請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29669完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 周宏農 | |
| dc.contributor.author | Jain-Shin Wu | en |
| dc.contributor.author | 吳建欣 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:14:16Z | - |
| dc.date.available | 2007-07-26 | |
| dc.date.copyright | 2007-07-26 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-20 | |
| dc.identifier.citation | 周宏農，1999。水產品藻源毒素檢測操作手冊，行政院衛生署。
盧重光，2001。台灣產兩種原甲藻之毒素研究。國立台灣大學海洋研究所，博士論文。 余昌憲，2006。利用原甲藻磷營養鹽供應模式對生長及大環內脂天然物生產的影響。國立台灣大學漁業科學研究所，碩士論文。 吳建民、楊中林，2007。大孔樹脂純化山楂葉總黃酮和金絲桃苷工藝研究。海峽藥學。19, 53-55. 雷玉霞、張長弓、趙麗、鐘巧妮、程明、付琴琴，2007。大孔吸附樹脂分離純化紅花黃色素的工藝研究。醫藥導報。26, 656-658 趙麗、張長弓、鐘巧妮、雷玉霞、程明、王仲，2007。大孔吸附樹脂分離純化五鶴續斷總皂苷的工藝研究。醫藥導報。26, 649-651 Akiyama, T., Harada, S., Kojima, F., Takahshi, Y., Imada, C., Okami, T., Muraoka, Y., Aoyagi, T. and Takeuchi, T., 1998, Fluostatins A and B, New Inhibitors of Dipeptidyl Peptidase Ⅲ, Produced by Streptomyces sp. TA-3391. J. Antibiot. 51, 553-559. Awa, Y., Iwai, N., Ueda, T., Suzuki, K., Asano, S., Yamagishi, J., Nagai, K., Wachi, M., 2005, Isolation of a new antibiotic, Alaremycin, structurally related to 5-aminolevulinic acid from Streptomyces sp. A012304. Biosci. Biotechnol. Biochem. 69, 1721-1725. Bravo, I., Fernandez, M. L., Ramilo, I. and Martinez, A., 2001, Toxin composition of the toxic dinoflagellate Prorocentrum lima isolated from different locations along the Galician coast (NW Spain). Toxicon 39, 1537-1545. Cavaco, S. A., Fernandes, S., Q, M. M. and Ferreira, L. M., 2007, Removal of chromium from electroplating industry effluents by ion exchange resins. J. Harard Mater. 144, 634-638. Cohen, P., Holmes, C.F.B. and Tsukitani, Y., 1990, Okadaic acid: a new probe for the study of cellular regulation. Trend Biochem. Sci. 15, 98-102. Daranas, H., Norte, M. and Ferna´ndez, J.J., 2001, Toxic marine microalgae. Toxicon 39, 1101–1132. Fernandez, M. L., Miguez, A., Cacho., E. and Martinez, A., 1996, Detection of okadaic acid esters in the hexane extracts of spanish mussels. Toxicon 34, 381-387. Hallegraeff, G. M., Anderson, D. M., Cembella, A. D. and Enevoldsen, 1995, Manual on Harmful Algal Bloom. Intergovernmental Oceanographic Commision (of UNESCO) Manuals and guides 33. Heredia-Tapia, A., Arredondo-Vega, B. O., Nuñez-Vázquez,E. J., Yasumoto, T., Yasuda, M. and Ochoa, J. L., 2002, Isolation of Prorocentrum lima (Syn. Exuviaella lima) and diarrhetic shellfish poisoning (DSP) risk assessment in the Gulf of California, Mexico. Toxicon 40,1121-1127 Hu, T., Burton, I., Curtis, J. M., Quilliam, M. A., Walter, J. A., Windust, A. J. and Wright, J. L.C., 1999, Oxidative Transformation of a Naturally Occurring Okadaic Acid Diol Ester by the Diatom Thalassiosira weissflogii. Tetrahedron Lett. 40, 3981-3984 Hu, T., Curtis, J. M., Walter, J. A. and Wright, J. L. C., 1995a, Identification of DTX-4, a new water-soluble phosphatese inhibitor from the toxic dinoflagellate Prorocentrum lima, J. Chem. Soc. 597-599. Hu, T., Curtis, J. M., Walter, J. A. McLachlan, J. L. and Wright, J. L. C., 1995b, Two new water-soluble DPS toxin deriveatives from dinoflagellate Prorocentrum maculasum: possible storage and excreteon products, Tetrahedron Lett. 36, 9273-9275. Hu, T., deFreitas, A. S. W., Doyle, J., Jackson, D., Marr, J., Nixon, E., Pleasance, S., Quilliam, M. A., Walter, J. A. and Wright, J. L. C., 1993, New DSP toxin derivatives isolated from toxin mussels and the dinoflagellates, Prorocentrum lima and Prorocentrum concavum, In Toxic Phytoplankton Blooms in the Sea, Smayda and Shimiza, Eds., Elsevier pp. 507-511. Hu, T., Marr, J., deF reitas, A. S. W., Quilliam, M. A., Walter, J. A. and