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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾顯雄(Shean-Shong Tzean) | |
dc.contributor.author | Yu-Chen Liu | en |
dc.contributor.author | 劉宇真 | zh_TW |
dc.date.accessioned | 2021-06-13T00:01:14Z | - |
dc.date.available | 2012-08-28 | |
dc.date.copyright | 2007-08-28 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-31 | |
dc.identifier.citation | 王惠芳。1996。靈芝屬含錳超氧歧化{23406a}基因之研究。國立台灣大學農業化學研究所碩士論文。
白佳真。2003。樟芝免疫調節蛋白與超氧歧化酶之研究。國立台灣海洋大學生物科技研究所碩士論文。 吳德鵬。1995。樟芝微量成分的研究。國立台灣師範大學化學研究所碩士論文。 高曉薇。1991。台灣靈芝屬新種樟芝之三萜類成分研究。台北醫學院天然物醫學研究所碩士論文。 陳良坤。2006。牛樟芝萜類生合成基因之選殖及其特性界定。國立臺灣大學植物病理與微生物學硏究所碩士論文。 張怡婷。2004。樟芝抗腫瘤活性之研究。國立成功大學藥理學研究所碩士論文。 張中姿。2002。樟芝菌絲體之甲醇萃取部分對人類肝癌細胞株(HepG2)生長抑制作用的機轉探討。國立台灣大學醫學院生物化學暨分子生物學研究所碩士論文。 程一華。1994。樟芝之成分研究。國立台灣師範大學化學研究所碩士論文。 楊書威。1990。中藥樟菇活性成分之研究。國立台灣大學藥學研究所碩士論文。 曹巧吟。2003。樟芝中免疫調節蛋白的純化與其生理活性之探討。國立台灣大學園藝學研究所碩士論文。 童凱鴻。2005。牛樟芝萜類生理活性探討及其生合成相關基因之分析。國立台灣大學植物病理與微生物學研究所碩士論文。 黃鈴娟。1999。樟芝與姬松茸之抗氧化性質及其多醣組成分析。中興大學食品科學系碩士論文。 葉志新。1996。靈芝屬含錳型超氧歧化酵素的純化與定性。國立台灣大學農業化學研究所碩士論文。 蔡雁暉。2002。樟芝深層培養液及其多醣體之抗氧化特性。國立中興大學食品科學系研究所碩士論文。 盧祉彤。2004。牛樟芝菌體對腫瘤細胞的影響。南台科技大學生物科技研究所碩士論文。 賴鈺菁。2004。樟芝發酵液之抗發炎及其誘導癌細胞凋亡機制之探討。中國醫藥大學營養研究所碩士論文。 Alscher, R. G., Erturk, N., and Heath, L. S. 2002. Role of superoxide dismutase (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53(372): 1331-1341. Altschul, A. M., Abrams, R., and Hogness, T. R. 1940. Cytochrome c peroxidase. J. Biol. Chem. 241:2983-2984. Arthur, J. R. 2000. The glutathione peroxidases. CMLS, Cell. Mol. Life Sci. 57: 1825-1835. Aruoma, O. I. 1994. Nutrition and health aspects of free radicals and antioxidant. Food Chem. Toxic. 32: 671-683. Bannister, J. V., Bannister, W. H., and Rotilio, G. 1987. Aspects of the structure, function, and applications of superoxide dismutase. CRC Crit. Rev. Biochem. 22:111-180. Bannister, W. H., Bannister J. V., Barra D., Bond, J., and Bossa F. 1991. Evolutionary aspects of superoxide dismutase: the copper/zinc enzyme. Free Radical Res.12-13, 349-361. Bechtold, R., and Bosshard, H. R. 1985. Structure of an electron transfer complex II Chemical modification of carboxyl groupsof cytochrome c peroxidase in presence and absence of cytochrome c. J. Biol. Chem..260(8): 5191-5200. Blokhina, O., Virolainen, E., and Fagerstedt, K. V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot. 91: 179-194. Bosshard, H. R., Bänziger J., Hasler, T., and Poulos, T. L. 1984. The cytochrome c peroxidase-cytochrome c electron transfer complex-The role of histidine residues. J. Biol. Chem. 259(9): 5683-5690. Brigelius-Flohé, R., Aumann, K. D., Blöcker, H., Gross, G., Kiess, M., Klöppel, K. D., Maiorino, M., Roveri, A., Schuckelt, R., Ursini, F., Wingender, E., and Flohé, L. 1994. Phospholipid-hydroperoxide glutathione peroxidase. J. Biol. Chem.. 269(10):7342-7348. Bryant, D., Cummins, I., Dixon, D. P., and Edwards, R. 2006. Cloning and characterization of a theta class glutathione transferase from the potato pathogen Phytophthora infestans. Phytochemistry. 67: 1427-1434. Burns, C., Geraghty, R., Neville, C., Murphy, A., Kavanagh, K., and Doyle, S. 2005. Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus. Fungal Genet. Biol. 42: 319-327. Chang, E. C., Crawford, B. F., Hong, Z., Bilinski, T., and Kosman, D. J. 1991. Genetic and biochemical characterization of Cu,Zn superoxide dismutase mutants in Saccharomyces cerevisiae. J. Biol. Chem. 266(7): 4417-4424. Chang, T. T. and W. N. Chou. 2004. Antrodia cinnamomea reconsidered and A. salmonea sp. nov. on Cunninghamia konishii in Taiwan. Bot. Bull. Acad. Sin. 45: 347-352. Chang, T. T. and W. N. Chou. 1995. Antrodia cinnamomea sp. nov. on Cinnamomum kanehiral in Taiwan. Mycol. Res. 99: 756-758. Chen, C. C., Shiao, Y. J., Lin, R. D., Shao, Y. Y., Lai, M. N., Lin, C. C., Ng, L. T., and Kuo, Y. H. 2006. Neuroprotective diterpenes from the fruiting body of Antrodia camphorate. J. Nat.Prod. 69:689-691. Chen, C. H., Yang, S. W., and Shen, Y. C. 1995. New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. J. Nat. Prod. 58(11):1655-1661. Chen, S. C., Lu, M. K., Cheng, J. J., and Wang D. L. 2005. Antiangiogenic activities of polysaccharides isolated from medicinal fungi. FEMS Microbiol. Lett. 249:247-254. Cheng, J. J., Huang, N. K., Chang, T. T., Wang, D. L., and Lu, M. K. 2005. Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sci.. 76:3029-3042. Cherng, I. H., Chiang, H. C., Cheng, M. C., and Wang Y. 1995. Three new triterpenoids from Antrodia cinnamomea. J. Nat. Prod. 58(3):365-371. Collinson, L. P., and Dawes, I. W. 1995. Isolation, characterization and overexpression of the yeast gene, GLR1, encoding glutathione reductase. Gene. 156:123-127. Combet C., Blanchet C., Geourjon C. and Deléage G. 2000. NPS@: Network Protein Sequence Analysis. TIBS. 25(3):147-150. Dym, O. and Eisenberg, D. 2001. Sequence-structure analysis of FAD-containing proteins. Protein Sci. 10:1712-1728. Editorial. 2005. SOD, oxidative stress and human pathologies: a brief history and a future vision. Biomed. Pharmacother. 59: 139-142. Erman, J. E., and Vitello, L. B. 2002. Yeast cytochrome c peroxidase: mechanistic studies via protein engineering. Biochim. Biophys. Acta. 1957:193-220. Ermler, U., and Schulz, G. E. 1991. The three-dimensional structure of glutathione reductase from Escherichia coli at 3.0 A resolution. Proteins. 9(3):174-9. Forstrom J. W., and Tappel, A. L. 1979. Donor substrate specificity and thiol reduction of glutathione disulfide peroxidase. J. Biol. Chem. 254(8): 2888-2891. Fridovich, I. 1995a. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64: 97-112. Fridovich, I. 1986b. Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247(1): 1-11. Gietz D., St. Jean A.Woods R. A., and Schiestl R. H. 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res.. 20(6): 1425. Ghisla, S., and Massev, V. 1989. Mechanisms of flavoprotein-catalyzed reactions. Eur J Biochem. 181(1):1-1. Giles, S. S., Perfect, J. R., and Cox, G. M. 2005. Cytochrome c peroxidase contributes to the antioxidant defense of Cryptococcus neoformans. Fungal Genet. Biol. 42:20-29. Gupta, S., Singh, B., and Sharma, S. C. 1996. Glutathione-glutathione reductase system and lipid peroxidation in Saccharomyces cerevisiae under alcohol stress. Acta Microbiol. Immunol. Hung. 43:33-38. Habig, W. H., Pabst, M. J., and Jakoby, W. B. 1974. Glutathione S-transferases, the first enzymatic step in mercapturic acid formation. J. Biol. Chem.. 249(22):7130-7139. Han, H. F., Nakamura N., Zuo, F., Hirakawa, A., Yokozawa, T., and Hattori, M. 2006. Protective effects of a neutral polysaccharide isolated from the mycelium of Antrodia cinnamomea on Propionibacterium acnes and lipopolysaccharide induced hepatic injury in mice. Chem. Pharm. Bull. 54(4)496-500. Halliwell, B. 1993. The role of oxygen radicals in human disease, with particular reference to the vascular system. Haemostasis. 23(suppl 1):118-126. Hemández-Saavedra N. Y. 2003. Cu,Zn superoxide dismutase in Rhodotorula and Udeniomyces spp. isolated from sea water: cloning and sequencing the encoding region. Yeast. 20: 479-492. Huang, J. K., Ken, C. F., Huang, H. M., and Lin, C. T. 2006. Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata. Appl Microbiol Biotechnol. 74(1):84-92. Huang, L. C., Huang, S. J., Chen, C. C., and Mau, J. L. 1999. Antioxidant properties of Antrodia camphorata. The 3rd ICMBMP. Hsiao, G., Shen, M. Y., Lin, K. H., Lan, M. H., Wu, L. Y., Chou, D. S., Lin, C. H., Su, C. H., and Sheu, J. R. 2003. Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J. Agric. Food Chem. 51:3302-3308. Hseu, Y. C., Wu, F. Y., Wu, J. J., Chen, J. Y., Chang, W. H., Lu, F. J., Lai, Y. C., and Yang, H. L. 2005a. Anti-inflammatory potential of Antrodia camphorata through inhibition of iNOS, COX-2, and cytokines via the NF-κB pathway. International Immunopharmaclogy. 5:1914-1925. Hseu, Y. C., Chang, W. C., Hseu, Y. T., Lee, C. Y., Yech, Y. J., Chen, P. C., Chen, J. Y., and Yang, H. L. 2002b. Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life Sci. 71:469-482. Hwang, C. S., Baek, Y. U., Yim, H. S., and Kang, S. O. 2003a. Protective roles of mitochondrial manganese-containing superoxide dismutase against various stresses in Candida albicans. Yeast. 20: 929-941. Hwang, C. S., Rhie, G. E., Oh, J. H., Huh, W. K., Yim, H. S., and Kang, S. O. 2002b. Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence. Microbiology. 148:3705-3713. Inoue, M., Sato, E. F., Nishikawa, M., Hiramoto, K., Kashiwagi, A., and Utsumi, K. 2004. Free radical theory of apoptosis and metamorphosis. Redox Report. 9(5):237-247. Inoue, Y., Matsuda, T., Sugiyama, K. I., Izawa, S., and Kimura, A. 1999. Genetic analysis of glutathione peroxidase in oxidative stress response of Saccharomyces cerevisiae. J. Biol. Chem.. 274(38): 27002-27009. István P., Prade, R. A., and Penninckx, M. J. 2004. Glutathione, altruistic metabolite in fungi. Adv. Microb. Physiol. 49:1-76. Ito H., Fukuda Y., Murata K., and Kimura A. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153(1):163-168. Jacob, R. A. 1994. Nutrition, health and antioxidants. INFORM 5:1271-1275. Keele, B. B. Jr., McCord, J. M., and Fridovich, I. 1970. Superoxide dismutase from Escherichia coli B. A new enzyme Manganese enzyme. J. Biol. Chem. 245(22):6176-6181. Kendall B. Wallace. 1997. Free radical toxicology. Taylor & Francis. Koehler, C. M., Beverly, K. N., and Leverich, E. P. 2006. Redox pathways of the mitochondrion. Antioxid. Redox Signal. 8(5&6): 813-822. Krauth-Siegel, R. L., Blatterspiel, R., Saleh, M., Schiltz, E., Schirmer, R. H., and Untucht-Grau, R. 1982. Glutathione reductase from human erythrocytes. Eur. J. Biochem. 121:259-267. Kuo, P. L., Hsu, Y. L., Cho, C. Y., Ng, L. T., Kuo, Y. H., and Lin, C. C. 2006. Apoptotic effects of Antrodia cinnamomea fruiting bodies extract are mediated through calcium and calpain-dependent pathways in Hep 3B cells. Food Chem. Toxicol. 44:1316-1326. Lee, I. H., Huang, R. L., Chen, C. T., Chen, H. C., Hsu, W. C., and Lu M. K. 2002. Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol. Lett. 209:63-67. Lee, J., Dawes, I. W., and Roe, J. H. 1997. Isolation, expression, and regulation of the pgr1+ gene encoding glutathione reductase absolutely required for the growth of Schizosaccharomyces pombe. J. Biol. Chem.. 272(37):23042-23049. Lin, M. T., Kuo, T. J., and Lin, C. T. 1998. Molecular cloning of a cDNA encoding copper/zinc superoxide dismutase from papaya fruit and overexpression in Escherichia coli. J. Agric. Food Chem. 46:344-348. Lin, W. C., Kuo, S. C., Lin, W. L., Fang, H. L., and Wang B. C. 2006a. Filtrate of fermented mycelia from Antrodia camphorate reduces liver fibrosis induced by carbon tetrachloride in rats. World J. Gastroenterol. 21;12(15):2369-2374. Lin, W. C., Kuo, S. C., and Wu, Y. W. 2001b. Effects of 28-days repeated oral administration of the fermented extract of mycelia of Antrodia camphorata(CCRC 93032) on rats. J Chin Med. 12(4): 293-303. Liu, D. Z., Liang, Y. C., Lin, S. Y., Lin, Y. S., Wu, W. C., Hou, W. C., and Su, C. H. 2007. Antihypertensive activities of a solid-state culture of Taiwanofungus camphoratus (Chang-Chih) in spontaneously hypertensive rats. Biosci. Biotechnol. Biochem., 71(1): 23-30. Mann, T., and Keilin, D. 1939. Haemocuprin and hepatocuprin, copper-proein compounds of blood and liver in mammals. Proc. R. Soc. London Ser B. 128: 303-304. Mau, J. L., Huang, P. N., Huang, S. J., and Chen .C. C. 2004. Antioxidant properties of methanolic extracts from two kinds of Antrodia camphorata mycelia. Food Chem. 86:25-31. McCord, J. M., and Fridovich, I. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6052. McDongh, O. B., Gralla, E. B., and Valentine, J. S. 1988. The copper, zinc-superoxide dismutase gene of Saccharomyces cerevisiae: cloning, sequencing, and biological activity. Proc. Natl. Acad. Sci. 85:4789-4793. Mei, H., Wang, K., Peffer, N., Weatherly, G., Cohen, D. S., Miller, M., Pielak, G., Durham, B., and Millett, F. 1999. Role of configurational gating in intracomplex electron transfer from cytochrome c to the radical cation in cytochrome c peroxidase. Biochemistry. 38:6846-6854. Meister, A. and Anderson, M. E. 1983. Glutathione. Annu. Rev. Biochem. 52, 711-760. Mills, G. C. 1958b. Purification and properties of glutathione peroxidase of erythrocytes. J. Biol. Chem.. 234(3): 502-506. Mills, G. C. 1957a. Hemoglobin catabolism I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown. J. Biol. Chem.. 229:189-197. Missall, T. A., Cerry-Harris, J. F., and Lodge, J. K. 2005. Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific subtrates. Microbiology. 151: 2573-2581. Mumberg D., Müller R., and Funk M. 1995. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene. 156:119-122. Nagami, H., Umakoshi, H., Shimanouchi, T., and Kuboi, R. 2004. Variable SOD-like activity of liposome modified with Mn(II)-porphyrin derivative complex. Biochem. Eng. J. 21:221-227. Nakamura, N., Hirakawa, A., Gao, J. J., Kakuda, H., Shiro, M., Komatsu, Y., Sheu, C. C., and Hattori, M. 2004. Five new maleic and succinic acid derivatives from the mycelium of Antrodia camphorata and their cytotoxic effects on LLC tumor cell line. J. Nat. Prod. 67, 46-48. Nakani, S., Vitello, L. B., and Erman, J. E. 2006a. Characterization of four covalently-linked yeast cytochrome c/cytochrome c peroxidase complexes: evidence for electrostatic interaction between bound cytochrome c molecules. Biochemistry. 45:14371-14378. Nakani, S., Viriyakul, T., Mitchell, R., Vitello, L. B., and Erman, J. E. 2006b. Charaterization of a covalently linked yeast cytochrome c-cytochrome c peroxidase complex: evidence for a single, catalytically active cytochrome c binding site on cytochrome c peroxidase. Biochemistry. 45:9887-9893. Narasipura, S. D., Ault, J. G., Behr, M. J., Chaturvedi, V., and Chaturvedi, S. 2003. Characterization of Cu,Zn superoxide dismutase (SOD1) gene knock-out mutant of Cryptococcus neoformans var. gattii: role in biology and virulence. Mol. Microbiol. 47(6): 1681-1694. Oakley, A. J. 2005. Glutathione transferases: new functions. Curr. Opin. Struct. Biol. 15:716-723. Orna, C. H., and Storz, G. 2000. Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stress. Annu. Rev. Microbiol. 54:439-461. Outten, C. E., and Culotta, V. C. 2004. Alternative start sites in the Saccharomyces cerevisiae GLR1 gene are responsible for mitochondrial and cytosolic isoforms of glutathione reductase. J. Biol. Chem.. 279(9):7785-7791. Pan, S. M., Hwang, G. B., and Liu, H. C. 1999. Overexpression of rice CuZnSOD in E. coli. Bot. Bull. Acad. Sin. 40:275-281. Park, N. S., Lee, R. S., Sohn, H. D., and Kim, D. H. 2005. Molecular cloning, expression, and characterization of the Cu,Zn superoxide dismutase (SOD1) gene from the entomopathogenic fungus Cordyceps militaris. Mycologia. 97(1): 130-138. Peng, C. C., Chen, K. C., Peng, R. Y., Su, C. H., and Hsieh-Li, H. M. 2006. Human urinary bladder cancer T24 cells are susceptible to the Antrodia camphorata extracts. Cancer Lett. 243,109-119. Penninckx, M. J., and Elsken, M. T. 1993. Metabolism and functions of glutathione in micro-organisms. Adv. Microb. Physiol. 