請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31593完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 齊肖琪(Shau-Chi Chi) | |
| dc.contributor.author | Fu-Chih Hsu | en |
| dc.contributor.author | 許富智 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:15:27Z | - |
| dc.date.available | 2010-08-01 | |
| dc.date.copyright | 2006-08-01 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-31 | |
| dc.identifier.citation | 陳莉菁。2000。石斑虹彩病毒之病原性與疫苗開發之研究。國立台灣海洋大學水產養殖研究所碩士論文。
鄭存明。2001。魚類結病毒持續性感染之研究。國立台灣大學動物學研究所碩士論文。 吳明峰。2001。石斑魚虹彩病毒之分子特性及其疫苗之開發。國立台灣大學獸醫學研究所碩士論文。 趙嘉本。2004。台灣養殖魚類感染虹彩病毒之病理學及基因研究。國立中山大學生物科學研究所博士論文。 賴書瑾。2005。台灣石斑虹彩病毒之與其他魚類虹彩病毒之初步比較。國立台灣海洋大學水產養殖研究所碩士論文。 Adolfo García-Sastre, Andrej Egorov, Demetrius Matassov, Sabine Brandt, David E. Levy, Joan E. Durbin, Peter Palese and Thomas Muster. (1998) Influenza A Virus Lacking the NS1 Gene Replicates in Interferon-Deficient Systems. Virology 252, 324-330. Ahne W., Ogawa M,. Schlotfeldt H. J. (1990) Fish viruses: transmission and pathogenicity of an icosahedral cytoplasmic deoxyribovirus isolated from sheatfish (Silurus glanis). Journal of veterinary medicine. Series B 37(3),187-190. Alonso M., Rodr´ıguez S., P´erez-Prieto SI. (1999a) Viral coinfection in salmonids: infectious pancreatic necrosis virus interferes with infectious hematopoietic necrosis virus. Archives of virology 144, 657-673. Alonso M., Rodr´ıguez S., P´erez-Prieto SI. (1999b) Nested PCR improves detection of infectious hematopoietic necrosis virus in cells coinfected with infectious pancreatic necrosis virus. Journal of virological methods 81, 1-9. Armstrong R. D., Ferguson H. W. (1989) Systemic viral disease of the chromide cichlid Etroplus maculatus. Diseases of Aquatic Organisms 7, 155-157. Azad I.S., Shekhar M.S., Thirunavukkarasu A.R., Poornima M., Kailasam M., Rajan J.J.S., Ali S.A., Abraham M. Ravichandran P. (2005) Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer: first report from India. Disease of Aquatic Organism 63, 113-118. Barker DE, MacKinnon AM, Boston L, Burt MD, Cone DK, Speare DJ, Griffiths S, Cook M, Ritchie R, Olivier G. (2002) First report of piscine nodavirus infecting wild winter flounder Pleuronectes americanus in Passamaquoddy Bay, New Brunswick, Canada. Disease of Aquatic Organism 49(2), 99-105. Bertrand Collet, Eann S. Munro, Suresh Gahlawat, Felix Acosta, Jose Garcia, Christina Roemelt, Jun Zou, Christopher J. Secombes and Anthony E. (2006) Infectious pancreatic necrosis virus suppresses type I interferon signalling in rainbow trout gonad cell line but not in Atlantic salmon macrophages. Fish & Shellfish Immunology (article in press). Breuil G., Bonami J.R., Pepin J.F., Pichot Y. (1991) Viral infection (picorna-like virus) associated with mass mortalities in hatckery-reared sea-bass(Dicentrarcbus labrax) larvae and juveniles. Aquaculture 97, 109-116. Brudeseth B. E., Castric J., Evensen O. (2002) Studies on pathogenesis following single and double infection with viral hemorrhagic septicemia virus and infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss). Veterinary pathology 39(2), 180-9. Bloch B., Gravningen K., Larsen J. L. (1991) Encephalomyelitis among turbot associated with a picornavirus-like agent. Diseases of Aquatic Organisms 10, 65-70. Bobin B., L. Berthiaume, A. Laperriere. (1984) Study of the structural proteins of lymphocystis disease virus (LDV), STRAIN Leetown NFH, grown in tissue culture. (Etude des polypeptides