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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李水盛 | |
| dc.contributor.author | Yi-Shan Lin | en |
| dc.contributor.author | 林憶珊 | zh_TW |
| dc.date.accessioned | 2021-06-13T00:11:00Z | - |
| dc.date.available | 2009-08-08 | |
| dc.date.copyright | 2007-08-08 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-27 | |
| dc.identifier.citation | 1. Flora of Taiwan, 2nd ed.477-484, Editorial Committee of the Flora of Taiwan, Taipei, Taiwan. (1996)
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Melanin biosynthesis inhibitors from the bark of Machilus thunbergii. Biological & Pharmaceutical Bulletin 26, 1039-1041 (2003). 9. Ma, C.J., Sung, S.H. & Kim, Y.C. Neuroprotective lignans from the bark of Machilus thunbergii. Planta Medica 70, 79-80 (2004). 10. Li, G. et al. Lignans from the bark of Machilus thunbergii and their DNA topoisomerases I and II inhibition and cytotoxicity. Biological & Pharmaceutical Bulletin 27, 1147-1150 (2004). 11. Park, B.Y. et al. Increase of caspase-3 activity by lignans from Machilus thunbergii in HL-60 cells. Biological & Pharmaceutical Bulletin 27, 1305-1307 (2004). 12. Komae, H. & Hayashi, N. Terpenes from Actinodaphne, Machilus and Neolitsea Species. Phytochemistry 11, 1181-& (1972). 13. Komae, H. & Hayashi, H. Phytosterols of Trunks of Lindera-Obtusiloba. Phytochemistry 11, 1182-& (1972). 14. Lu, S.T. Studies on the Alkaloids of Formosan Louraceous Plants.Ⅱ. Alkaloids of Machilus kusanoi Hayata. (2). The Isolation of dl-Coclaurine. 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Tsai, I.L., Chen, J.H., Duh, C.Y. & Chen, I.S. Cytotoxic neolignans and butanolides from Machilus obovatifolia. Planta Medica 67, 559-561 (2001). 21. Takaoka, D., Watanabe, K. & Hiroi, M. Studies on Lignoids in Lauraceae .2. Studies on Lignans in Leaves of Machilus-Japonica Sieb Et Zucc. Bulletin of the Chemical Society of Japan 49, 3564-3566 (1976). 22. Gonzalezcoloma, A., Escoubas, P., Mizutani, J. & Lajide, L. Insect Growth-Inhibitors from Machilus-Japonica. Phytochemistry 35, 607-610 (1994). 23. Komae, H. & Hayashi, N. Terpenic Constituents from Machilus-Japonica. Phytochemistry 10, 3311-& (1971). 24. Martin, A.E. & Montgomery, P.A. Acarbose: An alpha-glucosidase inhibitor. American Journal of Health-System Pharmacy 53, 2277-2290 (1996). 25. Puls, W., Keup, U., Krause, H.P., Thomas, G. & Hoffmeister, F. Glucosidase Inhibition - New Approach to Treatment of Diabetes, Obesity, and Hyperlipoproteinemia. Naturwissenschaften 64, 536-537 (1977). 26. Bischoff, H. Pharmacology of Alpha-Glucosidase Inhibition. European Journal of Clinical Investigation 24, 3-10 (1994). 27. Grotewold, E. The Science of Flavonoids, 1st ed. 1-4 , Springer, USA. (2006). 28. Heijnen, C.G.M., Haenen, G., van Acker, F.A.A., van der Vijgh, W.J.F. & Bast, A. Flavonoids as peroxynitrite scavengers: the role of the hydroxyl groups. Toxicology in Vitro 15, 3-6 (2001). 29. Chun, O.K., Kim, D.O. & Lee, C.Y. Superoxide radical scavenging activity of the major polyphenols in fresh plums. Journal of Agricultural and Food Chemistry 51, 8067-8072 (2003). 30. Mira, L. et al. Interactions of flavonoids with iron and copper ions: A mechanism for their antioxidant activity. Free Radical Research 36, 1199-1208 (2002). 31. Cheng, I.F. & Breen, K. On the ability of four flavonoids, baicilein, luteolin, naringenin, and quercetin, to suppress the fenton reaction of the iron-ATP complex. Biometals 13, 77-83 (2000). 32. Williams, R.J., Spencer, J.P.E. & Rice-Evans, C. Flavonoids: Antioxidants or signalling molecules? Free Radical Biology and Medicine 36, 838-849 (2004). 33. Spencer, J.P.E., Rice-Evans, C. & Williams, R.J. Modulation of pro-survival Akt/protein kinase B and ERK1/2 signaling cascades by quercetin and its in vivo metabolites underlie their action on neuronal viability. Journal of Biological Chemistry 278, 34783-34793 (2003). 