請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32736
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 莊榮輝(Rong-Huay Juang) | |
dc.contributor.author | Yu-Jen Wu | en |
dc.contributor.author | 吳裕仁 | zh_TW |
dc.date.accessioned | 2021-06-13T04:14:28Z | - |
dc.date.available | 2006-07-28 | |
dc.date.copyright | 2006-07-28 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-25 | |
dc.identifier.citation | 林維治 (1994) 林維治先生竹類論文集。林業叢刊第 69 號,張添榮主編,台灣省林
業試驗所 莊榮輝 (1988) 水稻蔗糖合成脢之研究 國立台灣大學農業化學研究所 博士論文 陳翰民 (1997) 甘藷塊根澱粉磷解脢構造與功能之研究 國立台灣大學農業化學研究 所 博士論文 張世宗 (1999) 甘藷塊根Chaperonin 及 Proteosome 之分離與性質研究 國立台灣 大學農業化學研究所 博士論文 林珮君 (2000) 甘藷塊根澱粉磷解脢在澱粉代謝之探討 國立台灣大學農業化學研 究所 碩士論文 蔡承佳 (2003) 蛋白質磷酸化對水稻蔗糖合成脢酵素功能及基因表現的影響 國立台 灣大學農業化學研究所 博士論文 楊光華 (2005) 甘藷塊根澱粉磷解脢激脢之純化與性質分析 國立台灣大學微生物 與生化研究所 博士論文 Amor Y, Haigler CH, Johnson S, Wainscott M, Delmer DP (1995) A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc Natl Acad Sci 92: 9353-9357 Anderson LE , Ringenberg MR, Brown VK and Carol AA. (2005) Both chloroplastic and cytosolic phosphofructoaldolase isozymes are present in the pea leaf nucleus. Protoplasma. 225(3-4):235-42. Anderson LE, Wang X, Gibbons JF (1995) Three enzymes of carvbon metabolism. Or their antigenic analogs, in pea nuclei. Plant Physiol 108:659-667 Arenkov P, Kukhtin A, Gemmell A, Voloshchuk S, Chupeeva V, Mirzabekov A. (2000) Protein microchips: use for immunoassay and enzymatic reactions. Anal Biochem. 278(2):123-31. Assmann SM (2002) Heterotrimeric and unconventional GTP binding proteins in plant signalling. Plant Cell 14 (Suppl.): S355–S373 Babiychuk E, Kushnir S, Belles-Boix E, Van Montagu M. and Inzé D. (1995) Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamine, J. Biol. Chem. 270 26224–262 Batorfi J, Ye B, Mok SC, Cseh I, Berkowitz RS, Fulop V. (2003) Protein profiling of complete mole and normal placenta using ProteinChip analysis on laser capture microdissected cells. Gynecol Oncol. 88 (3):424-8. Bhalerao R, Uhlen M, Teeri TT, Lundeberg J, Sundberg B, Nilsson P, Sandberg G. (2001) A transcriptional roadmap to wood formation. Proc Natl Acad Sci 98(25):14732-7. Bayer E, Thomas CL, Maule AJ. (2004) Plasmodesmata in Arabidopsis thaliana suspension cells. Protoplasma. 223(2-4):93-102. Epub 2004 Jun 22. Becker M, Vincent C, Reid JSG (1995) Biosynthesis of (1, 3)(1, 4)-β-glucan and (1, 3)- β-glucan in barley (Hordeum vulgare L.): properties of the membrane-bound glucan synthases. Planta 195: 331-338 Borisjuk N, Sitailo L, Adler K, Malysheva L, Tewes A, Borisjuk L, Manteuffel R (1998) Calreticulin expression in plant cells: developmental regulation, tissue specificity and intracellular distribution. Planta 206: 504–514 Botha FC, Dennis DT. (1986). Phosphoglyceromutase activity and concentration in the endosperm of developing and germinating Ricinus communis seeds. Canadian Journal of Botany 65, 1908–1912 Campbell RE, Brett CT, Hillman JR (1988) A xylosyltransferase involved in the synthesis of a protein-associated xyloglucan in suspension-cultured dwarf-French-bean (Phaseolus vulgaris) cells and its interaction with a glucosyltransferase. Biochem J. Aug 253(3):795-800. Casacuberta JM, Raventos D, Puigdomenech P, San Segundo B. (1992) Expression of the gene encoding the PR-like protein PRms in germinating maize embryos. Mol Gen Genet. 234(1):97-104. Ciereszko I, Johansson H, Kleczkowski LA. (2001) Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis. Biochem J. 354(Pt 1):67-72. Choudhary AD, Lamb CJ, Dixon RA. (1990) Stress Responses in Alfalfa (Medicago sativa L.): VI. Differential Responsiveness of Chalcone Synthase Induction to Fungal Elicitor or Glutathione in Electroporated Protoplasts. Plant Physiol. 