de structure du virus de la maladie lymphokystiue des poissons lymphocystis disease virus multiplie en culture cellulaire.) Annales de l'Institut Pasteur. Virology 135E, 67-80. Chen Y. M., Su Y. L., Lin H. Y., Yang H. L., Chen T. Y. (2005) Cloning of an orange-spotted grouper (Epinephelus coioides) Mx cDNA and characterization of its expression in response to nodavirus. Fish & Shellfish Immunology 20(1), 58-71. Chi S. C., Lo C. F., Kou G. H., Chang P. S., Peng S. E., Chen S. N. (1997) Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel) . Journal of Fish Diseases 20, 185-193. Chi S. C., Lo B. J., Lin S. C. (2001) Characterization of grouper nervous necrosis virus (GNNV). Journal of Fish Diseases 24, 3-13. Chi S. C., Shieh J. R., Lin S. J. (2003) Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Diseases of Aquatic Organisms 55(3), 221-228. Chinchar V. G., Logue O., Antao A., Chinchar G. D. (1998) Channel catfish reovirus (CRV) inhibits replication of channel catfish herpesvirus (CCV) by two distinct mechanisms: viral interference and induction of an anti-viral factor. Diseases of Aquatic Organisms 33(2), 77-85. Chao C., Yang S., Tsai H., Chen C., Chen C., Lin C. S., Hung H. (2002) A nested PCR for the detection of grouper iridovirus in Taiwan (TGIV) in cultured hybrid grouper, giant seaperch, and largemouth bass. Journal of aquatic animal health 14(2), 104-113. Chou F. H. C., Ng M. L., Ng K. L., Loo J. J., Wee J. Y. (1994) Investigation of outbreaks of a novel disease, 'Sleepy Grouper Disease', affecting the brown-spotted grouper, Epinephelus tauvina Forskal. Journal of Fish Diseases 17(4), 417-427. Chou H. Y., Hsu C. C., Peng T. Y. (1998) Isolation and characterization of a pathogenic iridovirus from cultured grouper (Epinephelus sp.) in Taiwan. Fish pathology 33, 201-206. Comps M., Trindade M., Delsert C. L. (1996) Investigation of fish encephalitis viruses (FEV) expression in marine fishes using DIG-labeled probes. Aquaculture 143, 113-121. Dasgupta R., Ghosh A., Dasmahapatra B., Guarino L. A., Kaesberg P. (1984) Primary and secondary structure of black beetle virus RNA2, the genomic messenger for BBV coat protein. Nucleic acids research 12, 7215-7223. Dasgupta R., Sgro J. Y. (1989) Nucleotide sequences of three nodavirus RNA2’s: the messengers for their coat protein precursors. Nucleic acids research 17, 7525-7526. Deng M., He J. G., Weng S. P., Zeng Z., Long Q. W. (2001) Purification and enzyme analysis of infectious spleen and kidney necrosis virus from Siniperca chuatsi. Journal of fisheries of china 25(3), 238-243. Eaton WD. (1990) Anti-viral activity in four species of salmonids following exposure to poly inosinic: cytidylic acid. Diseases of aquatic organisms 9, 193-198. Essbauer S., Ahne W. (2002) The epizootic haematopoietic necrosis virus (Iridoviridae) induces apoptosis in vitro. Journal of veterinary medicine. B, Infectious diseases and veterinary public health 49, 25-30. Fenner B. J., Thiagarajan R., Chua H. K., Kwang J. (2006a) Betanodavirus b2 is an RNA interference antagonist that facilitates intracellular viral RNA accumulation. Journal of virology 80(1), 85-94. Fenner B. J., Goh W., Kwang J. (2006b) Sequestration and protection of double-stranded RNA by the betanodavirus b2 protein. Journal of virology 80(14), 6822-33. Fujii N., Yokota S., Yokosawa N., Okabayashi T. (2004) Molecular mechanisms for suppression of interferon signal transduction pathways caused by