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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳金洌 | |
| dc.contributor.author | Wen-Zheng Lin | en |
| dc.contributor.author | 林文政 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:24:49Z | - |
| dc.date.available | 2008-07-31 | |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-27 | |
| dc.identifier.citation | M.R. Briggs, C. Yokoyama, X. Wang, M.S. Brown and J.L. Goldstein(1993), Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter I. Identification of the protein and delineation of its target nucleotide sequence. J. Biol. Chem. 268, pp. 14490–14496
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Hobbs (1995), Structure of human gene encoding sterol regulatory element binding protein-1 (SREBF1) and localization of SREBF1 and SREBF2 to chromosomes 17p11.2 and 22q13. Genomics 25, pp. 667–673 Shimomura, H. Shimano, J.D. Horton, J.L. Goldstein and M.S. Brown (1997), Differential expression of exons 1a and 1c in mRNAs for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells. J. Clin. Invest. 99, pp. 838–845 R. Sato, J. Yang, X. Wang, M.J. Evans, Y.K. Ho, J.L. Goldstein and M.S. Brown (1994), Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element binding protein-1 (SREBP-1). J. Biol. Chem. 269, pp. 17267–17273 X. Wang, R. Sato, M.S. Brown, X. Hua and J.L. Goldstein(1994),, SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell 77 pp. 53–62 X. Hua, J. Sakai, M.S. Brown and J.L. Goldstein (1996), Regulated cleavage of sterol regulatory element binding proteins (SREBPs) requires sequences on both sides of the endoplasmic reticulum membrane. J. Biol. Chem. 271, pp. 10379–10384 Sakai, J., Duncan, E. A., Rawson, R. B., Hua, X., Brown, M. S., and Goldstein, J. L. (1996) Cell 85, 1037-1046 J.L. Goldstein and M.S. Brown, Regulation of the mevalonate pathway. Nature 343 (1990), pp. 425–430. T.F. Osborne(1995), Transcriptional control mechanisms in the regulation of cholesterol balance. Crit. Rev. Eukaryot. Gene Expr. 5, pp. 317–335 G. Guan, G. Jiang, R.L. Koch and I. Shechter(1995), Molecular cloning and functional analysis of the promoter of the human squalene synthase gene. J. Biol. Chem. 270, pp. 21958–21965 Barba, G., Harper, F., Harada, T., Kohara, M., Goulinet, S., Matsuura, Y., Eder, G., Schaff, Z., Chapman, M.J., Miyamura, T., and Brechot, C. (1997) Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets. Proc. Natl. Acad. Sci. USA 94, 1200–1205 J. Ericsson, S.M. Jackson, B.C. Lee and P.A. Edwards (1996), Sterol regulatory element binding protein binds to a cis element in the promoter of the farnesyl diphosphate synthase gene. Proc. Natl. Acad. Sci. USA 93, pp. 945–950. Ray, R.B., Lagging, L.M., Meyer, K., and Ray, R. (1996) Hepatitis C virus core protein cooperates with ras and transforms primary rat embryo fibroblasts to tumorigenic phenotype. J. Virol. 70, 4438–4443 Yoshida, T., Hanada, T., Tokuhisa, T., Kosai, K., Sata, M., Kohara, M., and Yoshimura, A. (2002) Activation of STAT3 by the hepatitis C virus core protein leads to cellular transformation. J. Exp. Med. 196, 641–653 Yokoyama, C., Wang, X., Briggs, M. R., Admon, A., Wu, J., Hua, X., Goldstein, J. L., and Brown, M. S. (1993) Cell 75, 187-197 Hua, X., Yokoyama, C., Wu, J., Briggs, M. R., Brown, M. 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Goldstein(1996), Overproduction of cholesterol and fatty acids causes massive liver enlargement in transgenic mice expressing truncated SREBP-1a. J. Clin. Invest. 