請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31139完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 徐源泰(Yuan-Tay Shyu) | |
| dc.contributor.author | Hsiao-Yi Chen | en |
| dc.contributor.author | 陳效儀 | zh_TW |
| dc.date.accessioned | 2021-06-13T02:31:56Z | - |
| dc.date.available | 2009-02-02 | |
| dc.date.copyright | 2007-02-02 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-01-24 | |
| dc.identifier.citation | 王珍韶、李哖. 1992. 蝴蝶蘭的組織培養. 園藝作物組織培養實用技術.豐年社. p. 33-38.
朱宇敏. 2000. 臺灣產炭角菌類相概觀. Fungal Science 15:31-36. 李哖. 1990. 蘭之胚培養. 台灣園藝36:223-244. 李哖. 1992. 台灣原生蘭在園藝上的研究. 中央研究院植物研究所專 刊第十一號. p. 303-317. 李國基. 2001. 台灣金線連與蘭菌之鑑定及生產技術改進. 國立台灣 大學園藝學研究所博士論文. 吳聲華、周文能、王也珍. 2002. 台灣高等真菌；子囊菌與擔子菌的 認識. 國立台中自然科學博物館. 林良平. 1978. 土壤微生物學. 天然書社有限公司. 林維明. 1997. 野生蘭(1). 渡假出版社. 林維明. 2006a. 台灣野生蘭賞蘭大圖鑑(上). 天下遠見出版股份有限 公司. 林維明. 2006b. 台灣野生蘭賞蘭大圖鑑(下). 天下遠見出版股份有限 公司. 胡弘道. 1990. 林木菌根. 千華出版公司. 周玲勤. 2004. 台灣金線連、彩葉蘭和其F1雜交種之菌根生理與栽培. 國立台灣大學園藝學研究所博士論文. 曾千容. 2002. 石斛蘭蘭菌之分離、鑑定與培養及其對石斛蘭生長之 影響. 國立台灣大學園藝學研究所碩士論文. 張立宏. 1983. 台灣野生蘭(八)反捲根節蘭、黃根節蘭、黃花紅脣根節 蘭，白花根節蘭. 台灣花卉149:44-50. 黃宜瑩. 2005. 蘭科植物及其共生微生物之研究. 國立台灣大學園藝 學研究所碩士論文. 游璿樺. 2003. 多功能DNA分析系統(上). Bio-Rad. p.9-12. 蔡平里譯. 1991. 圖解蘭花繁殖最新技術. 淑馨出版社. 顏東敏. 1989. 蘭花繁殖技術. 東港鎮農會. 蘇鴻傑. 1990. 台灣野生根節蘭及鶴頂蘭之分類及生態研究 Ι . 根節 蘭屬美花根節蘭. 台大實驗林研究報告 4:143-160. 鐘詩文、許天銓、楊智凱. 2006. 台灣稀有蘭科植物圖鑑. 農委會林務 局. Arditti, J. 1967. Factors affecting the germination of orchid seeds. Bot. Rev. 33:1-97. Arditti, J. 1979. Aspects of the physiology of orchids. Adv. Bot. Res. 7: 421-665. Anderson, I. C. and J. W. G. Cairney. 2004. Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques. Environ. Microbiol. 6:769-779. Appels, R., W. L. Gerlach, E. S. Dennis, H. Swift, and W. J. Peacock. 1980. Molecular and chromosomal organization of DNA sequence coding for the ribosomal RNAs in cereals. Chromosoma 78:293-311. Bruns, T. 2006. Evolutionary biology: A kingdom revised. Nature 443: 758-761. Bougoure, D. S. and J. W. G. Cairney. 2005. Assemblages of ericoid mycorrhizal and other root-associated fungi from Epacris pulchella (Ericaceae) as determined by culturing and direct DNA extraction from roots. Environ. Microbiol. 7: 819-827. Bechtel, H., P. Cribb, and E. Lannert. 1986. The manual of cultivated orchid species. The MIT press, Cambridge, p. 72-74, 361-362. Bougoure, J. J., D. S. Bougoure, J. W. G. Cairney, and J. D. W. Dearnaley. 2005. ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (Orchidaceae) in southeastern Queensland. Mycol. Res. 109:452-460. Brundrett, M. C., A. Scade, A. L. Batty, K. W. Dixon, and K. Sivasithamparam. 2003. Devlopment of in situ and ex situ seed baiting techniques to detect myorrhizal from terrestrial orchid habitats. Mycol. Res. 107:1210-1220. Cairney, J. W. D. 2000. Evolution of mycorrhiza systems. Naturwissenschaften 87:467-475. Duncan, S., J. E. Barton, and P. A. O’Brien. 1993. Analysis of variation in isolates of Rhizoctonia solani by random amplification polymorphic DNA assay. Mycol. Res. 97:1075-1082. Fischer, S. G., and L. S. Lerman. 1983. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc. Natl. Acad. Sci. USA 80: 1579-1583. Fox, G. E., E. Stachebrandt, R. B. Hespell, J. Gibson, J. Maniloff, T. A. Dyer, R. S. Wolfe, W. E. Balch, R. S. Tanner, L. J. Magrum, L. B. Zablen, R. Blakemore, R. Gupta, L. Bonen, B. J. Lewis, D. A. Stahl, K. R. Luehrsen, K. N. Chen, and C. R. Woese. 1980. The phylogeny of prokaryotes. Science, New series, 209: 457-463. Hadley, G. 1970. Non-specificity of symbiotic infection in orchid mycorrhiza. New Phytol. 