94(4):1802-1807. Coleman H, Ellis D, Gilbert M, Mansfield SD (2003) Increased growth and yield by altered carbohydrate allocation . In B Sundberg, ed, Tree Biotechnology UPSC Publixhers, Umea, Sweden, ppS10.41 Cotelle V, Meek SEM, Provan F, Milne FC, Morrice N, MacKintosh C. (2000). 14-3-3s regulate global cleavage of their diverse binding partners in sugar-starved Arabidopsis cells. EMBO Journal 19, 2869–2876. Comparot S, Lingiah G, Martin T. (2003) Function and specificity of 14-3-3 proteins in the regulation of carbohydrate and nitrogen metabolism. J Exp Bot. 54(382):595-604 Coppolino MG, Woodside MJ, Demaurex N, Grinstein S, St-Arnaud R, Dedhar S. (1997) Calreticulin is essential for integrin-mediated calcium signalling and cell adhesion. Nature. 386(6627):843-7. Cronshaw JM, Krutchinsky AN, Zhang W, Chait BT, Matunis MJ. (2002) Proteomic analysis of the mammalian nuclear pore complex. J Cell Biol. 158(5):915-27. Davies EJ, Tetlow IJ, Bowsher CG, Emes MJ.(2003) Molecular and biochemical characterization of cytosolic phosphoglucomutase in wheat endosperm (Triticum aestivum L. cv. Axona). J Exp Bot. 54(386):1351-60 Denecke et al., J. Denecke, R. De Rycke and J. Botterman. (1992) Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope, EMBO J. 11. 2345–2355. Denecke J, Carlsson LE, Vidal S, Hoglund AS, Ek B, van Zeijl MJ, Sinjorgo KM, Palva ET (1995) The tobacco homolog of mammalian calreticulin is present in protein complexes in vivo. Plant Cell 7: 391–406 Dhugga KS, Tiwari SC, Ray PM. (1997) A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: purification, gene cloning, and trans-Golgi localization. Proc Natl Acad Sci. 94(14):7679-84. Dixon RA, Paiva NL (1995) Stress-Induced Phenylpropanoid Metabolism.Plant Cell. 7(7):1085-1097. Dominguez-Solis JR, Gutierrez-Alcala G, Vega JM, Romero LC, Gotor C. (2001) The cytosolic O-acetylserine(thiol)lyase gene is regulated by heavy metals and can function in cadmium tolerance. J Biol Chem.;276(12):9297-302. Drincovich MF, Casati P, Andreo CS. (2001) NADP-malic enzyme from plants: a ubiquitous enzyme involved in different metabolic pathways. FEBS Lett. 490(1-2):1-6. Edwards GE, Andreo CS. (1992) NADP-malic enzyme from plants.Phytochemistry. 31(6):1845-57 Emili AQ, Cagney G. .(2000) Large-scale functional analysis using peptide or protein arrays. Nat Biotechnol . 18(4):393-7 Faïk A, Chileshe C, Sterling J, and Maclachlan G (1997) Xyloglucan galactosyl- and fucosyltransferase activities from pea epicotyl microsomes. Plant Physiol. 114(1): 245–254. Ferl RJ, Lu GH, Bowen BW. (1994). Evolutionary implications of the family of 14-3-3 brain protein homologs in Arabidopsis thaliana. Genetica. 92, 129–138 Ferrari and Soling, D.M. Ferrari and H.D. Soling, (1999) The protein disulphide-isomerase family: unravelling a string of folds, Biochem. J. 339. 1–10. Fernie AR, Tauberger E, Lytovchenko A, Roessner U, Willmitzer L, Trethewey RN. (2002) Antisense repression of cytosolic phosphoglucomutase in potato (Solanum tuberosum) results in severe growth retardation, reduction in tuber number and altered carbon metabolism. Planta. 214(4):510-20. Forrer P, Stumpp MT, Binz HK, Pluckthun A (2003) A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett. 539(1-3):2-6. Franke KE, Adams DO (1995) Cloning of a full-length cDNA for malic enzyme (EC 1.1.1.40) from grape berries. Plant Physiol 107: 1009-1010 Freedman et al., R.B. Freedman, T.R. Hirst and M.F. Tuite (1994) Protein disulphide isomerase: building ridges in protein folding, Trends Biochem. Sci. 19 . 331–336 Fujisawa Y, Kato H, Iwasaki Y (2001) Structure and function of heterotrimeric G proteins in plants. Plant Cell Physiol 42: 789–794 Fukui T (1983) Plant phosphorylase:structure and function. In T Ajazawa, T Asahi, He Imaseki, eds, The New Frontiers in Plant Biochemistry, Ed Japan Sceintific Societies Press Tokyo, pp 71-82 Gerstein M, Lan N, Jansen R. (2002) Proteomics. Integrating interactomes. Science. 