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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29208完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李芳仁(FANG-JEN, LEE) | |
| dc.contributor.author | Kuan-Yu Chen | en |
| dc.contributor.author | 陳冠毓 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:02:49Z | - |
| dc.date.available | 2016-10-05 | |
| dc.date.copyright | 2011-10-05 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-04 | |
| dc.identifier.citation | Reference
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29208 | - |
| dc.description.abstract | 第一腺嘌呤核苷二磷酸核醣化因子相似蛋白(ARL1)是腺嘌呤核苷二磷酸核醣化因子(ADP-ribosylation factor, ARF)家族的一員。它們皆為小型鳥糞嘌呤核苷結合蛋白。ARF家族成員負責著細胞內的囊泡運輸、磷脂酶的活化、參與磷脂三激酶的訊息調控等等的功能。目前雖然已知有許多ARL的存在但對其功能卻仍幾乎是一無所知。近來，我們實驗室已經發現了Syt1p是Arl1p蛋白的鳥糞嘌呤核苷三磷酸(GTP)交換蛋白(GEF)。他會促使Arl1p與鳥糞嘌呤核苷三磷酸結合，並吸引其下游作用蛋白 Imh1p至高基氏體上，最後經由其活化蛋白(GAP)Gcs1p水解鳥糞嘌呤核苷三磷酸。Imh1p是高基氏體蛋白(golgin)的一種。而雖然在動物細胞中，高基氏體蛋白已被報導與調控、維持高基氏體的功能有關，但在酵母菌中，Imh1p的功能仍然不清楚。在本篇論文裡，我發現Imh1p在調控Arl1p的去活化中扮演一定的腳色。在酵母菌中去除IMH1基因會造成Arl1p失去與鳥糞嘌呤核苷三磷酸的結合，而造成Arl1p失去其正常應位於高基氏體的位置。在生體外，Imh1p與Gcs1p會互相競爭與Arl1p結合，Arl1p與Imh1p的結合會減少Gcs1p去活化Arl1p-GTP的能力。此外，Imh1p二聚體(dimer)的能力也對於Imh1p穩定Arl1p的功能是很重要的。因此，在本篇論文證明了Imh1p參與了Gcs1p於時間、空間上所調控Arl1p的鳥糞嘌呤核苷三磷酸循環。 | zh_TW |
| dc.description.abstract | ARF-like proteins (ARLs) are one of the ADP-ribosylation factors (ARFs) family that are small GTP-binding proteins. Arf proteins enhance the ADP-ribosyltransferase activity of c0060holera toxin and have an important role in vesicular transport. Several ARLs have been cloned from different organisms. However, the biological functions of ARLs and its interacting protiens remain largely unknown. Recently, we have shown that Syt1p function as a GEF that promotes activation of Arl1p and recruitment of a golgin protein, Imh1p, to the Golgi. Gcs1p hydrolyzes GTP of Arl1p to inactivate Arl1p at later stage. Although mammalian golgins are proposed to regulate structure and function of the TGN, the physiological function of Imh1p remains not clear. Here, we report that Imh1p has a role on modulating inactivation of Arl1p on the Golgi. Deletion of IMH1 decreases the level of GTP-bound form Arl1p resulting in less amounts of Arl1p resided on TGN. Imh1p competes with Gcs1p in interacting with Arl1p and decreases Gcs1p GAP activity on Arl1p in vitro. Furthermore, the dimerization of Imh1p is necessary for its function on Arl1p. Our findings demonstrate that Imh1p participates in regulating Gcs1p-depndent spatial and temporal control of the GTP hydrolysis cycle of Arl1p. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:02:49Z (GMT). No. of bitstreams: 1 ntu-100-D94448007-1.pdf: 14595055 bytes, checksum: 493c0531c3f347560820e2bc8f9e2d2c (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 謝誌 ---------------------------------------------------------------------------------------------- I
Table of Contents-------------------------------------------------------------------------------- II Abstract ----------------------------------------------------------------------------------------- IV 中文摘要 --------------------------------------------------------------------------------------- V Abbreviations -------------------------------------------------------------------------------- VI Introduction ---------------------------------------------------------------------------------- 1 Materials and Methods --------------------------------------------------------------------- 18 Results 1. Imh1p is needed for the Golgi localization of Arl1p -------------------------------- 34 2. Dimerization of Imh1p attenuates GTP hydrolysis of Arl1p --------------------- 36 3. Imh1p is involved in regulating the GTP hydrolysis of Arl1p. --------------------- 40 4. Imh1p-dependent Golgi localization of Arl1p specifically requires Gcs1p ----- 41 5. Imh1p competes with Gcs1p to interact with Arl1p in vitro ---------------------- 43 6. Imh1-C177 alerts the GAP activity of Gcs1p to Arl1p. ------------------------- 44 7. Both Imh1-C177 and mCherry-Imh1 fail to suppress temperature sensitivity in ypt6 cell. ---------------------------------------------------------------------------------- 