請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30349
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李伯訓(Bor-Shiunn Lee) | |
dc.contributor.author | Yi-Ting Lai | en |
dc.contributor.author | 賴易廷 | zh_TW |
dc.date.accessioned | 2021-06-13T02:01:47Z | - |
dc.date.available | 2007-07-12 | |
dc.date.copyright | 2007-07-12 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-09 | |
dc.identifier.citation | Agrawal CM, Ray RB, Biodegradable polymeric scaffolds for musculoskeletal tissue engineering. Journal of Biomedical Materials Research 2001; 55(2):141-150.
Agrawal CM, Athanasiou KA, Technique to control pH in vicinity of biodegrading PLA–PGA implants. Journal of Biomedical Materials Research 1997; 38(2):105–114. Alliot-Licht B, De Lange GL, Gregoire M, Effect of Hydroxyapatite particles on periodontal ligament fibroblast-like cell behavior. Journal of Periodontology 1997; 68(2):158–165. Aubin JE, Bone stem cells. Review. Journal of Cellular Biochemistry Supplied 1998; 30-31:73-82. Athanasiou KA, Niederauer GG, Agrawal CM, Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid/polyglycolic acid copolymers. Biomaterials 1996; 17(2):93-102. Aubin JE, Heersche JM, Vitamin D and osteoblasts. In”Vitamin D” Academic Press, Dan Diego:313-328, 1997 Bab I, Gavish H, Namdar-Attar M, Muhlrad A, Greenberg Z, Chen Y, Mansur N, Shteyer A, Chorev M, Isolation of mitogenically active C-terminal truncated pentapeptide of osteogenic growth peptide from human plasma and culture medium of murine osteoblastic cells. Journal of Peptide Research 1999; 54(5):408-414. Baek SH, Kim S, Bone repair of experimentally induced through-and-through defects by Gore-Tex, Guidor, and Vicryl in ferrets:a pilot study. Oral Surgery Oral Medicine Oral Pathology Oral Radiology & Endodontics 2001; 91(6):710-714. Bergsma EJ, Rozema FR, Bos RM, De Bruijn WC, Foreign body reactions to resorbable poly(L-lactide) bone plates ad screws used for the fixation of unstable zygomatic fractures. Journal of Oral and Maxillofacial Surgery 1993; 51(6):666-670. Caffesse RG, Smith BA, Castelli WA, Nasjleti CE, New attachment achieved by guided tissue regeneration in beagle dogs. Journal of Periodontology 1988; 59(9):589-594. Chang TC, Wang JK, Hung MW, Chiao CH, Tsai LC, Chang GG, Regulation of the expression of alkaline phosphatase in a human breastcancer cell line. Journal of Biochemistry 1994; 303: 100-205 Cooke FW, Ceramics in orthopedic surgery. Clinical Orthopaedics & Related Research 1992; 276:135–146 Coombes AG, Rizzi SC, Williamson M, Barralet JE, Downes S, Wallace WA, Precipitation casting of polycaprolactone for applications in tissue engineering and drug delivery. Biomaterials 2004; 25(2):315-325 Craighead HG, Turner SW, Davis RC, James CD, Perez AM, John PM, Isaacson MS, Kam L, Shain W, Turner JN, Banker G, Chemical and topographical surface modification for control of central nervous system cell adhesion. Biomedical microdevices 1998; 1: 49–64. Cuneyt TA, Korkusuz F, Timicin M, Akkas N, An investigation of the chemical synthesis and high-temperature sintering behaviour of calcium hydroxyapatite (HA) and tricalcium phosphate (TCP) bioceramics. Journal of Materials Science: Materials in Medicine 1997; 8(2):91-96. Dahlin C, Gottlow J, Lindhe A, Nyman S. Healing of bone defects by guided tissue regeneration. Plastic & Reconstructive Surgery 1988; 81(5):672-676. Damien CJ, Parsons JR, Bone graft and bone graft substitutes. A review of current technology and applications. Journal of Applied Biomaterials 1991; 2(3):187–208. Egelberg J, Regeneration and repair of periodontal tissues. Journal of Periodontal Research 1987; 22(3):233-242. Fazzi R, Galimberti S, Testi R, Pacini S, Trasciatti S, Rosini S, Petrini M, Bone and bone marrow interactions: hematological activity of osteoblastic growth peptide (OGP)-derived carboxy-terminal pentapeptide(II). Action on human hematopoietic stem cells. Leukemia Research 2002; 26(9):839-848. Franceschi RT, James MW, Zerlauth G, 1α,25-dihydroxyvitamin D3 specific regulation of growth, morphology, and fibronectin in a human osteosarcoma cell line. Journal of Cellular Physiology 1985; 123(3):401-409. Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AG, Langer R, Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymer. Journal of Biomedical Materials Research 1993; 27(1):11-23. Fujihara K, Kotaki M, Ramakrishna S, Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers. Biomaterials 2005; 26(19):4139-4147. Gabriel M, Amerongen GP, Van Hinsbergh VW, Amerongen AV, Zentner A, Direct grafting of RGD-motif-containing peptide on the surface of polycaprolactone films. Journal of Biomaterials Science, Polymer Edition. 2006; 17(5):567-577. Gorbunoff MJ, The interaction of proteins with hydroxyapatite I:Role of protein charge and structure. Analytical Biochemistry 1984; 136(2):425-432. Gorbunoff MJ, The interaction of proteins with hydroxyapatite II:Role of protein acid and basic group. Analytical Biochemistry 1984; 136(2):433-439. Gorbunoff MJ, The interaction of proteins with hydroxyapatite III:Mehanism. Analytical Biochemistry 1984; 136(2):440-445. Gottlow J, Nyman S, Lindhe J, Karring T, Wennstrijm J. New attachment formation in the human periodontium by guided tissue regeneration. Case reports. Journal of Clinical Periodontology 1986; 13(6):604-616. Greenberg Z, Gavish H, Muhlrad A, Isolation of osteogenic growth peptide from osteoblastic MC3T3 E1 cell cultures and demonstration of osteogenic growth peptide binding proteins. Journal of Cellular Biochemistry 1997; 65(3):359-367. Gurevitch O, Slavin S, Muhlrad A, Shteyer A, Gazit D, Chorev M, Vidson M, Namdar-Attar M, Berger E, Bleiberg I, Bab I, Osteogenic growth peptide increases blood and bone marrow cellularity and enhances engraftment of bone marrow transplants in mice. Blood 1996; 88(12):4719-4724. Glickman I, Fermin AC, Michael GN, Philadelphia, WB, Reconstructive osseous surgery. In textbook of Clinical periodontology. 8th., 1996 pp. 622-636. Ignatius AA, Ohnmacht M, Claes LE, Kreidler J, Palm F. A composite polymer/tricalcium phosphate membrane for guided bone regeneration in maxillofacial surgery. Journal of Biomedical Materials Research 2001; 58(5):564-569. Imbronito AV, Todescan JH, Carvalho CV, Arna-Chavez VE, Healing of alveolar bone in resorbable and non-resorbable membrane-protected defects. A histologic pilot study in dogs. Biomaterials 2002; 23(20):4079–4086. Ishaug SL, Crane GM, Miller MJ, Yasko AW, Yaszemski MJ, Mikos AG, Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. Journal of Biomedical Materials Research 1997; 36(1):17-28. Ito M, Hidaka Y, Nakajima M, Yagasaki H, Kafrawy AH, Effect of hydroxyapatite content on physical properties and connective tissue reactions to a chitosan-hydroxyapatite composite membrane. Journal of Biomedical Materials Research 1999; 45(3):204-208. Jansen JA, Ruijter JE, Janssen PT, Paquay YG, Histological evaluation of a biodegradable Polyactive/hydroxyapatite membrane. Biomaterials 1995; 16(11):819-827. Kikuchi M, Koyama Y, Yamada T, Imamura Y, Okada T, Shirahama N, Akita K, Takakuda K, Tanaka J, Development of guided bone regeneration membrane composed of beta-tricalcium phosphate and poly (L-lactide-co-glycolide-co-epsilon-caprolactone) composites. Biomaterials 2004; 25(28):5979-5986 Kim HW, Leea EJ, Kima HE, Salihb V, Knowlesb JC, Effect of fluoridation of hydroxyapatite in hydroxyapatite-polycaprolactone composites on osteoblast activity. Biomaterials 2005; 26(21):4395–4404 Kilpadi DV, Lemons JE, Surface energy characterization of unalloyed titanium implants. Journal of Biomedical Materials Research 1994; 28(12):1419-1425. Kumar R, Prakash KH, Cheang P, Khor KA, Temperature driven