松杉靈芝對過敏免疫反應之調節研究

Miaw-Ling Chen; 陳妙齡

請用此 Handle URI 來引用此文件： http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31345

完整後設資料紀錄

DC 欄位	值	語言
dc.contributor.advisor	林璧鳳(Bi-Fong Lin)
dc.contributor.author	Miaw-Ling Chen	en
dc.contributor.author	陳妙齡	zh_TW
dc.date.accessioned	2021-06-13T02:44:25Z	-
dc.date.available	2011-10-31
dc.date.copyright	2006-10-31
dc.date.issued	2006
dc.date.submitted	2006-10-26
dc.identifier.citation	牟宗宜 (1998) 炸油與維生素E對卵蛋白致敏BALB/c鼠過敏免疫反應之影響。台大農化所碩士論文。呂丹妮、余淑絹、曾哲明 (1994) 茯苓對人體血液淋巴球分泌免疫球蛋白的影響。師大生物學報。29:43-51。李佩芸 (2005) 口服 Dp2 重組蛋白質對致敏小鼠過敏免疫調節之影響。台大微生物與生化學所碩士論文。李旭生、廖英明、劉玲、魏耀揮、陳光耀 (1987a) 靈芝菌絲體與子實體萃取物抗腫瘤效應之比較。中華癌症會刊。3：38。李旭生、張琳、柯立文、陳介甫 (1987b) 細胞培養分析法探討靈芝抗癌成分及作用之研究。中華癌症會刊。3：38-39。林金源 (2001) 台灣產野生刺莧水萃取物對免疫功能的影響。台大農化所博士論文。洪永瀚、林璧鳳 (2004) 稻苗草榨汁對抑制發炎反應的功能研究。台灣農業化學與食品科學。42：456-465。許瑞祥 (1993) 靈芝概論。萬年出版社。台中市。程志恒 (1997) n-3多元不飽和脂肪酸對致敏BALB/c鼠過敏免疫反應之影響。台大農化所碩士論文。陳妙齡 (2000) 以松杉靈芝餵食BALB/c鼠探討腹腔免疫反應的功能評估指標。台大農化所碩士論文。郭明德、王文音、董大成 (1982) 人工液體培養之靈芝萃取物抗老鼠S-180肉瘤生長的作用。中華民國癌醫會刊。3：35-40。黃繼萱 (2006) 以動物模式評估液態靈芝產品對免疫功能的調節。台大微生物與生化學研究所碩士論文。賴怡琪、劉倩君、曾哲明 (1993) 中藥茯苓對老鼠B淋巴球分泌免疫球蛋白的影響。師大生物學報。28:53-63。鄭曉倩 (2001) 炸油對卵蛋白致敏BALB/c鼠產生過敏反應的影響機制探討。台大農化所碩士論文。謝貴雄 (1995) 小兒氣喘病之預防與處理。小兒過敏病叢書之九。中華民國過敏及氣喘病研究協會印製。羅文音 (2006) 以卵白蛋白致敏小鼠模式探討攝食山藥對過敏性氣喘的影響。台大微生物與生化學研究所碩士論文。蕭慧美、夏彩蘭 (2006) 餵食靈芝菌絲體對小鼠體內抗氧化能力之影響。中華民國營養學會雜誌。31: 17-23。 Adcock, I. M., Lane, S. J., Brown, C. R., Lee, T. H. and Barnes, P. J. (1995) Abnormal glucocorticoid receptor-activator protein 1 interaction in steroid-resistant asthma. J. Exp. Med. 182: 1951-1958. Ahn, K. S. and Aggarwal, B. B (2005) Transcription factor NF-{kappa}B: A sensor for smoke and stress signals. Ann N Y Acad Sci. 1056: 218-233. Ahn, W. S., Kim, D. J., Chae, G. T., Lee, J. M., Bae, S. M., Sin, J. I., Kim, Y. W., Namkoong, S. E. and Lee, I. P. (2004) Natural killer cell activity and quality of life were improved by consumption of a mushroom extract, Agaricus blazei Murill Kyowa, in gynecological cancer patients undergoing chemotherapy. Int. J. Gynecol. Cancer. 14: 589-594. Akdis, M., Verhagen, J., Taylor, A., Karamloo, F., Karagiannidis, C., Crameri, R., Thunberg, S., Deniz, G., Valenta, R., Fiebig, H., Kegel, C., Disch, R., Schmidt-Weber, C. B., Blaser, K. and Akdis, C. A. (2004) Immune responses in health and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J. Exp. Med. 199: 1567-1575. Akihisa, T., Tagata, M., Ukiya, M., Tokuda, H., Suzuki, T. and Kimura, Y. (2005) Oxygenated lanostane-type triterpenoids from the fungus Ganoderma lucidum. J. Nat. Prod. 68: 559-563. Albers, R., Bol, M., Bleumink, R., Willems, A., Blonk, C. and Pieters, R. (2002) Effects of dietary lipids on immune function in a murine sensitization model. Br. J. Nutr. 88: 291-299. Angus, R. M., Millar, E. A., Chalmers, G. W. and Thormson, N. C. (1995) Effect of inhaled atrial natriuretic peptide and a neutral endopeptidase inhibitor on histamine-induced bronchoconstriction. Am. J. Respir. Crit. Care Med. 151: 2003-2005. Ansel, K. M., Djuretic, I., Tanasa, B. and Rao, A. (2006) Regulation of Th2 differentiation and Il4 locus accessibility. Annu. Rev. Immunol. 24: 607-656. Artis, D., Kane, C. M., Fiore, J., Zaph, C., Shapira, S., Joyce, K., MacDonald, A., Hunter, C., Scott, P. and Pearce, E. J. (2005) Dendritic cell-intrinsic expression of NF-
dc.identifier.uri	http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31345	-
dc.description.abstract	本研究首先為建立具有調節過敏免疫反應之食材的研究篩選模式，並評估其功效及可能的作用機轉。已知多數菇菌類食材對於免疫反應具有活化及調節之作用，故本實驗首先採用巴西洋菇、茯苓、樟芝與松杉靈芝等菇菌類為測試樣品，先以RBL-2H3肥大細胞株釋放組織胺能力、初代T細胞或TCR基因轉殖鼠分離出的T輔助細胞 (簡稱OVA特異性T細胞)分泌細胞激素能力，評估調節免疫功效。結果顯示，巴西洋菇、茯苓與松杉靈芝對組織胺的釋放無顯著影響，樟芝則促進組織胺釋放。此外，菇菌樣品均可促進T輔助細胞生成IL-2，且會抑制引發過敏疾病之Th2細胞激素的分泌。再以巴西洋菇、茯苓與松杉靈芝樣品餵食OVA致敏小鼠，結果顯示，三種菇菌樣品均可以降低呼吸道嗜伊紅白血球的聚集、IL-5的分泌與總蛋白質的濃度，對於脾臟細胞IL-4與IL-5的分泌，亦有抑制作用。因此，評估食材對過敏免疫反應之調節作用，以未分化的OVA特異性T輔助細胞，加入待測食材培養，分析分化過程中Th1與Th2細胞激素分泌量，為較準確且省時的篩選方式。接著以細胞培養的方式初步探討松杉靈芝有效減緩過敏反應之成分為何。先以RAW264.7巨噬細胞分泌TNFα、RBL-2H3 肥大細胞釋放組織胺、EL4 小鼠T 細胞株或OVA 特異性T 細胞分泌細胞激素、及含IL-4 promoter 質體轉染之T 細胞報導基因表現等，評估松杉靈芝多醣類與三萜類區分物對過敏免疫反應之調節。結果顯示，松杉靈芝多醣與三萜類可以顯著的活化RAW264.7 分泌TNFα。多醣類對於肥大細胞釋放組織胺無顯著影響，但三萜類可以顯著抑制組織胺的釋放。松杉靈芝在T 細胞分化過程中，多醣類對Th1 細胞的活化能力高於三萜類，三萜類對引發過敏疾病之Th2 免疫反應的抑制效果較佳。松杉靈芝多醣與三萜類均不影響IL-4 promoter 下游的報導基因表現 (luciferase activity)，但三萜類可以顯著的抑制EL4 細胞分泌IL-4 與IL-5。本實驗另採用含NF-κB 接合位的質體，探究NF-κB 轉錄活性與IL-4 生成之相關性，結果顯示，松杉靈芝三萜類可以顯著的抑制NF-κB 下游報導基因表現，若在PPARγ拮抗劑與三萜類區分物共同培養下， IL-4 分泌受抑制的情形，則有回復的現象。