295(5553):284-7. German MA, Dai N, Matsevitz T, Hanael R, Petreikov M, Bernstein N, Ioffe M, Gibeaut DM (2000) Nucleotide sugars and glucosyltransferases for slynthesis of cell wall matrix polysaccharides. Plant Physiol Biochem 38: 69-80 Gygi SP, Rochon Y, Franza BR, Aebersold R. (1999) Correlation between protein and mRNA abundance in yeast. Mol Cell Biol. 19(3):1720-30. Haab BB, Dunham MJ, Brown PO. (2001) Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions.Genome Biol. 2(2):1-13 Haigler CH, Ivanova-Datcheva M, Hogan PS, Salnikov VV, Hwang S, Martin K, Delmer DP. (2001) Carbon partitioning to cellulose synthesis. Plant Mol Biol. 47(1-2):29-51. Hallborn J, Carlsson R. (2002) Automated screening procedure for high-throughput generation of antibody fragments. Biotechniques Suppl:30-7 Hanftey C, Fife M, Buchanan-Wollaston V. (1996) Leaf senescence in Brassica napus: expression of genes encoding pathogenesis-related proteins. Plant Molecular biology 30:597-609 Hendrick JP, Hartl FU. (1993) Molecular chaperone functions of heat-shock proteins. Annu Rev Biochem.62:349-84. Hansen, K.M., Thuesen.A.B. and Soderberg. J.R. (2001) Enzyme assay for identification of pectin derivatives, based on recombinant pectate lyase. J.AOAC International, 84, 1851-1854 Hassid, W.Z. (1962) The biosynthesis of polysaccharides from mucleoside diphosphate sugers. Biochem. Soc. Symp. 21, 79-93 Hayhurst A, Georgiou G (2001) High-throughput antibody isolation. Curr Opin Chem Biol. 5(6):683-9. Henmi K, Tsuboi S, Demura T, Fukuda H, Iwabuchi M, Ogawa KI (2001) A possible role of glutathione and glutathione disulfide in tracheary element differentiation in the cultured mesophyll cells of Zinnia elegans. Plant Cell Physiol.;42(6):673-6. Hertzberg M, Aspeborg H, Schrader J, Andersson A, Erlandsson R, Blomqvist K, Bhalerao R, Uhlen M, Teeri TT, Lundeberg J, Sundberg B, Nilsson P, Sandberg G. (2001) A transcriptional roadmap to wood formation. Proc Natl Acad Sci. 98(25):14732-7. Hertzberg M, Aspeborg H, Schrader J, Andersson A, Erlandsson R, Blomqvist K, Johnsson B, Lofas S, Lindquist G. (1991) Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. Anal Biochem. 198(2):268-77. Holt LJ, Bussow K, Walter G, Tomlinson IM. (2000) By-passing selection: direct screening for antibody-antigen interactions using protein arrays. Nucleic Acids 28(15):E72 Huang Y, Blakeley SD, McAleese SM, Fothergill-Gillmore LA, Dennis DT. (1993) Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression. Plant Molecular Biology 23, 1039–1053 Huber SC, MacKintosh C, Kaiser WM (2002) Metabolic enzymes as targets for 14-3-3 proteins. Plant Mol Biol. 50(6):1053-63. Ito H, Iwabuchi M, Ogawa K. (2003) The sugar-metabolic enzymes aldolase and triose-phosphate isomerase are targets of glutathionylation in Arabidopsis thaliana: detection using biotinylated glutathione. Plant Cell Physiol. 44(7):655-60 Jaubert S, Ledger TN, Laffaire JB, Piotte C, Abad P, Rosso MN (2002) Direct identification of stylet secreted proteins from root-knot nematodes by a proteomic approach. Mol Biochem Parasitol 121: 205–211 Jedrzejas MJ. (2000) Structure, function, and evolution of phosphoglycerate mutases: comparison with fructose-2,6,-bisphosphatase, acid phosphatase, and alkaline phosphatase. Progress in Biophysics and Molecular Biology 73, 263–287. Jones AM (2002) G-protein-coupled signalling in Arabidopsis. Curr Opin Plant Biol 5: 402–407 Jones AM, Ecker JR, Chen J-G (2003) A re-evaluation of the role of the heterotrimeric G protein in coupling light responses in Arabidopsis. Plant Physiol 131: 1623–1627 Jones VW, Kenseth JR, Porter MD, Mosher CL, Henderson E. (1998) Microminiaturized immunoassays using atomic force microscopy and compositionally patterned antigen arrays. Anal Chem. 70(7):1233-41. Kimura S, Kondo (2002) Recent progress in cellulose biosynthesi. J Plant Res 115:297-302 Kleczkowski LA, Geisler M, Ciereszko I, Johansson H. (2004) UDP-glucose pyrophosphorylase. An old protein with new tricks. Plant Physiol. 