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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 盧虎生 | |
dc.contributor.author | Yu-Hang Lu | en |
dc.contributor.author | 陸禹涵 | zh_TW |
dc.date.accessioned | 2021-06-13T03:12:40Z | - |
dc.date.available | 2014-08-23 | |
dc.date.copyright | 2011-08-23 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-20 | |
dc.identifier.citation | Ampomah-Dwamena C, Morris BA, Sutherland P, Veit B, Yao JL (2002) Down-regulation of TM29, a tomato SEPALLATA homolog, causes parthenocarpic fruit development and floral reversion. Plant Physiol 130: 605-617
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Plant Cell 16: S1-S17 Kang BC, Yeam I, Jahn MM (2005) Genetics of plant virus resistance. Annu Rev Phytopathol 43: 581-621 Kotilainen M, Elomaa P, Uimari A, Albert VA, Yu DY, Teeri TH (2000) GRCD1, an AGL2-like MADS box gene, participates in the C function during stamen development in Gerbera hybrida. Plant Cell 12: 1893-1902 Ma H (1994) The unfolding drama of flower development - recent results from genetic and molecular analyses. Gene Dev 8: 745-756 Ma H, dePamphilis C (2000) The ABCs of floral evolution. Cell 101: 5-8 Mandel MA, Yanofsky MF (1998) The Arabidopsis AGL9 MADS box gene is expressed in young flower primordia. Sex Plant Reprod 11: 22-28 Pelaz S, Ditta GS, Baumann E, Wisman E, Yanofsky MF (2000) B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405: 200-203 Pelaz S, Gustafson-Brown C, Kohalmi SE, Crosby WL, Yanofsky MF (2001a) APETALA1 and SEPALLATA3 interact to promote flower development. Plant J 26: 385-394 Pelaz S, Tapia-Lopez R, Alvarez-Buylla ER, Yanofsky MF (2001b) Conversion of leaves into petals in Arabidopsis. Curr Biol 11: 182-184 Pnueli L, Hareven D, Broday L, Hurwitz C, Lifschitz E (1994) The TM5 mads box gene mediates organ differentiation in the 3 inner whorls of tomato flowers. Plant Cell 6: 175-186 Ratcliff FG, MacFarlane SA, Baulcombe DC (1999) Gene silencing without DNA. RNA-mediated cross-protection between viruses. Plant Cell 11: 1207-1216 Riechmann JL, Meyerowitz EM (1997) Determination of floral organ identity by Arabidopsis MADS domain homeotic proteins AP1, AP3, PI, and AG is independent of their DNA-binding specificity. Mol Biol Cell 8: 1243-1259 Ruokolainen S, Ng YP, Albert VA, Elomaa P, Teeri TH (2010a) Large scale interaction analysis predicts that the Gerbera hybrida floral E function is provided both by general and specialized proteins. BMC Plant Biol 10: 129 Ruokolainen S, Ng YP, Broholm SK, Albert VA, Elomaa P, Teeri TH (2010b) Characterization of SQUAMOSA-like genes in Gerbera hybrida, including one involved in reproductive transition. BMC Plant Biol 10: 128 Savidge B, Rounsley SD, Yanofsky MF (1995) Temporal relationship between the transcription of two Arabidopsis MADS box genes and the floral organ identity genes. Plant Cell 7: 721-733 Theissen G, Kim JT, Saedler H (1996) Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene subfamilies in the morphological evolution of eukaryotes. J Mol Evol 43: 484-516 Theissen G, Saedler H (2001) Plant biology. Floral quartets. Nature 409: 469-471 