由此得知，對於過敏性免疫反應而言，三萜類是松杉靈芝中較有效抑制Th2 免疫反應之區分物。為探討松杉靈芝三萜類對體內T 細胞調節的影響，以及是否能夠減緩氣喘性過敏免疫反應。本實驗接著以OVA 進行腹腔注射與吸入性致敏，誘發過敏性氣喘小鼠研究模式進行動物實驗，並以固醇類藥物prednisolone 為對照組。結果顯示，松杉靈芝三萜類補充可以顯著減緩呼吸道過度反應 (AHR)，減少免疫細胞在肺沖洗液及呼吸道週邊組織的聚集、降低肺沖洗液中IL-5、eotaxin、IL-4 與IL-13 含量，以及減緩氣喘之局部性發炎的作用，與固醇類藥物類似。同時三萜類對血清特異性IgG1 抗體生成，與脾臟細胞分泌IL-4 及IL-5 也有抑制作用；但對血清IgG2a，及脾臟細胞生成Th1 細胞激素，則無顯著影響。由以上結果得知，松杉靈芝三萜類可以顯著減緩氣喘小鼠呼吸道過度反應與發炎反應，亦可以顯著的抑制Th2 免疫反應，但不影響Th1 免疫反應。綜合本研究之結果得知，由RBL-2H3 肥大細胞、OVA 特異性T 細胞、EL4 T 細胞株至動物實驗，松杉靈芝尤其是三萜類可有效的抑制組織胺釋放、呼吸道發炎介質生成、減緩氣喘小鼠呼吸道過度反應、抑制Th2 細胞激素分泌，與OVA 特異性IgG1 生成，但不影響Th1 免疫反應，顯示松杉靈芝三萜類可以主要抑制氣喘的過敏免疫反應。推論松杉靈芝三萜類可透過PPARγ活化，抑制NF-κB 之轉錄活性與Th2 免疫反應，具有開發為較具安全性的氣喘用藥之潛能。	zh_TW
dc.description.abstract	The purpose of this study was to establish both in vitro and in vivo systems to study and explore the possible functional foods for the allergic diseases. It has been well documented that fungi might exert immunomodulatory effect, and thus the Agaricus blazei, Poria cocos, Ganoderma tsugae and Antrodia camphorate were tested for allergic immunomodulatory effect, by using RBL-2H3 mast cell line, primary splenocyte form BALB/c; and OVA-specific CD4+ T cells from T cell receptor (TCR) transgenic DO11.10 mice (nanaïve OVA-specific T cells). The results showed that ionomycin-stimulated histamine production form RBL-2H3 cells did not affect by Agaricus blazei, Poria cocos and Ganoderma tsugae, but increased by Antrodia camphorate. OVA-stimulated IL-2 secretion form Th1 cells was increased, but IL-4 and IL-5 secretion from Th2 cells was decreased by all the fungi samples. Furthermore, fungi treatment increased the IL-2 level and decreased Th2 cytokines production during naïve OVA-specific T cell differentiation in vitro. To further investigate the effect of fungi on airway inflammation, the allergen-sensitized mice challenged with aerosol allergen supplemented with Agaricus blazei, Poria cocos or Ganoderma tsugae. The data showed that the percentage of eosinophils, IL-5 and total protein content in bronchoalvelor lavage fluid (BALF), and IL-4, IL-5 levels produced by splenocytes were significantly decreased in mice fed with Agaricus blazei, Poria cocos and Ganoderma tsugae. Furthermore, two major fractions of Ganoderma tsugae, triterpenoids (Gt-TRE) and polysaccharides (Gt-PS) were tested for their contribution for regulation of histamine and cytokines productions using several different cell models described above. The results showed that ionomycin-stimulated histamine secretion from RBL-2H3 cell was significantly suppressed by Gt-TRE, but not Gt-PS fraction. PMA/ionomycin-stimulated IL-4 secreted from a murine T cell line EL4 was also significantly decreased by Gt-TRE only. To further investigate whether Gt-PS and Gt-TRE affect CD4+ T cell polarization, naïve OVA-specific T cells were cultured with Gt-PS or Gt-TRE. The results showed that Gt-PS enhanced Th1 cytokine IL-2 but suppressed IFN-γ significantly. Gt-TRE had no effect on Th1 cytokines but dose-dependently suppressed Th2 cytokines more significantly than that of Gt-PS. To evaluate the effects of Ganoderma tsugae on IL-4 production, EL4 cell was transfected a plasmid containing reporter gene luciferase with IL-4 promoter. The results shown that luciferase activity did not affect by Gt-PS and Gt-TRE treatment, but IL-4 and IL-5 production were decreased by Gt-TRE. In addition, both EL4 cell and RAW264.7 macrophage cell line transfected a plasmid containing reporter gene luciferase with NF-κB binding sites promoter were cultured with Gt-PS or Gt-TRE. The results showed that the luciferase activity was significantly increased by Gt-PS but significantly decreased by Gt-TRE, suggesting that these two fractions may exert different effect on NF-κB related cytokine expression. An in vivo study was meant to investigate if triterpenoid extracts have anti-inflammatory effects on airway responses and regulatory effects on Th2 responses. BALB/c mice sensitized intra-peritoneally and challenged with OVA were treated with either TRE or prednisolone for 2 weeks. The effects of TRE on bronchial AHR, airway inflammation, serum antigen-specific antibody levels, and cytokine secretions from splenocytes were evaluated. TRE treatment significantly decreased AHR and reduced the total infiltrating