viral infections (review). Journal of virology 54(2), 169-78. Fukuda Y., Neuyen H. D., Furuhashi M., Nakai T. (1996) Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis. Fish Pathology 31, 165-170. Furuzawa R., Okinaka Y., Nakai T. (2006) Betanodavirus infection in the freshwater model fish medaka (Oryzias latipes). The Journal of general virology 23, 2333-2339. Glazebrook J. S., Heaaman M. P., de Beer S. W. (1990) Picona-like viral particles associated with mass mortalities in larval barramundi, lates calcarifer Bloch. Journal of Fish Diseases 13, 245-249. Goorha R., Murti G., Granoff A., Tirey R. (1978) Macromolecular synthesis in cells infected by frog virus 3. Ⅷ. The nucleus is a site of frog virus 3 DNA and RNA synthesis. Virology 84, 32-50. Goorha R. (1981) Frog virus 3 requires RNA polymerase Ⅱ for its replication. Journal of Virology 37, 496-499. Goorha R. (1982) Frog virus 3 DNA replication occurs in two stages. Journal of Virology 43, 519-528. Grotmol S., Totland G. K., Kvellestad A., Fjell K., Olsen A. B. (1995) Mass mortality of larval and juveniles hatchery-reared halibut (Hippoglossus hippoglossus L.) associated with the presence of virus-like particles in vacuolated lesions in the central nervous system and retina. Bulletin of the European Association of Fish Pathologists 15(5), 176-180. Grotmol S., Totland G. K., Kryvi H. (1997) Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS). Diseases of Aquatic Organisms 29(2), 79-84. Grotmol S., Bergh O., Totland G. K. (1999) Transmission of viral encephalopathy and retinopathy (VER）to yolk-sac larvae of the Atlantic halibut Hippoglossus hippoglossus: occurrence of nodavirus in various organs and a possible route of infection. Diseases of Aquatic Organisms 36(2), 95-106. Guo Y. X., Dallmann K., Kwang J. (2003) Identification of nucleolus localization signal of betanodavirus GGNNV protein alpha. Virology 306(2), 225-35. He J. G., Wang S. P., Zeng K., Huang Z. J., Chan S. M. (2000) Systemic disease cause by an iridovirus-like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China. Journal of Fish Diseases 23, 219-222. He J. G., Deng M., Weng S. P., Li Z., Zhou S. Y., Long X. Q., Wang X. Z., Chen S. M. (2001) Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus. Virology 291, 126-139. Hedrick R. P., Groff J. M., Mcwell T. S., Wingfield W. H. (1990) An iridovirus infection of the integument of the white sturgeon (Acipenser transmontanus). Diseases of aquatic organisms 8, 39-44. Hedrick R. P., La Patra S. E., Yun S., Lauda K.A., Jones G. R., Congleton J. L., de Kindelin P. (1994) Induction of protection from infectious hematopoietic necrosis virus in rainbow trout Onchorhynchus mykiss by pre-exposure to the avirulent cutthroat trout virus (CTV). Diseases of aquatic organisms 20, 111-118. Hegde A., Teh H. C., Lam T. J., Sin Y. M. (2003) Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulate - comparative characterization and pathogenicity studies. Archives of Virology 148, 575-586. Inouye K., Yamano K., Maeno Y., Nakajima K., Matsuoka M., Wada Y., Sorimachi M. (1992) Iridovirus infection of cultured red sea bream, Pagrus major. Fish Pathology 27, 19-21. Jensen I., Albuquerque A., Sommer A. I., Robertsen B. (2002) Effect of poly I:C on the expression of Mx protein and resistance against infectious salmon anemia virus in Atlantic salmon. Fish & shellfish immunology 13(5), 311-326. Johnson S. C., Groman D. B., Cusack R. R., Sperker