Wright, J. L. C., 1992, New diol esters isolated from cultures of the dinoflagellates Prorocentrum lima and Prorocentrum concavum, J. Nat. Prod. 55, 1631-1637. Kumagai, M., Yanagi, T., Murata, M., Yasumoto, Y. and Kat, M., 1986, Okadaic acid as the causative toxin of diarrhetic shellfish poisoning in Europe. Agric. Biol. Chem. 50, 2853–2857. Ichiyama, K., Tai, A. and Yamamoto, I., 2007, Augmentation of antigen-specific antibody production and IL-10 generation with a fraction from Rooibos (Aspalathus linearis) tea. Biosci. Biotechnol. Biochem. 71, 598-602. Inoue, T., Sugimoto, Y., Masuda, H. and Kamei, C., 2001, Effects of peppermint (Mentha piperita L.) extracts on experimental allergic rhinitis in rats. Bio. Pharm. Bull. 24, 92-95. Itoh, T., Umekawa, H. and Furuichi, Y., 2005, Potential ability of hot water adzuki (Vigna angularis) extracts to inhibit the adhesion, invasion, and metastasis of murine B16 melanoma cells. Biosci. Biotechnol. Biochem. 69, 448-454. Kuo, C. C., Chiang, W., Liu, G. P., Chien, Y. L., Chang, J. Y., Lee, C. K., Lo, J. M., Huang, S. L., Shih, M. C. and Kuo, Y. H., 2002, 2,2'-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. J. Aqric. Food. Chem. 50, 5850-5855. Lee, J. S., Yanagi, T., Kenma, R. and Yasumoto, T., 1987, Fluorimetric determination of diarrhetic shellfish toxins by high-performance liquid chromatography. Agric. Biol. Chem. 51, 877-881. Lee, J.C., Park, H.R., Park, D.J., Lee, H.B., Kim, Y.B., Kim, C.J., 2003, Improved production of teicoplanin using adsorbent resin in fermentations. Lett. in Appl. Microbio. 37, 196-200. Momose, I., Kinoshita, N., Sawa, R., Naganawa, H., Iinuma, H., Hamada, M. and Takeuchi, T., 1997, Nothramicin, a New Anthracycline Antibiotic from Nocardia sp. MJ896-43F17. J. Antibiot. 51, 130-135. Murakami, Y., Oshima, Y. and Yasumoto, T., 1982, Identification of okadaic acid as a toxic component of a marine dinoflagellate Prorocentrum lima. Nippon Suisan Gakkaishi 48, 69-72. Okada, H., Kamiya, S., Shina, Y., Suwa, H., Nagashima, M., Nakajima, S., Shimokawa, H., Sugiyama, E., Kondo, H., Kojiri, K. and Suda, H., 1998, BE-31405, a New Antifungal Antibiotic Produced Penicillium minioluteum. J. antibiot. 51, 1081-1086. Quilham, M. A., Hardstaff, W. R., Ishida, N., McLachlan, J. L., Reeves, A. R., Ross, N. W. and Windust, A. J., 1996, Production of diarrhetic shellfish poisoning (DSP) toxins by Prorocentrum lima in culture and development of analytical methods. Harntful and Toxic Algal Bloom 289-292. Roja, G., Bhangale, A. S., Juvekar, A. R., Eapen, S.and D'Souza, S. F., 2005, Enhanced production of the polysaccharide arabinogalactan using immobilized cultures of Tinospora cordifolia by elicitation and in situ adsorption. Biotechnol. Proq. 21, 1788-1691. Song, M. J., Bae, J., Lee, D. S., Kim, C. H., Kim, J. S., Kim, S. W. and Hong, S. I., 2006, Purification and characterization of prodigiosin produced by integarated bioreactor from Serratua sp. KH-95. J. Biosci. Bioend. 101, 157-161. Suarez-Gomez, B., Souto, M. L., Norte, M., and Fernandez, J. J., 2001, Isolation and Structural Determination of DTX-6, a New Okadaic Acid Derivative. J. Nat. Prod. 64, 1363-1364 Ten-Hage, L., Delaunay, N., Pichon, V., Coute, A., Puiseux-Dao, S. and Turquet, J., 2000, Okadaic acid production from the marine benthic dinoflagellate Prorocentrum arenarium Faust (Dinophyceae) isolated from Europa Island coral reef ecosystem (SW Indian Ocean). Toxicon 38, 1043-1054. Takatsuji, W., Nakauchi, M. and Yoshida, H., 1999, Removal of Salt and Organic Acids from Solution Used to Season Salted Japanese Apricots (Ume) by Electrodialysis, Precipitation and Adsorption. J. Biosci. Bioeng. 