morphological changes of chemically precipitated hydroxyapatite nanoparticles. Langmuir 2004; 20(13):5196-5200. Jan LP, Ito A, Tateishi T, Sol-gel synthesis of amorphous calcium phosphate and sintering into microporous hydroxyapatite bioceramics. Journal of American Ceramic Socity 1998; 81:1421-1428. Lee BS, Lin CP, Hung YL, Lan WH, Structural changes of Er:YAG laser-irradiated human dentin. Photomedicine and Laser Surgery 2004; 22(4):330-334. LeGeros, Zapanta R, Calcium phosphates in oral biology and medicine p78~82 Basel ; New York : Karger, c1991 Lindhe J, Reattachment-New attachment. In: Textbook of clinical periodontology. 2nd ed., Loe H, Gothenburg, Munksgaard, Copenhagen. pp. 462-473, 1989. Liu HS, Chin TS, Lai LS, Chiu SY, Chumg KH, Chang CS, Lui MT, Hydroxyapatite synthesized by a simplified hydrothermal method. Ceramic International 1997; 23:19-25. Locci P, Calvitti M, Belcastro S, Phenotype expression on gingival fibroblast cultured on membrane used in guided tissue regeneration. Journal of Periodontalogy 1997; 68:857-863. Mann BK, Schmedlen RH, West JL, Tethered-TGF-beta increases extracellular matrix production of vascular smooth muscle cells. Biomaterials 2001; 22(5):439-444. Mann BK, Tsai AT, Scott-Burden T, West JL, Modification of surfaces with cell adhesion peptides alters extracellular matrix deposition. Biomaterials 1999; 20(23-24): 2281-2286. Marletta G, Ciapetti G, Satriano C, Pagani S, Baldini N, The effect of irradiation modification and RGD sequence adsorption on the response of human osteoblasts to polycaprolactone. Biomaterials 2005; 26(23):4793-4804. Marra KG, Szem JW, Kumta PN, DiMilla PA, Weiss LE, In vitro analysis of biodegradable polymer blend/hydroxyapatite composites for bone tissue engineering. Journal of Biomedical Materials Research 1999; 47:324–335. Massia SP, Hubbell JA, An RGD spacing of 440nm is sufficient for integrin av, X3-mediated fibroblast spreading and 140nm for focal contact and stress fiber formation. Journal of Cell Biology 1991; 114(5): 1089-1100. Mendieta C, Williams R, Periodontal regeneration with bioresorbable membranes. Current Opinion in Periodontology, 1994, 157-167 Mooney DJ, Baldwin DF, Suh NP, Vacanti JP, Langer R, Novel approach to fabricate porous sponges of poly(D,L-lactic-co-glycolic acid) without the use of organic solvents. Biomaterials 1996; 17(14):1417-1422. Myron N, James TM, Surgical complications in guided tissue regeneration. In: Periodontal therapy. Solaro B, Chicago, Quintessence Publishing Co, Inc., volume 1, pp. 265-277, 1998. Nuttall ME, Gimble JM, Is there a therapeutic opportunity to either prevent or treat osteopenic disorders by inbibiting marrow adipogenesis? Bone 2000; 27(2):177-184. Nygren H, Kinetics of antibody binding to surface-immobilized antigen. Analysis of data and an empiric model. Biophysical Chemistry 1994; 52(1): 45-50. Park GE, Pattison MA, Park K, Webster TJ, Accelerated chondrocyte functions on NaOH-treated PLGA scaffolds. Biomaterials 2005; 26(16):3075-3082. Payne JM, Cobb CM, Rapley JW, Migration of human gingival fibroblasts over guided tissue regeneration barrier materials. Journal of Periodontalogy 1996; 67(3):236-244. Piattelli A, Scarano A, Russo P, Matarasso S, Evaluation of guided bone regeneration in rabbit tibia using bioresorbable and non-bioresorbable membranes. Biomaterials 1996; 17(8):791–796. Piattelli A, Franco M, Ferronato G, Santello MT, Martinetti R, Scarano A, Resorption of composite polymer - hydroxyapatite membranes: A time-course study in rabbit. Biomaterials 1997; 18(8):629–633. Polson AM, Periodontal regeneration. Current status and directions. Quintessence, Chicago, 1994. Ramachandra RR, Roopa HN, Kannan TS. Solid state synthesis and thermal stability of HAP and HAP-β-TCP composite ceramic powders. Journal of Biomedical Materials Research 1997; 8: 511-518. Rhee SH, Tanaka J, Hydroxyapatite coating on a collagen membrane by a biomimetic method. Journal American Ceramic Socity 1998; 81: 3029-3031. Rich A, Harris AK, Anomalous preferences of cultured macrophages for hydrophobic and roughened substrata. Journal of Cell Science. 