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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 常玉強(Yuh-Chyang Charng) | |
dc.contributor.author | Ching Pang | en |
dc.contributor.author | 龎靖 | zh_TW |
dc.date.accessioned | 2021-06-13T03:19:32Z | - |
dc.date.available | 2014-08-05 | |
dc.date.copyright | 2011-08-05 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-07-29 | |
dc.identifier.citation | 1. Aoyama T., Chua N.H. (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J. 11: 605-612.
2. Böhner S., Lenk I., Rieping M., Herold M., Gatz C. (1999) Transcriptional activator TGV mediates dexamethasone-inducible and tetracycline-inactivatable gene expression. Plant J. 19: 87-95. 3. Caddick M.X. (1998) An ethanol inducible gene switch for plants used to manipulate carbon metabolism. Nature Biotechnol. 16: 177-180. 4. Charng Y.C., Pfitzner A.J.P. (1994) The firefly luciferase gene as a reporter for in vivo detection of Ac transposition in tomato plants. Plant Sci. 98: 175-183. 5. Charng Y.C., Ma C., Tu J., Kuo T.T. (1997) A 200 bp constructed inducible PR-1a promoter fusion to the Ac transposase gene drives higher transposition of a Ds element than the native PR-1a promoter fusion drives. Plant Sci. 130: 73-86. 6. Charng Y.C., Pfitzner A.J.P., Pfitzner U.M., Charng C.K.F., Chen C.M., Tu J., Kuo T.T. (2000) Construction of an inducible transposon, INAc, to develop a gene tagging system in higher plants. Mol. Breed. 6: 353-367. 7. Clough S. J., Bent A. F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J.16: 735-743. 8. de Block M., Debrouwer D., Moens T. (1997) The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters. Theor. Appl. Genet. 95: 125-131. 9. de Wet J.R., Wood K.V., Helinski D.R., DeLuca M. (1985) Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc. Natl. Acad. Sci. USA 82: 7870-7873. 10. de Wet J.R., Wood K.V., DeLuca M., Helinski D.R., Subramani S. (1987) Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell. Bio. 7: 725-737. 11. Gatz C. (1997) Chemical control of gene expression. Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 48: 89-108. 12. Gatz C., Lenk I. (1998) Promoters that respond to chemical inducers. Trends Plant Sci. 3: 352-358. 13. Kang H.G., Fang Y.W., Singh K.B. (1999) A glucocorticoid-inducible transcription system causes severe growth defects in Arabidopsis and induces defense-related genes. Plant J. 20: 127-133. 14. Luo H., Lee J.Y., Hu Q., Nelson-Vasilchik K., Eitas T.K., Lickwar C., Kausch A.P., Chandlee J.M., Hodges T.K. (2006) RTS, a rice anther specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. Plant Mol. Biol. 62: 397–408. 15. Mariani C., Debeuckeleer M., Truettner J., Leemans J., Goldberg, R.B. (1990) Introduction of male sterility in plants by a chimeric ribonuclease gene. Nature 347: 737–741. 16. Martinez A., Sparks C., Hart CA., Thompson J., Jepson I. (1999) Ecdysone agonist inducible transcription in transgenic tobacco plants. Plant J. 19: 97-106. 17. McCormac A.C., Elliott M.C., Chen D.F. (1998) A simple method for the production of highly competent cells of Agrobacterium for transformation via electroporation. Mol. Biotechnol. 9: 155-159. 18. Mett V.L., Lochhead L.B., Reynolds P.H.S. (1993) Copper controllable gene expression system for whole plants. Proc. Natl. Acad. Sci. USA 90: 4567-4571. 19. Mett V.L., Podivinsky E., Tennant A.M., Lochhead L.B., Jones W.T., Reynolds P.H.S. (1996) A system for tissue-specific copper-controllable gene expression in transgenic plants: nodule-specific antisense of asparate aminotransferase-P2. Transgenic Res. 5: 105-113. 20. Moore I., Galweiler L., Grosskopf D., Schell J., Palme K. (1998) A transcription activation system for regulated gene expression in transgenic plants. Proc. Natl. Acad. Sci. USA 95: 376-381. 21. Ouwerkerk P.B.F., de Kam R.J., Hoge J.H.C., Meijer A.H. (2001) Glucocortcooid-inducible gene expression in rice. Planta 213: 370-378. 22. Pace C.N., Grimsley G.R., Barnett B.J. (1987) Purification of ribonuclease T1. Anal. Biochem. 167: 418-422. 23. Paddon, C.J., Hartley, R.W. (1986) Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase). Gene 40: 231-239. 24. Padidam M., Gore M., Lu D.L., Smirnova O. (2003) Chemical-inducible, ecdysone receptor-based gene expression system for plants. Transgenic Res. 12: 101-109. 25. Roslan H.A. (2001) Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana. Plant J. 28: 225-235. 26. Weng R.C. (2008) Using the Tet-on system to construct an inducible transposon in plant. In National Taiwan University master thesis. 27. Salter M.G., Paine J.A., Riddell K.V., Jepson I., Greenland A.J., Caddick M.X., Tomsett A.B. (1998) Characterization of the ethanol-inducible alc gene-expression system for transgenic plants. Plant J. 16: 127-132. 