leukocytes and eosinophils in BALF when compared to the control group. Furthermore, TRE decreased the production of inflammatory mediators, such as IL-4, IL-5, and eotaxin in BALF, as well as secretions from splenocytes of OVA-sensitized mice. TRE treatment also significantly decreased OVA-specific IgG1 production, but not IgG2a production. These data suggest that triterpenoids is the fraction of Ganoderma tsugae that alleviate bronchoalveolar inflammation in allergen-induced asthmatic animal model through the biological activity for suppression of Th2 responses. Overall, these data suggest that triterpenoid-rich extracts of Ganoderma tsugae attenuated histamine release, airway inflammation, airway hyperresponsiveness, Th2 cytokines secretion, and serum OVA-specific IgG1 production, but not affect Th1 responses. The regulatory mechanism may exert the NF-κB related Th2 cytokine expression through the PPARγ activation. Triterpenoid-rich extracts of Ganoderma tsugae exert anti-inflammatory effects on airway responses and attenuates Th2 responses without the overall immuno-suppression effects in allergic murine models of asthma.	en
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dc.description.tableofcontents	【目錄】目錄 I 圖目錄 VII 表目錄 IX 縮寫對照表 i 摘要 iii 英文摘要 (Abstract) v 第一章、文獻回顧第一節、過敏性疾病之簡介............................................................................ 1 一. 過敏性疾病之影響因子................................................................................ 1 二. 過敏性疾病之反應機制................................................................................ 2 三. T 細胞在過敏性疾病之角色.......................................................................... 4 四. 呼吸道過敏疾病之抗原致敏動物實驗模式................................................ 7 五. 過敏性疾病之治療........................................................................................ 9 六. 飲食因子影響過敏性疾病.......................................................................... 12 第二節、靈芝相關研究.................................................................................. 16 一. 靈芝之降血壓功能...................................................................................... 16 二. 靈芝之降血脂功能...................................................................................... 17 三. 靈芝預防肝損傷功用.................................................................................. 17 四. 靈芝促進傷口癒合...................................................................................... 17 五. 靈芝之抗氧化與抑制發炎反應.................................................................. 18 六. 靈芝之抗腫瘤活性...................................................................................... 19 七. 靈芝活化免疫細胞...................................................................................... 20 八. 靈芝抑制過敏免疫反應.............................................................................. 23 九. 靈芝減緩自體免疫疾病.............................................................................. 24 第三節、研究動機.......................................................................................... 27 第四節、研究架構與目的.............................................................................. 28 一. 研究架構...................................................................................................... 28 二. 研究目的...................................................................................................... 28 第二章、建立具有調節過敏免疫反應之細胞培養篩選模式第一節、前言................................................................................................... 31 第二節、材料與方法...................................................................................... 34 一. 材料.............................................................................................................. 34 二. 肥大細胞株培養.......................................................................................... 35 三. 組織胺含量分析.......................................................................................... 35 四. 脾臟細胞分離.............................................................................................. 36 五. BALB/c 脾臟細胞初代培養......................................................................... 36 六. OVA 特異性CD4+ T 細胞收集與純化........................................................ 36 七. OVA 特異性CD4+ T 細胞培養及樣品處理................................................ 37 八. 