1981; 50: 1-7. Rulmont A, Belkebir A, Rocha J, Esculcas AP, Berthet P, Poisson S, Gilbert B, Gabelica Z, Llabres G, Wijzen F, Structural characterization of glassy phases in the system Na2O-Ga2O3-P2O5 by MAS-NMR, EXAFS and vibrational spectroscopy I. Cations coordination. Spectrochimica Acta. Part A, Molecular & Biomolecular Spectroscopy 2000; 56(3): 423-434. Saulnier B, Ponsart S, Coudane J, Garreau H, Vert M, Lactic acid-based functionalized polymers via copolymerization and chemical modification. Macromolecular Bioscience 2004; 4(3): 232-237. Schiller C, Epple M, Carbonated calcium phosphates are suitable pH-stabilising fillers for biodegradable polyesters. Biomaterials 2003; 24(12): 2037–2043. Schmitz JP, Hollinger JO, Milam SB, Reconstruction of bone using calcium phosphate bone cements: a critical review. Journal of Oral & Maxillofacial Surgery 1999; 57(9): 1122-1126. Shakibaei M, De Souza P, Merker HJ, Integrin expression and collagen type II implicated in maintenance of chondrocyte shape in monolayer culture: an immunomorphological study. Cell Biology International 1997; 21(2): 115-125. Shikinami Y, Okuno M, Bioresorbable devices made of forged composites of hydroxyapatite particles and poly l-lactide (PLLA): part I basic characteristics. Biomaterials 1999; 20(9): 859–877. Silverman RP, Bonasser L, Passaretti D, Randolph MR, Yaremchuk MJ, Adhesion of Tissue-Engineered Cartilage to Native Cartilage. Plastic Reconstruct Surgery 2000; 105(4): 1393-1397. Socrates G, Infrared Characteristic Group Frequencies Tables and Charts, Second Edition 1998; 178~180. Strathmann H, Membrane separation processes. Review article. Journal of membrane science 1981; 9: 121~189. Termine JD, Non-collagen proteins in bone. Ciba Foundation Symposium 1988; 136: 178-202. Tsuji H, Ikada Y, Blends of aliphaticpolyesters. II. Hydrolysis of solution-cast blends from poly (L-lactide) and poly(ε-caprolactone) in phosphate-buffered solution. Journal of Applied Polymer Science 1998; 67(3): 405-415. Ural E, Kesenci K, Fambri L, Migliaresi C, Piskin E, Poly(D,L lactide/- caprolactone)/hydroxyapatite composites. Biomaterials 2000; 21(21): 2147-2154. Verheyen CC, Wijn JR, Blitterswijk CA, Groot K, Evaluation of hydroxylapatite/poly(L-lactide) composites: Mechanical behavior. Journal of Biomedical Materials Research 1992; 26: 1277-1296. Wang HL, Miyauchi M, Takata T, Initial attachment of osteoblasts to various guided bone regeneration membranes: an in vitro study. Journal of Periodontal Research 2002; 37(5): 340-344. Weiner S, Traub W, Crystal size and organization in bone. Connect Tissue Research 1989; 21(1-4): 259–265. Weiss MV, Cole DEC, Ray K, Whyte MP, Lafferty MA, Mulivor RA, Harris H, A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proceedings of the National Academy of Sciences 1988; 85:7666-7669. Whyte MP, Vrabel LA, Infantile hypophosphatasia fibroblasts proliferate normally in culture: evidence against a role for alkaline phosphatase (tissue nonspecific isoenzyme) in the regulation of cell growth and differentiation. Calcified Tissue International 1987; 40(1): 1-7. Yoon K, Golub E, Rodan GA, Alkaline phosphatase cDNA transfected cells promote calcium and phosphorous deposition. Connect Tissue Research 1989; 22: 17-25. Zhang R, Ma PX, Porous poly(L-lactide)/apatite composites created by biomimetic process. Journal of Biomedical Materials Research 1999; 45(4): 285–293. Zhao S, Pinholt EM, Madsen JE, Donath K, Histological evaluation of different biodegradable and non-biodegradable membranes implanted subcutaneously in rats. Journal of Cranio-Maxillo-Facial Surgery 2000; 28(2): 116-122. Zhu Y, Gao C, Liu X, Shen J, Surface modification of polycaprolactone membrane via aminolysis and biomacromolecule immobilization for promoting cytocompatibility of human endothelial cells. Biomacromolecules 2002; 3(6): 1312-1319. Zhao G, Raines AL, Wieland M, Schwartz Z, Boyan BD. Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography. Biomaterials. 2007; 28(18): 2821-9. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30349 | - |