98, pp. 1575–1584. Jay D. Horton, Iichiro Shimomura, Shinji Ikemoto, Yuriy Bashmakov, and Robert E. Hammer( 2003), Overexpression of Sterol Regulatory Element-binding Protein-1a inMouse Adipose Tissue Produces Adipocyte Hypertrophy, Increased Fatty Acid Secretion, and Fatty Liver, j. of Bio. Chem, Vol. 278, No. 38, Issue of September 19, pp. 36652–36660, Nathan R. Qi, Jiaming Wang, Vaclav Zidek, Vladimir Landa, Petr Mlejnek, Ludmila Kazdova´,Michal Pravenec, Theodore W. Kurtz, (2005) A New Transgenic Rat Model of Hepatic Steatosis and the Metabolic Syndrome, Hypertension.; 45:1004-1011. Nicolas DIF, Vanessa Euthine, Estelle GONNET, Martine LAVILLE, Hubert VIDAL and Etienne LEFAI(2006). Insulin activates human Sterol Regulatory Element-Binding Protein-1c (SREBP-1c) promoter through SRE motifs, Biochemical Journal, 10 Jul Aseervatham Anusha Amali, Ravikumar Deepa Rekha, Cliff Ji-Fan Lin, Wei-Lun Wang, Hong-Yi Gong, Gour-Mour Her & Jen-Leih Wu (2006), Thioacetamide induced liver damage in zebrafish embryo as a disease model for steatohepatitis, Journal of Biomedical Science 13: 225–232 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31928 | - |
| dc.description.abstract | 固醇調控序列結合蛋白(SREBP)是一群與膽固醇脂肪酸的合成有關的轉錄因子，在哺乳類動物中可被分為三種型，分別為SREBP-1a，SREBP-1c與SREBP-2。他們可藉由活化許多的基因如與合成膽固醇有關的HMG CoA合成酶與還原酶、squalene合成酶以及與合成脂肪酸有關的乙醯輔酶A羧化酶、脂肪酸合成酶等來進行膽固醇與脂肪酸的生合成調控。在老鼠實驗模組中，老鼠可藉由過度表現第一型固醇調控序列結合蛋白而產生脂肪肝與有較大肝臟的表現型。在我們的研究中，我們已經選殖到斑馬魚的第一型固醇調控序列結合蛋白 (SREBP1) 全長序列（其中包括3540個鹼基以及1105個氨基酸序列）以及其長度為3006個鹼基的啟動子序列，包含兩個固醇調控序列結合蛋白與一個C/EBP的結合位置。在斑馬魚的第一型固醇調控序列結合蛋白啟動子的活性實驗中，轉錄因子：成熟型之第一型固醇調控序列結合蛋白、C/EBPα 以及C/EBPβ可以活化第一型固醇調控序列結合蛋白啟動子分別約為六倍、三倍與四倍，但是肝細胞核因子1α、1β和1γ則沒有明顯活化。此外，分別將綠色螢光肝臟轉基因斑馬魚以及人類C型肝炎病毒輔蛋白轉殖斑馬分別處理肝毒素TAA三週及一週後，斑馬魚的第一型固醇調控序列結合蛋白的表現會被明顯地誘導起來。 | zh_TW |
| dc.description.abstract | sterol regulatory element binding proteins (SREBPs) are transcription factors involved in fatty acid and cholesterol biosynthesis. Three isoforms: SREBP-1a, SREBP-1c, and SREBP-2 are found in mammals. They can turn on the fatty acid and cholesterol biosynthesis by activating multiple genes like acetyl CoA carboxylase(ACC) and fatty acid synthase(FAS) relating to fatty acid biosynthesis and 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) synthase, HMG CoA reductase, and squalene synthase relating to cholesterol biosynthesis. In mouse model, liver steatosis and enlarged liver by overexpressing SREBP-1 can be observed. In my study, I had cloned the full length zebrafish ( Danio rerio) SREBP1 (3540 bp) cDNA encoding 1105 amino acids and 3Kb promoter of zebrafish SREBP1 gene with two putative SREBP1 responsive elements (SRE) and one CCATT/enhancer binding protein (C/EBP) binding site. From the promoter assay of 3Kb zebrafish SREBP1 promoter, the transcription factors mature SREBP1, C/EBPα and C/EBPβ could activate the expression of SREBP1 gene at about 6, 3 and 4 folds, respectively whereas the hepatocyte nuclear factor 1α(HNF1α), 1β, and 1γ didn’t show significant transactivation activities. In addition to, zebrafish SREBP1 gene can be significantly induced by hepatotoxin thioacetamide (TAA) treatment at 3rd week in adult liver of green-fluorescent liver transgenic line and at 1st week in HCV-core protein transgenic line. Auto-activation of mature SREBP1 to SREBP1 gene in combination with C/EBP | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:24:49Z (GMT). No. of bitstreams: 1 ntu-95-R93b47112-1.pdf: 1081548 bytes, checksum: 45ac9ce32289a9a47568bac41ad90c58 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 誌謝 ………………………………………………03
Abstract ………………………………………………04 中文摘要 ………………………………………………06 序論 ………………………………………………08 材料方法 ………………………………………………12 實驗結果 ………………………………………………30 討論 ………………………………………………38 參考文獻 ………………………………………………42 圖表及圖片 ………………………………………………47 附錄 ………………………………………………67 | |
| dc.language.iso | zh-TW | |
| dc.subject | 第一型固醇調控序列結合蛋白 | zh_TW |
| dc.subject | SREBP1 | en |
| dc.title | 導致脂肪肝之斑馬魚第一型固醇調控序列結合蛋白基因被SREBP1, C/EBP apha, C/EBPbeta, 以及肝毒素thioacetamide活化 | zh_TW |
| dc.title | Activation of zebrafish SREBP1 gene by SREBP1, C/EBP apha, C/EBP beta | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳造中,李士傑 | |
| dc.subject.keyword | 第一型固醇調控序列結合蛋白, | zh_TW |
| dc.subject.keyword | SREBP1, | en |
| dc.relation.page | 72 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-29 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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