134(3):912-8. Konishi H, Yamane H, Maeshima M, Komatsu S. (2005) Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling. Plant Mol Biol. 56(6):839-48. Krömer S (1995) Respiration during photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 46: 45-70 Lers A, Burd S, Lomaniec E, Droby S. and Chalutz E. (1998) The expression of a grapefruit gene encoding an isoflavone reductase-like protein is induced in response to UV irradiation, Plant Mol. Biol. 36, 847–856. Liberek K, Georgopoulos C. (1993) Autoregulation of the Escherichia coli heat shock response by the DnaK and DnaJ heat shock proteins. Proc Natl Acad Sci 90(23):11019-23. Liu CJ, Dixon RA. (2001)Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis. Plant Cell. 13(12):2643-58. Lu M, Sautin YY, Holliday LS, Gluck SL. (2004) The glycolytic enzyme aldolase mediates assembly, expression, and activity of vacuolar H+-ATPase. J Biol Chem. 279(10):8732-9 Lucero and Kaminer, H.A. Lucero and B. Kaminer. (1999) The role of calcium on the activity of ER calcistorin/Protein disulfide isomerase and the significance of the C-terminal and its calcium binding. A comparison with mammalian protein-disulfide isomerase, J. Biol. Chem. 274. 3243–3251. MacBeath G, Schreiber SL.(2000) Printing proteins as microarrays for high-throughput function determination. Science. 289(5485):1760-3. MacBeath G. (2001) Proteomics comes to the surface. Nat Biotechnol. 19(9):828-9 Mackenzie S, McIntosh L. (1999) Higher plant mitochondria Plant Cell. 11(4):571-581 Ma H (1994) GTP-binding proteins in plants: new members of an old family. Plant Mol Biol 26: 1611–1636 Mang HG, Kang EO, Shim JH, Kim SY, Park KY, Kim YS, Bahk YY, Kim WT. (2004) A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings. Biochim Biophys Acta. 1676(3):231-9. Marrs KA. (1996) The function s nad regulation of glutathione S-transferases in plants Annu Rev Plant Physiol Plant Mol Biol. 47:127-158. Marrs KA, Casey ES, Capitant SA, Bouchard RA, Dietrich PS, Mettler IJ, Sinibaldi RM. (1993) Characterization of two maize HSP90 heat shock protein genes: expression during heat shock, embryogenesis, and pollen development. Dev Genet. 14(1):27-41. Matic S, Akerlund HE, Everitt E, Widell S. (2004) Sucrose synthase isoforms in cultured tobacco cells. Plant Physiol Biochem. 42(4):299-306. McCarthy RM, Farmer P, Sheehan D. (1996) Binding of 2-hydroxy-5-nitrobenzyl alcohol to rat alpha class glutathione S-transferases; evidence for binding at tryptophan 21. Biochim Biophys Acta. 1293(2):185-90 McCafferty j, Griffiths A. D, Winter G, Chiswell D. J. (1990) Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348:552-554. McCann MC, Stacey NJ, Wilson R, Roberts K. (1993) Orientation of macromolecules in the walls of elongating carrot cells. J Cell Sci. (4):1347-56. Merrick, W.C. and Nyborg, J.(2000) In translational control of gene expressinon (Sonenberg, N., Hershey, J.W.B and Mathew, MB.,eds) Cold Spring Habor laboratouy press. 89-126 Moffatt BA, Stevens YY, Allen MS, Snider JD, Pereira LA, Todorova MI, Summers PS, Weretilnyk EA, Martin-McCaffrey L, Wagner C. (2002) Adenosine kinase deficiency is associated with developmental abnormalities and reduced transmethylation. Plant Physiol 128(3):812-21 Neuburger M, Day DA, Douce R.(1985) Transport of NAD in Percoll-Purified Potato Tuber Mitochondria: Inhibition of NAD Influx and Efflux by N-4-Azido-2-nitrophenyl-4-aminobutyryl-3'-NAD. Plant Physiol. 