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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29345完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 馬一 中(Yee-Chung Ma) | |
| dc.contributor.author | Pei-Lin Kao | en |
| dc.contributor.author | 高佩鈴 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:04:58Z | - |
| dc.date.available | 2009-08-08 | |
| dc.date.copyright | 2007-08-08 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-24 | |
| dc.identifier.citation | 1.Phillips M, Herrera J, Krishnan S, Zain M, Greenberg J, Cataneo RN. Variation in volatile organic compounds in the breath of normal humans. Journal of Chromatography B 1999;729(1-2):75-88.
2.Wu LL, Chiou CC, Chang PY, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clinica Chimica Acta 2004;339(1-2):1-9. 3.Wyse CA, Preston T, Yam PS, Sutton DGM, Christley RM, Hotchkiss JW, Mills CA, Glidle A, Cumming DRS, Cooper JM, Love S. Current and future uses of breath analysis as a diagnostic tool. Veterinary Record 2004;154(12):353-360. 4.Huang MF, Lin WL, Ma YC. A study of reactive oxygen species in mainstream of cigarette. Indoor Air 2005;15(2):135-140. 5.林冠宇. 偵測呼出氣體中所含揮發性有機物作為指標在環境衛生上的應用－可行性的探討. 國立台灣大學公共衛生學院環境衛生研究所碩士論文 2004. 6.Ashok BT, Ali R. The aging paradox: free radical theory of aging. Experimental Gerontology 1999;34(3):293-303. 7.Cao WQ, Duan YX. Breath analysis: Potential for clinical diagnosis and exposure assessment. Clinical Chemistry 2006;52(5):800-811. 8.Carpagnano GE, Kharitonov SA, Foschino-Barbaro MP, Resta O, Gramiccioni E, Barnes PJ. Increased inflammatory markers in the exhaled breath condensate of cigarette smokers. European Respiratory Journal 2003;21(4):589-93. 9.Wyse CA, Preston T, Yam PS, Sutton DG, Christley RM, Hotchkiss JW, Mills CA, Glidle A, Cumming DR, Cooper JM, Love S. Current and future uses of breath analysis as a diagnostic tool. Veterinary Record 2004;154(12):353-60. 10.Pleil JD, Lindstrom AB. Exhaled human breath measurement method for assessing exposure to halogenated volatile organic compounds. Clinical Chemistry 1997;43(5):723-730. 11.Miekisch W, Schubert JK, Noeldge-Schomburg GF. Diagnostic potential of breath analysis--focus on volatile organic compounds. Clinica Chimica Acta 2004;347(1-2):25-39. 12.Aghdassi E, Allard JP. Breath alkanes as a marker of oxidative stress in different clinical conditions. Free Radical Biology & Medicine 2000;28(6):880-6. 13.Phillips M, Cataneo RN, Greenberg J, Grodman R, Salazar M. Breath markers of oxidative stress in patients with unstable angina. Heart Disease 2003;5(2):95-9. 14.Turner C, Spanel P, Smith D. A longitudinal study of breath isoprene in healthy volunteers using selected ion flow tube mass spectrometry (SIFT-MS). Physiological Measurement 2006;27(1):13-22. 15.Nelson N, Lagesson V, Nosratabadi AR, Ludvigsson J, Tagesson C. Exhaled isoprene and acetone in newborn infants and in children with diabetes mellitus. Pediatric Research 1998;44(3):363-367. 16.Scislowski PWD, Pickard K. The Regulation of Transaminative Flux of Methionine in Rat-Liver Mitochondria. Archives of Biochemistry and Biophysics 1994;314(2):412-416. 17.Scholpp J, Schubert JK, Miekisch W, Geiger K. Breath markers and soluble lipid peroxidation markers in critically ill patients. Clinical Chemistry and Laboratory Medicine 2002;40(6):587-594. 