34. Spencer, J.P.E. et al. Contrasting influences of glucuronidation and O-methylation of epicatechin on hydrogen peroxide-induced cell death in neurons and fibroblasts. Free Radical Biology and Medicine 31, 1139-1146 (2001). 35. Hou, Z., Lambert, J.D., Chin, K.V. & Yang, C.S. Effects of tea polyphenols on signal transduction pathways related to cancer chemoprevention. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 555, 3-19 (2004). 36. Lambert, J.D. & Yang, C.S. Mechanisms of cancer prevention by tea constituents. Journal of Nutrition 133, 3262S-3267S (2003). 37. Wang, P.H. & Lee, S.S. Polar chemical constituents from Phoebe formosana. Journal of the Chinese Chemical Society 46, 215-219 (1999). 38. Oyama, K. & Kondo, T. Total synthesis of apigenin 7,4 '-di-O-beta-glucopyranoside, a component of blue flower pigment of Salvia patens, and seven chiral analogues. Tetrahedron 60, 2025-2034 (2004). 39. Lee, S.S., Chang, S.M. & Chen, C.H. Chemical constituents from Alseodaphne andersonii. Journal of Natural Products 64, 1548-1551 (2001). 40. Lee, S.S., Wang, J.S. & Chen, K.C.S. Chemical-Constituents from the Roots of Zizyphus-Jujuba Mill Var Spinosa .1. Journal of the Chinese Chemical Society 42, 77-82 (1995). 41. Salvador, M.J. et al. Isolation and HPLC quantitative analysis of antioxidant flavonoids from Alternanthera tenella Colla. Zeitschrift Fur Naturforschung C-a Journal of Biosciences 61, 19-25 (2006). 42. Kim, J.H. et al. The isolation and antioxidative effects of vitexin from Acer palmatum. Archives of Pharmacal Research 28, 195-202 (2005). 43. Lin, C.M. et al. Isovitexin suppresses lipopolysaccharide-mediated inducible nitric oxide synthase through inhibition of NF-kappa B in mouse macrophages. Planta Medica 71, 748-753 (2005). 44. Wang, Y.M. et al. Depletion of intracellular glutathione mediates butenolide-induced cytotoxicity in HepG2 cells. Toxicology Letters 164, 231-238 (2006). | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28528 | - |
| dc.description.abstract | 在本研究中，一開始依據活性測試結果為導向之分離 (bioassay-guided separation)，尋找對甲型葡萄糖水解酶(α-glucosidase)有抑制作用的化合物。選定樟科楨楠屬植物大葉楠Machilus japonica Sieb. ＆ Zucc. var. kusanoi (Hayata) Liao (Lauraceae)葉部為研究目標，首先將酒精萃取物，經極性劃分為二氯甲烷、乙酸乙酯、正丁醇和水可溶四部分，並針對正丁醇可溶部分進行研究。但發現活性部分不強且成分散置，因此以一般方法進行正丁醇可溶部分之成分研究，經分離得到八個屬於類黃酮成分的化合物：2'-O-α-L-Rhamnopyranosyl isovitexin (1)，2'-O-α-L-Rhamnopyranosyl vitexin (2)，Apigenin 4'-O-β-D-glucopyranoside (3)，化合物4，化合物5，化合物6，Quercetin-3-O-β-D-galactoside (7)和Quercetin-3-O-β-D-glucoside (8)。其中化合物1到6屬於黃酮 (flavones)，而化合物7和8則屬於黃酮醇 (flavonols)，化合物4、5、6則為新架構天然物。
這一系列化合物對α-glucosidase的抑制效果並不理想，前六個化合物中，僅化合物3、5、6在100 μg/mL呈現強弱不一的抑制活性，抑制百分比依序為64.7%、18.7%及93.7%，然而在10 μg/mL濃度時皆無活性。 | zh_TW |
| dc.description.abstract | Guided by bioassay against α-glucosidase, the active constituents of the leaves extract of Machilus japonica Sieb. ＆ Zucc. var. kusanoi (Hayata) Liao (Lauraceae) were investigated in this study. Several attempts were made to concentrate the active fractions from this approach. However, it was found that the activity was not strong in each fraction. Then, the aim of this study turned to the separation of chemical constituents of the polar fractions via various chromatographic techniques. The effects led to the identification of eight compounds, 2'-O-α-L-rhamnopyranosyl isovitexin (1), 2'-O-α-L-rhamnopyranosyl vitexin (2), apigenin 4'-O-β-D-glucopyranoside (3), compound (4), compound (5), compound (6), quercetin-3-O-β-D-galactoside (7) and quercetin-3-O-β-D-glucoside (8). Compounds 1-6 belong to flavones and compounds 7-8 belong to flavonols. In addition, compounds 4-6 are disclosed to be new natural products, possessing a novel skeleton.
