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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 施秀惠(Hsiu-Hui Shih) | |
| dc.contributor.author | Chien-Jen Tseng | en |
| dc.contributor.author | 曾建仁 | zh_TW |
| dc.date.accessioned | 2021-06-13T00:25:14Z | - |
| dc.date.available | 2008-07-30 | |
| dc.date.copyright | 2007-07-30 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-27 | |
| dc.identifier.citation | 林俊嘉 (2006)，安尼線蟲科原蟲寄生於真鰺影響因素之探討。碩士論文，國立台灣大學動物學研究所。
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Pellerone FI, Archer SK, Behm CA, Grant WN, Lacey MJ, Somerville AC. (2003) Trehalose metabolism genes in Caenorhabditis elegans and filarial nematodes. International journal for parasitology. 233:1195-1206. Petersen A, Schneider H, Rau G, Glasmacher B. (2006) A new approach for freezing of aqueous solutions under active control of the nucleation temperature. Cryobiology. 53:248-257. Ramon M, Rolland F. (2007) Plant development: introducing trehalose metabolism. Trends in plant science. 12:185-188. Rodriguez-Mahillo AI, Gonzalez-Munoz M, Gomez-Aguado F, Rodriguez-Perez R, Corcuera MT, Caballero ML, Moneo I. (2007) Cloning and characterisation of the Anisakis simplex allergen Ani s 4 as a cysteine-protease inhibitor. International journal for parasitology. 37:907-917. Sánchez-Monsálvez I, de Armas-Serra C, Bernadina W, Rodríguez-Caabeiro F. 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(2007) Acclimation effects on thermal tolerances of springtails from sub-Antarctic Marion Island: indigenous and invasive species. Journal of insect physiology. 53:113-125. Smith JW, Wootten R (1978) Anisakis and anisakiasis. Advances in parasitology. 16:98-163. Southon TE, Gehrken U. (1997) Effect of temperature on cold-hardiness and tissue ice formation in the adult chrysomelid beetle Melasoma collaris L. Journal of insect physiology. 43:587-593. Stromnes E, Andersen K. (2000) 'Spring rise' of whaleworm (Anisakis simplex; Nematoda, Ascaridoidea) third-stage larvae in some fish species from Norwegian waters. Parasitology research. 86:619-624. Styrvold OB, Strom AR. (1991) Synthesis, accumulation, and excretion of trehalose in osmotically stressed Escherichia coli K-12 strains: influence of amber suppressors and function of the periplasmic trehalase. Journal of bacteriology. 173:1187-1192. Umehara A, Kawakami Y, Matsui T, Araki J, Uchida A. 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(2003) Ice nucleation in nature: supercooling point (SCP) measurements and the role of heterogeneous nucleation. Cryobiology. 46:88-98. Woodruff PJ, Carlson BL, Siridechadilok B, Pratt MR, Senaratne RH, Mougous JD, Riley LW, Williams SJ, Bertozzi CR. (2004) Trehalose is required for growth of Mycobacterium smegmatis. The Journal of biological chemistry. 279:28835-28843. Zachariassen KE. (1985) Physiology of cold tolerance in insects. Physiological reviews. 65:799-832. Zachariassen KE, Kristiansen E. (2000) Ice nucleation and antinucleation in nature. Cryobiology. 41:257-279. Zhu X., Amelio S.D., Paggi L. and Gasser R.B. (2000) Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitology Research. 86:677-683. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28838 | - |
| dc.description.abstract | 海獸胃線蟲是海洋經濟魚類常見之蠕蟲類寄生蟲，同時是引起人類海獸胃線蟲症之病原體。由於海獸胃線蟲對冷凍具有抗性，因此食品安全上建議在食用漁產品前需要冷凍足夠時間和溫度以避免被感染。本研究旨在查明海獸胃線蟲經過低溫處理後所產生之抗凍機制，主要探討抗凍蛋白存在與海藻糖生成酶在低溫處理下之活性。使用採集自蘇澳外海鯖魚體內之海獸胃線蟲，以基因體方法和冰親和性純化以了解是否以抗凍蛋白為抗凍機制，結果證明海獸胃線蟲體內並無抗凍蛋白存在。在海藻糖生成酶活性方面，將海獸胃線蟲低溫處理0、4和10 ℃後，所得結果海藻糖生成酶比活性為0.235、0.632和0.819 Unit/mg，對照組16 ℃則為0.151 Unit/mg，在統計上4 ℃及10 ℃和對照組有顯著差異，溫度降低確實使酵素活性增加。0 ℃時和對照組無顯著差異，則推測降溫太快使海獸胃線蟲無法引起抗凍反應表現海藻糖生成酶。而在4 ℃低溫刺激下，12小時海藻糖生成酶活性即有顯著上升，到30小時活性仍有增加。綜和以上的實驗結果，推論海藻糖在海獸胃線蟲中扮演重要的抗凍保護劑，且需要經過低溫刺激才能產生，也支持海獸胃線蟲具有忍耐抗凍能力。 | zh_TW |
