以非線性光學顯微術觀測骨膠原纖維排列特性

Abstract

非線性光學顯微術，相較於傳統光學的優點在於可以觀測到多光子自發螢光與倍頻等物理性質；而此類物理性質通常是傳統顯微術無法告訴我們的訊息。藉此，我們希望能夠觀測到對生物以及相關材料有意義的知識。 骨組織是人體最重要的其中一種組織，但是對於骨組織的非線性光學性質至今仍少有人提出研究。我們藉由非線性光學顯微術發現骨組織發出強烈二倍頻訊號，可看出與普通反射光不同的影像，相信對於未來研究復健科學有相當的幫助。 另外,在觀察骨組織影像時,發現裡面含有膠原蛋白纖維,而在不同的部位,排列方式有相當大的不同,因此深入研究並量測其角度改變量，發現層狀骨大部分分佈在90度以內；而織狀骨大都在90度以外，但是分佈非常廣。再以介於之中的混合骨種來檢驗，可證明我們的結論。

Nonlinear Optical Microscopy has some advantages over traditional ones. One of it is observing some physical quantity such as autofluoresce and second harmonic generation. Therefore, we would like to get some information of biology and material by the method. Bone is one of the important tissues in human body. Instead, rare studies on nonlinear optical quality of bone has been proposed. We measured that there is much second harmonic generation in bone by two-photon microscopy. Besides, by reviewing the image of bone, we knew that there is much collagen fiber inside it. However, different part, the orientation is different. Therefore, we measured the angle changes of each type to make a standard. We found that lamellar bone is largely distributed inside 90 degree; while woven bone is largely outside 90 degree. However, its distribution is much wider. Then, measuring the bones of mix kind to verify the conclusion.