細胞激素含量分析...................................................................................... 38 九. 統計分析...................................................................................................... 39 第三節、結果................................................................................................... 39 一. 食材對肥大細胞釋放組織胺能力之影響.................................................. 39 二. 食材對T 細胞分泌細胞激素能力之影響.................................................. 41 第四節、討論................................................................................................... 48 第五節、結論................................................................................................... 50 第三章、菇菌樣品對OVA 致敏小鼠免疫反應之影響第一節、前言................................................................................................... 51 第二節、材料與方法...................................................................................... 51 一. 動物飼養...................................................................................................... 51 二. 致敏方式...................................................................................................... 52 三. 肺沖洗細胞樣品之收集.............................................................................. 52 四. 脾臟細胞收集.............................................................................................. 54 五. 腹腔細胞之收集與培養.............................................................................. 54 六. 細胞激素與發炎介質分析.......................................................................... 54 七. 非特異性抗體之分析.................................................................................. 55 八. 血清OVA 特異性抗體分析........................................................................ 56 (一). OVA 特異性IgE 分析.......................................................................... 56 (二). OVA 特異性IgG1 與IgG2a 分析........................................................... 56 九. TBARS 之測定............................................................................................. 57 十. 內生性及外因性PGE2 分析........................................................................ 58 (一). 內生性PGE2 之前處理........................................................................ 58 (二). 外因性PGE2 之前處理........................................................................ 58 (三). PGE2 含量分析...................................................................................... 58 十一. 統計分析.................................................................................................. 58 第三節、結果................................................................................................... 59 一. 生長及攝食效應.......................................................................................... 59 二. 呼吸道發炎介質.......................................................................................... 60 三. 血清抗體生成.............................................................................................. 60 四. 細胞激素分泌.............................................................................................. 61 五. 菇菌樣品降低脂質過氧化.......................................................................... 61 六. 內生性及外因性PGE2 生成........................................................................ 61 第四節、討論................................................................................................... 70 一. 菇菌類減緩呼吸道發炎反應...................................................................... 70 二. 細胞激素分泌方面...................................................................................... 70 三. 抗體生成方面.............................................................................................. 71 四. 抗氧化能力方面.......................................................................................... 72 五. 細胞培養篩模式與動物實驗模式之綜合討論.......................................... 72 第五節、結論................................................................................................... 74 第四章、以細胞培養模式評估松杉靈芝萃取物對過敏免疫反應之調節第一節、松杉靈芝活化巨噬細胞株RAW264.7 ........................................... 75 一. 前言............................................................................................................... 75 二. 材料與方法................................................................................................... 75 三. 結果............................................................................................................... 76 第二節、松杉靈芝三萜類抑制RBL-2H3 釋放組織胺................................ 