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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳益群(Yi-Chun Wu) | |
| dc.contributor.author | Min-Jan Chiu | en |
| dc.contributor.author | 邱敏然 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:36:36Z | - |
| dc.date.available | 2006-09-17 | |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-26 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32204 | - |
| dc.description.abstract | CCT (Chaperonin contain T-complex polypeptide 1又名TriC)是真核生物特有的chaperonin，由8個次單元組成，共組一個16-mer的雙環結構複合體，協助新合成蛋白的摺疊或是變性蛋白的再褶疊。目前已知actin，tubulin，以及其它蛋白經過CCT摺疊而活化。且陸續有新的蛋白發現和CCT作用，其中，許多是具有WD repeat doamain的蛋白。CCT各次單元序列之間的差異，暗示各單元扮演不同的功能，可能和不同受質作用。我们在線蟲( Caenorhabditis elegans)分別對各次單元進行knockdown，發現這些基因皆為早期胚胎發育所需，細胞分裂時，如紡錘絲的形成，染色體的分離，細胞質分裂等過程都受到影響，表現pleiotropic的性狀。我们的觀察顯示tubulin的表現量可能減少。然而，我们發現knockdown後，線蟲會滯留在幼蟲時期，生殖腺退化，且消化道澎大並充滿未消化的細菌，對機械性外力敏感。這些性狀和let-363(CeTOR)突變株有相似之處。TOR是細胞生長的中心調控者，負責生合成核醣體，轉譯等作用。因此，我们猜測CCT可能影響細胞的生長與代謝。 | zh_TW |
| dc.description.abstract | Eukayotic chaperonin CCT (Chaperonin contain T-complex polypeptide also named TriC)，established by 8 subunits，is a 16- mer double-ring complex. CCT contributes to the folding of newly synthesized proteins and the refolding of denatured proteins. Actin, tubulin and several other proteins are well known activated by CCT folding. New proteins are still found to interact with CCT, and many of them WD repeat proteins. CCT subunits are different in protein sequences, suggesting specification between them. We knockdown the subunits in Caenorhabditis elegans and found them all essential for early embryogenesis. We found defects in spindle fiber formation，chromosome segregation，cytokinesis and others，indicating a pleiotropic effect. Our observation also suggests deficiency in tubulin. However, the progeny arrest in larval stage with degenerate gonads and intestinal lumens enlarged by undigested bacteria. And they are sensitive to mechanical pressure. Similar phenotypes are in let-363 (CeTOR) mutant. TOR is the central controller of cell growth. We suggest a role of CCT in cell growth and metabolism. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:36:36Z (GMT). No. of bitstreams: 1 ntu-95-R91225016-1.pdf: 3102366 bytes, checksum: 6ce2ce81c13daa0eca2a9f23ed49ce01 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 中文摘要 1
Abstract 2 引言 3 CCT是真核生物特有的Chaperonin 3 細胞骨架蛋白actin及tubulin是CCT的受質 3 目前其他已知的CCT受質 4 CCT和ubiquitin ligase、WD repeat蛋白之間的關聯性 4 WD repeat 蛋白質家族 5 Ubiquitin 蛋白質降解系統 6 CCT與TOR signaling pathway之間的關聯 7 TOR signaling 的介紹 8 CCT與疾病的關聯 11 細胞分裂 12 線蟲早期胚胎為研究細胞分裂的理想系統 14 材料與方法 16 線蟲 16 Plasmid 製備 16 RNA-mediated interference 17 Time-lapse 螢光顯微鏡 17 性狀分析 17 結果 18 CCT是高度保守性的基因 18 CCT基因表現模式 18 CCT RNAi 降低產卵率 19 CCT RNAi 造成胚胎死亡 20 CCT RNAi 造成幼蟲生長遲滯 20 CCT RNAi 造成生殖腺發育退化 20 腸道內堆積大量bacteria且口徑加大 21 Pleiotropic defect in early stage embryo 21 Spindle fiber 無法形成 21 其它常見早期胚胎性狀 23 討論 24 CCT RNAi 和CeTOR mutant (let -363)性狀相似 24 CCT影響早期胚胎發育 25 CCT影響成蟲及幼蟲發育 26 CCT可能造成tubulin aggregation 27 疾病治療的可能性 28 圖 表 29 參考文獻 41 | |
| dc.language.iso | zh-TW | |
| dc.subject | 線蟲 | zh_TW |
| dc.subject | 細胞骨架 | zh_TW |
| dc.subject | 細胞週期 | zh_TW |
| dc.subject | 細胞生長 | zh_TW |
| dc.subject | 胚胎發育 | zh_TW |
| dc.subject | WD repeat | en |
| dc.subject | C. elegans | en |
| dc.subject | CCT | en |
| dc.subject | RNA intreference | en |
| dc.subject | embryogenesis | en |
| dc.subject | cell growth | en |
| dc.subject | tubulin | en |
| dc.subject | TOR | en |
| dc.subject | Chaperonin | en |
| dc.title | CCT chaperonin在線蟲早期胚胎發育的功能分析 | zh_TW |
| dc.title | Functional analysis of CCT chaperonin
in early embryogenesis in Caenorhabditis elegans | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蘇銘燦(Ming-Tsan Su),李銘仁(Ming-Jen Lee) | |
| dc.subject.keyword | 線蟲,細胞骨架,胚胎發育,細胞生長,細胞週期, | zh_TW |
| dc.subject.keyword | C. elegans,CCT,Chaperonin,TOR,tubulin,embryogenesis,RNA intreference,WD repeat,cell growth, | en |
| dc.relation.page | 46 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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