18.Grote C, Pawliszyn J. Solid-phase microextraction for the analysis of human breath. Analytical Chemistry 1997;69(4):587-596. 19.Schubert JK, Spittler KH, Braun G, Geiger K, Guttmann J. CO2-controlled sampling of alveolar gas in mechanically ventilated patients. Journal of Applied Physiology 2001;90(2):486-492. 20.Phillips M. Method for the collection and assay of volatile organic compounds in breath. Analytical Biochemistry 1997;247(2):272-278. 21.Risby TH, Sehnert SS. Clinical application of breath biomarkers of oxidative stress status. Free Radical Biology and Medicine 1999;27(11-12):1182-1192. 22.Machado RF, Laskowski D, Deffenderfer O, Burch T, Zheng S, Mazzone PJ, Mekhail T, Jennings C, Stoller JK, Pyle J, Duncan J, Dweik RA, Erzurum SC. Detection of lung cancer by sensor array analyses of exhaled breath. American Journal of Respiratory & Critical Care Medicine 2005;171(11):1286-91. 23.Kharitonov SA, Barnes PJ. Exhaled biomarkers. Chest 2006;130(5):1541-1546. 24.Hamid Q, Springall DR, Riverosmoreno V, Chanez P, Howarth P, Redington A, Bousquet J, Godard P, Holgate S, Polak JM. Induction of Nitric-Oxide Synthase in Asthma. Lancet 1993;342(8886-7):1510-1513. 25.Wechsler ME, Grasemann H, Deykin A, Silverman EK, Yandava CN, Israel E, Wang M, Drazen JM. Exhaled nitric oxide in patients with asthma - Association with NOS1 genotype. American Journal of Respiratory and Critical Care Medicine 2000;162(6):2043-2047. 26.Dupont LJ, Demedts MG, Verleden GM. Prospective evaluation of the validity of exhaled nitric oxide for the diagnosis of asthma. Chest 2003;123(3):751-756. 27.Ekroos H, Tuominen J, Sovijarvi ARA. Exhaled nitric oxide and its long-term variation in healthy non-smoking subjects. Clinical Physiology 2000;20(6):434-439. 28.Maziak W, Loukides S, Culpitt S, Sullivan P, Kharitonov SA, Barnes PJ. Exhaled nitric oxide in chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine 1998;157(3):998-1002. 29.Kharitonov SA, Barnes PJ. Biomarkers of some pulmonary diseases in exhaled breath. Biomarkers 2002;7(1):1-32. 30.Phillips M, Gleeson K, Hughes JMB, Greenberg J, Cataneo RN, Baker L, McVay WP. Volatile organic compounds in breath as markers of lung cancer: a cross-sectional study. Lancet 1999;353(9168):1930-1933. 31.Phillips M, Cataneo RN, Cummin AR, Gagliardi AJ, Gleeson K, Greenberg J, Maxfield RA, Rom WN. Detection of lung cancer with volatile markers in the breath.[see comment]. Chest 2003;123(6):2115-23. 32.Poli D, Carbognani P, Corradi M, Goldoni M, Acampa O, Balbi B, Bianchi L, Rusca M, Mutti A. Exhaled volatile organic compounds in patients with non-small cell lung cancer: cross sectional and nested short-term follow-up study. Respiratory Research 2005;6:71. 33.Olopade CO, Zakkar M, Swedler WI, Rubinstein I. Exhaled pentane levels in acute asthma. Chest 1997;111(4):862-865. 34.Christon JA, Zakkar M, Stepanski EJ, Swedler WI, Basner RC, Olopade CO, Rubinstein I. Exhaled Pentane Levels in Patients with Obstructive Sleep-Apnea. Faseb Journal 1995;9(3):A375-a375. 35.Phillips M CR, Greenberg J, Grodman R, Gunawardena R, Naidu A. Effect of oxygen on breath markers of oxidative stress. European Respiratory Journal 2003;21(1):48-51. 36.Schubert JK, Miekisch W, Geiger K, Noldge-Schomburg GF. Breath analysis in critically ill patients: potential and limitations. Expert Review of Molecular Diagnostics 2004;4(5):619-29. 