69:1015-1023. Hadley, G. 1982. Orchid mycorrhiza. In: Arditti (eds). Orchid biology: review and perspectives II. p. 83-118. Cornell University Press, Ithaca. New York. Hillis, D. M., and C. Moritz. 1990. An overview of applications of molecular systematics. p. 502-515. In: Molecular systematiics. D.M. Hillis and C. Moritz, eds. Sinauer Associates, Inc., Sunderland Massachusetts USA. Harley, J. L. and S. E. Smith. 1983. Mycorrhizal symbiosis. Academic Press, London. p. 334. Harman, G. E., C. R. Howell, A. Viterbo, I. Chet, and M. Lorito. 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nature Reveiws Microbiology 2:43-56. Julou, T., B. Burghardt, G. Gebauer, D. Berveiller, C. Damesin, and M.-A. Selosse. 2005. Mixotrophy in orchids: insights from a comparative study of green individuals and nonphotosynthetic individuals of Cephalanthera damasonium. New Phytol. 166: 639-653. James, T. Y., F. Kauff, C. L. Schoch, P. B. Matheny, V. Hofstetter, C. J. Cox, G. Celio, C. Gueidan, E. Fraker, J. Miadlikowska, H. T. Lumbsch, A. Rauhut, V. Reeb, A. E. Arnold, A. Amtoft, J. E. Stajich, K. Hosaka, G. H. Sung, D. Johnson, and B. Rourke. 2006. Reconstructing the early evolution of fungi using a six-gene phylogeny. Nature 443: 818-822. Knudson, L. 1922. A new nutrient solution for germination of orchid seed. Bot. Gaz. 73:1-25. Knudson, L. 1946. A new nutrient solution for germination of orchid seed. Amer. Orchid Soc. Bull. 15: 214-217. Kristiansen, K. A., J. V. Freudenstein, F. N. Rasmussen, and H. N. Rasmussen. 2004. Molecular identification of mycorrhizal fungi in Neuwiedia veratrifolia (Orchidaceae). Mol. Ecol. 33:251-258. Kristiansen, K. A., D. L. Taylor, R. Kjoller, H. N. Rasmussen, and S. Rosendahl. 2001. Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis (Orchidaceae) using SSCP and mitochondrial ribosomal LsDNA sequences. Mol. Ecol. 10: 2089-2093. Lewis, J. A. and R. D. Lumsden. 2001. Biocontrol of damping-off of greenhouse-grown crops caused by Rhizoctonia solani with a formulation of Trichoderma spp. Crop Protection 20: 49-56. Lilja, A., A. M. Hietala, and R. Karjalainen. 1996. Identification of a uninucleate Rhizoctonia sp. by pathogenicity, hyphal anastomosis and RAPD analysis. Plant Pathol. 45: 997-1006. Ma, M., T. K. Tan, and S. M. Wong. 2003. Identification and molecular phylogeny of Epulorhiza isolates from tropical orchids. Mycol. Res. 107: 1041-1049. McCormick, M. K., D. F. Whigham, and J. O'Neill. 2004. Mycorrhizal diversity in photosynthetic terrestrial orchids. New Phytol. 163:425-438. Myers, R. M., S. G. Fischer, L. S. Lerman, and T. Maniatis. 1985. Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucl. Acids Res. 13: 3131-3145. McCormick, M. K., D. F. Whigham, D. Sloan, K. O'Malley, and B. Hodkinson. 2006. Orchid-fungus fidelity: a marriage meant to last? Ecology 87: 903-911. Naseby, D. C., J. A. Pascual, and J. M. Lynch. 2000. Effect of biocontrol strains of Trichoderma on plant growth, Pythium ultimum populations, soil microbial communities and soil enzyme activities. J. of Appl. Microb. 88: 161-169. Ogoshi, A. 1996. In: Rhizoctonia species: taxonomy, molecular biology, ecology, pathology and disease control. Kluwer academic publishers. p. 1-5. Otero, J. T., D. J. Ackerman, and P. Bayman. 2002. Diversity and host specificity of endophytic Rhizoctonia-like fungi from tropical orchids. American Journal of Botany 89:1852-1858. Otero, J. T., D. J. Ackerman, and P. Bayman. 