| dc.description.abstract | Anisakis simplex is a famous helminth parasite of commercial marine fish, which is the pathogen of Anisakiasis. Because A. simplex can survive in freezing temperature, for food safety, it is suggested to keep fishes under freezing temperature long enough to avoid infection before eating sea foods. In this study, the purposes are to investigate antifreeze mechanism of cold treatment, mainly discusses the antifreeze protein existence and the trehalose synthase activity under the low-temperature treatment. This study used the gathering of A. simplex from Spotted Mackerel that were obtained from commercial local fisheries in coastal waters of Su-Ao. The results showed A. simplex does not have the antifreeze protein existence by the genomic analysis and the ice affinity purification. In the aspect of trehalose synthase activity, the activity of trehalose synthase of A. simplex under the low-temperature treatment of 0, 4 and 10 ℃ was 0.235, 0.632 and 0.819 Unit/mg, respectively, and the activity of control group ( treatment of 16 ℃) was 0.151 Unit/mg. In statistics, the result of 4 and 10 ℃ treatment showed remarkable differences with the control group, that is, the decreasing ot temperature indeed caused the increasing of enzyme activity. There is no significant difference between 0 ℃ treatment and control group. It is deduces that, quick temperature decreasing can not induce the anti-freeze response of A. simplex, thus no expression of trehalose synthase. Under 4 ℃ low temperature treatment, the activity of trehalose synthase increased significantly after 12 hours, and remained increased afer 30 hours. In summary, the trehalose may acts as an important cryoprotectant in the A. simplex, but the low temperature stimulation is needed for the production of trehalose. This study supports that A. simplex has the ability of freeze-tolerant. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T00:25:14Z (GMT). No. of bitstreams: 1 ntu-96-R93b41015-1.pdf: 975145 bytes, checksum: 5c55f05602e0020cb28da2fbd58c5e63 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
誌謝 II 中文摘要 III 英文摘要 IV 壹、前言 一、海獸胃線蟲 01 1. 海獸胃線蟲之概述 01 2. 海獸胃線蟲之生活史 02 3. 海獸胃線蟲之特徵與鑑定 04 4. 海獸胃線蟲症 06 5. 海獸胃線蟲之食品安全與抗凍能力 07 二、生物抗凍 08 1. 生物抗凍之名詞定義 08 2. 生物抗凍之策略 10 3. 冷凍保護劑之簡介 13 三、海藻糖 15 1. 海藻糖之簡介 15 2. 海藻糖之結構 15 3. 海藻糖之合成 16 4. 海藻糖之功用 18 四、研究動機與目的 19 貳、材料與方法 一、實驗藥品與試劑 20 二、實驗儀器設備 21 三、研究方法及步驟 21 1.樣品取得 21 2.樣品處理 22 3.海獸胃線蟲形態鑑定 22 4.海獸胃線蟲體外培養 22 5.Genomic DNA萃取 23 6.聚合酶連鎖反應 24 7.DNA電泳和ethidium bromide（EtBr）染色 25 8.幼蟲低溫刺激處理 25 9.總量蛋白質粗萃取 25 10.Bradford protein assay 蛋白質定量 26 11.冰親和性純化 (Ice affinity purification) 27 12.蛋白質濃縮方法 28 13.硫酸十二酯鈉聚丙烯醯氯膠電泳（SDS-PAGE） 28 14.Coomassie blue R-250 蛋白質染色 29 15.海藻糖酵素活性測試 30 16.統計方法 32 參、結果 一、海獸胃線蟲抗凍蛋白基因體分析 33 二、海獸胃線蟲抗凍蛋白之冰親和性分析 34 三、不同低溫刺激對海藻糖生成酶活性之效應 36 四、海獸胃線蟲經4 ℃處理後其海藻糖生成酶之活性變化 37 肆、討論 一、 選殖海獸胃線蟲抗凍蛋白基因策略之分析探討 38 二、 利用冰親和性策略純化海獸胃線蟲抗凍蛋白之探討 41 三、海藻糖生成酶在低溫處理下之探討 42 四、採樣遭遇困難之探討 45 五、未來研究目標 46 伍、參考文獻 47 | |
| dc.language.iso | zh-TW | |
| dc.subject | 海獸胃線蟲症 | zh_TW |
| dc.subject | 海藻糖生成酶 | zh_TW |
| dc.subject | 抗凍 | zh_TW |
| dc.subject | 海獸胃線蟲 | zh_TW |
| dc.subject | Antifreeze | en |
| dc.subject | Anisakis simplex | en |
| dc.subject | Anisakiasis | en |
| dc.subject | Trehalose synthase | en |
| dc.title | 海獸胃線蟲之海藻糖生成酶活性與低溫相關性研究 | zh_TW |
| dc.title | Study of the Corelations between
Low Temperature and the Trehalose Synthase Activity of Anisakis simplex | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王蓮成,陳哲俊 | |
| dc.subject.keyword | 海獸胃線蟲,海獸胃線蟲症,海藻糖生成酶,抗凍, | zh_TW |
| dc.subject.keyword | Anisakis simplex,Anisakiasis,Trehalose synthase,Antifreeze, | en |
| dc.relation.page | 65 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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