S. A., Leggiadro C. T., Ritchie R., Cook M. D. (2001) Identification and characterization of a piscine neuropathy nodavirus from juvenile Altantic cod (Gadus morbua).Abtract P-045. In: Tenth International Conference of the European Association of Fish Pathologists, 9-14. Kaesberg P., Johnson J. E. (1990) Structural homology among four nodaviruses as dedused by sequencing and X-ray crystallography. Journal of molecular biology 214, 423-435. Kasornchandra, J., Khongoradit R. (1997) Isolation and preliminary characterization of a pathogenic iridovirus in Nursing grouper, Epinephilus inalabaricus. Diseases of Asian aquaculture Ⅲ, 61-66. Langdon J. S., Humphrey J. D., Williams L. M. (1986) First virus isolation from Austraoian fish: an iridovirus-like pathogen from redfin perch, Perca fluviatilis. Journal of fish diseases 9, 263-268. Langdon J. S., Humphrey J. D., Williams L. M. (1988) Outbreaks of an EHNV-like iridovirus in cultured rainbow trout, Salmo gairdneri Richardson, in Australia. Journal of fish diseases 11, 93-96. LaPatra S. E., Groff J. M., Jones G. R., Munn B., Patterson T. L., Holt R. A., Hauck A. K., Hedrick R. P. (1994) Occurrence of white sturgeon iridovirus infection among cultured white sturgeon in the Pacific Northwest. Aquaculture 126, 201-210. Lee K. W., Chi S. C., Cheng T. M. (2002) Interference of the life cycle of fish nodavirus with fish retrovirus. The Journal of general virology 83(10), 2469-74. Liu W., Hsu C. H., Hong Y. R., Wu S. C., Wang C. H., Wu Y. M., Chao C. B., Lin C. S. (2005) Early endocytosis pathways in SSN-1 cells infected by dragon grouper nervous necrosis virus. The Journal of general virology 86(9), 2553-61. Lu L., Zhou S. Y., Chen C., Weng S. P., Chan S. M., He J. G. (2005) Complete genome sequence analysis of an iridovirus isolated from the orange-spotted grouper, Epinephelus coioides. Virology 339(1), 81-100. Mao J., Hedrick R. P., Chinchar V. G. (1997) Molecular characterization, sequence analysis, and taxonomic position of newly isolated fish iridoviruses. Virology 229, 212-220. Mori K., Nakai T., Negahara M., Muroga K., Mckuchi T., Kanno T. (1991) A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Fish Pathology 26, 209-210. Mori K., Nakai T., Muroga K., Arimoto M., Mushiake K., Furusawa I. (1992) Properties of a new virus belonging to nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis. Virology 187, 368-371. Mori K., Mangyoku T., Iwamoto T., Arimoto M., Tanaka S., Nakai T. (2003) Serological relationships among genotypic variants of betanodavirus. Diseases of aquatic organisms 57,19-26. Muroga K. (1995) Viral and bacterial disease in larval and juvenile marine fish and shellfish: a review. Fish Pathology 30, 71-85. Munday B. L., Langdon J. S., Hyatt A., Humphrey J. D. (1992) Mass mortality associated with a viral-induced vacuolating encephalopathy and retinopathy of larval and juvenile barramundi, Lates calcarfer Bloch. Aquaculture 103, 197-211. Munday B. L., Nakai T. (1997) Special topic review: Nodaviruses as pathogens in larval and juvenile marine finfish. World Journal of microbiology & Biotechnoogy 13, 375-381. Munday B. L., Kwang J., Moody N. (2002) Betanodavirus infectious of teleost fish: a review. Journal of Fish Diseases 25, 127-142. Nakai T., Nguyen H.D., Nishizawa T., Muroga K., Arimoto M., Ootsuki K. (1994) Occurrence of viral nervous necrosis in kelp grouper and tiger puffer. Fish Pathology 29, 211-214. Nakajima