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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 莊寧寧 | |
| dc.contributor.author | Jin-Wen Huang | en |
| dc.contributor.author | 黃勁文 | zh_TW |
| dc.date.accessioned | 2021-06-13T04:23:20Z | - |
| dc.date.available | 2006-07-25 | |
| dc.date.copyright | 2006-07-25 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-23 | |
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Usacheva, A., Smith, R., Minshall, R., Baida, G., Seng, S., Croze, E., & Colamonici, O. (2001). The WD motif-containing protein receptor for activated protein kinase C (RACK1) is required for recruitment and activation of signal transducer and activator of transcription 1 through the type I interferon receptor. J Biol Chem, 276(25), 22948-22953. Villena, J., Berndt, C., Granes, F., Reina, M., & Vilaro, S. (2003). Syndecan-2 expression enhances adhesion and proliferation of stably transfected Swiss 3T3 cells. Cell Biol Int, 27(12), 1005-1010. Won, M., Park, S. K., Hoe, K. L., Jang, Y. J., Chung, K. S., Kim, D. U., Kim, H. B., & Yoo, H. S. (2001). Rkp1/Cpc2, a fission yeast RACK1 homolog, is involved in actin cytoskeleton organization through protein kinase C, Pck2, signaling. Biochem Biophys Res Commun, 282(1), 10-15. Yamaguchi, Y., & Irie, F. (2002). [Roles of Eph receptors and the cell surface heparan sulfate proteoglycan syndecan-2 in dendritic spine morphogenesis]. Seikagaku, 74(5), 391-395. Yarwood, S. J., Steele, M. R., Scotland, G., Houslay, M. D., & Bolger, G. B. (1999). The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem, 274(21), 14909-14917. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33054 | - |
| dc.description.abstract | 在本實驗中，我們利用重組蛋白和親和管柱進行細胞外(in vitro)的實驗，發現重組syndecan-2和RACK1有交互作用，而在細胞內(in vivo)實驗，經免疫沉澱法和細胞免疫染色法也證實RACK1的確存在於syndecan-2蛋白複合體中，且兩分子的交互作用主要位於細胞膜上。進一步探討syndecan-2和RACK1的作用，我們發現當syndecan-2第180位置的酪胺酸在磷酸化狀態下，和RACK1的交互作用有增強的現象。
另一方面，我們以已知會造成syndecan-2酪氨酸磷酸化的細胞素(cytokine)，巨噬細胞群叢形成促進因子(GM-CSF)處理細胞，syndecan-2和RACK1的作用不論是有無磷酸化的參與(phosphate-dependent or independent)皆有增強的現象，且有時間和劑量上的效應(time-and dose-dependent effect)，該現象推測可能是與syndecan-2的磷酸化及syndecan-2的叢聚現象(clustering)所造成，但仍需更進一步實驗證實。 最後，我們試著分析syndecan-2在細胞生理的功能，並探討與RACK1交互作用的相關性，來進一步了解這兩個分子交互作用可能的細胞生理意義。由上述實驗結果，我們知道syndecan-2胞內區域磷酸化會增加兩分子間的作用，因此我們建構了正常型(wild type：Syn-2-wt)、酪胺酸單點突變(Syn-2-Y180F、Syn-2-Y192F、Syn-2-Y180/192F)及胞內區域(intracellular domain：PEP Syn-2-cyto) 的syndecan-2表現載體，在細胞中進行表現後，分別以細胞型態、貼附、爬移和增生等生理現象和生化方式進行觀察和分析，我們發現在不同載體表現的轉染細胞中，其細胞生理現象的差異和syndecan-2 /RACK1磷酸化參與交互作用的部分具有關聯性，然而是否藉由該作用的差異性來調控可能的分子(如：Src kinase or Protein kinase c)進而造成細胞生理現象的差異，其中的分子機轉仍須進一步的實驗證明。在本實驗中雖然無法明確了解syndecan-2和RACK1交互作用的意義，但仍提供了將來可能的實驗方向。 | zh_TW |
| dc.description.abstract | In this study, RACK1 (Receptor for Activated C Kinase 1) was found to be reactive with syndecan-2 in vitro and in vivo. Through affinity column chromatography and immunoprecipitation analysis as well as immunocytochemical colocalization studies, the reaction between RACK1 and syndecan-2 was evidenced in BALB/3T3 cells. Recombinant syndecan-2 and PEP Syn-2-cyto were applied to demonstrate that tyrosine 180 of syndecan-2 is a targeted site for Src tyrosine kinase and the reaction with RACK1 is enhanced after this tyrosine phosphorylation. In parallel, when granulocyte-macrophage colony-stimulating factor (GM-CSF) was applied to activate cellular tyrosine kinase of HeLa cells, a significant positive interaction was revealed between syndecan-2 and RACK1 with time and dose-dependence.