37.Knutson MD, Handelman GJ, Viteri FE. Methods for measuring ethane and pentane in expired air from rats and humans. Free Radical Biology & Medicine 2000;28(4):514-9. 38.Scholpp J, Schubert JK, Miekisch W, Geiger K. Breath markers and soluble lipid peroxidation markers in critically ill patients. Clinical Chemistry & Laboratory Medicine 2002;40(6):587-94. 39.Moretti M, Phillips M, Abouzeid A, Cataneo RN, Greenberg J. Increased breath markers of oxidative stress in normal pregnancy and in preeclampsia.[see comment]. American Journal of Obstetrics & Gynecology 2004;190(5):1184-90. 40.Phillips M, Cataneo RN, Greenberg J, Gunawardena R, Rahbari-Oskoui F. Increased oxidative stress in younger as well as in older humans.[see comment]. Clinica Chimica Acta 2003;328(1-2):83-6. 41.Phillips M, Greenberg J, Cataneo RN. Effect of age on the profile of alkanes in normal human breath. Free Radical Research 2000;33(1):57-63. 42.Tsuboi H, Kouda K, Takeuchi H, Takigawa M, Masamoto Y, Takeuchi M, Ochi H. 8-hydroxydeoxyguanosine in urine as an index of oxidative damage to DNA in the evaluation of atopic dermatitis. British Journal of Dermatology 1998;138(6):1033-5. 43.Ou BX, Huang DJ. Fluorescent approach to quantitation of reactive oxygen species in mainstream cigarette smoke. Analytical Chemistry 2006;78(9):3097-3103. 44.Euler DE, Dave SJ, Guo H. Effect of cigarette smoking on pentane excretion in alveolar breath. Clinical Chemistry 1996;42(2):303-8. 45.Poli D, Carbognani P, Corradi M, Goldoni M, Acampa O, Balbi B, Bianchi L, Rusca M, Mutti A. Exhaled volatile organic compounds in patients with non-small cell lung cancer: cross sectional and nested short-term follow-up study. Respiratory Research 2005;6. 46.Loft S, Vistisen K, Ewertz M, Tjonneland A, Overvad K, Poulsen HE. Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index. Carcinogenesis 1992;13(12):2241-7. 47.Prieme H, Loft S, Klarlund M, Gronbaek K, Tonnesen P, Poulsen HE. Effect of smoking cessation on oxidative DNA modification estimated by 8-oxo-7,8-dihydro-2 '-deoxyguanosine excretion. Carcinogenesis 1998;19(2):347-351. 48.Allard JP, Royall D, Kurian R, Muggli R, Jeejeebhoy KN. Effects of beta-carotene supplementation on lipid peroxidation in humans. American Journal of Clinical Nutrition 1994;59(4):884-90. 49.Hoshino E, Shariff R, Van Gossum A, Allard JP, Pichard C, Kurian R, Jeejeebhoy KN. Vitamin E suppresses increased lipid peroxidation in cigarette smokers. Jpen: Journal of Parenteral & Enteral Nutrition 1990;14(3):300-5. 50.Hatzinikolaou DG, Lagesson V, Stavridou AJ, Pouli AE, Lagesson-Andrasko L, Stavrides JC. Analysis of the gas phase of cigarette smoke by gas chromatography coupled with UV-diode array detection. Analytical Chemistry 2006;78(13):4509-4516. 51.Jo WK, Pack KW. Utilization of breath analysis for exposure estimates of benzene associated with active smoking. Environmental Research 2000;83(2):180-7. 52.Kalaitzoglou M, Samara C. Gas/particle partitioning and yield levels of polycyclic aromatic hydrocarbons and n-alkanes in the mainstream cigarette smoke of commercial cigarette brands. Food And Chemical Toxicology 2006;44(8):1432-1442. 53.Brugnone F, Perbellini L, Maranelli G, Romeo L, Guglielmi G, Lombardini F. Reference values for blood benzene in the occupationally unexposed general population. International Archives of Occupational & Environmental Health 1992;64(3):179-84. 