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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許翠瑛 | |
| dc.contributor.author | Hsin-Lan Kao | en |
| dc.contributor.author | 高歆嵐 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:51:25Z | - |
| dc.date.available | 2009-08-03 | |
| dc.date.copyright | 2006-08-03 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-25 | |
| dc.identifier.citation | Baer, R., A. T. Bankier, et al. (1984). 'DNA sequence and expression of the
B95-8 Epstein-Barr virus genome.' Nature 310(5974): 207-11. Brennan, B. M. (2006). 'Nasopharyngeal carcinoma.' Orphanet J Rare Dis 1(1): 23. Brooks, L., Q. Y. Yao, et al. (1992). 'Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts.' J Virol 66(5): 2689-97. Burke, A. P., T. S. Yen, et al. (1990). 'Lymphoepithelial carcinoma of the stomach with Epstein-Barr virus demonstrated by polymerase chain reaction.' Mod Pathol 3(3): 377-80. Burkitt, D. (1958). 'A sarcoma involving the jaws in African children.' Br J Surg 46(197): 218-23. Carmena, M. and W. C. Earnshaw (2003). 'The cellular geography of aurora kinases.' Nat Rev Mol Cell Biol 4(11): 842-54. Chang, Y., S. D. Cheng, et al. (2002). 'Chromosomal integration of Epstein-Barr virus genomes in nasopharyngeal carcinoma cells.' Head Neck 24(2): 143-50. Chang, Y. S., Y. S. Tyan, et al. (1990). 'Detection of Epstein-Barr virus DNA sequences in nasopharyngeal carcinoma cells by enzymatic DNA amplification.' J Clin Microbiol 28(11): 2398-402. Chen, J. Y., C. J. Chen, et al. (1989). 'Antibody to Epstein-Barr virus-specific DNase as a marker for field survey of patients with nasopharyngeal carcinoma in Taiwan.' J Med Virol 27(4): 269-73. Chen, J. Y., M. Y. Liu, et al. (1985). 'Antibody to Epstein-Barr virus-specific DNase as a marker for the early detection of nasopharyngeal carcinoma.' J Med Virol 17(1): 47-9. Chen, J. Y., M. Y. Liu, et al. (1982). 'Antibody to Epstein-Barr virus-specific DNase in patients with nasopharyngeal carcinoma.' Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 15(4): 255-61. Chen, M. R., M. Y. Liu, et al. (2001). 'Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients.' J Med Virol 64(1): 51-7. Cheng, Y., N. E. Poulos, et al. (1998). 'Functional evidence for a nasopharyngeal carcinoma tumor suppressor gene that maps at chromosome 3p21.3.' Proc Natl Acad Sci U S A 95(6): 3042-7. Chevallier-Greco, A., E. Manet, et al. (1986). 'Both Epstein-Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter.' EMBO J 5(12): 3243-9. Chien, Y. C., J. Y. Chen, et al. (2001). 'Serologic markers of Epstein-Barr virus infection and nasopharyngeal carcinoma in Taiwanese men.' N Engl J Med 345(26): 1877-82. Clough, W. (1979). 'Deoxyribonuclease activity found in Epstein--Barr virus producing lymphoblastoid cells.' Biochemistry 18(21): 4517-21. Countryman, J. and G. Miller (1985). 'Activation of expression of latent Epstein-Barr herpesvirus after gene transfer with a small cloned subfragment of heterogeneous viral DNA.' Proc Natl Acad Sci U S A 82(12): 4085-9. Countryman, J. and G. Miller (1985). 'Activation of expression of latent Epstein-Barr herpesvirus after gene transfer with a small cloned subfragment of heterogeneous viral DNA.' Proc Natl Acad Sci U S A 82(12): 4085-9. Cox, M. A., J. Leahy, et al. (1990). 'An enhancer within the divergent promoter of Epstein-Barr virus responds synergistically to the R and Z transactivators.' J Virol 64(1): 313-21. Dolyniuk, M., R. Pritchett, et al. (1976). 'Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus.' J Virol 17(3): 935-49. Duensing, S. and K. Munger (2004). 