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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張上鎮(Shang-Tzen Chang) | |
| dc.contributor.author | Chin-Yu Huang | en |
| dc.contributor.author | 黃志煜 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:42:12Z | - |
| dc.date.available | 2011-07-31 | |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-26 | |
| dc.identifier.citation | 王松永 （1983）商用木材。林產事業協會。pp 103-104。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32312 | - |
| dc.description.abstract | 本研究利用薄層層析、管柱層析及高效能液相層析等技術，分離純化相思樹花抽出成分，並利用DPPH自由基清除、超氧自由基清除、脂質過氧化以及還原力等方法評估其抗氧化活性，且測定總酚類含量以及總黃酮類含量。所得到之抗氧化活性成分，則進一步運用質譜分析以及核磁共振光譜分析等技術鑑定化合物之結構。由相思樹花甲醇粗抽出物之二氯甲烷可溶部、正丁醇可溶部及水可溶部之抗氧化活性試驗結果顯示，正丁醇可溶部具有最好的抗氧化活性，除了脂質過氧化外，不論在DPPH自由基或超氧自由基清除能力以及還原能力，正丁醇可溶部皆有很好的效果，活性比原甲醇粗抽出物好，而且正丁醇可溶部之總酚類含量（263.2 mg GAE /g）最高，因此，續以管柱層析將正丁醇可溶部分離成15個次分離部。其中，Bu3、Bu4及Bu5次分離部具有很好的DPPH自由基清除能力，並含有很高的黃酮類含量，而又以Bu4次分離部之抗氧化活性最強且黃酮類含量最高。次分離部中共分離出6個黃酮類化合物，包括：Myricitrin、Quercitrin、Europetin-3-rhamnoside、Kaempferol-3-rhamnoside、Rhamnetin-3-glucoside及Rhamnetin-3-rhamnoside六個化合物，其中以 Myricitrin及Europetin-3-rhamnoside的抗氧化活性最好。而正丁醇可溶部水解後之乙酸乙酯可溶部具有很強的抗氧活性，其中所含的成分為Myricetin、Quercetin、Europetin以及Keampferol。此外，將相思樹花與市面上之花茶（洋甘菊、桂花、玫瑰及茉莉）的熱水抽出物進行抗氧化活性比較，結果顯示相思樹花之抗氧化能力與洋甘菊相同，並且優於茉莉。 | zh_TW |
| dc.description.abstract | In this study, thin layer chromatography (TLC), column chromatography (CC) and high performance liquid chromatography (HPLC) were employed to separate and purify the constituents of the methanolic extracts of Acacia confusa flowers. Furthermore, the antioxidant activities were evaluated by DPPH radical and superoxide radical scavenging assays, lipid peroxidation assay and reducing power assay. Total phenolic contents and total flavonoid contents were also evaluated. In addition, the chemical structure of active constituents from A. confusa flowers was identified by the analyses of mass spectroscopy and nucleus magnetic resonance spectroscopy (NMR), etc. Results demonstrated that among all soluble fractions (CH2Cl2, BuOH and Water) of methanolic extracts of A. confusa flowers, BuOH fraction has the best activities. Though the inhibition of lipid peroxidaiton of BuOH fraction is low, the DPPH radical and superoxide radical scavenging activities are the highest, even better than that of crude methanolic extracts. Total phenolic content of BuOH fraction (263.2 mg GAE /g) is also the highest. BuOH fraction was then separated into 15 subfractions which were tested by DPPH radical scavenging assay for antioxidant activity and their total flavonoid contents were also evaluated. Subfractions Bu3, Bu4 and Bu5 showed a strong antioxidant activity, especially subfraction Bu4, which also contains the most flavonoids. Six pure flavonoids, myricitrin, quercitrin, europetin-3-rhamnoside, kaempferol-3-rhamnoside, rhamnetin-3-glucoside and rhamnetin-3-rhamnoside were isolated from BuOH fraction of A. confusa flowers. Among them, myricitrin and europetin-3-rhamnoside are the best antioxidant compounds. The EA fraction hydrolyzed from BuOH fraction in which major constituents are myricetin, quercetin, europetin and keampferol has remarkable antioxidant activities. Furthermore, the antioxidant activities of hot water extracts from various flowers (A. confusa, Matricaria chamomilia, Osmanthus fragrans, Rose rugosa and Jasminum sambac) were also compared. Results obtained indicated that antioxidant activities of A. confusa flowers are comparable to M. chamomilia and better than J. sambac. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:42:12Z (GMT). No. of bitstreams: 1 ntu-95-R92625012-1.pdf: 1598805 bytes, checksum: 68d3f4d1e69a8b5587627a87bdfc596d (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 目錄 ............................................... I
