靈芝、樟芝、裂褶菌多醣體對大白鼠自然殺手細胞與細胞素的影響

Abstract

本研究分別將靈芝、樟芝、裂褶菌三種不同菇蕈類所萃取的β-葡聚多醣體（β-glucan），以口服方式持續給予大白鼠一個月，並觀察對於大白鼠脾臟中自然殺手細胞毒殺能力的影響，以及檢測血液中細胞素的含量變化。結果發現靈芝、裂褶菌多醣體餵食組自然殺手細胞的毒殺能力均比對照組高且有顯著差異（P＜0.05），而樟芝多醣體餵食組則在前三週顯著高於對照組，第四週則與對照組沒有顯著差異（P＜0.05）。在測試血液中細胞素的實驗中，餵食三種不同多醣體均可顯著提升IFN-γ在血液中的含量（P＜0.05）；靈芝、裂褶菌多醣體餵食組血液中IL-2的含量顯著高於對照組（P＜0.05），樟芝多醣體餵食組則與對照組沒有顯著差異（P＜0.05）。靈芝、裂褶菌多醣體餵食組血液中IL-12的含量則分別在第6、第3天有對大值且顯著高於對照組（P＜0.05），樟芝多醣體餵食組則與對照組沒有顯著差異。TNF-α在血液中的含量方面，靈芝、裂褶菌餵食組分別在第6、第10天顯著高於對照組（P＜0.05），樟芝多醣體餵食組則與對照組沒有顯著差異。

In this study, Wistar rats were fed with three differentβ-glucan extracted from Ganoderma lucidum, Antrodia camphorata, and Schizophyllum commune. After 1 month experimental period, rats were sacrificed for the activity of natural killer cells and cytokine analysis. The result demonstrated that the activity of natural killer cells in rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher than control group（P＜0.05）. The activity of natural killer cells in rats fedβ-glucan extracted from Antrodia camphorata was significantly higher than control group in the 1st three weeks. In addition, IFN-γof rats fed different β-glucan were significantly higher than control group（P＜0.05）. IL-2 of rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher than control group（P＜0.05）. IL-2 of rats fedβ-glucan extracted from Antrodia camphorata had no significant（P＜0.05）different with the control group. IL-12 of rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher than control group（P＜0.05）on the 6th and 3rd day respectively, and IL-12 of rats fed β-glucan extracted from Antrodia camphorata had no significant（P＜0.05）different with the control group. TNF-αof rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher compared to that of the control rats（P＜0.05）on the 6th and 10th day respectively, and that of rats fed β-glucan extracted from Antrodia camphorata had no significant（P＜0.05）different with the control group.