54.Kanaya S, Ikeya M, Yamamoto K, Moriya T, Furuhashi K, Sonoda M, Goto K, Ochi H. Comparison of an oxidative stress biomarker 'urinary8-hydroxy-2'-deoxyguanosine,' between smokers and non-smokers. Biofactors 2004;22(1-4):255-8. 55.Chuang CY, Lee CC, Chang YK, Sung FC. Oxidative DNA damage estimated by urinary 8-hydroxydeoxyguanosine: influence of taxi driving, smoking and areca chewing. Chemosphere 2003;52(7):1163-71. 56.Loft S, Vistisen K, Ewertz M, Tjonneland A, Overvad K, HE P. Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in humans: inXuence of smoking, gender and body mass index. Carcinogenesis 1992;13:2241-2247. 57.Pilger A, Rudiger HW. 8-Hydroxy-2 '-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures. International Archives Of Occupational And Environmental Health 2006;80(1):1-15. 58.Gordon SM, Wallace LA, Brinkman MC, Callahan PJ, Kenny DV. Volatile organic compounds as breath biomarkers for active and passive smoking. Environmental Health Perspectives 2002;110(7):689-98. 59.Sakamoto W, Isomura H, Fujie K, Nishihira J, Ozaki M, Yukawa S. Coffee increases levels of urinary 8-hydroxydeoxyguanosine in rats. Toxicology 2003;183(1-3):255-63. 60.Kim JY, Mukherjee S, Ngo LC, Christiani DC. Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates. Environmental Health Perspectives 2004;112(6):666-71. 61.Meagher EA, FitzGerald GA. Indices of lipid peroxidation in vivo: strengths and limitations. Free Radical Biology & Medicine 2000;28(12):1745-50. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29345 | - |
| dc.description.abstract | 呼氣分析為非侵入性之生物偵測方法，呼出氣體中戊烷被視為氧化壓力指標如同尿液中8-hydroxy-2’-deoxyguanosine (8-OHdG)。抽菸會明顯增加人體氧化壓力，因此本研究以年長抽菸者為對象，觀察抽菸對呼出氣體揮發性有機物和尿液中8-OHdG的影響。
本研究為縱貫式方法，收集受試者（8位抽菸者,8位非抽菸者）呼出氣體和尿液樣本每天早上一次，並紀錄抽菸者每天的抽菸量、上次抽菸的時間、喝酒習慣、服用維他命習慣。另外針對抽菸者採集長時間禁菸下（至少7小時）的呼出氣體。呼氣樣本用採樣袋收集，以複合床熱脫附採樣管來濃縮呼出氣體中揮發性有機物，運用熱脫附儀配合氣相層析質譜儀來進行分析。尿液樣本以酵素免疫吸附分析8-OHdG濃度。統計方面採用混合型模式(Mixed-effect model)校正固定效應和隨機效應後，比較抽菸者和非抽菸者呼氣中揮發性有機物的差異。 結果顯示呼出氣體中揮發性有機物的含量於抽菸後快速上升，並受到年齡和服用維他命習慣的影響。利用模式校正這些干擾因子後，在65個VOCs中有26個物質在抽菸組和非抽菸組有顯著差異。抽菸組長時間禁菸下和非抽菸組的呼氣VOCs比較也有類似結果，並觀察到抽菸者呼氣中pentane含量依然比非抽菸者高。尿液中8-OHdG方面，抽菸組的含量比非抽菸組高出1.5倍，且和呼出氣體中戊烷有相關(r=0.55, p=0.06, n=12)。 呼出氣體VOCs的含量於抽菸後馬上增加並受到年齡和使用抗氧化物情形的影響。呼出氣體中戊烷和尿液中8-OHdG一樣作為氧化壓力的指標，其年長抽菸者含量都比非抽菸者來得高。 | zh_TW |
| dc.description.abstract | Breath analysis is a non-invasive biological monitoring method. Exhaled air pentane has been used as a marker of oxidative stress as well as urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG). Because cigarette smoking can significantly increase the oxidative stress, this study used older smokers as the subjects and observed the effect of smoking on exhaled breath volatile organic compounds (VOCs) and 8-OHdG in urine.
A longitudinal study of the subjects （8 smokers, 8 non-smokers）was carried out with exhaled breath and urine sampling once every morning and recorded smokers’ daily cigarette consumption, time of the last smoking、alcohol intake and vitamin supplement. In addition, in smokers, exhaled breath was collected after cigarette cessation. Breath VOCs samples in Tedlar bags were preconcentrated with multi-bed sorbent sampling tubes and analyzed by thermal desorption-GC/MS. Urinary 8-OHdG was measured by ELISA. Mixed-effect models were used to investigate the breath VOCs difference between smokers and non-smokers by adjusting fixed effects and random effects. We observed that exhaled breath VOCs increased