K., Sorimachi M. (1994) Biological and physico-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major. Fish Pathology 29, 29-33. Nguyen H. D., Mekuchi T., Imura K., Nakai T., Nishizawa T., Muroga K. (1994) Occurrence of viral nervous necrosis (VNN) in hatchery-reared juvenile Japanese flounder Paralichthys olivaceus. Fisheries Science 60, 551-554. Nguyen H. D., Nakai T., Muroga K. (1996) Progression of striped jack nervous necrosis virus (SJNNV) in adult striped jack Pseudocaranx dentex larvae. Diseases of Aquatic Organisms 24(2), 99-105. Nishizawa T., Mori K., Arimoto M., Nakai T. (1994) Polymerase chain reation (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Disease of Aquatic Organisms 18, 103-107. Nishizawa T., Mori K., Furuhashi M., Nakai T., Furusawa I., Muroga K. (1995) Comparison of the coat protein genes of five fish nodaviruses, the causative agent of nervous necrosis in marine fish. Journal of General Virology 76(7), 1563-1569. Nishizawa T., Furuhashi M., Nagal T., Muroga K. (1997) Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Applied and environmental microbiology 63, 1633-1636. Nygaard R., Husgard S., Sommer A. I., Leong J. C., Robertsen B. (2000) Induction of Mx protein by interferon and double-stranded RNA in salmonid cells. Fish & shellfish immunology 10(5), 435–450. OIE (2000) Chapter 2.2.2. Viral encephalopathy and retinopathy. In: OIE Diagnostic Manual for Aquatic Animal Diseases, 3rd edn: 69-73. Pakingking R. J., Okinaka Y., Mori K., Arimoto M., Muroga K., Nakai T. (2004) In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus. Fish & shellfish immunology 17(1), 1-11. Pakingking R. J., Mori K. I., Sugaya T., Oka M., Okinaka Y., Nakai T. (2005) Aquabirnavirus-induced protection of marine fish against piscine nodavirus infection. Fish Pathology 40, 125-131. Qin Q. W., Shi C., Gin K. Y., Lam T. J. (2002) Antigenic characterization of a marine fish iridovirus from grouper, Epinephelus spp. Journal of virological methods 106(1), 89-96. Remenick J., Kenny M. K., McGowan J. J. (1988) Inhibition of adenovirus DNA replication by vesicular stomatitis virus leader RNA. Journal of virology 62(4), 1286-92. Renault T., Haffner P. H., Baudin L. F., Breuil G., Bonami J. R. (1991) Mass mortalities in hatchery-reared sea bass (Lates calcarifer) larvae associated with the presence in the brain and retina of virus-like particles. Bulletin of the European Association of Fish Pathologists 11, 68-73. Robertsen B., Trobridge G., Leong J. (1997) Molecular cloning of the double-stranded RNA inducible Mx genes of Atlantic salmon (Salmo salar L.). Developmental and comparative immunology 21, 397-412. Robertsen B. (1998) Immunology of fishes. Cytokine. In：Pastoret, P.-P.,Griebel, P.,Bazin, H., Goaverts, A.,(eds.), Handbook of Vertebrate Immunology, 11-13. Rodger H. D., Kobs M., Macartney A., Frerichs G. N. (1997) Systemic iridovirus infection in freshwater angelfish, Pterophyllum scalare (Lichtenstein). Journal of Fish Diseases 20, 69-72. Rodriguez S., Alonso M., Perez-Prietol SI. (2005) Comparison of two birnavirus-rhabdovirus coinfections in fish cell lines. Disease of Aquatic Organisms 67(3), 183-90. Rogel-Gaillard C., Chilmonczyk S., De Kinkelin P. (1993) In vitro induction of interferon-like activity from rainbow trout leucocytes stimulated by Egtved virus. Fish & shellfish immunology 3(5), 383-394. Samuel C. E., Antiviral actions of interferon. (1991) Interferon-regulated cellular