78(2):405-410. Neuburger, M., Rebeille, F., Jourdain, A., Nakamura, S., and Douce, R. (1996) Mitochondria are a major site for folate and thymidylate synthesis in plants. J. Biol. Chem. 271(16):9466-72. Nolte KD, Koch KE. (1993) Companion-Cell Specific Localization of Sucrose Synthase in Zones of Phloem Loading and Unloading. Plant Physiol. 101(3):899-905. O'Neill MA, Ishii T, Albersheim P, Darvill AG. (2004) Rhamnogalacturonan II: structure and function of a borate cross-linked cell wall pectic polysaccharide. Annu Rev Plant Biol. 55:109-39. Okamoto H, Matsui M, Deng XW (2001) Overexpression of the heterotrimeric G-protein αsubunit enhances phytochrome-mediated inhibition of hypocotyl elongation. Plant Cell 13: 1639–1652 Palomo J, Gallardo F, Suarez MF, Canovas FM. (1998) Purification and characterization of NADP+-linked isocitrate dehydrogenase from scots pine . Evidence for different physiological roles of the enzyme in primary development Plant Physiol. 118(2):617-26 Pandey A, Mann M. (2000) Proteomics to study genes and genomes. Nature. 405(6788):837-46. Read SM, Bacic T. (2002) Plant biology. Prime time for cellulose. Science. 295(5552):59-60. Peng L, Kawagoe Y, Hogan P, Delmer D. (2002) Sitosterol-beta-glucoside as primer for cellulose synthesis in plants. Science. 295(5552):147-50. Petrucco S, Bolchi A, Foroni C, Percudani R, Rossi G.L. and Ottonello S., A maize gene encoding an NADPH binding enzyme highly homologous to isoflavone reductases is activated in response to sulfur starvation, Plant Cell 8 (1996), pp. 69–80 Persson S, Rosenquist M, Svensson K, Galvao R, Boss WF, Sommarin M (2003) Phylogenetic analyses and expression studies reveal two distinct groups of calreticulin isoforms in higher plants. Plant Physiol. 133(3):1385-96. Persson S, Wyatt SE, Love J, Thompson WF, Robertson D, Boss WF (2001) The Ca2+ status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants. Plant Physiol 126: 1092–1104 Raghavendra AS, Padmasree K, Saradadevi K (1994) Interdependence of photosynthesis and respiration in plant cells: interactions between chloroplast and mitochondria. Plant Sci 97: 1-14 Rommens CM, Salmeron JM, Oldroyd GE, Staskawicz BJ (1995) Intergeneric transfer and functional expression of the tomato disease resistance gene Pto. Plant Cell. 7(10):1537-44. Roberts MR, Bowles DJ. (1999) Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants. Plant Physiology 119, 1243–1250 Rose JK, Saladie M, Catala C. (2004) The plot thickens: New perspectives of primary cell wall modification. Curr Opin Plant Biol. 7(3):296-301. Poulton, J.E. & Butt, V.S. (1976) Purification and properties of S-adenosine- L-homocysteine hydrolase from leaves of spinach beet . Arch. Biochem. Biophys. 172, 135 142. Ray S, Anderson JM, Urmeev FI, Goodwin SB. (2003) Rapid induction of a protein disulfide isomerase and defense-related genes in wheat in response to the hemibiotrophic fungal pathogen Mycosphaerella graminicola. Plant Mol Biol. 53(5):701-14. Ruan YL, Llewellyn DJ, Furbank RT. (2003) Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell. 15(4):952-64. Sakai T, Sakamoto T, Hallaert J, Vandamme EJ. (1993) Pectin, pectinase and protopectinase: production, properties, and applications. Adv Appl Microbiol. 39:213-94. Saito K, Kurosawa M, Tatsuguchi K, Takagi Y, Murakoshi I.(1994) Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine(thiol)-lyase. Plant Physiol. 