Uimari A, Kotilainen M, Elomaa P, Yu D, Albert VA, Teeri TH (2004) Integration of reproductive meristem fates by a SEPALLATA-like MADS-box gene. Proc Natl Acad Sci U S A 101: 15817-15822 Weigel D, Meyerowitz EM (1994) The ABCs of floral homeotic genes. Cell 78: 203-209 Yang YZ, Fanning L, Jack T (2003) The K domain mediates heterodimerization of the Arabidopsis floral organ identity proteins, APETALA3 and PISTILLATA. Plant J 33: 47-59 Zahn LM, King HZ, Leebens-Mack JH, Kim S, Soltis PS, Landherr LL, Soltis DE, dePamphilis CW, Ma H (2005) The evolution of the SEPALLATA subfamily of MADS-Box genes: A preangiosperm origin with multiple duplications throughout angiosperm history. Genetics 169: 2209-2223 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31407 | - |
dc.description.abstract | 近年來ABCE模型已廣泛的解釋不同物種中花朵器官的形成,而花朵器官的發育乃由A-class (AP1及AP2)、B-class (AP3及PI)、C-class (AG)和E-class (SEP)四群基因的交互作用所調控。而E-class基因在阿拉伯芥當中擁有功能上的保守性。單一SEP基因的突變並不會造成外表型的改變,而阿拉伯芥的四個SEP中,在有三個或四個基因發生突變時,則分別使所有花朵器官轉變為花萼和葉片。在矮牽牛當中目前已發現E-class基因有FBP2、FBP4、FBP5、FBP9、FBP23與MADS12六個,但是僅僅是FBP2與FBP5的突變即可在花瓣、雄蕊及心皮中觀察到花朵器官的改變。並且從序列的分類來看,矮牽牛中具有一只在茄科植物中存在的次類群(FBP9和FBP23)。因此懷疑在矮牽牛中的E-class基因已有不同的特化與分工。為了瞭解矮牽牛中E-class基因的功能保守性是否與阿拉伯芥的E-class基因有所不同,本研究觀察擁有葉化萼片的實驗品種M1與擁有正常花萼之實驗品種W115做比較,分析其花朵四輪構造中E-class基因表現量的差異;以及使用利用Tobacco Rattle Virus (TRV) 誘發之Virus-Induced Gene Silencing (VIGS) 的技術,利用FBP23的保守性片段、部分保守性片段與專一性片段,靜默矮牽牛當中E-class的基因,觀察花朵外表型的改變與E-class基因RNA表現量間的關係。結果顯示M1萼片中除了MADS12外的E-class基因表現量很低。在接種三種含FBP23片段之TRV誘發VIGS之花朵的結果顯示,單獨靜默FBP23並沒有明顯的外表型,而在接種含FBP9和FBP23專一片段的TRV植株中,觀察到FBP9表現量提升,是否與萼片葉化有關目前還無定論。變異的花朵中,瓣化雄蕊的FBP2、FBP9與MADS12其表現量與正常花瓣類似,相較於M1中花瓣與雄蕊的差異,可能為FBP4、FBP5與FBP23共同的調控雄蕊與花瓣的決定。 | zh_TW |
dc.description.abstract | ABCE model has been widely interpreted in flower organ formation of different species. In recent years, According to the model, flower organ formation is determined by interaction of four groups of genes, A-class (AP1 and AP2), B-class (AP3 and PI), C-class (AG) and E- class (SEP) genes. In Arabidopsis, there is a conservative function in E-class proteins. No phenotypic change was observed in single SEP gene mutants while when tree or four Arabidopsis SEP genes were mutated, all flowers organs were converted into sepals and leaves respectively. In Petunia, six E-class proteins, FBP2, FBP4, FBP5, FBP9, FBP23 and MADS12 have been found, and it was reported that down-regulation of two genes, FBP2 and FBP5, petals, stamens, and carpels were converted into sepaloid organs. The phylogenetic tree indicated that one subgroup of E-class genes (FBP9 and FBP23) only existed in Solanaceae. It was suspected that there are specialization and different functions in the E-class genes of petunia. To find out the functional differences among Petunia E-class proteins, we compared transcript abundance of E-class genes of experiment strain M1, which has leafloid sepals, and W115, which has normal sepals. Moreover, we used Tobacco Rattle Virus (TRV) that contains a FBP23 cDNA fragment to inoculate petunia plants to induce gene silencing, and then analyzed the correlation between morphological changes of flower organs and transcript