proteins and their surprisingly selective antiviral activities. Virology 183, 1–11. Schnitzler P., Darai G. (1993) Identification of the gene encoding the major capsid protein of fish lymphocystis disease virus. Journal of General Virology 74(10), 2143-2150. Siwicki A. K., Pozet F., Morand M., Volatier C., Terech-Majewska E. (1999) Effects of iridovirus-like agent on the cell-mediated immunity in sheatfish (Silurus glanis) - an in vitro study. Virus Research 63, 115-119. Sommerset I., Nerland A. H. (2004) Complete sequence of RNA1 and subgenomic RNA3 of Atlantic halibut nodavirus (AHNV). Diseases of aquatic organisms 58(2-3), 117-125. Staeheli P., Yu Y. X., Grob R., Haller O. (1989) A double-stranded RNA-inducible fish genes homologous. Molecular and cellular biology 9(7), 3117–3121. Starkey W. G., Ireland J. H., Muir K. F., Jenkins M. E, Roy W. J., Richards R. H., Ferguson H.W. (2000) Nodavirus infection in Altantic cod, Dover sole and halibut in the United Kingdom. Abstract P-116, In: Tenth International Conference of the Eurpean Association of Fish Pathologists, 9-14. Stohwasser R., Raab K., Schnitzler P., Janssen W., Darai G. (1993) Identification of the gene encoding the major capsid protein of insect iridescent virus type 6 by polymerase chain reaction. Journal of General Virology 74(5), 873-879. Sudthongkong C., Miyata M., Miyazaki T. (2002a) Iridovirus disease in two ornamental tropical freshwater fish: African lampeye and dwarf gourami. Diseases of aquatic organisms 48(3), 163-173. Sudthongkong C., Miyata M., Miyazaki T. (2002b) Viral DNA sequences of genes encoding the ATPase and the major capsid protein of tropical iridovirus isolates which are pathogenic to fishes in Japan, south China sea and southeast Asian countries. Archives of Virology 147(11), 2089-2109. Ting J. W., Wu M. F., Tsai C. T., Lin C. C., Guo L. C., Chang C. Y. (2004) Identification and characterization of novel gene of grouper iridovirus encoding a purine nucleoside phosphorylase. Journal of General Virology 85(10), 2883-2892. Tsai C. T., Ting J. W., Wu M. H., Wu M. F., Guo I. C., Chang C. Y. (2005) Complete genome sequence of the grouper iridovirus and comparison of genomic organization with those of other iridoviruses. Journal of Virology 79(4), 2010-23. Webby R. J., Kalmakoff J. (1999) Comparison of the major capsid protein genes, terminal redundancies, and DNA-DNA homologies of two New Zealand iridovirus. Virus research 59, 179-189. Willis D. B., Gooha R., Granoff A. (1984) DNA methytransferase induced by frog virus 3. Journal of Virology 49, 86-91. Wolf K., Vestergard-Jorgensen PE. (1970) Salmonid viruses: double infection of RTG-2 cells with Egtved and infectious pancreatic necrosis viruses. Archiv für die gesamte Virusforschung 29(4), 337-42. Worf K., C. P. Carlson. (1965) Multiplication of lymphocystis virus in the bluegill(Lepomis macrochirus). Annals of the New York Academy of Sciences 126, 414-419. Yoshikoshi K., Inoue K. (1990) Viral nervous necrosis in hatchery-reared larvae and juveniles of Japanese parrotfish, Oplegnathus fasciatus (Temminck＆Schlegel). Journal of Fish Diseases 13, 69-77. Yuan C. H., Chen H. R. (2006) Identification of the factors associated with the coat protein of nervous necrosis virus. The Chinese Sociely of Cell and Molecular Biology (Fourteenth Symposium on Recent Advances in Cellular and Molecular Biology). | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31593 | - |