106(3):887-95. Schenk G, Duggleby RG, Nixon PF. (1998) Properties and functions of the thiamin diphosphate dependent enzyme transketolase. Int J Biochem Cell Biol. 30(12):1297-318 Singh RP, Setlow P. (1979) Purification and properties of phosphoglycerate phosphomutase from spores and cells of Bacillus megaterium. Journal of Bacteriology 137, 1024–1027. Shahak Y, Schaffer AA, Granot D. (2003) Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves. Plant J. 34(6):837-46. Shiue SM, Sing HY, Su TC. (1980) Purification and properties of uridine diphosphate glucose pyrophosphorylase from bamboo shoots. J. Chin. Bio. Soc. 9(1) Smith AP, Nourizadeh SD, Peer WA, Xu J, Bandyopadhyay A, Murphy AS, Goldsbrough PB. (2003) Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids. Plant J. 36(4):433-42. Su JC. (1965). Carbohydrate metabolism int the shoots of bamboo, Leleba oldhami. I. Preliminar survey of soulbe saccharides and sucrose-degrading enzymes. Bot. Bull. Acad. Sinica. 2, 153-159 Su JC, Chou IN, Tsai MJ. (1969) Carbohydrate metabolism in the shoot of bamboo Leleba oldhami VII. Changes of polysaccharide constituents accompanied with the growth of the plant. J. Chin. Agr. Chem Soc. Sp. Iss.:16-24 Su JC.(1969) Biosynthesis of cell wall polysaxxharides in higher plants:An annual progress report. 3,123-134 Su JC, TSou DS, Tai HH. (1967). Carbohydrate metabpolism in the shoots of bamboo, Leleba oldhami. IV. A structural study of cell wall polysaccharides. Bot. Bull. Acad. Sinica. 6, 153-159 Su JC, Wu JL, Yang CL. (1977) Purification and Characterization of sucrose synthetase from the of Bamboo Leleba oldhami. Plant physiol 60, 17-21. Su JC, Yuan HF, Sung HY.(1968). Carbohydrate metabolism in the shoots of Leleba oldhami. V.. The callose synthesizing system. J.Chin. Agr. Soc. Sp Iss:1-11 Sopychalla JP, Scheffler BE, Sowokinos JR, Bevan MW (1994) Cloning, antisense RNA inhibition and the coordinated expression of UDP-glucose pyrophosphorylase with starch biosynthetic genes in tpotato tubers. J Plant Physiol 144:444-453 Tetlow IJ, Wait R, Lu Z, Akkasaeng R, Bowsher CG, Esposito S, Kosar-Hashemi B, Morell MK, Emes MJ.(2004) Protein phosphorylation in amyloplasts regulates starch branching enzyme activity and protein-protein interactions. Plant Cell. 16(3):694-708 Thom R, Dixon DP, Edwards R, Cole DJ, Lapthorn AJ. (2001) The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism. J Mol Biol. 308(5):949-62 Van Eldik GJ, Wingens M, Ruiter RK, Van Herpen MM, Schrauwen JA, Wullems GJ. (1996) Molecular analysis of a pistil-specific gene expressed in the stigma and cortex of Solanum tuberosum. Plant Mol Biol. 30(1):171-6. Van der Straeten D, Rodrigues-Pousada RA, Goodman HM, Van Montagu M (1991) Plant enolase: gene structure, expression, and evolution. Plant Cell. 3(7):719-35 van Doorsselaere J, Villarroel R, van Montagu M, Inzé D (1991) Nucleotide sequence of a cDNA encoding malic enzyme from poplar. Plant Physio l 96: 1385-1386 Varns, J.L., and Sowokinos, J.R. (1974) Arapid micro-starch quantitation method for potato callus and its application with potato tubers. Am. Potato. J. 51, 383-392 Vernoux T, Wilson RC, Seeley KA, Reichheld JP, Muroy S, Brown S, Maughan SC, Cobbett CS, Van Montagu M, Inze D, May MJ, Sung ZR. (2000) The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell.12(1):97-110 Wang J-L, Walling LL, Jauh GY, GU Y-Q, Lord EM. (1996) Lily cofactor-independent phosphoglycerate mutase: purification, partial sequencing, and immunolocalization. Planta 200, 343–352 Wang X, Sirover MA, Anderson LE. (1999) Pea chloroplast glyceraldehyde-3-phosphate dehydrogenase has uracil glycosylase activity.15;367(2):348-53. Walter MH, Grima-Pettenati J, Grand C, Boudet AM, Lamb CJ (1990) Extensive sequence similarity of the bean CAD4 (cinnamyl-alcohol dehydrogenase) to a maize malic enzyme. Plant Mol Biol 15: 525-526 Weiler T, Sauder P, Cheng K, Ens W, Standing K, Wilkins JA. (2003) A proteomics-based approach for monoclonal antibody characterization. Anal Biochem. 