88, 348-351. Tuzen, M., Uluozlu, O. D. and Soylak, M., 2006, Cr(VI) and Cr(III) speciation on Bacillus sphaericus loaded diaion SP-850 resin. J. Hazard Mater. 144, 549-555. Vale, P. Antonia, M. and Sampayo, M., 2002, Esterification of DSP toxin by Portuguese bivalves from the Northwest coast determined by LC-MS, a widespread phenomenon. Toxicon 40, 33-42 Windust, A. J., Quilliam, M. A., Wright, J. L. C. and McLachlan, J. L., 1997, Comparative toxicity of the diarrhetic shellfish poisons, okadaic acid, okadaic acid diol ester and dinophysistoxin-4 to the diatom Thalassiosira weissflogii. Toxicin 35, 1591-1603. Windust, A. J., Wright, J. L. C. and McLachlan, J. L., 1996, The effects of the diarrhetic shellfish poisoning toxins, okadaic acid and dinophysistoxin-I, on the growth of microalgae. Mar. Biol.126, 19-25. Wright, J. L. C., 1995, Dealing with seafood toxin: present approaches and future options, Food. Res. Int. 28, 347-358 Yang, W. B., Li, A., Fan, J., Yang, L. and Zhang, Q., 2006, Adsorption of branched alkylbenzene sulfonate onto styrene and acrylic ester resins. Chemophere 64, 984-990. Yan, P. S., Yuan, S., Sakuno, E., Nakajima, H., Nakagawa, H. and Yabe, K., 2004, Cyclo(L-leucyl-L-prolyl) produced by Achromobacter xylosoxidans inhibits aflatoxin production by Aspergillus parasiticus. Appl. Environ. Microbiol. 70, 7466-73 Yasumoto T., Murata, M., Oshima, Y., Sano, M., Matsumoto, G. K. and Clardy, J., 1985, Diarrhetic shellfish toxins, Tetrahedron, 41, 1019 -1025. Uyeda, M., Yokomizo, K., Miyamoto, Y. and Habib, E., 1998, Fattiviracin A1, a Novel Antiherpetic Agent Peoduced by Streptomyces microflavus Strain No. 2445. J. Antibiot. 51, 823-828. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29669 | - |
| dc.description.abstract | 本研究探討黑海綿酸的提純最適化，在研究初期發現藻細胞在培養至穩定期後，釋放黑海綿酸到培養液中的濃度大量提高，不利於從藻細胞內提純黑海綿酸，此時針對培養液中黑海綿酸的提純，較從藻細胞後內提純更簡單，因此本研究以合成吸附樹脂SP825吸附培養液中的黑海綿酸後，再行脫附提純。SP825對黑海綿酸吸附實驗中，發現SP825吸附承載量至少為4.736 mg/g，而SP825對黑海綿酸的脫附率可達96.9 %，整個操作不僅能完全吸附培養基中的黑海綿酸，並可利用甲醇來達到脫附毒素的目的，增加量化生產時濃縮操作的方便性，顯示SP825可用於黑海綿酸吸附萃取；接著以二氯甲烷進行分配萃取，並將二氯甲烷層進行矽膠速分管柱層析，利用正己烷與異戊烷為沖提液，並以梯度方式進行沖提，合併含毒素分液後，以製備型高效液相層析更進一步純化毒素，最後能獲得95%純度的黑海綿酸。
利用SP825茶包與藻細胞培養實驗中，發現SP825茶包組黑海綿酸釋放量遠低於控制組，推測高濃度黑海綿酸對藻細胞是一種生存環境壓力，造成藻細胞本身釋放更多黑海綿酸。另外，本實驗分析17株利瑪原甲藻藻株間的黑海綿酸含量，發現不同藻株之間與藻細胞內外黑海綿酸含量皆有差異，以#1a為黑海綿酸生產之最適藻株，其單位細胞總產毒量47.2 pg/cell為最高。 | zh_TW |
| dc.description.abstract | This research utilize the release of okadaic acid in Prorocentrum lima to probe the best purification method of okadaic acid. When growing to the steady phase in experiment, cells begin to release okadaic acid to media fast which makes better purificaiton than directly from cell. This research utilize SP825 resin absorb okadaic acid in media, and then purify it. In the experiment of okadaic acid content analysis among different Prorocentrum lima , the differences of okadaic acid content are among Prorocentrum lima strains, and cell, and media. The strains #1a, #2 and #15 have relatively high okadaic acid content per cell. And SP825 resin adsorbability of okadaic acid is 4.736 mg / g at least, however SP825 resin deadsorbent rate can be up to 96.9%. To extract okadaic acid using dichloromethane partition SP825 methanol eluent, before running silica gel flash column. The mobile phase are hexane and isopropanol. After combining toxin dichloromethane fraction, it’s reverse phase high performance liquid chromatography purification and then we can get 95% pure okadaic acid. From deadsorption okadaic acid with SP825 to purification, it’s certainly easier procedures and less time and money consuming. .