'Mechanisms of genomic instability in human cancer: insights from studies with human papillomavirus oncoproteins.' Int J Cancer 109(2): 157-62. Epstein, M. A., B. G. Achong, et al. (1964). 'Virus Particles in Cultured Lymphoblasts from Burkitt's Lymphoma.' Lancet 15: 702-3. Feldser, D. M., J. A. Hackett, et al. (2003). 'Telomere dysfunction and the initiation of genome instability.' Nat Rev Cancer 3(8): 623-7. Fenech, M. (2000). 'The in vitro micronucleus technique.' Mutat Res 455(1-2): 81-95. Fenech, M. (2002). 'Chromosomal biomarkers of genomic instability relevant to cancer.' Drug Discov Today 7(22): 1128-37. Feng, P., E. C. Ren, et al. (2000). 'Expression of Epstein-Barr virus lytic gene BRLF1 in nasopharyngeal carcinoma: potential use in diagnosis.' J Gen Virol 81(Pt 10): 2417-23. Foghsgaard, L. and M. Jaattela (1997). 'The ability of BHRF1 to inhibit apoptosis is dependent on stimulus and cell type.' J Virol 71(10): 7509-17. Gollin, S. M. (2005). 'Mechanisms leading to chromosomal instability.' Semin Cancer Biol 15(1): 33-42. Hammerschmidt, W. and B. Sugden (1988). 'Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus.' Cell 55(3): 427-33. Hampar, B., J. G. Derge, et al. (1971). 'Persistence of a repressed Epstein-Barr virus genome in Burkitt lymphoma cells made resistant to 5-bromodeoxyuridine.' Proc Natl Acad Sci U S A 68(12): 3185-9. Henderson, S., D. Huen, et al. (1993). 'Epstein-Barr virus-coded BHRF1 protein, a viral homologue of Bcl-2, protects human B cells from programmed cell death.' Proc Natl Acad Sci U S A 90(18): 8479-83. Henle, G. and W. Henle (1966). 'Studies on cell lines derived from Burkitt's lymphoma.' Trans N Y Acad Sci 29(1): 71-9. Henle, G., W. Henle, et al. (1968). 'Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis.' Proc Natl Acad Sci U S A 59(1): 94-101. Hoeijmakers, J. H. (2001). 'Genome maintenance mechanisms for preventing cancer.' Nature 411(6835): 366-74. Holley-Guthrie, E. A., E. B. Quinlivan, et al. (1990). 'The Epstein-Barr virus (EBV) BMRF1 promoter for early antigen (EA-D) is regulated by the EBV transactivators, BRLF1 and BZLF1, in a cell-specific manner.' J Virol 64(8): 3753-9. Hoshikawa, Y., Y. Satoh, et al. (2002). 'Evidence of lytic infection of Epstein-Barr virus (EBV) in EBV-positive gastric carcinoma.' J Med Virol 66(3): 351-9. Hsu, T. Y., Y. Chang, et al. (2005). 'Reactivation of Epstein-Barr virus can be triggered by an Rta protein mutated at the nuclear localization signal.' J Gen Virol 86(Pt 2): 317-22. Hsu, T. Y., C. Y. Pai, et al. (1992). 'Use of antigen expressed in bacteria for detection of EBV-specific thymidine kinase antibodies in sera from patients with nasopharyngeal carcinoma.' J Med Virol 38(3): 214-9. Huang, D. P., J. H. Ho, et al. (1989). 'Cytogenetics of undifferentiated nasopharyngeal carcinoma xenografts from southern Chinese.' Int J Cancer 43(5): 936-9. Huang, D. P., K. W. Lo, et al. (1991). 'Loss of heterozygosity on the short arm of chromosome 3 in nasopharyngeal carcinoma.' Cancer Genet Cytogenet 54(1): 91-9. Huang, J., H. Chen, et al. (2003). 'Lytic viral replication as a contributor to the detection of Epstein-Barr virus in breast cancer.' J Virol 77(24): 13267-74. Jones, J. F., S. Shurin, et al. (1988). 'T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections.' N Engl J Med 318(12): 733-41. Jordan, M. A., D. Thrower, et al. (1992). 'Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis.' J Cell Sci 102 ( Pt 3): 401-16. Kallin, B., L. Sternas, et al. (1985). 