dc.description.abstract | 組織再生膜(GTR)可以阻隔生長較快之上皮組織侵入骨質缺損處而影響癒合,目前應用主要之材料以不可吸收性之組織再生膜為主(例如:ePTFE, Gore-Tex®),但是其生物不可吸收性,需要二次手術將其移除,因此許多人開始研究生物可吸收性之膠原蛋白膜或是高分子聚合膜之材料,膠原蛋白膜雖然有良好之生物相容性,但是其機械強度不足,分解速度與薄膜性質較無法控制,而高分子聚合膜雖然可以改善機械性質,但是生物相容性不佳。本研究的目的主要是自行設計組織再生膜,由具有高度生物相容性的生物陶瓷-氫氧基磷灰石,並在其表面接上骨質生長因子- sOGP10~14,以MG63細胞培養於複合薄膜之上觀察其貼附與存活率之情況,以及檢測鹼性磷酸酶酵素之活性,再以即時定量反轉錄聚合酶鏈式反應(Real-time Quantitative PCR)檢測成骨基因之表現。本研究所自行合成之氫氧基磷灰石結晶已由X光粉末繞射儀檢測確定,在複合薄膜的製備上則由傅利葉紅外線光譜儀(FTIR)檢測證實薄膜表面有聚己內脂多元醇與氫氧基磷灰石之表現,於MG63之培養上,發現複合薄膜在細胞的貼附與存活率測試上比未改質過之薄膜較好,於Real Time-PCR與鹼性磷酸酶活性檢測之實驗中可發現複合薄膜上之氫氧基磷灰石對於鹼性磷酸酶與骨鈣素也有促進之現象。由以上之結果可以得到以氫氧基磷灰石與sOGP10~14進行薄膜表面之改質證實對於細胞之貼附與成骨基因之表現有顯著改善的情況,以及混有氫氧基磷灰石之複合薄膜有應用於牙周骨質再生之潛力。 | zh_TW |
dc.description.abstract | Guided tissue regeneration (GTR) is a technique which is used for the treatment of bone defects associated with periodontal disease or dental implants. Currently, non-resorbable materials (ex:ePTFE, Gore-Tex®) are used for the fabrication of the membranes. However, a disadvantage of non-resorbable membranes is that they require a second surgical session for the removal of the membrane. Conversely, biodegradable membranes such as collagen and synthetic biodegradable polymers have been studied by many researchers. Collagen membrane has excellent cell affinity and biocompatibility to regenerate tissues; however, its mechanical strength is poor. Biodegradable polymers exhibit improved mechanical properties but cell affinity is not good. Therefore, the aim of this study was to fabricate a composite which is composed of a bioresorbable polymer (Polycaprolactone) and bioactive ceramic (Hydroxyapatite). In addition, osteogenic growth peptide (sOGP10~14) was immobilized on the surface of composite. The osteoblasts activity of MG63 cells including cell attachment, viability, and calcification related gene and enzyme was examined. The characteristic peaks of X-ray diffraction were identified as hydroxyapatite. Successful combination of polycaprolactone and hydroxyapatite was demonstrated by FTIR analysis. Cell adhesion and cell viability onto PCL+HAP+sOGP10~14 composite membrane was elevated after surface modification. The alkaline phosphates enzyme activity and the gene expression level osteocalcin were up regulated by hydroxyapatite. In summary, our results suggest that HAP and sOGP10~14 plays a significant role in stimulating the bone derived cellular activity and hydroxyapatite composite membrane have high potential to be used for hard tissue regeneration. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T02:01:47Z (GMT). No. of bitstreams: 1 ntu-96-R93422001-1.pdf: 3396498 bytes, checksum: 36868d0cd0bb866c4b06e67d5c2aba72 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 中文摘要---------------------------------------------------------------------------------------------I
