831 bytes, checksum: 520e263c335c28b97335b37c6c443417 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | Table of Contents
Acknowledgements i 中文摘要 ii Abstract iii Table of Contents iv List of Tables and Figures vi List of Abbreviations and Symbols viii Chapter 1. Introduction 1 1.1 Motivation 1 1.1.1 Cardiovascular Disease and Risk Factors 3 1.1.2 Assays for Measuring Plasma Lipids 4 1.1.3 Inflammatory Markers 5 1.1.4 C-reactive Protein in Blood and Its Determination 7 1.2 Electrochemical Biosensor 9 1.3 Objective 11 1.4 Importance 12 Chapter 2. Fundamentals of Experiments 14 2.1 C-reactive Protein Characteristics 14 2.1.1 Structure 14 2.1.2 Biological Functions 15 2.2 Characters of Biomimic Surface 18 2.3 Hybrid Layer Mechanism 19 2.3.1 Alkylthiol Self-Assembled Monolayer 19 2.3.2 Hybrid Bilayer 21 2.4 Possible Mechanism for CRP Detection 22 2.5 Electrochemical Method 23 Chapter 3. Experimental Procedure 28 3.1 Materials 28 3.2 Hybrid Bilayer Preperation 30 3.3 Electrochemistry Measurements 34 Chapter 4. Results and Discussion 38 4.1 Verification of Modified Electrode 38 4.2 Electrode Property Identification 40 4.3 Effecting Factors of Fabricated Electrodes 46 4.4 C-reactive Protein Detection 54 Chapter 5. Conclusion 61 References 62 | |
| dc.language.iso | en | |
| dc.subject | 循環伏安法 | zh_TW |
| dc.subject | C反應蛋白 | zh_TW |
| dc.subject | 自組裝二茂鐵烷基硫醇分子 | zh_TW |
| dc.subject | cyclic voltammetry | en |
| dc.subject | self-assembled ferrocene-terminated alkanethiol | en |
| dc.subject | C-reactive protein | en |
| dc.title | 仿生電活性奈米組裝分子多層之設計於高靈敏度C反應蛋白之電化學檢測 | zh_TW |
| dc.title | The Electrochemical Detection of high-sensitivity C-Reactive Protein Based on Biomimic Design of Electroactive Nanoassembly Multilayers | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 何國川(Kuo-Chuan Ho),劉言彬(Yen-Bin Liu),楊龍杰(Lung-Jieh Yang) | |
| dc.subject.keyword | C反應蛋白,自組裝二茂鐵烷基硫醇分子,循環伏安法, | zh_TW |
| dc.subject.keyword | C-reactive protein,self-assembled ferrocene-terminated alkanethiol,cyclic voltammetry, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-18 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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