'Purification of Epstein-Barr virus DNA polymerase from P3HR-1 cells.' J Virol 54(2): 561-8. Kao, S. Y. and S. J. Marriott (1999). 'Disruption of nucleotide excision repair by the human T-cell leukemia virus type 1 Tax protein.' J Virol 73(5): 4299-304. Kenney, S., E. Holley-Guthrie, et al. (1989). 'The Epstein-Barr virus BMLF1 promoter contains an enhancer element that is responsive to the BZLF1 and BRLF1 transactivators.' J Virol 63(9): 3878-83. Kieff, E. (1996). Epstein-Barr virus and it's replication. Kikuta, H., Y. Taguchi, et al. (1988). 'Epstein-Barr virus genome-positive T lymphocytes in a boy with chronic active EBV infection associated with Kawasaki-like disease.' Nature 333(6172): 455-7. Kirsch-Volders, M., A. Elhajouji, et al. (1997). 'The in vitro micronucleus test: a multi-endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction.' Mutat Res 392(1-2): 19-30. Littler, E. and J. R. Arrand (1988). 'Characterization of the Epstein-Barr virus-encoded thymidine kinase expressed in heterologous eucaryotic and procaryotic systems.' J Virol 62(10): 3892-5. Liu, C., N. D. Sista, et al. (1996). 'Activation of the Epstein-Barr virus DNA polymerase promoter by the BRLF1 immediate-early protein is mediated through USF and E2F.' J Virol 70(4): 2545-55. Liu, M. T., Y. T. Chang, et al. (2005). 'Epstein-Barr virus latent membrane protein 1 represses p53-mediated DNA repair and transcriptional activity.' Oncogene 24(16): 2635-46. Liu, M. T., Y. R. Chen, et al. (2004). 'Epstein-Barr virus latent membrane protein 1 induces micronucleus formation, represses DNA repair and enhances sensitivity to DNA-damaging agents in human epithelial cells.' Oncogene 23(14): 2531-9. Liu, M. Y., Y. L. Chang, et al. (1997). 'Evaluation of multiple antibodies to Epstein-Barr virus as markers for detecting patients with nasopharyngeal carcinoma.' J Med Virol 52(3): 262-9. Liu, M. Y., W. H. Chou, et al. (1989). 'Antibody against Epstein-Barr virus DNA polymerase activity in sera of patients with nasopharyngeal carcinoma.' J Med Virol 28(2): 101-5. Liu, M. Y., Y. Y. Shih, et al. (1998). 'Antibody against the Epstein-Barr virus BHRF1 protein, a homologue of Bcl-2, in patients with nasopharyngeal carcinoma.' J Med Virol 56(3): 179-85. Lo, K. W., P. M. Teo, et al. (2000). 'High resolution allelotype of microdissected primary nasopharyngeal carcinoma.' Cancer Res 60(13): 3348-53. Lo, Y. M., L. Y. Chan, et al. (1999). 'Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal carcinoma.' Cancer Res 59(6): 1188-91. Majone, F., O. J. Semmes, et al. (1993). 'Induction of micronuclei by HTLV-I Tax: a cellular assay for function.' Virology 193(1): 456-9. Martel-Renoir, D., V. Grunewald, et al. (1995). 'Qualitative analysis of the expression of Epstein-Barr virus lytic genes in nasopharyngeal carcinoma biopsies.' J Gen Virol 76 ( Pt 6): 1401-8. Maser, R. S. and R. A. DePinho (2003). 'Take care of your chromosomes lest cancer take care of you.' Cancer Cell 3(1): 4-6. Matsuoka, A., N. Yamazaki, et al. (1992). 'Evaluation of the micronucleus test using a Chinese hamster cell line as an alternative to the conventional in vitro chromosomal aberration test.' Mutat Res 272(3): 223-36. Miller, R. L., R. Glaser, et al. (1977). 'Studies of an Epstein-Barr virus-induced DNA polymerase.' Virology 76(2): 494-502. Minhas, K. M., B. Singh, et al. (2003). 'Spindle assembly checkpoint defects and chromosomal instability in head and neck squamous cell carcinoma.' Int J Cancer 107(1): 46-52. Norppa, H. and G. C. Falck (2003). 'What do human micronuclei contain?' Mutagenesis 18(3): 221-33. Old, L. J., E. A. Boyse, et al. (1966). 