以紫外光及電漿方式接枝幾丁聚醣於聚己內酯薄膜之研究

Abstract

聚酯類中，例如：聚乳酸polylactide (PLA)、polyglycolide (PGA)、poly(ε-caprolactone) (PCL)，由於具備良好的生物相容性及生物可分解性，所以，不論是材料本身或其共聚合物皆已被廣泛地使用在生醫領域，但其本身之疏水性及缺乏提供細胞辨識貼附的官能基，有時會使其用途受限，因此，為增加其效能，與其他生物性分子共同混合或形成共聚合物是常採取的策略，其中又以結合親水性高分子，形成兩性高分子更吸引了研究者的注意。 在本篇研究中選擇了疏水的聚己內酯（PCL）及親水的天然高分子幾丁聚醣作為基材，有別於化學合成的方式，嘗試使用非高溫、步驟簡單的處理方式完成聚己內酯膜的表面改質，包括：紫外光輻射及輝光電漿。再以測量基材表面親疏水性、傅立葉轉換紅外線光譜及ninhydrin assay確認接合情形。並在基材表面培養細胞，探討其生物相容性。

polyesters，such as polylactide （PLA）、polyglycolide （PGA）、 poly ε-caprolactone（PCL），and their copolymers，like polylactide-co-glycolide（PLAGA），have been widely studied and extensively applied in biomedical field， since they have the good biocompatibility and biodegradability。However， because of their hydrophobicity and lack of functional groups，they need modification inevitably to make them more usable。Among the strategies， combining the hydrophilic segments and the hydrophobic polyesters to make amphiphilic copolymer attracts most attentions。 In this study，we choose PCL and chitosan as our materials，and try to use simple methods， for instance，ultraviolet radiation and plasma， to make the grafting of chitosan on PCL membrane。 The hydrophilicity of film was investigated by contact angle，the formation and the depletion of functional groups were examined by FTIR，and the changes of surface element on film were analyzed by ninhydrin assay。 Besides, the attachment and proliferation of cells on the film evidence the cytocompatiability of our material。