| dc.description.abstract | 神經壞死症病毒(Nervous necrosis virus, NNV)及台灣石斑魚虹彩病毒(Grouper iridovirus of Taiwan, TGIV)是造成養殖石斑苗大量死亡的病因，NNV造成兩吋以下的魚苗大量死亡，而TGIV則造成一吋以上的魚苗陸續死亡，在養殖石斑魚的一至兩吋苗階段，是兩種病毒容易有共感染的階段。本研究目的在探討NNV及TGIV在同時感染或先後感染時NNV對TGIV複製的影響。活體攻毒實驗結果顯示, 以TGIV單獨感染健康的吋苗，10天後的累計死亡率是100%；然而以NNV先攻毒健康的八分苗，15天後的殘活吋苗再進行TGIV攻毒，則10天後的累計死亡率降至28%。以石斑魚鰭細胞株GF-3 當作體外培養系統，在NNV和TGIV同時感染或NNV先感染的情況下，TGIV的力價會比單獨感染時的力價低2~2.25 Log，但NNV力價卻比NNV單獨感染時力價上升0.25~1.25 Log。但單獨感染NNV的GF-3細胞中可以偵測到Mx基因的表現，在單獨感染TGIV的GF-3細胞中則測不到，顯示只有NNV可以誘發GF-3細胞產生干擾素(Interferon, IFN)反應；抗病毒活性試驗結果顯示，NNV所誘發的干擾素對NNV及TGIV都有抗病毒效果，推測NNV所誘發的干擾素反應可能是造成先後感染(dual-infection)實驗中TGIV力價降低的原因之一。另外，TGIV 是DNA 病毒，NNV是正意單股RNA病毒，同時感染及先後感染實驗結果顯示，TGIV的DNA、RNA及蛋白質複製量都比單獨感染時明顯下降，NNV的RNA複製量則比單獨感染時少許上升，推測造成此現象的原因是NNV的核酸比TGIV小很多，故複製效率因此比TGIV快很多，可能因快速耗損寄主細胞的資源，而造成TGIV力價下降。 | zh_TW |
| dc.description.abstract | Nervous necrosis virus (NNV) and grouper iridovirus of Taiwan (TGIV) were two important pathogens of reared groupers. Serious mortality caused by NNV usually occurs among the groupers smaller than 2 inches, and mortality caused by TGIV always occurs among groupers larger than one inch. In nature, NNV and TGIV had been detected within the same host. The aim of this study is to study the interaction between NNV and TGIV during co-infection and dual-infection.The accumulated mortality of grouper larvae with body length of one inch 10 days post TGIV challenge was 100%. If NNV challenged grouper larvae with body size of 0.8 cm first, then TGIV challenged the survivor larvae 15 days post NNV infection, with body length of 4~4.5cm the accumulated mortality of TGIV-infected groupers declined to 28%. Grouper fin cell line GF-3 is susceptible to either NNV or TGIV. During co-infection or dual-infection of NNV and TGIV in GF-3 cells, the titers of progeny TGIV decreased 2-2.25 Log, but the titers of NNV increased 0.25-1.25 Log. In GF-3 cells, only NNV but not TGIV could induce Mx gene expression. The culture supernatant of NNV-infected GF-3 cells being neutralized by NNV-specific antibodies revealed antiviral activity against both viruses.Therefore, NNV infection induced interferon (IFN) response may be one of the reasons for the decline of TGIV replication during co- or dual- infection.Moreover, the levels of DNA, RNA and protein synthesis of TGIV in GF-3 cells during co- or dual-infection with NNV were much lower than the levels during TGIV sole infection.However, the replication efficiency of NNV during co- or durl-infection was similar to that during NNV sole infection. NNV is a positive sence ssRNA virus, and the genome size is much smaller than that of TGIV which is a dsDNA virus.The decline of TGIV replication level during co- or dual-infection was also possible due to the rapid comsumption of host resources by NNV. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:15:27Z (GMT). No. of bitstreams: 1 ntu-95-R93b41012-1.pdf: 643296 bytes, checksum: cf80b3d02cc73e8da513d8a449719457 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 中文摘要........................................................................................ Ⅰ