'Precipitating Antibody in Human Serum to an Antigen Present in Cultured Burkitt's Lymphoma Cells.' Proc Natl Acad Sci U S A 56(6): 1699-1704. Oudejans, J. J., A. J. van den Brule, et al. (1995). 'BHRF1, the Epstein-Barr virus (EBV) homologue of the BCL-2 protooncogene, is transcribed in EBV-associated B-cell lymphomas and in reactive lymphocytes.' Blood 86(5): 1893-902. Philpott, S. M. and G. C. Buehring (1999). 'Defective DNA repair in cells with human T-cell leukemia/bovine leukemia viruses: role of tax gene.' J Natl Cancer Inst 91(11): 933-42. Raab-Traub, N. (2002). 'Epstein-Barr virus in the pathogenesis of NPC.' Semin Cancer Biol 12(6): 431-41. Rickinson, A. B. a. K., E. (2001). Field's Virology. 4th edn. Knipe DM and Howley PM（eds）. Lippincott Williams & Wilkins Publishers: Philadelphia, pp. 2575-2627. Sancar, A., L. A. Lindsey-Boltz, et al. (2004). 'Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints.' Annu Rev Biochem 73: 39-85. Shao, J. Y., X. M. Huang, et al. (2001). 'Loss of heterozygosity and its correlation with clinical outcome and Epstein-Barr virus infection in nasopharyngeal carcinoma.' Anticancer Res 21(4B): 3021-9. Sixbey, J. W., E. H. Vesterinen, et al. (1983). 'Replication of Epstein-Barr virus in human epithelial cells infected in vitro.' Nature 306(5942): 480-3. Spruck, C. H., 3rd, Y. C. Tsai, et al. (1992). 'Absence of p53 gene mutations in primary nasopharyngeal carcinomas.' Cancer Res 52(17): 4787-90. Stopper, H., M. Full, et al. (1997). 'Micronucleus induction by neocarzinostatin and methyl methanesulfonate in ionizing radiation--sensitive Chinese hamster V79 cell mutants.' Mutat Res 383(2): 107-12. Sun, Y., G. Hegamyer, et al. (1992). 'An infrequent point mutation of the p53 gene in human nasopharyngeal carcinoma.' Proc Natl Acad Sci U S A 89(14): 6516-20. Sun, Y., A. Hildesheim, et al. (1995). 'No point mutation but decreased expression of the p16/MTS1 tumor suppressor gene in nasopharyngeal carcinomas.' Oncogene 10(4): 785-8. Swenson, J. J., A. E. Mauser, et al. (1999). 'The Epstein-Barr virus protein BRLF1 activates S phase entry through E2F1 induction.' J Virol 73(8): 6540-50. Takada, K. and Y. Ono (1989). 'Synchronous and sequential activation of latently infected Epstein-Barr virus genomes.' J Virol 63(1): 445-9. Tovey, M. G., G. Lenoir, et al. (1978). 'Activation of latent Epstein-Barr virus by antibody to human IgM.' Nature 276(5685): 270-2. Trumper, P. A., M. A. Epstein, et al. (1977). 'Isolation of infectious EB virus from the epithelial tumour cells of nasopharyngeal carcinoma.' Int J Cancer 20(5): 655-62. Walling, D. M., S. N. Edmiston, et al. (1992). 'Coinfection with multiple strains of the Epstein-Barr virus in human immunodeficiency virus-associated hairy leukoplakia.' Proc Natl Acad Sci U S A 89(14): 6560-4. Wei, W. I. and J. S. Sham (2005). 'Nasopharyngeal carcinoma.' Lancet 365(9476): 2041-54. Weiss, L. M., J. G. Strickler, et al. (1987). 'Epstein-Barr viral DNA in tissues of Hodgkin's disease.' Am J Pathol 129(1): 86-91. Xie, A., N. Puget, et al. (2004). 'Control of sister chromatid recombination by histone H2AX.' Mol Cell 16(6): 1017-25. Yoshizaki, T., H. Miwa, et al. (2000). 'Elevation of antibody against Epstein-Barr virus genes BRLF1 and BZLF1 in nasopharyngeal carcinoma.' J Cancer Res Clin Oncol 126(2): 69-73. Zacny, V. L., J. Wilson, et al. (1998). 'The Epstein-Barr virus immediate-early gene product, BRLF1, interacts with the retinoblastoma protein during the viral lytic cycle.' J Virol 72(10): 8043-51. Zalani, S., E. Holley-Guthrie, et al. (1996). 'Epstein-Barr viral latency is disrupted by the immediate-early BRLF1 protein through a cell-specific mechanism.' Proc Natl Acad Sci U S A 93(17): 9194-9. Zeng, Y., L. G. Zhang, et al. (1982). 'Serological mass survey for early detection of nasopharyngeal carcinoma in Wuzhou City, China.' Int J Cancer 29(2): 139-41. zur Hausen, H., F. J. O'Neill, et al. (1978). 'Persisting oncogenic herpesvirus induced by the tumour promotor TPA.' Nature 272(5651): 373-5. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32472 | - |