These compounds exhibit weak inhibitory activity against α-glucosidase. Of these compounds tested (1-6), compounds 3, 5 and 6 exhibit 64.7 %, 18.7 % and 93.7 % of inhibitory activity, respectively, at a concentration level of 100 μg/mL; but no activity was obsevered for each compound at 10 μg/mL level. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T00:11:00Z (GMT). No. of bitstreams: 1 ntu-96-R94423026-1.pdf: 7261796 bytes, checksum: fe6c06a9dd522f693c769d5cc1628439 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 總目錄
口試委員會審定書 i 誌謝 ii 中文摘要 iii Abstract iv 目錄 I 流程圖目錄 Ⅱ 表目錄 Ⅱ 圖目錄 Ⅲ 參考文獻 86 附圖 93 目錄 壹、序論 1 1-1研究目的 1 1-2植物簡介 3 1-3 Machilus屬植物成分之文獻回顧 4 1-4 α-Glucosidase inhibitors簡介 17 1-5 類黃酮成分簡介 19 1-5-1 命名 19 1-5-2 生物活性 21 貳、實驗儀器與材料 22 2-1理化性質測定儀器 22 2-2成分分離之儀器及材料 22 2-3 試劑與溶媒 23 2-4 甲型葡萄糖水解酶之活性試驗 (α-Glucosidase assay) 所用試劑與儀器 24 參、實驗結果與討論 25 3-1 活性導向分離條件探索 25 3-2 類黃酮成分之分離及結構解析 30 3-2-1 2'-O-α-L-Rhamnopyranosyl isovitexin (1)之結構解析 32 3-2-2 2'-O-α-L-Rhamnopyranosyl vitexin (2)之結構解析 38 3-2-3 Apigenin 4'-O-β-D-glucopyranoside (3)之結構解析 42 3-2-4 化合物4之結構解析 45 3-2-5 化合物5之結構解析 51 3-2-6 化合物6之結構解析 55 3-2-7 化合物4 ~ 6之可能生合成途徑 60 3-2-8 Quercetin-3-O-β-D-galactoside (7)之結構解析 61 3-2-9 Quercetin-3-O-β-D-glucoside (8)之結構解析 64 3-3 化合物1-6活性測試結果 67 3-4 一系列同分異構物分離之困難點討論 68 3-5 結論 70 肆、實驗部分 71 4-1 甲型葡萄糖水解酶之活性試驗 (α-Glucosidase assay) 71 4-1-1 原理 71 4-1-2 酵素活性單位 71 4-1-3 實驗方法 71 4-2大葉楠葉部成分之萃取與分離 74 4-3 化合物分離 75 4-3-1 活性導向部分的分離 76 4-3-2 2'-O-α-L-Rhamnopyranosyl isovitexin (1)的分離和2'-O-α-L-Rhamnopyranosyl vitexin (2)的分離 78 4-3-3 Apigenin 4'-O-β-D-glucopyranoside (3)的分離 79 4-3-4 化合物4的分離 79 4-3-5 化合物5的分離 80 4-3-6 化合物6的分離 80 4-3-7 Quercetin-3-O-β-D-galactoside (7)和Quercetin-3-O-β-D-glucoside (8)的分離 81 4-3-8 化合物1-8之物理數據 82 流程圖目錄 Scheme 1. The possible biosynthetic pathway of compounds 4 ~ 6. 60 Scheme 2. The principle of α-glucosidase assay. 71 Scheme 3. Fractionation of EtOH extract of Machilus kusanoi Hayata. 74 Scheme 4. Separation process of compounds 1-8 from n-BuOH soluble fraction. 75 表目錄 Table 1. Chemical constituents from the Machilus plants (Lauraceae) 4 Table 2. α-glucosidase inhibitors in clinical use. 17 Table 3. 1H (400 MHz) and 13C NMR (100 MHz) data and HMBC correlation of compound 1 (CD3OD) 37 Table 4. 1H (400 MHz) and 13C NMR (100 MHz) data and HMBC correlation of compound 2 (CD3OD) 41 Table 5. 1H (400 MHz)and 13C NMR (50 MHz) of compound 3 (CD3OD) and the reported data for apigenin 4'-O-β-D-glucopyranoside and 43 Table 6. 1H (400 MHz) and 13C NMR (100 MHz) data and HMBC correlation of compound 4 (CD3OD) 49 Table 7. 1H (400 MHz) and 13C NMR (100 MHz) data and HMBC correlation of compound 5 (CD3OD) 53 Table 8. 1H (400 MHz) and 13C NMR (100 MHz) data and HMBC correlation of compound 6 (CD3OD) 58 Table 9. 1H (400 MHz) and 13C NMR (50 MHz) of compound 7 (CD3OD) and 62 the reported data for quercetin-3-O-β-D-galactoside (C5D5N, 400 MHz) 62 Table 10. 