34:239-301. Pfister, T. D., Gengenbach, A. J., Syn, S., and Lu, Y. 2001. The role of redox-active amino acids on compound I stability, substrate oxidation, and protein cross-linking in yeast cytochrome c peroxidase. Biochemistry. 40:14942-14951. Pócsi I., Prade, R. A., and Penninckx, M. J. 2004. Glutathione, altruistic metabolite in fungi. Adv. Microb. Physiol. 49:2-76. Poulos T. L., and Kraut, J. 1980a. A hypothetical model of eht cytochrome c peroxidase˙cytochrome c electron transfer complex. J. Biol. Chem.. 255(21): 10322-10330. Poulos, T. L., Freer, S. T., Xuong, A. N., Edwards, S. L., Hamlin, R. C., and Kraut, J. 1978b. Crystallographic determination of the heme orientation and location of the cyanide binding site in yeast cytochrome c peroxidase. J. Biol. Chem. 253:3730-3735. Rietveld, P., Arscott L. D., Berry, A., Scrution N. S., Deonarain, M. P., Perham, R. N., and Williams C. H. Jr. 1994. Reductive and oxidative half-reactions of glutathione reductase from Escherichia coli. Biochemistry. 22;33(46)13888-133895. Sakamoto, A., Ohsuga, H., and Tanaka, K. 1992. Nucleotide sequences of two cDNA clones encoding different Cu/Zn-superoxide dismutase expressed in developing rice seed(Oryza sativa L.). Plant Mol. Biol. 19:323-327. Schulz, G. E., Schirmer, R. H., Sachsenheimer, W., and Pai, E. F. 1978. The structure of the flavoenzyme glutathione reductase. Nature. 343:38-43. Sheehan, D., Meade, G., Foley, V. M., and Dowd, C. A. 2001. Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochem. J. 360:1-16. Shiao, M. S., and Lin, L. J. 1987. Two new triterpenes of the fungus Ganoderma lucidum. J. Nat. Prod. 50(5):886-890. Shin, Y. H., Park, E. H., Fuchs, J. A., and Lim, C. J. 2002. Characterization, expression and regulation of a third gene encoding glutathione S-transferase from the fission yeast. Biochim. Biophys. Acta. 1577:164-170. Sies, H., 1993. Strategies of antioxidant defense. Eur. J. Biochem. 215:213-219. Song, T. Y., and Yen, G. C. 2002a. Antioxidant properties of Antrodia camphorata in submerged culture. J. Agric. Food Chem. 50:3322-3327. Song, T. Y., and Yen, G. C. 2003b. Protective effects of fermented filtrate from Antrodia camphorata in submerged culture against CCl4-induced hepatic toxicity in rats. J. Agric. Food Chem. 51:1571-1577. Song, T. Y., Hsu, S. L., Yeh, C. T., and Yen, G. C. 2005c. Mycelia from Antrodia camphorata in submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of Fas pathway. J. Agric. Food Chem.53, 5550-5564. Southorn, P. A., and Powis, G. 1988a. Free radicals in medicine. I. Chemical nature and biologic reactions. Mayo Clin Proc. 63(4)381-389. Southorn, P. A., and Powis, G. 1988b. Free radicals in medicine. II. Involvement in human disease. Mayo Clin Proc. 63(4)390-408. Tanaka, T., Izawa, S., and Inoue, Y. 2005. GPX2, encoding a phospholipids hydroperoxide glutathione peroxidase homologue, codes for an atypical 2-Cys peroxiredoxin in Saccharomyces cerevisiae. J. Biol. Chem.. 280(51): 42078-42087. Tsaprailis, G., and English, A. M. 2003. Different pathways of radical translocation in yeast cytochrome c peroxidase and its W191F mutant on reaction with H2O2 suggest an antioxidant role. J. Biol Inorg Chem. 8:248-255. Tutic, M., Lu, X. A., Schirmer, R. H., and Werner, D. 1990. Cloning and sequencing of mammalian glutathione reductase cDNA. Eur. J. Biochem. 188:523-528. Untucht-Grau, R., Schirmer, R. H., Schirmer, I, and Krauth-Siegel R. L. 1981. Glutathione reductase from human erythrocytes: amino-acid sequence of the structurally known FAD-binding domain. Eur J Biochem. 