請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30762完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊西苑 | |
| dc.contributor.author | Wei-Shen Chen | en |
| dc.contributor.author | 陳威伸 | zh_TW |
| dc.date.accessioned | 2021-06-13T02:14:51Z | - |
| dc.date.available | 2007-04-26 | |
| dc.date.copyright | 2007-04-26 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-04-18 | |
| dc.identifier.citation | 參考資料:
1. Jill E. Hungerford,*Gary K. Owens,* W. Scott Argraves,and Charles D. Little†(1996) Development of the Aortic Vessel Wall as Defined by Vascular Smooth Muscle and Extracellular Matrix Markers..Developmental biology. 178, 375–392 2. Valerie Fatigati and RicharAd. Murphy(1984)Actin andTropomyosin Variants in Variantisn Smooth Muscles. DEPENDENCE ON TISSUE TYPE*. J. Biol. Chem. Vol. 259, No. 23, Issue of December 10, pp. 14383- 14388 3. Anna Ratajska, Monika Zarska , Christina Quensel, Jochen Krämer(2001) Differentiation of the smooth muscle cell phenotypes during embryonic development of coronary vessels in the rat. Histochem Cell Biol. 116:79–87 4. H. Scott Baldwin (1996) Early embryonic vascular development (review) . Cardiovascular Research 3 1 E34-E4.5 5. Jeanette M. Thayer, Kenneth Meyers, Cecilia M. Giachelli and Stephen M. Schwartz.(1995) Formation of the arterial media during vascular development. Cellular and Molecular Biology Research. Vol. 41, No.4, pp. 251-262 6. Olivier Kocher, Omar Skalli, Daniel Cerutti, Francoise Gabbiani, and Giulio Gabbiani. (1985) Cytoskeletal Features of Rat Aortic Cells during Development. An Electron Microscopic Immunohistochemical, and Biochemical Study. Circulation Research. Vol. 56, No. 6 7. Joseph M. Miano, Peter Cserjesi, Keith L. Ligon, Muthu Periasamy, Eric N. Olson(1994) Smooth Muscle Myosin Heavy Chain Exclusively Marks the Smooth Muscle Lineage During Mouse Embryogenesis. Circulation Research Vol 75, No 5 8. Jill E. Hungerforda Charles D. Littleb (1999) Developmental Biology of the Vascular Smooth Muscle Cell: Building a MultilayeredVessel Wall. J Vasc Res; 36:2–27 9. Jorg Wilting, Beate Brand-Saberi, Haymo Kurz, and Bodo Christ( 1995)Development of The Embryonic Vascular System. Cellular and Molecular Biology Research. Vol. 41, No.4, pp.219-232 10. Mats Hellström, Mattias Kalén1, Per Lindahl1, Alexandra Abramsson1 and Christer Betsholtz1(1999) Role of PDGF-B and PDGFR-b in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development 126, 3047-3055 11. H. Ulger, A. K. Karabulut and M. K. Pratten(2002)Labelling of Rat Endothethelial Cells with Antibodies to vWF, RECA-1, PECAM-1, ICAM-1, OX-43, and ZO-1. H. Anat. Histol. Embryol. 31, 31-51 12. Gordon R. Campbell, PhD; Julie H. Campbell, PhD; John A. Manderson, PhD; Sophie Horrigan; Robyn E. Rennick(1988) Arterial Smooth Muscle. A Multifunctional Mesenchymal Cell. Arch Pathol Lab Med, Vol. 112 13. Thomas J. Eddinger and Richard A. Murphy(1991) Developmental Changes in Actin and Myosin Heavy Chain Isoform Expression in Smooth Muscle. Archives of Biochemistry and Biophysics, Vol. 284, No. 2, February 1, pp. 232-237 14. Majesky, Mark W.; Schwartz, Stephen M.