abundance of E-class genes. The results showed that most of the E-class genes were repressed expect MADS12 in sepals of M1 flowers. Flowers from plants inoculated with TRV containing specific FBP23 sequence fragment showed no obvious morphological changes. However, we observed that an increase of FBP9 expression and an reduction of FBP23 expression in a flower showed leafloid sepals, which was from a plant inoculated with TRV containing FBP9 and FBP23 specific sequence fragments. In transformed flower, the expression pattern of FBP2, FBP9 and MADS12 in petaloid stamen is similar to normal petals. Compare with the petals and stamen of M1, that FBP4, FBP5 and FBP23 may be the co-regulators of petals and stamen identity. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:12:40Z (GMT). No. of bitstreams: 1 ntu-100-R97621113-1.pdf: 2351379 bytes, checksum: fdab01faef564bb34e0a03d6f2a5b646 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 論文口試委員會審定書 i
謝誌 ii 中文摘要 iii ABSTRACT iv 壹、前人研究 1 花朵發育與MADS基因群 1 ABC模型 2 E-class基因 3 E-class蛋白具高度功能保守性 4 E-class蛋白質與A, B, C-class 蛋白形成高分子聚合的四級結構 4 E-class基因的分群 5 不同物種上的E-class基因 6 矮牽牛上的E-class基因 6 病毒誘導基因靜默 (Virus-induced gene silencing: VIGS) 7 研究方向 9 貳、材料與方法 10 1. 植物材料及栽培環境 10 2. 組織透明法 10 3. 目標基因之釣取 (target gene cloning) 11 4. TRV表現載體pTRV1 (pYL192)及pTRV2 (pYL156) 11 5. 製備含有CHSA (chalcone synthase A)片段CHSA之載體 12 5.1擴增CHSA片段與純化 12 5.2純化之CHSA片段酶切 12 5.3 載體製備 13 5.3.1 鹼性磷酸酶 (Alkaline Phosohatase (CIAP))處理: 14 5.3.2 黏合作用 (ligation) 14 5.3.3 大腸桿菌 (E.coli)之轉型作用 (transformation) 14 5.3.4純化經酶切之載體 15 6. 製備含有FBP23片段之FBP23S、FBP23P和FBP23C片段之載體 15 6.1 擴增FBP23S、FBP23P和FBP23C片段與純化 15 6.2 純化之FBP23S、FBP23P和FBP23C片段酶切 17 7. 製備同時含有FBP23和FBP9片段之FBP23+9載體 18 8. VIGS植株接種製備 19 8.1 製備轉型作用之質體 19 8.2 電穿孔法 (electroporation)-農桿菌 (Agrobacterium)之轉型作用 (transformation) 20 8.3 接種 (inoculation)植株 20 9. 採取植株樣本與分析 21 9.1 RNA萃取 21 9.2 去氧核糖核酸水解酶處理 (DNase treatment) 22 9.3 反轉錄聚合酵素鏈鎖反應 (Reverse transcription) 22 10. 聚合酵素鏈鎖反應 (PCR) 22 10.1 2X Taq DNA polymerase Mastermix-RED (Bioman) 22 10.2 2X Taq PCR MasterMix (Genomics) 23 10.3 TaKaRa Ex Taq™ 23 11.膠體、DNA之純化 23 12.統計 24 參、結果 25 M1與W115花朵型態之差異 25 M1與W115花朵四輪SEPALLATA基因表現量之差異 25 插入TRV之FBP23片段與其他矮牽牛基因的保守性分析 26 接種帶有FBP23片段之VIGS載體之植株外表型觀察與分類 27 分析六種型態花朵其SEPALLATA基因群之表現 28 接種不同FBP23片段植株間的差異 28 接種FBP23片段植株變異花朵四輪個別SEPALLATA基因表現分析 29 肆、討論 30 葉化花萼與FBP9的關聯 31 M1與W115花瓣中SEPALLATA基因功能的保守性 31 花瓣與瓣化雄蕊間的關係 32 在M1與W115雄蕊中FBP5與FBP9的差異 32 VIGS產生心皮轉變為新生花序對於RNA表現量的影響 32 接種含有FBP23不同區段之TRV在基因表現量的差異 33 花朵變異程度與SEPALLATA基因表現量的關係 33 伍、參考文獻 35 陸、圖表 39 | |
dc.language.iso | zh-TW | |
dc.title | 靜默矮牽牛中SEPALLATA基因造成花朵器官的轉變 | zh_TW |
dc.title | Effects of floral organ conversions caused by silencing of petunia SEPALLATA | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳仁治 | |
dc.contributor.oralexamcommittee | 王俊能(Chun-Neng Wang),張松彬 | |
dc.subject.keyword | 矮牽牛,VIGS,SEPALLATA,FBP9,FBP23,萼片葉化, | zh_TW |
dc.subject.keyword | petunia,VIGS,SEPALLATA,FBP9,FBP23,leafoid sepal, | en |
dc.relation.page | 54 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-21 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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