2004. Differentiation in mycorrhizal preferences between two tropical orchids. Mol. Ecol. 13: 2393-2404. Oniki, M., K. Kobayashi, T. Araki, and A. Ogoshi. 1986. A new disease of turf-grass caused by binucleate Rhizoctonia AG-Q. Ann. Phytophath. Soc. Japan 52:850-853. Pace, N. R., D. K. Smith, G. J. Olsen, and B. D. James. 1989. Phylogenetic comparative analysis and the secondary structure of ribonuclease RNA-a review. Gene 82:65-75. Read, D. J. 1991. Mycorrhizas in ecosystems. Experientia 47: 376-409. Rohlf, F. J., J. Kishpaugh, and D. Kirk. 1982. Numerical taxonomy system of multivariate statistical programs. State Univ. New York, Stony Brook, New York. Redecker, D., J. B. Morton, and T. D. Bruns. 2000. Ancestral lineages of arbuslular mycorrhizal fungi (Glomales). Mol. Phylogenet. Evol. 14:276- 284. Smith, S. E. and D. J. Read. 1997. Mycorrhizal symbiosis. 2nd edn. London: Academic Press. Sneh, B., L. Burpee, and A. Ogoshi. 1991. Identification of Rhizoctonia species. Amer. Phytopathol. Soc. Press, St. Paul, Minnesota p. 133. Selosse, M.-A., A. Faccio, G. Scappaticci, and P. Bonfante. 2004. Chlorophyllous and achlorophyllous specimens of Epipactis microphylla (Neottieae, Orchidaceae) are associated with ectomycorrhizal septomycetes, including truffles. Microb. Ecol. 47: 416-426. Selosse, M.-A., M. Wei, J.-L. Jany, and A. Tillier. 2002. Communities and populations of sebacinoid basidiomycetes associated with the achlorophyllous orchid Neottia nidus-avis (L.) L.C.M. Rich. and neighbouring tree ectomycorrhizae. Mol. Ecol. 11:1831-1844. Shefferson, R. P., M. Weiss, T. Kull, and D. L. Taylor. 2005. High specificity generally characterizes mycorrhizal association in rare lady's slipper orchids, genus Cypripedium. Mol. Ecol. 14: 613-626. Taylor, D. L., T. D. Bruns, and S. A. Hodges. 2004. Evidence for mycorrhizal races in a cheating orchid. Proceedings of the Royal Society: Biological Sciences 271: 35-43. Toda, T., T. Mushika, and M. Hyakumachi. 2004. Development of specific PCR primers for the detection of Rhizoctonia solani AG 2-2 LP from the leaf sheaths exhibiting large-patch symptom on zoysia grass. FEMS Microbiol. Lett. 232: 67-74. Taylor, D. L., T. D. Bruns, T. M. Szaro, and S. A. Hodges. 2003. Divergence in mycorrhizal specialization within Hexalectris spicata (Orchidaceae), a nonphotosynthetic desert orchid. American Journal of Botany 90:1168-1179. Taylor, T. N., W. Remy, H. Hass, and H. Kerp. 1995. Fossil arbuscular mycorrhizae from the early Devonian. Mycologia 87: 560-573. Wang, B. and Y.-L. Qiu. 2006. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 16:299-363. White, T. J., T. Bruns, S. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White, eds., PCR protocols : a guide to methods and applications, p. 315-322. Academic Press, New York, USA. Hopkins, G. W., P. A. Huner. Introduction to plant physiology. Yli-Mattila, T., S. Paavanen, A. Hannukkala, P. Parikka, R. Tahvonen, and R. Karjalainen. 1996. Isozyme and RAPD-PCR analysis of Fusarium avenaceum strains from Finland. Plant Pathol. 45:126-134. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31139 | - |
| dc.description.abstract | 蘭科植物的種子發育不完全，需依靠與真菌共生幫助種子發芽生長，所有的蘭科植物在生活史中均涉及與蘭菌的共生關係。本研究探討台灣地區原生蘭種根部共生真菌的菌種多樣性及各植株間菌相結構的差異，比較不同蘭種及地域對共生依存關係的影響。分離黃根節蘭及黃鶴頂蘭植株根部內生真菌共26株，以真菌核糖體RNA基因ITS區域序列作為菌種鑑定依據，並輔以UPGMA等系統演化方法以序列分析菌株間親源關係。結果顯示採集自阿里山黃鶴頂蘭與尖石鄉黃根節蘭植株根系菌相以炭角菌為主，其它植株根部菌相則呈現多樣性，較無特定的優勢菌種。菌相依宿主植株種類不同而有所差異，但以炭角菌屬廣泛存在於調查植株根部。另外，自其他18種台灣原生蘭種分離外表型態類似絲核菌的28株真菌菌株，經比對鑑定出被認定為蘭菌的絲核菌類真菌包括Rhizoctonia、Ceratobasidium及Thanatephorus等菌株，另分離出12株在蘭共生菌中研究較少的木黴菌。最後探討真菌核糖體RNA基因ITS 1/ITS 2區域以DGGE系統對菌株分型，可將相同菌種篩選出來，針對蘭科植物共生真菌提供