28. Sambrook J., Fritsch E.F., Maniatis T. (1989) Molecular Cloning: A laboratorymanual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 29. Schena M, Loyd A.M., Davis R.W. (1991) A steroid-inducible gene expression system for plant cells. Proc. Natl. Acad. Sci. USA 88: 10421-10425. 30. Sheu J.J., Yu T.S., Yu S.M. (1996) Carbohydrate starvation stimulates differential expression of rice alpha-amylase genes that is modulates through complicated transcriptional and posttranscriptional processes. J. Biol. Chem. 271: 26998-27004. 31. Zhao, X.H., Yang, J.X., Gao, J., Zhou, Q., Kuai, B.K. (2007) Analysis of probenazole-responsiveness of rice RPR1 upstream fragments. J. Plant Physiol. and Mol. Biol. 33:524-30. 32. Zuo J., Chua N.H. (2000) Chemical-inducible systems for regulated expression of plant genes. Curr. Opin. Biotechnol. 11: 146-151. 33. Zuo J., Niu Q.W., Chua N.H. (2000) An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J. 24: 265-273. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31763 | - |
dc.description.abstract | 本實驗利用Dexamethasone(DEX)誘導之pINDEX系統結合Salicylic acid(SA)誘導之PR-1a啟動子,並分別以luciferase(luc)及barnase基因為目標基因,期望建立一有效之雙重調控系統,應用於基因轉殖植物。實驗中,首先建構兩個系統共六個構築:(一)pINLUC系統:可分為受DEX誘導之單一調控系統pI2LUC、pI3LUC及受SA與DEX誘導之雙重調控系統pI4PRLUC。(二)pINbar系統:可分為受DEX誘導之單一調控系統pI2bar、pI3bar及受SA與DEX誘導之雙重調控系統pI4PRbar。以農桿菌轉殖法在菸草、阿拉伯芥(雙子葉植物)或水稻(單子葉植物)中創造出各系統之轉殖系,並利用菸草各轉殖系之葉片及癒傷組織進行誘導與檢測。單一調控系統以不同濃度的DEX誘導。pI2LUC菸草葉片經誘導並以in vivo及in vitro冷光酶詴驗後,當DEX濃度為0.1、1與10 μM時發現冷光酶有表現(相對於未誘導處理組),但冷光酶之RLU(relative light units)值並未有明顯倍數差異。而pI2bar菸草癒傷組織以DEX誘導後,並未有明顯死亡性狀出現。雙重調控系統則以不同濃度的SA及DEX組合誘導之。pI4PRLUC菸草葉片經誘導並以in vivo及in vitro冷光酶詴驗後,當SA濃度為1、3與5 mM及DEX濃度為1、10與100 μM時發現冷光酶有表現。但在未加SA只加DEX的處理也有些微冷光酶的表現。pI4PRbar菸草癒傷組織經誘導後,當SA濃度為1 mM及DEX濃度為1、10與100 μM時,癒傷組織皆有出現褐化且死亡性狀。本實驗結果證明DEX之誘導及SA與DEX之雙重誘導可有效使轉基因植物表現目標基因。但尚未確定最適當的誘導濃度條件。因此,未來研究方向為:增加不同之誘導劑濃度組合,以增強此調控系統在植物功能基因的利用性。或是選擇不同的可誘導啟動子,例如:組織專一性啟動子等,也可替換不同的目標基因,創造出不同的植物基因調控系統。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:19:32Z (GMT). No. of bitstreams: 1 ntu-100-R97621111-1.pdf: 3320323 bytes, checksum: 77840cd12a3f048044f16cfcfdf2b782 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 目錄...................................................................................................................................i
表目錄...............................................................................................................................ii 圖目錄... ..........................................................................................................................iii 附錄目錄...........................................................................................................................v 縮寫字對照表..................................................................................................................vi 中文摘要...........................................................................................................................1 英文摘要...........................................................................................................................2 前言..................................................................................................................................3 前人研究...........................................................................................................................4 實驗設計與流程...............................................................................................................9 材料與方法.....................................................................................................................11 結果................................................................................................................................29 討論................................................................................................................................45 參考文獻.........................................................................................................................48 表....................................................................................................................................52 圖....................................................................................................................................56 附錄................................................................................................................................90 | |
dc.language.iso | zh-TW | |
dc.title | 建構雙重誘導之植物基因表現系統 | zh_TW |
dc.title | Construction of a dual inducible plant gene expression system | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 杜鎮(Jenn Tu),洪傳揚(Chwan-Yang Hong) | |
dc.subject.keyword | 糖皮質激素,水楊酸,冷光酶,雙重調控系統, | zh_TW |
dc.subject.keyword | DEX,SA,GVG,pINDEX,luc,barnase, | en |
dc.relation.page | 98 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-07-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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