78 一. 前言............................................................................................................... 78 二. 材料與方法................................................................................................... 78 三. 結果............................................................................................................... 79 第三節、EL4 小鼠T 細胞株活性分析.......................................................... 81 一. 前言............................................................................................................... 81 二. 材料與方法................................................................................................... 81 三. 結果............................................................................................................... 82 第四節、松杉靈芝萃取物調節OVA 特異性T 輔助細胞分化導向............ 84 一. 前言............................................................................................................... 84 二. 材料與方法................................................................................................... 84 三. 結果............................................................................................................... 85 第五節、以細胞短暫轉染方式評估三萜類對IL-4 生成之影響................. 91 一. 架構含有IL-4 promoter 質體之設計.......................................................... 91 二. 材料與方法................................................................................................... 91 三. 結果............................................................................................................... 96 1. pGL3-mIL4pro-Luc 質體架構................................................................. 96 2. pGL3-mIL4pro-Luc 質體短暫轉染EL4 T 細胞株.............................. 101 第六節、松杉靈芝三萜類對Th2 免疫反應之調控.................................... 105 一. 前言............................................................................................................. 105 二. 材料與方法................................................................................................. 107 三. 結果............................................................................................................. 109 1. 3 倍NF-κB site 轉染RAW264.7 細胞株............................................. 109 2. 3 倍NF-κB site 轉染EL4 小鼠T 細胞株........................................... 109 3. 松杉靈芝三萜類抑制NF-κB 轉錄活性之探討................................... 114 (A). 糖皮質固醇受器........................................................................... 114 (B). 過氧化體殖劑活化受器γ (PPARγ) ............................................... 114 第七節、討論................................................................................................. 118 一. 松杉靈芝區分物可活化免疫細胞............................................................. 118 二. 松杉靈芝三萜類降低肥大細胞釋放組織胺............................................. 118 三. 松杉靈芝萃取物對T 細胞之調節............................................................. 119 四. 松杉靈芝三萜類抑制NF-κB 之轉錄活性............................................... 120 五. 松杉靈芝三萜類與核受器......................................................................... 121 第八節、結論................................................................................................. 122 第五章、松杉靈芝三萜類對過敏疾病小鼠之影響第一節、前言................................................................................................. 123 一. 三萜類之生理功能..................................................................................... 123 二. 實驗動機..................................................................................................... 123 第二節、松杉靈芝三萜類對過敏體質小鼠免疫反應的影響.................... 126 一. 材料方法..................................................................................................... 126 (一). 實驗設計............................................................................................ 126 (二). 動物飼養............................................................................................ 126 (三). 致敏方法............................................................................................ 126 (四). 餵食樣品配製.................................................................................... 127 (五). 抗體分析............................................................................................ 127 (六). 脾臟細胞收集與培養........................................................................ 