120(2):407-419. Veal, E. A., Toone, W. M., Jones, N., and Morgan, B. A. 2002. Distinct roles for glutathione S-transferase in the oxidative stress response in Schizosaccharomyces pombe. J. Biol. Chem.. 277(38): 35523-35531. Vincent, M., and Charles H. W, JR. 1965. On the Reaction mechanism of yeast glutathione reductase. J. Biol. Chem.. 240(11):4470-4480. Waldmeyer, B., Bechtold, R., Bosshard, H. R., and Poulos, T. L..1982. The cytochrome c peroxidase˙cytochrome c electron transfer complex- Experimental support of hypothetical model. J. Biol. Chem.. 257(11): 6073-6076. Wallace, K. B. 1997. Target organ toxicology series- Free radical toxicology. Taylor & Francis. pp.141-170. Wang, Z. S., He, Z. J., Shen, Q., Gu, Y. X., Li, S. X., and Yuan, Q. S. 2005. Purification and partial characterization of recombinant Cu, Zn containing superoxide dismutase of Cordyceps militaris in E. coli. J. Chromatogr. B. 826:114-121. Wen, L., Huang, H. M., Juang, R. H., and Lin, C. T. 2007. Biochemical characterization of 1-Cys peroxiredoxin from Antrodia camphorata. Appl Microbiol Biotechnol. 73:1314-1322. Williams, C. H. 1976. In “The Enzymes” (P. D. Boyer, ed). Academic Press. 13:89-132. Wongsantichon J., and Ketterman, A. J. 2005. Alternative splicing of glutathione S-transferases. Meth. Enzymol. 401:100-113. Wu, S. H., Yu, Z. H., Dai, Y. C., Chen C. T., Su, C. H., Chen, L. C., Hsu, W. C., and Hwang, G. Y. 2004. Taiwanofungus, a polypore new genus. Fungal Sci. 19(3-4): 109-116. Wu, S. H., L. Ryvarden, and T. T. Chang. 1997. Antrodia camphoratum(“niu-chang-chih”), new combination of a medicinal fungus in Taiwan. Bot. Bull. Acad. Sin. 38: 273-275. Yamada, K., Nakagawa, C. W., and Mutoh, N. 1999. Schizosaccharomyces pombe homologue of glutathione peroxidase, which does not contain selenocysteine, is induced by several stresses and works as an antioxidant. Yeast. 15: 1125-1132. Yang, H. L., Chen, C. S., Chang, W. H., Lu, F. J., Lai, Y. C., Chen, C. C., Hseu, T. H., Kuo, C. T., and Hseu, Y. C. 2006. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett. 231:215-227. Yonetani, T., and Schleyer, H. 1967. Studies on cytochrome c peroxidase IX. The reaction of ferrimyoglobin with hydroperoxides and a comparison of peroxide-induced compounds of ferrimyoglobin and cytochrome c peroxidase. J. Biol. Chem.. 242(8): 1974-1979. Yost Jr F. J., and Fridovich, I. 1973. An iron-containing superoxide dismutase from Escherichia coli. J. Biol. Chem. 248: 4905-4908. Zang, H., He, S., and Mauk, A. G. 2002. Radical formation at Tyr39 and Tyr153 following reaction of yeast cytochrome c peroxidase with hydrogen peroxide. Biochemistry. 41:13507-13513. Zang, M. and C. H. Su. 1990. Ganoderma comphoratum, a new taxon in genus Ganoderma from Taiwan. Acta Bot. Yunnanica 12: 393-396 Zelko, I. N., Mariani, T. J., and Folz, R. J. 2002. Superoxide dismutase multigene family: a comparison of the CuZn-SOD(SOD1), Mn-SOD(SOD2), and EC-SOD(SOD3) gene structures, evolution, and expression. Free Radic. Biol. Med. 33(3): 337-349. Zemlyak, I., Nimon, V., Brooke, S., Moore, T., McLaughlin, J., and Sapolsky, R. 2006. Gene therapy in the nervous system with superoxide dismutase. Brain Res. 1088: 12-18. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28115 | - |