( 1997) An Origin for Smooth Muscle Cells From Endothelium?. Circulation Research, Vol. 80, 601-603, 15. Gary K. Owens(1995) Regulation of Differentiation of Vascular Smooth Muscle Cell. Physiological Reviews, Vol. 75, No. 3 16. Sawtell NM, Lessard JL(1989) Cellular distribution of smooth muscle actins during mammalian embryogenesis: Expression of the •-vascular but not the •-enteric isoform in differentiating striated myocytes. J Cell Biol, 109: 2929–2937 17. Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G(1986) A monoclonal antibody against •-smooth muscle actin: A new probe for smooth muscle differentiation. J Cell Biol, 103: 2787–2796 18. Westermark, B.; Siegbahn, A.; Heldin, C.; Claesson –Welsh , L. (1990) B-type receptor for platelet-derived growth factor mediatesa chemotactic response by means of ligand-induced activation of the receptor protein-tyrosine kinase. Proc. Natl. Acad. Sci. USA 87:128-132 19. M.C. de Ruiter, R.E. Poelmann, L. van Iperen, and A.C. Gittenberger-de Groot(1990) The early development of the tunica media in the vascular system of rat embryos. Anat. Embryol. 181: 341-349 20. Folkman J(1995) Angiogenesis in cancer, vascular, rheumatoid and other diseases. Nature Med, 1:27-31 21. Osborn M, Weber K(1982) Intermediate filaments:Cell-type specific markers in differentiation and pathology. Cell 31: 303-306 22. Olivetti G, Anversa P, Melissari M, Loud AV (1980) Morphometric study of early postnatal development of the thoracic aorta in the rat. Circ Res. 47: 417-424 23. Gerrity RG, Cliff WJ(1975) The aortic tunica media of the developing rat. I. Quantitative stereologic and biochemical analysis. Lab Invest 32: 585-600 24. Gabbiani G, Kocher O, Bloom WS, Vanderkerckhove J, Weber (1984) Actin expression in smooth muscle cells of rat aortic intimal thickening and human atheromatous plaque. J Invest 73: 148-152 25. Kocher O, Skalli O, Bloom WS, Gabbiani G(1984) The cytoskeleton of rat aortic smooth muscle cells. Normal conditions and experimental intimal thickening. Lab Invest SO: 645-652 26. Harald Herrmann* and Ueli Aebi(2000) Intermediate filaments and their associates: multi – talented structural elements specifying cytoarchitecture and cytodynamics. Current Opinion in Cell Biology 12:79–90 27. Ying-Hao Chou*,Satya Khuon*, Harald Herrmann†, and Robert D.Goldman*(2003) Nestin Promotes the Phosphorylation- dependent Disassembly of Vimentin Intermediate Filaments During Mitosis. Molecular Biology of the Cell , Vol. 14, 1468–1478 28. C.Wiese, A. Rolletschek, G. Kania, P. Blyszczuk, K. V. Tarasov, Y. Tarasov, R. P. Wersto, K. R. Boheler, and A. MWobus(2004) Nestin expression-a property of multi-lineage progenitor cells?. CMLS. 61, 2510-2522 29. Amy M. Kachinsky, Janice A. Dominov, and Jeffrey Boone Miller(1994) Myogenesis and the Intermediate Filament Protein, Nestin. Developmental Biology 165, 216-228 30. Goldman, R. D. and P. M. Steinert(1990) Cellular and Molecular Biology of Intermediate Filaments. Plenum Press, New York. 31. Silver J. and Miller J.H(2004) REGENERATION BEYOND THE GLIAL SCAR . Nat. Rev. Neurosci. 