In the experiment of P. lima culture with SP825 teabag, the result shows the okadaic acid SP825 teabag group producing is far less than control group. The author infers that okadaic acid is a key factor as environmental pressure of P. lima. When okadaic acid in media increases, it results in P. lima releasing more okadaic acid instinctively. Besides, in the experiment of okadaic acid content analysis among different Prorocentrum lima , the differences of okadaic acid content are among Prorocentrum lima strains, and cell, and media. The strains #1a, #2 and #15 have relatively high okadaic acid content per cell suiting okadaic acid purification. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:14:16Z (GMT). No. of bitstreams: 1 ntu-96-R94b45018-1.pdf: 1190296 bytes, checksum: 0ed3ec1bafc15c973e5aecd7bf4ea81b (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 中文摘要 i
英文摘要 ii 壹、前言 1 貳、研究方法與材料 8 一、 藻株與培養條件 8 二、 合成吸附樹脂SP825 8 三、 培養液中黑海綿酸的分析 9 四、 合成吸附樹脂吸附量與脫附率的計算 11 五、 實驗項目 11 1. 利瑪原甲藻藻株的黑海綿含量分析 11 2. 不同培養條件對於利瑪原甲藻培養液中黑海綿酸含量影響 12 3. 利瑪原甲藻的生長與毒素釋放 12 4. SP825對黑海綿酸純質的脫附率 13 5. 沖提方式對SP825中黑海綿酸的脫附率 14 5-1 batch式沖提 14 5-2 flow式沖提 15 6. SP825最大吸附量 16 7. 利瑪原甲藻與SP825茶包培養 17 8. 黑海綿酸提純 18 8-1 SP825沖提液的選擇 18 8-2 自動化快速層析系統 (CombiFlash Companion) 19 第三章 結果 20 1. 利瑪原甲藻藻株的黑海綿含量分析 20 3. 利瑪原甲藻的生長與毒素釋放關係 20 4. SP825對黑海綿酸純質的脫附率 21 5. 沖提方式對SP825中黑海綿酸的脫附率 21 5-1 batch式沖提 22 5-2 flow式沖提 22 6. SP825吸附量測試 22 7. 利瑪原甲藻與SP825茶包培養 23 8. 黑海綿酸提純最佳化 24 8-1 SP825沖提液的選擇 24 8-2 自動化快速層析系統 (CombiFlash Companion) 24 第四章 討論 26 一、利瑪原甲藻藻株的黑海綿酸毒素產量分析 26 二、利瑪原甲藻釋放黑海綿酸與SP825萃取黑海綿酸的模式 26 三、SP825樹脂應用於黑海綿酸提純 29 四、黑海綿酸提純最佳化 30 第六章、參考文獻 33 | |
| dc.language.iso | zh-TW | |
| dc.subject | 吸附樹脂 | zh_TW |
| dc.subject | 渦鞭毛藻 | zh_TW |
| dc.subject | 利瑪原甲藻 | zh_TW |
| dc.subject | 黑海綿酸 | zh_TW |
| dc.subject | 毒素釋放 | zh_TW |
| dc.subject | Okadaic acid | en |
| dc.subject | Toxin release | en |
| dc.subject | Diaion SP825 | en |
| dc.subject | Peoeocentrum lima | en |
| dc.title | 利瑪原甲藻的黑海綿酸釋放與提純最佳化研究 | zh_TW |
| dc.title | Studies on the release of okadaic acid in Prorocentrum lima and the methodological optimization of okadaic acid purification | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃穰,李宗徽 | |
| dc.subject.keyword | 渦鞭毛藻,,利瑪原甲藻,,黑海綿酸,吸附樹脂,毒素釋放, | zh_TW |
| dc.subject.keyword | Peoeocentrum lima,Okadaic acid,Diaion SP825,Toxin release, | en |
| dc.relation.page | 56 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 1.16 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。