321(2):217-25. Westram A, Lloyd JR, Roessner U, Riesmeier JW, Kossmann J. (2002) Increases of 3-phosphoglyceric acid in potato plants through antisense reduction of cytoplasmic phosphoglycerate mutase impairs photosynthesis and growth, but does not increase starch contents. Plant, Cell and Environment 25, 1133–1143. Wirtz M, Droux M. (2005) Synthesis of the sulfur amino acids: cysteine and methionine..Photosynth Res. 2005 86(3):345-62. Xenarios I, Eisenberg D. (2001) Protein interaction databases. Curr Opin Biotechnol. 12(4):334-9. Yun DJ, Zhao Y, Pardo JM, Narasimhan ML, Damsz B, Lee H, Abad LR, D'Urzo MP, Hasegawa PM, Bressan RA. (1997) Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein. Proc Natl Acad Sci . 94(13):7082-7. Zhao GR, Liu JY. (2002) Isolation of a cotton RGP gene: a homolog of reversibly glycosylated polypeptide highly expressed during fiber development. Biochim Biophys Acta 1574(3):370-4 Zhu H, Snyder M. (2001) Protein arrays and microarrays. Curr Opin Chem Biol. 5(1):40-5. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32736 | - |
dc.description.abstract | 針對竹筍快速生長的特性,本計畫利用『蛋白質體』工具,比對生長過程中蛋白質的總體變化。竹筍由未出土到生長六十公分的過程中,水溶性蛋白質具有消長的變化,目前鑑定及分析消長色點的身分,並且配合酵素活性分析,發現綠竹筍快速生長時,細胞壁多醣類快速轉變,糖解作用以及檸檬酸循環作用加速,以提供竹筍生長所需的能量;五碳醣磷酸路徑表現量保持恆定,持續提供核醣及NADPH,以提供綠竹生長時核酸、胺基酸及固醇類合成所需。另外一些參與生長的G-protein, elongation factor等蛋白質,以及防禦機制的蛋白質及代謝酵素表現量都增多,可能也是造成竹筍快速生長的原因。
另外以竹筍全體蛋白質進行單株抗體庫製備的研究,以全部的蛋白質進行小鼠免疫,再利用階段式抗體篩選方法,並且改變抗體製備及篩選流程,目前已經成功選出約192株不同單株抗體,此種方法是一全新的作法及概念,可以省去抗原的純化步驟,並且在短時間內製備出多樣化的單株抗體。而且結合蛋白質體及抗體庫的平台,是一個非常有用基礎技術平台,可對任何生物系統做相似的應用,產生任何蛋白質體的抗體庫 (小白鼠系統除外)。得到抗體庫後接著可進行抗體晶片的製作,以供相關生物系統在其細胞學、生理學、病理學等各範疇的基礎與應用研發。 | zh_TW |
dc.description.abstract | This project employing the proteomics method to investigate the overall changes of proteins during the growing process in bamboo which is fast-growing in nature. It was observed that the levels of water-soluble proteins fluctuate in the growing stage from underground to 60 cm-shoot high. Identifying the spots which undergo changes during the growing process in combination with enzyme activity analysis, it were discovered that polysaccharides of cell walls change rapidly, glycolysis and tricarboxylic acid cycle metabolism accelerate to generate the energy for growing, the level of pentose phosphate pathway remains stable to supply ribose and NADPH for the synthesis of nucleic acids, amino acids, and steroids. Additionally, the amounts of some proteins related to growing such as G-protein and elongation factor, proteins with defense mechanism and metabolism enzymes increase to cause the fast-growing of bamboo.
In a separate research, the establishment of monoclonal antibody bank using all of the proteins of bamboo at once to perform immune test on mice was investigated. So far 192 monoclonal antibodies have been isolated using the method of stage antibody screening with modifications on the antibody preparation and screening process. This method is a new concept and process. The purification process of antigen could be eliminated and many multiple monoclonal antibodies can be prepared in a short time. It is a platform combining proteomics and antibody bank. Many applications based on this platform can be performed. It can be applied to any biological system to generate antibody of any protein (except mice). Once the antibody bank is established, the making of the antibody chips can be conducted to provide research and development tools for cell biology, physiology, pathology and other related fields。 | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:14:28Z (GMT). No. of bitstreams: 1 ntu-95-D91623602-1.pdf: 8309323 bytes, checksum: 3c6842dfc22497d4ea0534b47208218e (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 目錄