dc.description.abstract | 牛樟芝(Antrodia cinnamomea)為台灣特有之平伏型擔子菌,生長於台灣特有種牛樟樹之樹心空胴內壁,民間盛傳此菇具多重生理活性,抗氧化即為其中之一。為藉由分子層次闡明其抗氧化機制,遂進行篩檢應用真菌研究室所建構、註解之牛樟芝cDNA基因資料庫,由尋獲之五個和抗氧化相關之基因片段EST設計引子對去選殖Cu-Zn superoxide dismutase,Glutathione reductase,Glutathione peroxidase,Glutathione transferase,以及Cytochrome c peroxidase等基因。利用RACE (rapid amplification of cDNA ends)求取此等基因之cDNA全長,以及將對此等基因探針呈雜合反應之Fosmid clones進行primer walking定序,以求取其gDNA序列。已選殖出Cu-Zn superoxide dismutase以及Cytochrome c peroxidase基因之全長度cDNA,前者長度為940 bp,其ORF為591 bp,具3個intron;後者長度為1456 bp,其ORF為1116 bp,具7個intron。而所選殖之glutathione peroxidase其cDNA長為794 bp,ORF為477 bp,具4個intron;glutathione transferase其cDNA長為727 bp,ORF為456 bp,具6個intron;glutathione reductase其cDNA長為794 bp,ORF為453 bp。此三個基因和牛樟芝之EST及gDNA相比,分別可能有20,129,或500 bp之鹼基對尚未被求出。此等基因之生化特性亦經由相關之生物資訊網站加以分析。Cu-Zn SOD已建構於E. coli表現載體pQE 31,但表現不佳;Glutathione transferase也已將其建構於Saccharomyces cerevisiae之互補表現載體p426ADH,以相對之酵母菌S. c.突變株進行互補,亦尚未獲得成功之轉型株,未來將再繼續探討。此外,也利用Virtual Northern(VN)及Q-PCR比較此五個抗氧化基因於牛樟芝菌絲體或子實體之相對表現。一般而言,結果顯示菌絲之表現高於子實體;而分別就不同基因,以VN之結果分析,相對表現量之大小順序為SOD>GP>CYT>GTR>GR;而就Q-PCR結果分析,則為SOD>GTR>CYT>GR>GP。 | zh_TW |
dc.description.abstract | Antrodia cinnamomea (Neu-Chang-Tsu), an endemic resupinate basidiomycetes of Taiwan, inhabitated on the inner cavity of the endemic broad leaved tree, Cinnamomum camphoratum. The claimed potent medicinal activities, including of antioxidation, by A. cinnamomea were well recognized. Attempt to elucidate the antioxidation mechanism molecularly, experiments were initiated by blasting of the previously annotated A. cinnamomea cDNA library. Of the five ESTs, respectively encoding Cu-Zn superoxide dismutase, glutathione reductase, glutathione peroxidase, glutathione transferase, and cytochrome c peroxidase were accessed and cloned by the derived primers. Full-length cDNA of the genes were resolved by RACE (rapid amplification of cDNA ends). Moreover, the gDNA sequences corresponding to these five genes were gained by primer walking from the Fosmid clones shown positive hybridization signals against the DIG-labeled probes. The UTR, introns, active sites, conserved domains, and biochemical properties, etc. were defined by relevant bioinformatics websites. The resolved full-length of Cu-Zn SOD and cytochrome c peroxidase , the former consisting of 940 bp, with 561 bp ORF, 3 introns; while the later composed of 1456 bp, with 1116 bp ORF, and 7 introns.While the cDNA of glutathione peroxidase were 794 bp, 477 bp ORF, and 4 introns; glutathione transferase cDNA 727 bp, ORF 453 bp, and 6 introns; glutathione reductase cDNA 794 bp, ORF 453 bp, no intron. Compared to the EST or gDNA, presumably 20, 129, or 500 bp nucleotides of the three genes, were unallocated. Additionally, Cu-Zn SOD gene has been constructed in the E. coli expression vector pQE 31, and glutathione transferase gene in Saccharomyces cerevisiae complementation vector p426ADH, both neither expressed nor complemented properly, and need work further. With respect in expression of these five genes in mycelium or fruiting body of A. cinnamomea, virtual Northern(VN) or Q-PCR were performed. In general, the activities of these genes in mycelium were higher than those in fruiting body, by VN with the order SOD>GP>CYT>GTR>GR; while by Q-PCR, SOD>GTR>CYT>GR>GP. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T00:01:14Z (GMT). No. of bitstreams: 1 ntu-96-R93633001-1.pdf: 4246518 bytes, checksum: 0017669c2ed4eac53c800abe68f9ffc6 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 中文摘要------------------------------------------------------------------------------------------- 1
Abstract -------------------------------------------------------------------------------------------- 2 前言------------------------------------------------------------------------------------------------- 4 壹、 前人研究------------------------------------------------------------------------------------- 6 牛樟芝簡介--------------------------------------------------------------------------------------- 6 (一) 分類地位---------------------------------------------------------------------------- 6 (二) 型態特性----------------------------------------------------------------------------- 7 (三) 一般成分分析----------------------------------------------------------------------- 8 (四) 生物功能活性研究--------------------------------------------------------------- 10 (五) 牛樟芝之毒理分析--------------------------------------------------------------- 15 自由基的生成----------------------------------------------------------------------------------- 16 生物抗氧化相關機制-------------------------------------------------------------------------- 17 超氧歧化酶(superoxide dismutase, SOD)-------------------------------------------------- 17 (一) 銅鋅型超氧歧化酶(Cu-Zn SOD)----------------------------------------------- 18 (二) 錳型超氧歧化酶(Mn-SOD)----------------------------------------------------- 19 (三) 鐵型超氧歧化酶(Fe-SOD)------------------------------------------------------ 19 麩胺酸生合成途徑(glutathione synthesis pathway)與相關生化反應----------------- 20 (一) 麩胺酸還原酶(glutathione reductase)------------------------------------------ 21 (二)麩胺酸過氧化酶(glutathione peroxidase)-------------------------------------- 22 (三)麩胺酸轉移酶(glutathione transferase)----------------------------------------- 24 細胞色素c過氧化酶(cytochrome c peroxidase)------------------------------------------- 26貳、材料與方法---------------------------------------------------------------------------------- 28 一、 菌株及其培養----------------------------------------------------------------------- 28 二、 RNA製備---------------------------------------------------------------------------- 28 三、 反轉錄合成cDNA----------------------------------------------------------------- 31 四、 同步定量PCR----------------------------------------------------------------------- 31 五、 虛擬北方氏雜合反應-------------------------------------------------------------- 33 六、 抗氧化相關基因之RACE(Rapid Amplification of cDNA Ends)---------- 40 七、 牛樟芝Fosmid library中抗氧化相關基因之clone篩選及定序------------ 41 八、 Fosmid primer walking ------------------------------------------------------------ 44 九、 利用大腸桿菌表現系統進行基因功能性分析-------------------------------- 45 十、 酵母菌突變株互補實驗進行基因功能性分析-------------------------------- 47 十一、 序列分析 ----------------------------------------------------------------------- 47 參、結果------------------------------------------------------------------------------------------- 49 (一)麩胺酸還原酶 (glutathione reductase) ---------------------------------------- 49 (二)麩胺酸過氧化酶 (glutathione peroxidase) ------------------------------------ 53 (三)麩胺酸轉移酶 (glutathione S-transferase) ------------------------------------ 56 (四)細胞色素c過氧化酶 (cytochrome c peroxidase) --------------------------- 59 (五)超氧歧化酶 (superoxide dismutase) ------------------------------------------- 62 肆、討論------------------------------------------------------------------------------------------- 67 伍、圖表------------------------------------------------------------------------------------------- 74 陸、參考文獻------------------------------------------------------------------------------------ 125 附錄一、實驗試劑配方----------------------------------------------------------------------- 138 附錄二、培養基成分-------------------------------------------------------------------------- 141 附錄三 -------------------------------------------------------------------------------------------142 附錄四 -------------------------------------------------------------------------------------------148 | |
dc.language.iso | zh-TW | |
dc.title | 牛樟芝之抗氧化酶相關基因選殖及其生理活性分析 | zh_TW |
dc.title | Cloning and characterization of antioxidation related genes of Antrodia cinnamomea | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉瑞芬(Ruey-Fen Liou),袁國芳(Kuo-Fan Yuan),葉信宏(Hsin-Hung Yeh),沈湯龍(Tang-Long Shen) | |
dc.subject.keyword | 樟芝,抗氧化,超氧歧化酶,穀胱甘肽,相關酵素, | zh_TW |
dc.subject.keyword | Antrodia cinnamomea,antioxidation,superoxide dismutase,glutathione related enzyme, | en |
dc.relation.page | 151 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-31 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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