Abstract----------------------------------------------------------------------------------------------II 目錄-------------------------------------------------------------------------------------------------IV 圖次-------------------------------------------------------------------------------------------------IX 第一章 前言----------------------------------------------------------------------------------------1 第二章 文獻回顧----------------------------------------------------------------------------------2 2.1 引導組織再生術---------------------------------------------------------------------------2 2.2 組織再生膜之發展------------------------------------------------------------------------3 2.2.1 生物不可吸收之組織再生膜------------------------------------------------------ 3 2.2.2 生物可吸收之組織再生膜----------------------------------------------------------3 2.2.2.1 膠原蛋白--------------------------------------------------------------------------3 2.2.2.2 非膠原蛋白-----------------------------------------------------------------------4 2.3 組織再生膜的發展目標------------------------------------------------------------------5 2.4 複合材料之組織再生膜------------------------------------------------------------------5 2.4.1 聚己內酯多元醇(Polycaprolactone;PCL)---------------------------------------6 2.4.2 氫氧基磷灰石(Hydroxyapatite)---------------------------------------------------7 2.5 高分子聚合薄膜之製備方法------------------------------------------------------------8 2.6 組織再生膜之表面改質------------------------------------------------------------------9 2.7 生長因子(Growth Factor)--------------------------------------------------------------10 2.7.1 胜肽類(Peptide)--------------------------------------------------------------------11 2.7.1.1 RGD-------------------------------------------------------------------------------11 2.7.1.2 Synthetic OGP-derived pentapeptide (sOGP10-14: Tyr-Gly-Phe-Gly-Gly)--------------------------------------11 2.7.2 蛋白質(Protein)---------------------------------------------------------------------12 第三章 動機與目的-----------------------------------------------------------------------------13 第四章 材料與方法-----------------------------------------------------------------------------14 4.1 氫氧基磷灰石之合成與性質分析-----------------------------------------------------14 4.1.1 氫氧基磷灰石製備(Synthesis of Hydroxyapatite)----------------------------14 4.1.2 氫氧基磷灰石(HAP)之性質分析-----------------------------------------------15 4.1.2.1 掃描式電子顯微鏡之觀察(SEM)------------------------------------------15 4.1.2.2 穿透式電子顯微鏡之觀察(TEM)------------------------------------------15 4.1.2.3 X光繞射分析(XRD)----------------------------------------------------------15 4.1.2.4 粒徑大小分析------------------------------------------------------------------16 4.2 聚己內酯多元醇-氫氧基磷灰石複合薄膜之製備與性質分析-------------------16 4.2.1 複合薄膜之製備--------------------------------------------------------------------16 4.2.1.1 聚己內酯多元醇薄膜之製備------------------------------------------------16 4.2.1.2 聚已內酯多元醇-氫氧基磷灰石複合薄膜之製備-----------------------17 4.2.2 複合薄膜之性質分析--------------------------------------------------------------17 4.2.2.1 掃描式電子顯微鏡之觀察(SEM)------------------------------------------17 4.2.2.2 傅立葉轉換紅外光譜儀(FTIR)檢測表面官能基-----------------------17 4.3 複合薄膜之表面改質--------------------------------------------------------------------17 4.3.1 複合薄膜之表面改質方法--------------------------------------------------------18 4.3.1.1 胺解(Aminolysis)-------------------------------------------------------------18 4.3.1.2 胜肽接枝(Peptide grafting)--------------------------------------------------18 4.3.2 表面改質後複合薄膜表面性質分析--------------------------------------------19 4.3.2.1 紅外線光譜儀分析(FTIR)---------------------------------------------------19 4.3.2.2 接觸角量測儀(Surface Contact Analyzer)--------------------------------19 4.4 改質後複合薄膜之生物測試(in vitro study)----------------------------------------20 4.4.1 MG63細胞培養(MG63 cell culture)---------------------------------------------20 4.4.2 材料消毒方法-----------------------------------------------------------------------20 4.4.3 細胞貼附行為觀察(Cell adhesive behavior)-----------------------------------20 4.4.3.1 樣本準備方法------------------------------------------------------------------20 4.4.3.2 細胞與材料貼附測試---------------------------------------------------------21 4.4.3.3 細胞貼附行為之觀察---------------------------------------------------------21 4.4.4 細胞存活率測試--------------------------------------------------------------------21 4.4.4.1 樣本準備方法------------------------------------------------------------------22 4.4.4.2 MTT