中文摘要………………………………………………………………………………1 英文摘要………………………………………………………………………….….2 第一章 緒論…………………………………………………………………………..3 1.1 綠竹的生長………………………………………………………………..….3 1.1.1 綠竹筍細胞壁糖類生合成之探討…………………………………..4 1.1.2 綠竹筍生長時期糖類代謝可能之調控因子………………………..5 1.2 蛋白質體的興起及應用……………………………………………………...6 1.2.1 蛋白質體學技術……………………………………………………..6 1.2.2 生物晶片……………………………………………………………..7 1.3 單株抗體…………………………………………………………………… ..9 1.3.1 單株抗體的專一性及應用…………………………………………..9 1.3.2 目前單株抗體技術水平……………………………………………10 1.4 研究動機及目的…………………………………………………………….11 第二章 蛋白質體抗體庫……………………………………………………………13 2.1 建立第一階段之抗體庫…………………………………………………….13 2.1.1 抗原免疫及細胞融合………………………………………………13 2.1.2 抗體庫篩選…………………………………………………………14 2.2 第二階段抗體庫製備……………………………………………………….15 2.3 抗體庫之應用……………………………………………………………….16 2.4 抗體庫概念之創新………………………………………………………….17 第三章 綠竹筍生長期蛋白質體變化………………………………………………38 3.1 蛋白質體二維圖譜比對及鑑定…………………………………………….39 3.2 竹筍快速生長相關的醣類酵素…………………………………………….54 3.2.1 澱粉代謝相關酵素…………………………………………………..54 3.2.2 細胞壁多醣類合成相關酵素之消長………………………………..61 3.2.2.1 纖維素合成相關酵素………………………………………...62 3.2.2.2 半纖維素生合成相關酵素…………………………………...67 3.2.2.3 果膠生合成相關酵素………………………………………...70 3.2.3 糖解作用相關酵素…………………………………………………..77 3.2.3.1 Glucokinase, Fructokinase, Glc-6-P isomerase 及 Phosphoglucomutase……………………………………….78 3.2.3.2 Fructose biphosphate aldoase, Triosephosphate isomerase…….78 3.2.3.3 Glyceraldehyde-3-phosphate dehydrogenase…………………..79 3.2.3.4 Phosphoglycerate mutase, Enolase……………………………..80 3.2.4 檸檬酸循環相關酵素……………………………………………….89 3.2.4.1 Aconitase, Isocitrate dehydrogenase…………………………...90 3.2.4.2 Succinyl CoA ligase, Malate dehydrogenase…………………..90 3.2.4.3 Mitochondria ATP synthase.........................................................91 3.2.5 五碳醣磷酸路徑相關酵素.................................................................99 3.3 與竹筍生長相關胺基酸合成酵素...............................................................104 3.4 與竹筍生長相關的防禦機制及代謝酵素...................................................107 3.4.1 Phenylpropanoid pathway…………………………………………...107 3.4.2 Scavenge toxic reactive oxygen species pathway………………...…109 3.4.3 Glutathione S-transferase……………………………………………109 3.4.4 1,4-Benzoquinone reductase………………………………………...116 3.4.5 Chaperonins…………………………………………………………116 3.4.6 結論………………………………………………………………...117 3.5 與竹筍生長相關的蛋白質………………………………………………..120 3.5.1 G-protein…………………………………………………………….120 3.5.2 40S ribosomal protein S5及Elongation factor……………………..120 3.6 其他隨生長時期漸減蛋白質……………………………………………...124 3.6.1 Osmotin like protein…………………………………………………124 3.6.2 Protein disulfide isomerase…………………………………………..124 3.6.3 14-3-3 protein………………………………………………………..125 3.6.4 21kd polypeptide………………………………………………….....125 第四章 總結………………………………………………………………………..128 第五章 未來研究方向……………………………………………………………..130 第六章 材料與方法………………………………………………………………..131 6.1 一般分析方法…………………………………………………………...…131 6.1.1 一般蛋白質定量法…………………………………………………131 6.1.2 澱粉定量法…………………………………………………………132 6.1.3 綠竹筍pectin定量法……………………………………………….133 6.2 電泳檢定法……………………………………………………...…………135 6.2.1 不連續膠體電泳 (Native-PAGE)………………………………...135 6.2.2 SDS膠體電泳……………………………………………………..137 6.2.3 膠體染色法………………………………………………………..139 6.2.3.1 Coomassie Brilliant Blue R-250……………………………..139 6.2.3.2 硝酸銀蛋白質染色法…………………………………….....139 6.2.3.3 Pronceau S染色法…………………………………………...141 6.2.4 膠體乾片法………………………………………………………..141 6.3 酵素電泳活性染色法……………………………………………………...142 6.3.1 綠竹筍澱粉磷解脢電泳活性染色法(澱粉合成方向)……………142 6.3.2 綠竹筍澱粉磷解脢電泳活性染色法(澱粉磷解方向)……………143 6.3.3 綠竹筍澱粉磷解脢電泳活性染色法(澱粉磷解方向)……………143 6.3.4 綠竹筍H-SP電泳活性染色法……………………………………144 6.3.5 綠竹筍 | |
dc.language.iso | zh-TW | |
dc.title | 綠竹筍生長過程蛋白質體變化及其抗體庫之建立 | zh_TW |
dc.title | Study on the Proteome of the Fast Growing Bamboo Shoots and the Construction of its Antibody Bank | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 張富雄,吳建興(Jiann-Shing Wu),陳翰民(Han-Min Chen),楊健志(Chein-Chin Yang) | |
dc.subject.keyword | 綠竹筍,蛋白質體,抗體庫, | zh_TW |
dc.subject.keyword | Bambusa oldhamii,proteome,antibody bank, | en |
dc.relation.page | 190 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-25 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 8.11 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。