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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝銘鈞 | |
dc.contributor.author | Tzu-Yu Lai | en |
dc.contributor.author | 賴子瑜 | zh_TW |
dc.date.accessioned | 2021-06-13T04:15:20Z | - |
dc.date.available | 2008-07-27 | |
dc.date.copyright | 2006-07-27 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-24 | |
dc.identifier.citation | REFERENCES
1 S.K. Pushpan†, S. Venkatraman†, V.G. Anand†, J. Sankar†, D. Parmeswaran#, S. Ganesan# and T.K. Chandrashekar †* Curr. Med Chem Anti-Cancer Agents, 2002, 2, 187-207. 2 Kennedy J. C., Pottier R. H., Pross D. C., J. Photochem. Photobiol. B Biol., 1990, 6, 143-148. 3 Spikes D. J., Ann. N. Y. Acad. Sci., 1975, 244, 496-508. 4 Ochsner M., J. Photochem. Photobiol. B Biol., 1997, 39, 1-18. 5 Philips D., Prog. Reaction Kinetics, 1997, 22, 175-300. 6 T.J. Dougherty, C.J. Gomer, B.W. Henderson, G. Jori, D. Kessel, M. Korbelik, J. Moan, Q. Peng, Photodynamic therapy,J. Natl. Cancer Inst., 1998, 90, 889–905. 7 R.A. Hsi, D.I. Rosenthal, E. Glatstein, Photodynamic therapy in the treatment of cancer, Drugs, 1999,57, 725–734. 8 N.L. Oleinick, H.H. Evans, The photobiology of photodynamic therapy: cellular targets and mechanisms, Radiation Res., 150 (1998) S146–156. 9 Kessel, D., & Luo, Y. (1998). Mitochondrial photodamage and PDT induced apoptosis. Journal of Photochemistry and Photobiology B: Biology, 42, 89–95. 10 Morgan, J., & Oseroff, A. R. (2001). Mitochondria-based photodynamic anti-cancer therapy. Advanced Drug Delivery Reviews, 49, 71–86. 11 Oleinick, N. L., Morris, R. L., & Belichenko, I. (2002). The role of apoptosis in response to photodynamic therapy: What, where, why and how. Photochemical and Photobiological Sciences, 1, 1–21. 12 WANG, X. The expanding role of mitochondria in apoptosis. Genes Dev. 15: 2922–2933 2001. 13 David Kessel*, Raymond Luguya and M. Graca H. Vicente Photochemistry and Photobiology, 2003, 78(5): 431–435. 14 Adler, A. D.; Longo, F. R.; Finarelli, J. D.; Goldmacher, J.; Assour, J.; Korsakoff, L. J. Org. Chem., 1967, 32, 476. 15 Leo, A., Hansch, C., & Elkins, D. (1971). Partition coefficients and their uses. Chemical Reviews, 71, 525–616. 16 Noemı´ Rubio, David Sa´nchez-Garcı´a, Ana Jime´nez-Banzo, OÄ scar Rey, Jose´ I. Borrell, Jordi Teixido´, and Santi Nonell* J. Phys. Chem. A 2006, 110, 3480-3487 17 Ludovic Bourre´, Sonia Thibaut, Ame´lie Briffaud, Nathalie Rousset, Sabine Ele´ouet, Youenn Lajat, Thierry Patrice* Journal of Photochemistry and Photobiology B: Biology 67 (2002) 23–31. 18 Marie-Hele`ne Teiten, Sophie Marchal, M. A. D’Hallewin, Franc﹐ois Guillemin and Lina Bezdetnaya* Photochemistry and Photobiology, 2003, 78(1): 9–14. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32776 | - |
dc.description.abstract | 近年來,有關於研發更有效率的光感藥物應用在光動力治療(PDT)上,偏向針對於光感藥物的物化性質探討,像是吸收更往紅光區位移或是singlet oxygen的產生效率以及控制光感藥物在腫瘤細胞內分佈的位置 。根據它們的物化性質和細胞攝取機制,光感藥物能達到不同的細胞內給藥濃度和細胞內分佈位置。而且,若光感藥物能選擇性地分佈在目標胞器 ,例如線粒體或高基氏體 ,就能夠決定細胞的死亡路徑。
在我的研究中發現到,光感藥物的結構與它的傳遞機制和細胞攝取有緊密的正相關。而且經過ROS的偵測,可以發現隨著給藥時間的增加,細胞攝取也增加而且更多的ROS也隨之被偵測到。 | zh_TW |
dc.description.abstract | The search for new efficient photosensitizers in photodynamic therapy (PDT) points to improve photophysical properties like absorption in the red region and singlet oxygen quantum yield as well as to control the localization of the sensitizer within the tumor cell. Depending on their physicochemical properties and their uptake mechanism, photosensitizers can reach different intracellular concentrations and localize in different subcellular compartments . Moreover, the preferential localization of a photosensitizer in target organelles, such as mitochondria or Golgi apparatus, can determine the cell death mechanism after PDT.