assay-----------------------------------------------------------------------22 4.4.4.3 統計分析------------------------------------------------------------------------22 4.4.5 鹼性磷酸酶活性測試--------------------------------------------------------------23 4.4.5.1 樣本之準備---------------------------------------------------------------------23 4.4.5.2 抽取cell lysate------------------------------------------------------------------23 4.4.5.3 定量DNA------------------------------------------------------------------------24 4.4.5.4 鹼性磷酸酶活性測量---------------------------------------------------------24 4.4.5.5 統計分析------------------------------------------------------------------------25 4.4.6 Real time PCR檢測------------------------------------------------------------------25 4.4.6.1 樣本之準備---------------------------------------------------------------------25 4.4.6.2 Total RNA之萃取--------------------------------------------------------------26 4.4.6.3 RNA定量-------------------------------------------------------------------------26 4.4.6.4 RNA反轉錄成cDNA(Reverse Transcription)-----------------------------27 4.4.6.5 Real time Quantitative PCR---------------------------------------------------27 4.4.6.6 統計分析------------------------------------------------------------------------28 第五章 實驗結果--------------------------------------------------------------------------------29 5.1 氫氧基磷灰石粉末性質分析-----------------------------------------------------------29 5.1.1 HAP粉末之掃描式電子顯微鏡觀察---------------------------------------------29 5.1.2 HAP粉末之X光繞射分析試驗----------------------------------------------------29 5.1.3 HAP粉末之穿透式電子顯微鏡之觀察------------------------------------------30 5.1.4 HAP粉末之粒徑分析儀測量------------------------------------------------------30 5.2 聚己內脂多元醇薄膜性質分析--------------------------------------------------------30 5.2.1 PCL膜薄之電子顯微鏡觀察-------------------------------------------------------30 5.2.2 PCL薄膜傅立葉轉換紅外光譜儀分析-------------------------------------------31 5.3 聚己內酯多元醇-氫氧基磷灰石複合薄膜性質分析-------------------------------31 5.3.1 PCL+HAP複合薄膜之電子顯微鏡觀察-----------------------------------------31 5.3.2 PCL+HAP複合薄膜傅立葉轉換紅外光譜儀分析-----------------------------31 5.4 PCL+HAP表面改質之性質分析-------------------------------------------------------32 5.5 接觸角量測儀之分析--------------------------------------------------------------------32 5.6 MG63細胞對薄膜材料之貼附行為觀察----------------------------------------------33 5.6.1 12小時之觀察比較------------------------------------------------------------------33 5.6.2 24小時之觀察比較------------------------------------------------------------------34 5.7 細胞存活率分析--------------------------------------------------------------------------34 5.8 鹼性磷酸酶活性測量--------------------------------------------------------------------35 5.9 Real time PCR之檢測--------------------------------------------------------------------36 第六章 討論--------------------------------------------------------------------------------------37 6.1 合成氫氧基磷灰石粉末之探討--------------------------------------------------------37 6.2 複合薄膜接枝sOGP10-14性質之探討-----------------------------------------------38 6.3 表面改質後接觸角改變量之探討-----------------------------------------------------38 6.4 MG63細胞對不同材料表面貼附行為之探討----------------------------------------39 6.5 MG63細胞對不同材料表面細胞存活率之探討-------------------------------------40 6.6 鹼性磷酸酶活性測試之探討-----------------------------------------------------------41 6.7 Real time PCR原理之探討--------------------------------------------------------------43 6.8 骨鈣素基因之表現-----------------------------------------------------------------------44 第七章 結論--------------------------------------------------------------------------------------45 第八章 未來研究目標與展望-----------------------------------------------------------------46 參考文獻-------------------------------------------------------------------------------------------47 圖 次 圖 1-a 氫氧基磷灰石粉末製程簡圖---------------------------------------------------------57 圖 