45 Discussion ------------------------------------------------------------------------------------ 46 Tables Table 1. Yeast strains used in this study ------------------------------------------------ 54 Table 2. Primers used in this study ------------------------------------------------------- 55 Figures Figure 1. Regulation of ARF activity by GEFs that accelerate GTP binding and GAPs that activate GTP hydrolysis by ARF---------------------- 57 Figure 2. Structures of the Arf family G protein Arf1 in GDP- and GTP-bound states---------------------------------------------------------- 58 Figure 3. Schematic view of ArfGAP1/Gcs1p activation by ALPS motif recognizes curved membranes-------------------------------------------- 59 Figure 4. Model of golgin GMAP-210--------------------------------------------- 60 Figure 5. Model for the role of Arf1-GTP in the generation of transport vesicles---------------------------------------------------------------------- 61 Figure 6. Schematic model of recruitment of Golgin-245 to the Golgi membrane by Arl1--------------------------------------------------------- 63 Figure 7. A model for the Arl3p-Syt1p-Arl1p-Imh1p pathway----------------- 64 Figure 8. Imh1p is involved in the localization of Arl1p and Imh1p to the Golgi------------------------------------------------------------------------ 65 Figure 9. Imh1p affected the Golgi localization of endogenous Arl1-HA---- 66 Figure 10. Imh1p specifically affects the localization of Arl1p------------------- 67 Figure 11. Imh1p requires GRIP domain for the localization of Arl1p to the Golgi------------------------------------------------------------------------ 68 Figure 12. Dimerization force in coiled-coil region is required for stabilizin TGN---------------------------------------------------------------- 69 Figure 13. Structure prediction of Imh1p C terminus------------------------------ 73 Figure 14. mCherry-Imh1-C177N779S,L782S fails to stabilize Arl1p at TGN----- 71 Figure 15. Self-interaction and localization of mCherry/DsRed-tagged Imh1p 72 Figure 16. DsRed-Imh10C89, but not mCherry-Imh1-C89, stabilize Arl1p at Golgi------------------------------------------------------------------------ 73 Figure 17. DsRed-Imh1-C177, but not mCherry-Imh1-C177, remains localizing to the Golgi in Gcs1p-everexpressing cell----------------- 74 Figure 18. Activated Arl1p decreased in imh1 cell------------------------------ 75 Figure 19. Knockout GCS1 suppressed the dissociation of Arl1p from TGN in imh1 cells ------------------------------------------------------------- 76 Figure 20. Gcs1p is specifically required for the dissociation of Arl1p from TGN in imh1 cells-------------------------------------------------------- 77 Figure 21. Arl1-QL interact with Gcs1p and Imh1-C177 in vitro---------------- 78 Figure 22. Imh1-C177, but not Imh1-C177 GRIP, competes with Gcs1 to interact with Arl1----------------------------------------------------------- 79 Figure 23. Imh1-C177 alerts the GAP activity of Gcs1p to Arl1p--------------- 80 Figure 24. Imh1-FL (full length), but not Imh1-C177 or mCherry-Imh1-FL, suppresses temperature sensitivity in ypt6 cell---------------------- 82 Figure 25. A model for the Imh1p involved in stabilizing Arl1-GTP------------ 83 Reference ------------------------------------------------------------------------------------ 84 Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p -------------- 90 | |
| dc.language.iso | en | |
| dc.title | 酵母菌第一腺嘌呤核苷二磷酸核醣化因子相似蛋白與其結合蛋白的功能性探討 | zh_TW |
| dc.title | Functional Characterization of ADP-Ribosylation Factor-Like Protein 1 and its Interacting Proteins in Saccharomyces cerevisiae | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 陳瑞華,張智芬,施修明,鄧述諄,王昭雯 | |
| dc.subject.keyword | 第一腺嘌呤核苷,二磷酸核醣化因子相似蛋白,腺嘌呤核苷,二磷酸核醣化因子,高基氏體蛋白, | zh_TW |
| dc.subject.keyword | ARL1,ARF,golgin,Imh1, | en |
| dc.relation.page | 89 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-04 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| 顯示於系所單位： | 分子醫學研究所 | |
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