請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28718
標題: | 三價鉻離子增強Luminol-KIO4之化學發光性質研究與應用 Enhancement of Chemiluminescence of the Luminol-KIO4 System by Cr(III) and Its Applications |
作者: | Chiou-Ling Huang 黃秋鈴 |
指導教授: | 林萬寅 |
關鍵字: | 流動注入分析法,三價鉻離子,活性含氧物質,苯二酚,兒茶酚胺,苯三酚,色胺酸, flow injection analysis,chromium(III) ion,reactive oxygen species,benzenediol,catecholamines,benzenetriol,tryptophan, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | We have studied the enhancement in CL for luminol-KIO4 system caused by chromium(III) ion using flow injection analysis. For the KIO4/ luminol CL systm﹐about 100-fold increase in CL intensity was observed upon addition of 150 μM Cr(III) at pH 13. The effect of concentration of reagents (chromium(III)﹐luminol﹐potassium periodate)﹐pH﹐flow rate﹐and modes of reagent mixing on CL emission were also investigated and optimized.
In emission spectrum, the CL maximum occurred at 429 nm. So the CL is caused by luminol in Cr(III)/ KIO4/ luminol system. When the dissolved oxygen was removed from the solution by purging with nitrogen﹐the CL decreased by 20 %. This might indicate that superoxide ion played a minor role in this CL reaction. In order to know the mechanism of the CL of Cr(III)/ KIO4/ luminol system, we tested a series of free radical scanvengers for their effect on CL emission. The CL intensity decreased greatly in the presence of these scavengers. These results showed that in addition to •O2-, 1O2 and •OH also participated in CL reaction. The CL-enhancement may result from the reaction of KIO4 and dissolved oxygen in strong alkaline solution to produce reactive oxygen species (ROS) in the prescence of catalytic Cr3+. However, the major reason for CL-enhancement would be the formation of LuH-Cr-IO4 complex that increases the oxidation of luminol. By using the CL system, we tested 44 compounds for their effect on the CL emission, including amino acids, polyphenol, catecholamine, nitrogenous bases, vitamins, etc. The results showed that the system has high selectivity for tryptophan. Furthermore, we found that common antioxidants such as benzenediols and catecholamines showed inhibiting effect on our CL system because these compounds might eliminate •O2-. On the contrary, the CL emission increased at low concentration of benzenetriol because of the generation of more •O2- upon oxidation of benzenetriol .Nevertheless, the CL emission decreased at high concentration. Finally we did a series of optimization for the best inhibitory effect of hydroquinone and catechol, to achieve low detection limits of 1.02 nM and 0.96 nM respectively. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28718 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 1.4 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。