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標題: | 枸杞與菊花萃取物於糖尿病眼部病變大鼠模式之影響分析 The Effects of Lycium barbarum and Chrysanthemum morifolium on Diabetic Oculopathies in Rats |
作者: | Chun-Kun Hu 胡俊琨 |
指導教授: | 林中天(Chung-Tien LLin) |
關鍵字: | 糖尿病,視網膜,白內障,枸杞,菊花, Diabetic retinopathy, Lycium barbarum,Chrysanthemum morifolium, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 糖尿病是全世界罹患人口最多的疾病之一,其所造成的併發症困擾著許多人也耗費龐大的醫療成本。對眼部方面,糖尿病視網膜病變與白內障是造成正值工作年齡之成年人後天失明最主要的原因。本研究利用streptozotocin誘導Spague-Dawley大鼠做為糖尿病眼部病變的動物模式,並投予枸杞與菊花兩種傳統中草藥為期8週,研究它們對視網膜病變以及白內障的影響。結果發現實驗期間大鼠一直維持在高血糖的狀態,糖尿病大鼠出現生長遲滯,體重隨著時間而逐漸減少,且體內醣類代謝指標糖化血紅素濃度隨時間逐漸上升。水晶體病變方面,白內障最早於糖尿病後第2週便可發現細小空泡化的形成,水晶體混濁的區域也隨時間慢慢的進展增加,病理檢查下發現水晶體除了空泡化外,還有異常的赤道區細胞增生與移行等現象。視網膜病變方面,視網膜電波圖功能之檢查發現糖尿病8週後a波與b波振幅皆顯著地下降,以及b波誘發時間延遲等情況。配合組織病理檢查發現視網膜異常的微血管突起、與感光細胞層空洞等發現,以及免疫組織化學染色所偵測到Müller cells不正常的表現glial fibrillary acidic protein (GFAP)等現象,代表糖尿病時視網膜出現功能異常與神經細胞傷害的證據。研究發現枸杞與菊花在糖尿病程的早期能減緩體重下降的速度與程度,而且枸杞能有效阻止糖尿病8週後大鼠視網膜電波圖a波與b波振幅的降低,對於神經細胞功能具有保護的效果。但是枸杞與菊花對於白內障的惡化、視網膜組織病理與糖尿病後期體重等影響皆不大。 Diabetes mellitus (DM) is a disease characterized by impaired glucose metabolism that affects all tissue in the body, including the lens and retina of the eye. Diabetic cataract and retinopathy are major ocular diseases caused by chronic hyperglycemia. In this study, streptozotocin (STZ) was intraperitoneally injected to 8 weeks old Spague-Dawley rats. Cataract formation, eletroretinography, retinal angiography and histopathology were observed and studied in the rats for a period of 8 weeks. Two Chinese herbal medicines, Lycium barbarum (Gou Qi Zi) And Chrysanthemum morifolium extract, were administrated to assess the effects of diabetic ocular complications. The cataractogenic process of lens was observed from 2nd week following streptozotocin induction, and the process was not significantly delayed by Chrysanthemum or Lycium group. Electroretinographic alternations were observed in diabetic group compared to control group 8 weeks after diabetes, the amplitudes of a- and b-waves were significantly decreased and the implicit time of b-wave was significantly delayed. However, the reductions of of a- and b-wave amplitudes were not observed in the Lycium group. Histopathological study and retinal angiography were also examined for morphologic changes and neovascularization, but no significant differences between herb-treated groups and un-treated group in diabetic rats. The results suggested that Lycium has a protective role in diabetic retinopathy. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29878 |
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顯示於系所單位: | 獸醫學系 |
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