英文摘要......................................................................................... Ⅱ 目錄................................................................................................. Ⅳ 圖目次................................................................................................ Ⅵ 表目次................................................................................................ Ⅶ 第一章 緒論..................................................................................... 1 第二章 材料與方法.......................................................................... 13 1. 病毒與細胞........................................................................... 13 2. 病毒製備............................................................................... 13 3. 病毒力價測定....................................................................... 14 4. 核酸的萃取........................................................................... 14 5. 反轉錄-聚合酶連鎖反應........................................................ 15 6. 及時定量聚合酶連鎖反應(Real-Time Quantitative PCR)...... 16 7. NNV與TGIV共感染石斑魚苗攻毒試驗................................. 17 8. NNV與TGIV共感染GF-3細胞株之力價測定........................ 18 9. 病毒感染細胞株之病毒力價日變化....................................... 18 10. 共感染後之病毒液連續繼代之病毒力價................................ 19 11. 病毒感染GF-3細胞與Mx基因的表現................................... 19 12. 比較TGIV單獨感染與共感染時病毒蛋白質的表現量............ 19 13. 比較NNV與TGIV共感染時，TGIV病毒核酸的複製量.......... 20 14. 抗病毒活性測試.................................................................... 21 第三章 結果....................................................................................... 22 一、 NNV及TGIV共感染石斑魚苗之病程及累計死亡率............... 22 二、 比較NNV及TGIV單獨、同時及先後感染之病毒力價變化...... 22 三、比較NNV和TGIV同時感染及先後感染時病毒核酸與蛋白質 複製量................................................................................ 23 四、 NNV與TGIV單獨感染GF-3細胞，病毒核酸的時序變化..... 23 五、 NNV及TGIV單獨感染GF-3細胞，病毒力價的日變化........ 23 六、 病毒共感染後子代病毒連續繼代的力價的變化..................... 24 七、 NNV及TGIV對誘發寄主細胞產生干擾素反應之測試.......... 24 八、 NNV誘發GF-3細胞產生的干擾素之抗病毒活性................. 25 第四章 討論................................................................................. 26 一、 NNV和TGIV共感染石斑魚苗降低TGIV對石斑魚的致死 率.............................................................................. 26 二、NNV和TGIV共感染GF-3細胞會干擾TGIV的複製量........ 26 1. NNV在GF-3細胞中的大量複製遠早於TGIV的複製….. 27 2. 干擾素的誘發與抗病毒活性........................................... 28 3. 病毒與病毒之間的交互作用........................................... 29 三、未來實驗及展望..................................................................... 31 第五章 參考文獻............................................................................ 33 圖與表….......................................................................................... 51 附錄................................................................................................ 63 | |
| dc.language.iso | zh-TW | |
| dc.subject | 同時感染 | zh_TW |
| dc.subject | 石斑魚虹彩病毒 | zh_TW |
| dc.subject | 石斑魚神經壞死症病毒 | zh_TW |
| dc.subject | 先後感染 | zh_TW |
| dc.subject | co-infection | en |
| dc.subject | grouper iridovirus | en |
| dc.subject | grouper nervous necrosis virus | en |
| dc.subject | dual-infection | en |
| dc.title | 石斑魚神經壞死症病毒對石斑魚虹彩病毒在共感染及先後感染期間的干擾效應 | zh_TW |
| dc.title | Interference of the replication of grouper iridovirus by grouper nervous necrosis virus during co- and dual- infection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅竹芳(Chu-Fang Lo),周信佑,王金和 | |
| dc.subject.keyword | 石斑魚神經壞死症病毒,石斑魚虹彩病毒,同時感染,先後感染, | zh_TW |
| dc.subject.keyword | grouper nervous necrosis virus,grouper iridovirus,co-infection,dual-infection, | en |
| dc.relation.page | 64 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-31 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-95-1.pdf 未授權公開取用 | 628.22 kB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。