較快速的初步鑑定方法。 | zh_TW |
| dc.description.abstract | The minute seeds of orchids are nourished by endophytic fungi to germinate during the nonphotosynthetic early stages of development. All orchids studied so far have involved mycorrhizal in its life cycle. The unique characteristics and diversified species of orchid family may attribute to its distinctive relationship with mycorrhizal fungi. In this study, we have investigated the fungal diversity of Taiwan native orchids. Twenty-six isolated fungal endophytes associated with Calanthe sieboldii Decne. ex Regel and Phaius flavus (Blume) Lindl were analyzed for its ITS (internal transcribed spacer) composition for phylogenetic identification. A great variety of endophytic fungi were isolated from orchid roots. The majority of mycobionts were Xylaria and members of the Xylaria were the primary fungi in every roots sampled. Other 28 isolates were obtained from 18 Taiwan native orchid species and isolates were identified as Rhizoctonia, Ceratobasidium and Thanatephorus, which are generally regard as orchid mycorrhiza. The analysis of DGGE using the ITS 1/ITS 2 region showed that DGGE is useful for the rapid screening of orchid entophytes. This study has proposed a rapid way to identify and characterize of orchid associated fungi and potential to bring positive impact on orchid growth and development. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T02:31:56Z (GMT). No. of bitstreams: 1 ntu-96-R93628216-1.pdf: 868039 bytes, checksum: af08aaa0485700f1e0f8106326ecfafa (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 中文摘要…………………………………………………………i
英文摘要…………………………………………………………ii 目錄………………………………………………………………iii 圖目錄……………………………………………………………v 表目錄……………………………………………………………vii 壹、前言…………………………………………………………1 貳、前人研究……………………………………………………2 一、蘭科植物…………………………………………………2 二、研究對象…………………………………………………5 1. 黃根節蘭…………………………………………………5 2. 黃鶴頂蘭…………………………………………………6 三、菌根………………………………………………………6 四、蘭共生菌…………………………………………………9 五、蘭共生菌的分類…………………………………………12 六、蘭共生菌目前研究方向…………………………………14 七、分子鑑定－研究區域及技術……………………………15 1. 限制酶片段長度多型性(RFLP)…………………………20 2. 簡單重複序列(ISSR)……………………………………21 3. 單股構型多型性(SSCP)…………………………………21 八、變性梯度膠體電泳的原理及應用………………………22 參、材料與方法…………………………………………………25 一、研究材料…………………………………………………25 二、實驗藥品…………………………………………………25 三、儀器設備…………………………………………………26 四、實驗方法…………………………………………………27 五、實驗流程設計……………………………………………35 肆、結果與討論…………………………………………………38 ㄧ、真菌菌株ITS rDNA序列比對分析………………………38 二、黃根節蘭與黃鶴頂蘭共生菌相比較……………………44 三、絲核菌菌株篩選…………………………………………53 四、DGGE分型篩選……………………………………………62 1. 選擇分型區域……………………………………………62 2. 決定DGGE水平電泳條件…………………………………63 3. 利用DGGE對未知菌株分型與篩選………………………66 伍、結論…………………………………………………………75 陸、參考文獻……………………………………………………77 | |
| dc.language.iso | zh-TW | |
| dc.subject | 炭角菌 | zh_TW |
| dc.subject | 蘭共生菌 | zh_TW |
| dc.subject | 黃鶴頂蘭 | zh_TW |
| dc.subject | 黃根節蘭 | zh_TW |
| dc.subject | 菌相 | zh_TW |
| dc.subject | ITS | en |
| dc.subject | DGGE | en |
| dc.subject | orchid mycorrhiza | en |
| dc.title | 台灣原生蘭科植物菌根真菌多樣性之研究 | zh_TW |
| dc.title | Genetic Diversity of Root Associated Fungal Endophytes
From Taiwan Native Orchids | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張喜寧(Doris C.N. Chang),許輔(Fuu Sheu),曾文聖(Weng-Sheng Tseng) | |
| dc.subject.keyword | 蘭共生菌,黃鶴頂蘭,黃根節蘭,菌相,炭角菌, | zh_TW |
| dc.subject.keyword | orchid mycorrhiza,ITS,DGGE, | en |
| dc.relation.page | 84 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-01-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 847.69 kB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。