表目次 ............................................. IV 圖目次 ............................................. VII 摘要 ............................................... XI Abstract ........................................... XII 壹、前言 ........................................... 1 貳、文獻回顧 ....................................... 3 一、相思樹屬植物之簡介 ........................... 3 （一） 已進行抗氧化活性評估之相思樹屬樹種 ...... 4 （二） 未進行抗氧化活性評估之相思樹屬樹種 ...... 4 二、活性氧之生成與傷害 ........................... 6 （一）活性氧種類 ............................... 6 1. 超氧自由基 ........................... 7 2. 過氧化氫 ............................. 7 3. 氫氧自由基 ........................... 7 4. 單峰氧 ............................... 8 （二）活性氧的來源 ............................. 8 1. 生物合成與新陳代謝 .................. 8 2. 發炎反應 ............................. 8 3. 環境因素 ............................. 9 三、氧化壓力的形成與影響 ......................... 9 四、氧化傷害之防禦 ............................... 10 五、黃酮類化合物之生合成、分佈與功能 ............. 11 （一）黃酮類化合物之生合成 ..................... 12 （二）黃酮類化合物於植物中之分佈 ............... 14 （三）黃酮類化合物之功能 ....................... 15 参、材料與方法 ...................................... 17 一、試驗材料 ..................................... 17 （一）相思樹花 ................................. 17 （二）花茶 ..................................... 17 （三）試驗藥品與溶劑 ........................... 17 二、試驗方法 ..................................... 18 （一）相思樹花抽出物萃取 ....................... 18 （二）相思樹花甲醇抽出物之分離純化 ............. 18 1. 液相－液相分配 ....................... 18 2. 薄層層析與管柱層析 ................... 18 3. 高效能液相層析 ....................... 19 （三）化合物結構鑑定 ........................... 20 1. 核磁共振分析 ......................... 20 2. 質譜鑑定 ............................. 21 3. 紅外線光譜鑑定 ....................... 21 4. 紫外光-可見光光譜測定 ................ 21 （四）相思樹花甲醇抽出物正丁醇可溶部之水解 ..... 21 （五）相思樹花甲醇抽出物之抗氧化活性試驗 ....... 22 1. DPPH自由基清除試驗 ................... 22 2. 超氧自由基清除試驗 ................... 22 3. 抑制脂質過氧化試驗 ................... 23 4. 還原力試驗 ........................... 24 （六）相思樹花甲醇抽出物之總酚類化合物含量測定 . 24 （七）相思樹花甲醇抽出物之總黃酮類化合物含量測定 25 肆、結果與討論 ..................................... 26 一、相思樹花各可溶部之活性篩選 ................... 26 （一）清除DPPH自由基之能力 ..................... 27 （二）清除超氧自由基之能力 ..................... 29 （三）還原能力 ................................. 30 （四）抑制脂質過氧化之能力 ..................... 31 （五）總酚量測定 ............................... 33 二、相思樹花正丁醇可溶部之分離與活性篩選 ......... 34 三、相思樹花正丁醇可溶部各次分離部之成分分析 ..... 36 四、相思樹花正丁醇可溶部各次分離部之總黃酮類含量 . 38 五、相思樹花正丁醇可溶部抗氧化活性成分之分離與純化 39 六、相思樹花主要黃酮類化合物之含量及其抗氧化活性 . 66 七、相思樹花正丁醇可溶部水解產物之抗氧化活性 ..... 70 （一）相思樹花正丁醇可溶部水解後之乙酸乙酯可溶部與水 可溶部之抗氧化活性 ....................... 70 （二）相思樹花正丁醇可溶部水解後之乙酸乙酯可溶部中之 主要成分與活性 ........................... 72 八、各種花茶之抗氧化效果 ......................... 77 伍、結論 ............................................ 82 陸、參考文獻 ........................................ 84 | |
| dc.language.iso | zh-TW | |
| dc.title | 相思樹花之抗氧化活性成分分析與鑑定 | zh_TW |
| dc.title | Identification and Analysis of Antioxidant Constituents from Acacia confusa Flowers | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李文昭(Wen-Jau Lee),蘇裕昌(Yu-Chang Su),王升陽(Sheng-Yang Wang),張惠婷(Hui-Ting Chang) | |
| dc.subject.keyword | 相思樹,花,抽出成分,抗氧化,黃酮類化合物, | zh_TW |
| dc.subject.keyword | Acacia confusa,flower,extractives,antioxidant activity,flavonoids, | en |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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