5: 146-156 32. Michalczyk K. and Ziman M(2005) Nestin structure and predicted function in cellular cytoskeletal organization. Histol. Histopathol. 20: 65-671 33. Dhe-Paganon, S., E.D. Werner, Y.I. Chi and S.E. Shoelson (2002) Structure of the globular tail of nuclear lamin. J. Biol. Chem. 277.20: 17381-17384 34. Gerace, L., A, Blum and G. Blobel(1978) Immunocytochemical localization of the major peptides of the nuclear pore complex-lamina fraction. Interface and mitotic distribution. J. Cell. Biol. 79 : 546-566 35. Jones, J.C.R., A.E. Goldman, H.Y. Yang and R.D. Goldman(1985) Distribution of intermediate filaments and their associated proteins during various stages of the mammalian cell cycle. Ann. N.Y. Acad. Sci. 455: 695-698 36. Steinert, P.M., Y.-H. Chou, V. Prahlad, D.A.D. Parry, L.N. MareKov, K.C.Wu, S.-I. Jang and R.D. Goldman(1999) A high molecular weight intermediate filament-associated protein in BHK-21 Cell is nestin, a type VI intermediate filament protein. J. Biol. Chem. 274. 9881-9890 37. Hockfield, S, R.D. Mckay(1985) Identification of major cell classes in the developing mammalian nervous system. J Neurosci 5:3310-3328 38. Kriho, V., H.Y. Yang, C.M. Lue, N. Lieska and G.D. Pappas(1996) An early developmental marker for radial glia in rat spinal cord. Proc. Microscopy and Microanalysis pp. 36-37 39. A. Margariti, L. Zeng and Q. Xu(2006) Stem cells, vascular smooth muscle cells and atherosclerosis. Histol. Histopathol. 21: 979-985 40. Sahlgren CM, Pallari HM, He T, Chou YH, Goldman RD, Eriksson JE (2006) A nestin scaffold links Cdk5/p35 signaling to oxidant- induced cell death. EMBO 25, 4808–4819 41. Hirschi K.K. and Majesky M.W. (2004). Smooth muscle stem cells. Anat. Rec. Discov. Mol. Cell. Evol. Biol. 276, 22-33 42. Frid M.G., Kale V.A. and Stenmark K.R (2002) Mature vascular endothelium can give rise to smooth muscle cells via endothelial- mesenchymal transdifferentiation: in vitro analysis. Circ. Res. 90, 1189-1196. 43. Ross R. and Glomset J.A. (1976a). The pathogenesis of atherosclerosis (first of two parts). N. Engl. J. Med. 295, 369-377. 44. Ross R. and Glomset J.A(1976b) The pathogenesis of atherosclerosis (second of two parts). N. Engl. J. Med. 295, 420-425. 45. Sahlgren C., Mikhailov A., Hellman J., Chou Y-H., Lendahl U., Goldman R. and Eriksson J (2001) Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase. J. Biol. Chem. 276, 16456-16463. 46. Liem R. K. (1993) Molecular biology of neuronal intermediate filaments. Curr. Opin. Cell Biol. 5: 12–16 47. Coffin, J.D.; Harrison, J.; Schwartz, S.; Heimark, R.(1991) Angioblast differentiation and morphogenesis of the vascular endothelium in the mouse embryo. Dev. Biol. 148:51-62 48. Risau, W.; Lemmon, V.(1988) Changes in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis. Dev. Biol. 125:441-450 49. Sang Hoon Lee, Jill E. Hungerford, Charles D. Little, and M. Luisa Iruela-arispe (1997) Proliferation and Differentiation of Smooth Muscle Cell Precursors Occurs Simultaneously During the Development of the Vessel Wall. Developmental Dynamics. 