1H (400 MHz) and 13C NMR (100 MHz) of compound 8 (CD3OD) and the reported data for quercetin-3-O-β-D-glucoside (CD3OD, 400 MHz) 65 Table 11. Separation result of compounds 1 and 2 by Sephadex LH-20 [MeOH-H2O system]. 68 圖目錄 Figure 1. 大葉楠Machilus japonica Sieb. & Zucc. var. kusanoi (Hayata) Liao (Lauraceae) 2 Figure 2. Structure of α-glucosidase inhibitors in clinical use. 18 Figure 3. 1H-NMR assignment for compound 1 (CD3OD, 400 MHz) 34 Figure 4. 13C-NMR assignment for compound 1 (CD3OD, 100 MHz) 35 Figure 5. Key NOESY correlations of compound 1 (CD3OD, 400 MHz) 35 Figure 6. Key COSY correlations of compound 1 (CD3OD, 400 MHz) 36 Figure 7. Key HMBC correlations of compound 1 (CD3OD, 400 MHz) 36 Figure 8. 1H-NMR assignment for compound 2 (CD3OD, 400 MHz) 39 Figure 9. 13C-NMR assignment for compound 2 (CD3OD, 100 MHz) 39 Figure 10. Key COSY correlations of compound 2 (CD3OD, 400 MHz) 40 Figure 11. Key HMBC correlations of compound 2 (CD3OD, 400 MHz) 40 Figure 12. 1H-NMR assignment for compound 3 (CD3OD, 400 MHz) 44 Figure 13. 13C-NMR assignment for compound 3 (CD3OD, 50 MHz) 44 Figure 14. 1H-NMR assignment for compound 4 (CD3OD, 400 MHz) 47 Figure 15. 13C-NMR assignment for compound 4 (CD3OD, 100 MHz) 47 Figure 16. Key COSY correlations of compound 4 (CD3OD, 400 MHz) 48 Figure 17. Key NOESY correlations of compound 4 (CD3OD, 400 MHz) 48 Figure 18. Key HMBC correlations of compound 4 (CD3OD, 400 MHz) 50 Figure 19. 1H-NMR assignment for compound 5 (CD3OD, 400 MHz) 52 Figure 20. 13C-NMR assignment for compound 5 (CD3OD, 100 MHz) 52 Figure 21. Key HMBC correlations of compound 5 (CD3OD, 400 MHz) 54 Figure 22. 1H-NMR assignment for compound 6 (CD3OD, 400 MHz). 56 Figure 23. 13C-NMR assignment for compound 6 (CD3OD, 100 MHz) 56 Figure 24. Key COSY correlations of compound 6 (CD3OD, 400 MHz) 57 Figure 25. Key NOESY correlations of compound 6 (CD3OD, 400 MHz) 57 Figure 26. Key HMBC correlations of compound 6 (CD3OD, 400 MHz) 59 Figure 27. 1H-NMR assignment for compound 7 (CD3OD, 400 MHz) 63 Figure 28. 13C-NMR assignment for compound 7 (CD3OD, 50 MHz) 63 Figure 29. 1H-NMR assignment for compound 8 (CD3OD, 400 MHz) 66 Figure 30. 13C-NMR assignment for compound 8 (CD3OD, 100 MHz) 66 Figure 31. HPLC sepectrum of Fr. D-2-12 69 Figure 32. 96孔微量測試盤上各組測試樣品的排列情形 73 Figure 33. CD spectra of compounds 1, 2 and 3 (MeOH)…………………………..94 Figure 34. CD spectra of compounds 4, 5 and 6 (MeOH).…………………………..95 Figure 35. CD spectra of compounds 7 and 8 (MeOH).……………………………..96 Figure 36. UV spectra of compounds 1, 2 and 3 (MeOH). ………….……………... 97 Figure 37. UV spectra of compounds 4, 5 and 6 (MeOH). ………….……………....98 Figure 38. UV spectra of compounds 7 and 8 (MeOH). ………….………………....99 Figure 39. IR spectra of compounds 4, 5 and 6. …………….…….………………..100 Figure 40. MS and MS/MS spectra of compound 1 (MeOH). ..……………………101 Figure 41. MS and MS/MS spectra of compound 2 (MeOH)…………………...