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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳益群(Yi-Chun Wu) | |
| dc.contributor.author | I-Ju Lee | en |
| dc.contributor.author | 李以如 | zh_TW |
| dc.date.accessioned | 2021-06-13T00:37:51Z | - |
| dc.date.available | 2008-07-26 | |
| dc.date.copyright | 2007-07-26 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-24 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29064 | - |
| dc.description.abstract | In Eukaryotes, the cell cycle is under delicate control. Different phosphorylation state of a protein often leads to different activity, thus a number of protein kinases play important roles in mitosis. The C. elegans tousled-like kinase TLK-1 is a serine/threonine kinase that is important for DNA condensation and segregation. The tlk-1(RNAi) embryos arrested at approximately 55 cells stage and showed aneuploidy. To study the expression pattern of TLK-1, we generated TLK-1 antibodies. We show that TLK-1 is localized in the interphase nucleus but diffuses to the cytosol after nuclear envelope breakdown during mitosis. We found that TLK-1 is likely to act in chromosome segregation through DLC-1. DLC-1, a subunit of cytoplasmic dynein complex, interacts with TLK-1 in a yeast 2-hybrid system. By using time-course microscopic analysis we show that the depletion of dlc-1 causes defects in spindle assembly and chromosome segregation. Using immuno-staining we found that DLC-1 is enriched in mitotic centrosomes and spindle in wild-type but not tlk-1(RNAi) embryos. We conclude TLK-1 may mediate chromosome segregation by controlling the localization of DLC-1 to the mitotic centrosomes and spindles. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T00:37:51Z (GMT). No. of bitstreams: 1 ntu-96-R94b43007-1.pdf: 3471946 bytes, checksum: d7ea24c3e3c8ff090009bb957c9aa257 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | Abstract...1
摘要...2 Introduction...3 Materials and Methods...8 Results...15 tlk-1(RNAi) embryos exhibit defects in DNA condensation and segregation...15 The depletion of tlk-1 affects kinetochore alignment...16 TLK-1 diffuses to cytosol when a cell processes from prophase to metaphase...17 The localization of TLK-1 is not regulated through APC-targeted ubiquitination...18 dlc-1(RNAi) causes embryonic lethality...19 dlc-1(RNAi) does not affect the localization of TLK-1...20 dlc-1(RNAi) embryos exhibit defects in mitotic spindle assembly and chromosome segregation...20 DLC-1 gathers around centrosomes during mitosis...22 The localization of DLC-1 is impaired in tlk-1(RNAi)...22 Discussion...24 The condensation defect in tlk-1(RNAi...24 TLK-1 and the holocentric proteins...25 The segregation defect in tlk-1(RNAi)...25 The regulation of TLK-1 during cell cycle...26 The embryonic lethality of dlc-1(RNAi) mutants in different strains...27 The interaction between TLK-1 and DLC-1...27 The functions of DLC-1 and dynein complex in mitosis...29 Future perspectives...31 Figures and Tables...32 Reference...50 Appendix...54 | |
| dc.language.iso | en | |
| dc.subject | 細胞分裂 | zh_TW |
| dc.subject | 線蟲 | zh_TW |
| dc.subject | 有絲分裂 | zh_TW |
| dc.subject | C. elegans | en |
| dc.subject | mitosis | en |
| dc.title | 分析蛋白質TLK-1和DLC-1在線蟲有絲分裂中的特性 | zh_TW |
| dc.title | Characterization of TLK-1 and DLC-1 in C. elegans mitosis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖國楨(Gwo-Jen Liaw),黃偉邦(Wei-Pang Huang) | |
| dc.subject.keyword | 線蟲,有絲分裂,細胞分裂, | zh_TW |
| dc.subject.keyword | C. elegans,mitosis, | en |
| dc.relation.page | 53 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-25 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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