209:342-352 50. Owens G.K. and Thompson M.M. (1986) Developmental changes in isoactin expression in rat aortic smooth muscle cells in vivo. Relationship between growth and cytodifferentiation. J. Biol. Chem. 261, 13373-13380 51. VICTOR J. DZAU1, RUEDIGER C. BRAUN-DULLAEUS and DANIEL G. SEDDING (2002) Vascular proliferation and atherosclerosis : New perspectives and therapeutic strategies. NATURE MEDICINE, VOLUME 8 , NUMBER 11, 1249-1256 52. Hsi-Yuan Yang,*,† Norman Lieska,* Virginia Kriho,* Ching-Ming Wu,† and George D. Pappas*(1997)A Subpopulation of Reactive Astrocytes at the ImmediateSite of Cerebral Cortical Injury. Exp. Neurol.,146:199-205 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30762 | - |
| dc.description.abstract | 摘要
心血管系統在生理上所扮演的基本功能是藉由血液運送養分到身體各處的器官組織。一般來講，動脈管壁有三層構造，從血管內腔到最外層分別為intima、media、adventitia，而組織學上動脈依照media層的厚度和一些組織特徵又可分成三種，分別是彈性動脈、肌肉型動脈、小動脈血管。其中，彈性動脈的管壁和其它兩種血管相較而言有較多層elastic lamellae，而主動脈(aorta)和總頸動脈(common carotid artery)就是屬於彈性動脈。Nestin是中間絲蛋白，主要表現在胚胎時期的細胞，包括神經幹細胞、星形膠細胞的祖元細胞及未成熟的肌細胞。而且有報告指出中間絲蛋白nestin可用以標定不同時期的分化細胞，以及發育階段的細胞。 本實驗主要利用雙重免疫螢光染色與不同天數的胎鼠和剛出生的小鼠，來觀察發育期間大鼠總頸動脈的平滑肌細胞所含中間絲蛋白nestin表現的變化。結果發現在胚胎第13天(E13)時，總頸動脈裡面有一層由內皮細胞的前驅細胞所構成的內皮管，而且這些內皮細胞的前驅細胞會表現nestin。另外，內皮管外圍還散佈一些表現nestin的細胞，這些表現nestin的細胞可能是血管平滑肌細胞的前驅細胞(VSM progenitor)。在E14時，內皮管周圍的VSM progenitor會移行到內皮管周圍聚集，所以內皮管周圍就會聚集1~2層平滑肌細胞層。在E16時，聚集在內皮管周圍的平滑肌細胞開始表現SM-MHC。到E17時，內皮管周圍已聚集6~8層平滑肌細胞層。但是在發育第18天之後，血管平滑肌細胞中的nestin突然開始消失並且只表現SM-MHC。因此，nestin可作為發育中血管平滑肌細胞的分化標記。另外，針對nestin和SM-MHC的雙重免疫螢光染色觀察可發現，總頸動脈血管平滑肌細胞在發育過程中可分成三個階段：第一、發育初期，部份mesenchymal cells會轉變成表現nestin的VSM progenitors；第二、VSM progenitors分化形成同時表現nestin和SM-MHC的成熟中的血管平滑肌細胞(maturing VSMCs)；第三、maturing VSMCs成為只表現SM-MHC的已成熟血管平滑肌細胞(matured VSMCs)。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-13T02:14:51Z (GMT). No. of bitstreams: 1 ntu-96-R93b43018-1.pdf: 882544 bytes, checksum: a7cf906f6c786c453a669961fc83e4a1 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目次
頁數 致謝……………………………………………………………………1 摘要………………………………………………………………… 2-3 緒論 Ⅰ、引言 …………………………………………………………4-6 Ⅱ、動脈的構造與分類……………………………………………7 Ⅱ-1、血管(動脈和靜脈)的管壁構造………………………7-8 Ⅱ-2、動脈的分類……………………………………………8-9 Ⅲ、胚胎時期血管的發育…………………………………10-11 Ⅲ-1、胚胎時期血管的發育---內皮管的形成……………11-13 Ⅲ-2、胚胎時期血管的發育---血管平滑肌細胞的形成…13-14 Ⅳ、血管平滑肌細胞(VSMCs)……………………………15-17 Ⅴ、中間絲(Intermediate Filaments IFs)………17-20 Ⅵ、Nestin…………………………………………………20-22 實驗目的…………………………………………………………23 實驗材料與方法……………………………………………24-25 實驗結果………………………………………………………26-30 討論………………………………………………………………31-34 參考資料………………………………………………………35-41 附圖………………………………………………………………42-63 | |
| dc.language.iso | zh-TW | |
| dc.subject | 平滑肌細胞 | zh_TW |
| dc.subject | 總頸動脈 | zh_TW |
| dc.subject | nestin | en |
| dc.title | Nestin在大鼠總頸動脈平滑肌細胞
發育中的表現 | zh_TW |
| dc.title | Expression of Nestin in The Developing Vascular
Smooth Muscle Cells of Rat Common Carotid Artery | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃火煉,李心予 | |
| dc.subject.keyword | 總頸動脈,平滑肌細胞, | zh_TW |
| dc.subject.keyword | nestin, | en |
| dc.relation.page | 63 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-04-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 861.86 kB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。