| dc.description.abstract | Epstein-Barr virus（EBV）是第一個被認為與人類腫瘤疾病有關的人類DNA 病毒。過去研究已發現EB 病毒感染與許多人類惡性腫瘤疾病相關，其中之一就是鼻咽癌（Nasopharyngeal carcinoma, NPC）。鼻咽癌為發生於鼻咽部上皮細胞的惡性腫瘤，患者主要分佈在台灣及中國東南部。在鼻咽癌患者組織中，利用細胞遺傳學（cytogenetic）的研究方法分析，發現有廣泛的染色體異常
之情形。這樣的結果顯示基因體不穩定（genomic instability）可能促使鼻咽癌的形成。因此本研究希望探討EB 病毒特早期活化子Rta 是否會造成宿主細胞基因體不穩定？並深入瞭解Rta 藉由哪些機制造成宿主細胞基因體不穩定。 首先， 我們證明在293 衍生細胞株中， Rta 的確可以誘使微核 （micronucleus，MN）的形成。其次，在HEp2 與HEp2 衍生細胞株中，則發現Rta 會加速G2/M phase 到G1 phase 細胞週期的進行，並且伴隨著微核形成的增加。此外，我們也發現Rta 可能造成DNA 雙股斷裂（DNA double strandbreak）。然而，在宿主細胞質體再活化試驗（host cell reactivation assay）中，卻發現Rta 可促進細胞修復DNA 的能力。綜合以上的實驗結果，我們證明了EB 病毒轉活化子Rta 在人類上皮細胞中，可以促使微核的形成、DNA 股斷裂以及加速細胞週期M phase 之進行。 | zh_TW |
| dc.description.abstract | Epstein-Barr virus（EBV）was the first human DNA virus to be proposed as a tumor virus and EBV infection is associated with the development of many types of malignancies, including nasopharyngeal carcinoma（NPC）. NPC is one of the most common cancer in Taiwan and Southeast China. The cytogenetic studies have demonstrated broad chromosomal abnormalities in NPC tissues. It is suggested that chromosomal instability may play an
essential role in the development of NPC, just as in many solid tumors. We investigated whether EBV immediate early gene BRLF1, Rta, contribute to the induction of genomic instability in the human epithelial cells. At first, we demonstrated that EBV Rta could induce micronucleus（MN)formation in 293 inducible stable clone. Secondly, we found EBV Rta accelerates G2/M-to-G1 cell cycle progression in transfected HEp2 cells and HEp2 inducible stable clone. This accelerated cell cycle M phase progression was accompanied with the increase micronuclei formation. Furthermore, we demonstrated that Rta induces DNA strand breaks. However, Rta still enhances the ability of DNA repair in host cell reactivation（HCR）assay. Taken together, these studies suggest that EBV Rta enhances micronucleus formation, DNA strand break and accelerates M phase progression in human epithelial cells. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:51:25Z (GMT). No. of bitstreams: 1 ntu-95-R93445123-1.pdf: 2823303 bytes, checksum: c2a2473ba8e8190b8e99b806e08b2612 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 目錄
中文摘要1 英文摘要 2 序論3 材料與方法13 實驗結果23 討論32 圖表38 參考文獻63 | |
| dc.language.iso | zh-TW | |
| dc.subject | 微核 | zh_TW |
| dc.subject | 細胞週期 | zh_TW |
| dc.subject | EB病毒轉活化子Rta | zh_TW |
| dc.subject | EBV Rta | en |
| dc.subject | Cell cycle | en |
| dc.subject | Micronucleus | en |
| dc.title | 探討EB 病毒轉活化子Rta 在人類上皮細胞中，促使微核形成、DNA 股斷裂及加速細胞週期M phase進行之研究 | zh_TW |
| dc.title | EBV Rta Enhances Micronucleus Formation, DNA Strand Break and Accelerates M Phase Progression in Human Epithelial Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳振陽,董馨蓮 | |
| dc.subject.keyword | EB病毒轉活化子Rta,微核,細胞週期, | zh_TW |
| dc.subject.keyword | EBV Rta,Micronucleus,Cell cycle, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-26 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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