127 (七). 脾臟細胞增生.................................................................................... 127 (八). 脾臟細胞表面抗原分析.................................................................... 128 (九). 細胞激素分析.................................................................................... 128 (十). 統計分析............................................................................................ 128 二. 結果............................................................................................................. 129 第三節、松杉靈芝三萜類對氣喘小鼠過敏免疫反應之影響...................... 135 一. 材料方法..................................................................................................... 135 (一). 實驗設計............................................................................................ 135 (二). 動物飼養............................................................................................ 135 (三). 致敏方法............................................................................................ 136 (四). 餵食樣品配製.................................................................................... 136 (五). 氣喘小鼠呼吸道症狀測定................................................................ 136 (六). 肺沖洗細胞樣品之收集.................................................................... 137 (七). 肺組織切片........................................................................................ 137 (八). 脾臟細胞收集與培養........................................................................ 137 (九). 脾臟細胞增生.................................................................................... 137 (十). 脾臟細胞表面抗原分析.................................................................... 137 (十一). 血清抗體分析................................................................................ 137 (十二). 發炎介質與細胞激素含量分析.................................................... 137 (十三). 統計分析........................................................................................ 138 二. 結果............................................................................................................. 139 1. 松杉靈芝三萜類對組織相對重量之影響............................................. 139 2. 松杉靈芝三萜類對呼吸道發炎症狀之影響......................................... 140 3. 松杉靈芝三萜類對血清抗體生成之影響............................................. 147 4. 松杉靈芝三萜類對脾臟細胞表現與細胞激素分泌能力之影響......... 150 第四節、討論.................................................................................................. 157 一. 松杉靈芝三萜類降低氣喘之發炎病症..................................................... 157 二. 松杉靈芝三萜類對B 細胞表現及血清抗體生成的影響........................ 159 三. 松杉靈芝三萜類調節T 細胞之活性......................................................... 160 四. 松杉靈芝三萜類與固醇類藥物之比較..................................................... 162 第五節、結論.................................................................................................. 163 第六章、綜合討論與總結第一節、綜合討論.......................................................................................... 165 一. 松杉靈芝三萜類減緩氣喘病症................................................................. 165 二. 松杉靈芝多醣類活化Th1 免疫反應......................................................... 165 三. 松杉靈芝三萜類抑制過敏疾病Th2 傾向之免疫反應............................. 166 四. 松杉靈芝三萜類對Th2 細胞激素生成之調節......................................... 166 第二節、總結.................................................................................................. 168 附錄........................................................................................................................ 170 參考文獻................................................................................................................ 173
dc.language.iso	zh-TW
dc.title	松杉靈芝對過敏免疫反應之調節研究	zh_TW
dc.title	The Regulatory Effects of Ganoderma tsugae on Allergic Immune Responses	en
dc.type	Thesis
dc.date.schoolyear	95-1
dc.description.degree	博士
dc.contributor.oralexamcommittee	余俊強(Chun-Keung Yu),林金源(Jin-Yuarn Lin),吳文勉(Wen-M),江伯倫(Bor-Luan Chiang),許瑞祥(Ruey-Shyang Hseu),黃青真(Ching-Jang Huang)
dc.subject.keyword	氣喘,過敏免疫反應,松杉靈芝,三,	zh_TW
dc.subject.keyword	Asthma,Allergic immune responses,Ganoderma tsugae,Triterpenoids,	en
dc.relation.page	197
dc.rights.note	有償授權
dc.date.accepted	2006-10-30
dc.contributor.author-college	生命科學院	zh_TW
dc.contributor.author-dept	微生物與生化學研究所	zh_TW
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