Calneuron調控鈣離子訊息傳遞的研究

Abstract

Calneuron具有兩個可以和鈣離子結合的EF-hand motif，且在其C端有一段疏水性的結構。本論文中，我們利用PCR技術選殖出大鼠的calneuron，並將其表現在HEK293t細胞中，發現分布位置主要是在細胞膜附近；而缺乏疏水性C-端的變異（Cal

Calneuron has a structure similar to calmodulin-like proteins but has only two conserved N-terminal EF-hand motifs and an extent C-terminal hydrophobic tail. The expression of calneuron was specific in neuronal tissues and higher in adult than in fetal rat cortex. When expressed in HEK293t cells, calneuron was localized at the plasma membrane, but changed to the cytosol for the mutant without the C-terminal hydrophobic tail (Cal