immediately after smoking and were influenced by age and vitamin consumption. Using model adjusting these factors, 26 of 65 breath VOCs showed significant difference between smokers and non-smokers. Similar results were measured in smokers after cigarette cessation compared to non-smokers. Exhaled breath pentane in older smokers was still higher than non-smokers. In urinary 8-OHdG, smokers had 1.5-fold higher level than non-smokers. There was a correlation between exhaled breath pentane and urinary 8-OHdG in smokers.（r=0.55, p=0.06, n=12）. In conclusion, cigarette smoking caused an immediate increase in exhaled breath VOCs which were also influenced by age and antioxidant supplement. Exhaled breath pentane as well as urinary 8-OHdG were used as a marker of oxidative stress and were increased in older smokers compared to non-smokers. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:04:58Z (GMT). No. of bitstreams: 1 ntu-96-R94844010-1.pdf: 765189 bytes, checksum: 779ec36851535438b6709aa2204f37cb (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 摘要 1
Abstract 2 第一章 緒論 3 1.1 前言 3 1.2 研究目的 4 第二章 文獻探討 5 2.1 呼出氣體之研究 5 2.1.1 呼氣分析研究種類 5 2.1.2 呼氣中VOCs之來源 6 2.1.3呼氣檢測之特性 8 2.1.4呼氣採集方法 8 2.1.5 呼出氣體分析作為肺部疾病之生物指標 9 2.2 氧化壓力指標 10 2.2.1 氧化壓力 10 2.2.2 呼出氣體中氧化壓力指標 11 2.2.3 尿液中氧化壓力指標 11 2.3 抽菸 12 2.3.1 抽菸對氧化壓力的影響 12 2.3.2 抽菸對呼出氣體的影響 13 2.3.3 抽菸對尿液8-OHdG的影響 13 第三章 材料與方法 14 3.1 研究流程 14 3.2 研究設計 14 3.3 研究對象 15 3.4採樣方法 15 3.5 呼氣實驗器材與設備 15 3.5.1藥品、試劑及氣體 15 3.5.2呼氣實驗儀器 17 3.6呼氣樣本前處理 17 3.6.1採樣管之填充 17 3.6.2樣本保存 18 3.6.3採樣管調態(Conditioning) 18 3.6.4採樣管流量校正 18 3.6.5樣本前濃縮步驟 18 3.6.6採樣袋淨化 19 3.7呼氣分析步驟 19 3.7.1熱脫附儀上機前調態 19 3.7.2氣相層析質譜儀 19 3.7.3上機分析 20 3.8儀器分析條件 20 3.8.1熱脫附裝置 20 3.8.2低溫捕集器(Cryo-Focus Module, CFM) 21 3.8.3氣相層析儀 21 3.8.4質譜儀 22 3.9空白樣品分析 22 3.10重複樣品分析 23 3.11內標準品監測 23 3.12破出試驗 23 3.13定性、定量分析 23 3.14 尿液中8-OHdG 25 3.14.1 ELISA Kit 25 3.14.2 Kit內容物 25 3.14.3 分析步驟 25 3.15 統計分析 26 第四章 結果 27 4.1 QA/QC 27 4.1.1 空白試驗 27 4.1.3 破出實驗 27 4.1.2 重覆樣本 27 4.2 定性分析 28 4.3 人口學基本資料 31 4.4 重覆測量試驗 31 4.4.1 呼出氣體VOCs於抽菸者和非抽菸者的情形 31 4.4.2 抽菸影響呼出氣體VOCs的因子 36 4.4.3 呼出氣體VOCs於抽菸組和非抽菸組的差異 40 4.5 抽菸者長時間禁菸下和非抽菸者呼出氣體VOCs的差異 45 4.6 尿液中8-OHdG 52 4.7 氧化壓力指標和抽菸的相關性 56 4.7.1 呼氣中pentane 56 4.7.2 尿液中8-OHdG 56 第五章 討論 63 5.1 影響呼出氣體中VOCs的因子 63 5.2 內生性氣體和外生性氣體 64 5.3 長時間禁菸下抽菸者呼出氣體VOCs情形 65 5.4 尿液中8-OHdG 66 5.5 外生性氣體和氧化壓力指標的相關性 67 5.6 研究限制 69 5.7 結論 70 第六章 參考文獻 71 | |
| dc.language.iso | zh-TW | |
| dc.subject | 氣相層析儀 | zh_TW |
| dc.subject | 揮發性有機物 | zh_TW |
| dc.subject | 呼出氣體 | zh_TW |
| dc.subject | 抽菸 | zh_TW |
| dc.subject | 混合型模式 | zh_TW |
| dc.subject | Cigarette smoking | en |
| dc.subject | Mixed effect model | en |
| dc.subject | GC/MS | en |
| dc.subject | Volatile organic compounds(VOCs) | en |
| dc.subject | Exhaled breath | en |
| dc.title | 抽菸對人體呼出氣體中揮發性有機物質的影響 | zh_TW |
| dc.title | Effect of Smoking on Exhaled Breath Volatile Organic Compounds | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳保中(Pau-Chung Chen),蔡詩偉 | |
| dc.subject.keyword | 抽菸,呼出氣體,揮發性有機物,氣相層析儀,混合型模式, | zh_TW |
| dc.subject.keyword | Cigarette smoking,Exhaled breath,Volatile organic compounds(VOCs),GC/MS,Mixed effect model, | en |
| dc.relation.page | 81 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-24 | |
| dc.contributor.author-college | 公共衛生學院 | zh_TW |
| dc.contributor.author-dept | 環境衛生研究所 | zh_TW |
| 顯示於系所單位： | 環境衛生研究所 | |
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