….102 Figure 42. MS and MS/MS spectra of compound 3 (MeOH)…………………...….103 Figure 43. MS and MS/MS spectra of compound 4 (MeOH)…………………...….104 Figure 44. MS and MS/MS spectra of compound 5 (MeOH)………………...….....105 Figure 45. MS and MS/MS spectra of compound 6 (MeOH)...………………...…..106 Figure 46. MS and MS/MS spectra of compound 7 (MeOH)…………………...….107 Figure 47. MS and MS/MS spectra of compound 8 (MeOH).. ..………………...…108 Figure 48. 1H-NMR spectrum of compound 1 (CD3OD, 400 MHz). …………...…109 Figure 49. 13C-NMR spectrum of compound 1 (CD3OD, 100 MHz). ……………..110 Figure 50. COSY spectrum of compound 1 (CD3OD, 400 MHz). ………….......….111 Figure 51. NOESY (1) spectrum of compound 1 (CD3OD, 400 MHz). ………..….112 Figure 52. NOESY (2) spectrum of compound 1 (CD3OD, 400 MHz). ………..….113 Figure 53. HMQC (1) spectrum of compound 1 (CD3OD, 400 MHz). …………....114 Figure 54. HMQC (2) spectrum of compound 1 (CD3OD, 400 MHz). ……..……..115 Figure 55. HMQC (3) spectrum of compound 1 (CD3OD, 400 MHz). ……..…......116 Figure 56. HMBC (1) spectrum of compound 1 (CD3OD, 400 MHz). ………….....117 Figure 57. HMBC (2) spectrum of compound 1 (CD3OD, 400 MHz). ……..……...118 Figure 58. HMBC (3) spectrum of compound 1 (CD3OD, 400 MHz). …………….119 Figure 59. 1H-NMR spectrum of compound 2 (CD3OD, 400 MHz). …………..….120 Figure 60. 13C-NMR spectrum of compound 2 (CD3OD, 100 MHz). ………..……121 Figure 61. COSY (1) spectrum of compound 2 (CD3OD, 400 MHz). ………….….122 Figure 62. COSY (2) spectrum of compound 2 (CD3OD, 400 MHz). ………….….123 Figure 63. COSY (3) spectrum of compound 2 (CD3OD, 400 MHz). ………..……124 Figure 64. HMQC (1) spectrum of compound 2 (CD3OD, 400 MHz). ………..…..125 Figure 65. HMQC (2) spectrum of compound 2 (CD3OD, 400 MHz). …………....126 Figure 66. HMQC (3) spectrum of compound 2 (CD3OD, 400 MHz). …………....127 Figure 67. HMBC (1) spectrum of compound 2 (CD3OD, 400 MHz). ………..…...128 Figure 68. HMBC (2) spectrum of compound 2 (CD3OD, 400 MHz). ………..…...129 Figure 69. HMBC (3) spectrum of compound 2 (CD3OD, 400 MHz). ………..…...130 Figure 70. 1H-NMR spectrum of compound 3 (CD3OD, 400 MHz). ………..…….131 Figure 71. 13C-NMR spectrum of compound 3 (CD3OD, 50 MHz). ………..…..…132 Figure 72. 1H-NMR spectrum of compound 4 (CD3OD, 400 MHz). ………..….....133 Figure 73. 13C-NMR spectrum of compound 4 (CD3OD, 100 MHz). ………..…....134 Figure 74. COSY (1) spectrum of compound 4 (CD3OD, 400 MHz). ………..…....135 Figure 75. COSY (2) spectrum of compound 4 (CD3OD, 400 MHz). ………..…....136 Figure 76. COSY (3) spectrum of compound 4 (CD3OD, 400 MHz). ………..…....137 Figure 77. NOESY (1) spectrum of compound 4 (CD3OD, 400 MHz). ………..….138 Figure 78. NOESY (2) spectrum of compound 4 (CD3OD, 400 MHz). ……….