HeLa cells were further subject to transfections with wild type and mutant syndecan-2 vectors(Syn-2-Y180F、Syn-2-Y192F、Syn-2-Y180/192F) to show that the reaction of syndecan-2 with RACK1 was suppressed when tyrosine 180 phosphorylation site was absent. To elucidate the physiological significance of this selective reaction between syndecan-2 and RACK1, studies with adhesion, migration, and poliferation were focused. HeLa cells transfected with Syn-2-Y180F mutant vectors exhibited less motile and adhesion ability. Furthermore, the morphology of Syn-2-Y180F transfants became round and refractile. These results imply that tyrosine 180 of syndecan-2 may involve in the cytoskeleton organization, focal contacts formation, and tyrosine kinase regulation through selective reaction with RACK1. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T04:23:20Z (GMT). No. of bitstreams: 1 ntu-95-R93b41029-1.pdf: 6948657 bytes, checksum: 2ff76b05371e4d45e3a72df6ba5e83f6 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 中文摘要..................................................1
ABSTRACT..................................................2 壹、前言 1.Syndecans 家族的特性...................................3 2.Syndecan-2的基本特性....................................4 3.RACK1的基本特性.........................................6 4.實驗動機................................................7 5.實驗架構................................................7 貳、器材與藥品 1. 生物材料...............................................8 2. 實驗材料...............................................8 3. 實驗設備...............................................8 4. 實驗藥品與試劑.........................................9 5. 引子的設計............................................14 參、實驗方法 抽取總量RNA..............................................15 置備cDNA.................................................15 聚合酶連鎖反應...........................................16 DNA 電泳和EtBr染色.......................................16 純化DNA產物..............................................17 syndecan-2 cDNA引子的設計與全長和胞內功能區域DNA片段的獲得.......................................................17 質體的構築...............................................18 構築小鼠全長與胞內功能區域syndecan-2 cDNA在細菌表現質體的 保存與定序...............................................19 小鼠全長與胞內功能區域syndecan-2 cDNA在細菌表現質體的構築19 構築全長與胞內功能區域syndecan-2 cDNA在哺乳動物細胞表現質 體的保存與序.............................................20 全長與胞內功能區域syndecan-2 cDNA在哺乳動物細胞表現質體的 構築.....................................................21 單鹼基點突變小鼠syndecan-2 cDNA 在細菌和哺乳動物細胞表現 質體的構築...............................................21 抽取小量質體.............................................23 細菌重組蛋白質的誘導表現.................................23 全長、胞內功能區域syndecan-2 和RACK1重組蛋白的分離與純化24 蛋白質定量...............................................25 Tricine-硫酸十二酯鈉聚丙烯醯氯膠電泳分析.................25 Coomassie blue R-250 染色法..............................26 西方點墨分析法...........................................26 細胞培養基...............................................26 細胞培養.................................................27 全長syndecan-2及其點突變株的細胞轉染.....................27 篩選轉染有全長syndecan-2及其點突變株的細胞株群...........28 細胞免疫染色.............................................28 細胞膜的粗萃.............................................28 免疫沉澱法...............................................29 細胞進行粒細胞/巨噬細胞群叢形成促進因子的處理............29 Hitrap-PEP taxol 和syndecan-2親和管柱的製備與操作........30 Hitrap- syndecan-2-PEP cyto 或RACK1 鎳離子嵌合親和管柱的 製備與作.................................................31 重組Src (p60c-src) 對syndecan-2、syndecan-2-Y180F syndecan-2-PEP cyto的磷酸化作用..........................31 細胞增生分析.............................................32 細胞貼附分析.............................................32 細胞遷移能力分析.........................................33 肆、結果 全長和胞內syndecan-2 cDNA 的選殖以及點突變的建構和定序...34 小鼠全長和胞內syndecan-2重組蛋白的表現和純化.............34 以親和管柱色層分析證實重組蛋白RACK1和全長及胞內syndecan-2 具有交互作用的現象.......................................35 以親和管柱色層分析細胞中RACK1和syndecan-2-PEPCYTO重組蛋白 的交互作用，和免疫沉澱法證實syndecan-2和RACK1 complex 在 細胞中的存在.............................................36 以共軛焦顯微鏡觀察syndecan-2和RACK1在細胞中的重疊位置....36 syndecan-2-PEP cyto、syndecan-2和syndecan-2-Y180 F進行Src（p60 c-src）的磷酸化作用................................37 syndecan-2-PEP cyto在Src（p60 c-src）的磷酸化作用下有增強 與RACK1交互作用的現象....................................37 HeLa細胞在GM-CSF處理下，syndecan-2和RACK1交互作用有增強的 現象.....................................................38 HeLa細胞轉染syndecan-2基因作表現，觀察細胞型態...........39 HeLa細胞轉染syndecan-2及其突變株作基因表現後，分別進行細 胞貼附分析...............................................39 HeLa細胞轉染syndecan-2的突變株作基因表現後，觀察細胞型態 的差異...................................................40 HeLa細胞轉染syndecan-2及其突變株作基因表現後，分別進行細 胞遷移能力試驗...........................................41 HeLa細胞轉染syndecan-2及其突變株作基因表現後，進行細胞增 生分析...................................................42 HeLa細胞轉染syndecan-2胞內區域，觀察細胞型態的差異.......43 HeLa細胞轉染syndecan-2及其突變株作基因表現後，粗萃細胞膜 後分別進行免疫沉澱法.....................................43 伍、討論.................................................45 陸、參考文獻.............................................53 柒、圖表.................................................60 | |
| dc.language.iso | zh-TW | |
| dc.title | 探討syndecan-2和RACK1的交互作用與其細胞生理意義之研究 | zh_TW |
| dc.title | The functional role of syndecan-2 in the molecular interaction with RACK1 in cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 齊肖琪 | |
| dc.contributor.oralexamcommittee | 潘建源,黃偉邦 | |
| dc.subject.keyword | Syndecan-2,RACK1, | zh_TW |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-23 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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