1-b 氫氧基磷灰石粉末之外觀------------------------------------------------------------57 圖 2-a 刮刀(300μm、400μm、500μm、600μm)----------------------------------------------58 圖 2-b 刮膜裝置組以及所刮出600μm之薄膜---------------------------------------------58 圖 3-a PCL+HAP複合薄膜製備流程圖-----------------------------------------------------59 圖 3-b PCL+HAP複合薄膜外觀--------------------------------------------------------------59 圖 3-c 4X立體顯微鏡下PCL+HAP複合薄膜外觀-----------------------------------------60 圖 4 複合薄膜之表面改質流程圖-胜肽接枝---------------------------------------------60 圖 5-a 氫氧基磷灰石SEM圖(1000倍)------------------------------------------------------61 圖 5-b 氫氧基磷灰石SEM圖(3000倍)------------------------------------------------------61 圖 5-c 氫氧基磷灰石400℃燒結SEM圖(1000倍)----------------------------------------62 圖 5-d 氫氧基磷灰石400℃燒結SEM圖(3000倍)----------------------------------------62 圖 5-e 氫氧基磷灰石800℃燒結SEM圖(1000倍)----------------------------------------63 圖 5-f 氫氧基磷灰石800℃燒結SEM圖(3000倍)-----------------------------------------63 圖 6 氫氧基磷灰石於不同燒結溫度下XRD分析----------------------------------------64 圖 7-a 氫氧基磷灰石TEM觀察(10000倍)-------------------------------------------------65 圖 7-b 氫氧基磷灰石TEM觀察(30000倍)-------------------------------------------------65 圖 8 氫氧基磷灰石粒徑分析結果----------------------------------------------------------66 圖 9-a PCL薄膜電子顯微鏡觀察-------------------------------------------------------------67 圖 9-b PCL+HAP複合薄膜電子顯微鏡觀察-----------------------------------------------67 圖 10-a 標準PCL傅立葉轉換紅外線吸收光譜--------------------------------------------68 圖 10-b 使用傅立葉轉換紅外線吸收光譜檢測PCL薄膜--------------------------------68 圖 11-a 使用傅立葉轉換紅外線吸收光譜檢測PCL+HAP薄膜------------------------69 圖 11-b PCL薄膜與PCL+HAP薄膜傅立葉轉換紅外線吸收光譜比較----------------69 圖 12-a PCL與PCL+sOGP10-14傅立葉轉換紅外線吸收光譜比較--------------------70 圖 12-b PCL+HA與PCL+HAP+sOGP10-14傅立葉轉換紅外線吸收光譜比較------70 圖 13 薄膜材料表面接觸角測量統計圖表-----------------------------------------------71 圖 14-a MG63細胞培養12小時貼附於PCL表面之細胞型態----------------------------72 圖 14-b MG63細胞培養12小時貼附於PCL+HAP表面之細胞型態--------------------72 圖 14-c MG63細胞培養12小時貼附於PCL+aminolysis表面之細胞型態-------------73 圖 14-d MG63細胞培養12小時貼附於PCL+HAP+aminolysis表面之細胞型態-----73 圖 14-e MG63細胞培養12小時貼附於PCL+sOGP10~14表面之細胞型態-----------74 圖 14-f MG63細胞培養12小時貼附於PCL+HAP+sOGP10~14表面之細胞型態----74 圖 15-a MG63細胞培養24小時貼附於PCL表面之細胞型態----------------------------75 圖 15-b MG63細胞培養12小時貼附於PCL+HAP表面之細胞型態--------------------75 圖 15-c MG63細胞培養24小時貼附於PCL+aminolysis材料表面之細胞型態-------76 圖 15-d MG63細胞培養24小時貼附於PCL+HAP+aminolysis材料表面之細胞型態 -------------------------------------------------------------------------------------------76 圖 15-e MG63細胞培養24小時貼附於PCL+sOGP10~14表面之細胞型態-----------77 圖 15-f MG63細胞培養24小時貼附於PCL+HAP+sOGP10~14材料表面之細胞型 態----------------------------------------------------------------------------------------77 圖 16 MG63細胞於不同材料表面培養2天之MTT測試統計圖表--------------------78 圖 17 MG63細胞於不同材料表面培養4天之MTT測試統計圖表--------------------79 圖 18 MG63細胞於不同材料表面培養6天之MTT測試統計圖表--------------------80 圖 19 MG63細胞於不同材料表面4天之鹼性磷酸酶活性測試統計圖表--------81 圖 20 MG63細胞於不同材料表面10天之骨鈣素(Osteocalcin)Real time PCR相對 定量統計圖表--------------------------------------------------------------------------82 | |
dc.language.iso | zh-TW | |
dc.title | sOGP10~14 接枝於聚己內酯多元醇-氫氧基磷灰石複合薄膜對於成骨細胞活性之研究 | zh_TW |
dc.title | Effect of immobilized sOGP10-14 in polycaprolactone-hydroxyapatite composite on osteoblast activity | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 郭彥彬(Yen-Ping Kuo) | |
dc.contributor.oralexamcommittee | 賴君義(Juin-Yih Lai),王大銘(Da-Ming Wang) | |
dc.subject.keyword | 組織再生膜,聚己內酯多元醇,氫氧基磷灰石,sOGP10~14,複合薄膜,表面改質, | zh_TW |
dc.subject.keyword | Guided tissue regeneration,Polycaprolactone,Hydroxyapatite,sOGP10~14,Composite membrane,Surface modification, | en |
dc.relation.page | 82 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-09 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-96-1.pdf 目前未授權公開取用 | 3.32 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。