…..139 Figure 79. HMQC (1) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..140 Figure 80. HMQC (2) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..141 Figure 81. HMQC (3) spectrum of compound 4 (CD3OD, 400 MHz). ……………142 Figure 82. HMQC (4) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..143 Figure 83. HMBC (1) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..144 Figure 84. HMBC (2) spectrum of compound 4 (CD3OD, 400 MHz). ……………145 Figure 85. HMBC (3) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..146 Figure 86. HMBC (4) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..147 Figure 87. HMBC (5) spectrum of compound 4 (CD3OD, 400 MHz). ………..…..148 Figure 88. 1H-NMR spectrum of compound 5 (CD3OD, 400 MHz). ………..….....149 Figure 89. 13C-NMR spectrum of compound 5 (CD3OD, 50 MHz). ………..…......150 Figure 90. COSY (1) spectrum of compound 5 (CD3OD, 400 MHz). ………..……151 Figure 91. COSY (2) spectrum of compound 5 (CD3OD, 400 MHz). ………..…....152 Figure 92. NOESY spectrum of compound 5 (CD3OD, 400 MHz). ………..……...153 Figure 93. HMQC spectrum of compound 5 (CD3OD, 400 MHz). ………..……....154 Figure 94. HMBC spectrum of compound 5 (CD3OD, 400 MHz). ………..……....155 Figure 95. 1H-NMR spectrum of compound 6 (CD3OD, 400 MHz). …………..….156 Figure 96. 13C-NMR spectrum of compound 6 (CD3OD, 100 MHz). ………..…....157 Figure 97. COSY (1) spectrum of compound 6 (CD3OD, 400 MHz). ………..……158 Figure 98. COSY (2) spectrum of compound 6 (CD3OD, 400 MHz). ………..……159 Figure 99. NOESY spectrum of compound 6 (CD3OD, 400 MHz). ………..……...160 Figure 100. HMQC spectrum of compound 6 (CD3OD, 400 MHz). ……..……......161 Figure 101. HMBC (1) spectrum of compound 6 (CD3OD, 400 MHz). ……..….....162 Figure 102. HMBC (2) spectrum of compound 6 (CD3OD, 400 MHz). ……..….....163 Figure 103. 1H-NMR spectrum of compound 7 (CD3OD, 400 MHz). ……..……...164 Figure 104. 13C-NMR spectrum of compound 7 (CD3OD, 50 MHz). …..…..….….165 Figure 105. 1H-NMR spectrum of compound 8 (CD3OD, 400 MHz). ……..….…..166 Figure 106. 13C-NMR spectrum of compound 8 (CD3OD, 100 MHz). ……………167 | |
| dc.language.iso | zh-TW | |
| dc.subject | 活性測試 | zh_TW |
| dc.subject | 類黃酮 | zh_TW |
| dc.subject | 大葉楠 | zh_TW |
| dc.subject | 楨楠屬 | zh_TW |
| dc.subject | 樟科 | zh_TW |
| dc.subject | 甲型葡萄糖水解酶 | zh_TW |
| dc.subject | bioassay | en |
| dc.subject | α-glucosidase | en |
| dc.subject | flavonol | en |
| dc.subject | flavone | en |
| dc.subject | Lauraceae | en |
| dc.subject | Machilus japonica Sieb. ＆ Zucc. var. kusanoi (Hayata) Liao | en |
| dc.title | 大葉楠類黃酮成分之研究 | zh_TW |
| dc.title | Study on the Flavonoid Constituents of Machilus japonica
Sieb. ＆ Zucc. var. kusanoi (Hayata) Liao | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳春雄,林雲蓮,徐鳳麟 | |
| dc.subject.keyword | 活性測試,甲型葡萄糖水解酶,樟科,楨楠屬,大葉楠,類黃酮, | zh_TW |
| dc.subject.keyword | bioassay,α-glucosidase,Machilus japonica Sieb. ＆ Zucc. var. kusanoi (Hayata) Liao,Lauraceae,flavone,flavonol, | en |
| dc.relation.page | 167 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-30 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 7.09 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。