The intracellular uptake was found to be closely related to the molecular structure of sensitizers and its subcellular trafficking in our study. Furthermore, Our result also revealed that more ROS were detected according to the increased incubation time which resulted in more cellular uptake of photosesitizers . | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:15:20Z (GMT). No. of bitstreams: 1 ntu-95-R93548051-1.pdf: 1206935 bytes, checksum: aef2db230f96284fa8b27c5d90b8de62 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | CONTENT
中文摘要…………………………………………………………………1 ABSTRACT……………………………………………………………..2 INTRODUCTION………………………………………………………3 MATERIALS and METHODS………………………………………...5 The framework of the experiments………………………………….…5 Synthesis of meso-substituted porphyrins……………………………..6 Octanol–Water Partition Coefficients……………………………….…6 The quantum yield of singlet oxygen production……………………...7 Singlet Oxygen detection……………………………………………...8 Cell culture and Incubation conditions………………………………...8 Intracellular uptake………………………………………………….…9 Cytotoxicity and PDT effect study………………………………….…9 ROS Detection………………………………………………………..10 Intracellular localization by confocal laser scanning microscopy……10 RESULTS and DISCUSSION…...………………………….………...14 Synthesis and Characterization of meso-substituted porphyrins……..14 Octanol–Water Partition Coefficients………………………………...14 The quantum yield of singlet oxygen production….…………………14 Singlet Oxygen detection…………………………………………….14 Intracellular uptake…………………………………………………...17 Cytotoxicity and PDT effect study…………………………………...17 ROS Detection………………………………………………………..18 Intracellular Localization……………………………………………..19 CONCLUSION…….……………………………………………...…...36 REFERENCES………………………………………….……………..38 TABLES Table 1. Photophysical properties of meso-substituted porphyrins……..16 FIGURES FIG 1 Synthesis of meso-substituted porphyrins………………………12 FIG 2 The structural formulas of all compounds……………………...13 FIG 3 NMR spectrum of all porphyrins………………………………..20 FIG 4 Absorption spectrum……………………………………………27 FIG 5 Near-IR phosphorescence spectrum of porphyrins……………..27 FIG 6 Singlet Oxygen detection……………………………………….28 FIG 7-8 Dose-dependence of cellular uptake………………………….29 FIG 9 Intracellular uptake with different incubation time……………..30 FIG 10 Hela cells treated with different illumination time without porphyrins……………………………………………30 FIG 11 Cytotoxicity in the drug dose-response……………………..…31 FIG 12-13 Cytotoxicity in the drug dose-response and then irradiated with blue light (λmax =435nm) for 40s………31 FIG 14-15 Light dose-response PDT effect…………………………...32 FIG 16-17 PDT effect with different incubation time…………………33 FIG 18 ROS detection in the dark condition…………………………..34 FIG 19 ROS detection in the light condition…………………………..34 FIG 20 Confocal fluorescence images of Hela cells double stained with porphyrins and organelle probes………………..35 | |
dc.language.iso | en | |
dc.title | 探討meso-substituted porphyrins上不同的
取代基對於細胞攝取、細胞內分佈位置 以及光動力治療效果的影響 | zh_TW |
dc.title | Influence of substitutions on meso-substituted porphyrins with regard to intracellular uptake , subcellular localization and photodynamic therapy (PDT) effect | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 婁培人,王先知,張健忠,楊台鴻 | |
dc.subject.keyword | 光感藥物,光動力治療,細胞攝取,ROS偵測, | zh_TW |
dc.subject.keyword | photosensitizers,photodynamic therapy,Intracellular uptake,ROS detection, | en |
dc.relation.page | 39 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-25 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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