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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳蕙芬(Whi Fin Wu) | |
| dc.contributor.author | Kuei-Peng Chen | en |
| dc.contributor.author | 陳圭芃 | zh_TW |
| dc.date.accessioned | 2021-06-12T18:29:21Z | - |
| dc.date.available | 2010-08-28 | |
| dc.date.copyright | 2007-08-28 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-08-03 | |
| dc.identifier.citation | 施如珊 (2004). 大腸桿菌熱休克蛋白ClpY I domain之突變蛋白及其專一性基質辨識之研究. 台大農化所碩士論文.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27944 | - |
| dc.description.abstract | Glutaredoxin是一種普遍存在生物體中的蛋白質，可以藉由glutathione（GSH）催化雙硫鍵還原。大腸桿菌中，Grx4是一個新發現的glutaredoxin，除了Grx4外，還具有其他具雙硫醇活化中心（CPYC）的glutaredoxin（Grx1、Grx2、Grx3），然而，Grx4具有單硫醇的活化中心（CFGS）。Grx4是由grxD基因所轉錄轉譯而來，包含115個胺基酸，分子量為12.7 kDa。在細胞中可以和還原態的GSH合作以消除細胞內產生的氧化壓力。在本研究中，我們嘗試建構grxD基因缺失的突變株（grxD knockout mutant），但無法建構單純具有grxD::kan的突變株，只能得到帶有雙套grxD基因的突變株，但此結果和國外其他研究報告結果互相吻合，因此可以推論，grxD為E. coli中之一必須基因。而在此突變株中，也觀察到其生長速度較野生株來得緩慢，但在加入野生型grxD基因後，生長速度可回復至和野生型相雷同的程度。另外，也以質體大量表現Grx4-6xHis蛋白質產生，並以SDS-PAGE方式，確認其分子量為12.7kDa。最後，我們也發現Grx4和大腸桿菌ClpYQ蛋白中的ClpY部份有交互作用，在後續的ClpY I domain突變株與Grx4蛋白質的酵母菌雙雜交試驗中，也確認了Grx4蛋白質可受到ClpY蛋白的辨認，並與之結合；並可支持「I domain為ClpY蛋白質辨識受質區域」的推論。 | zh_TW |
| dc.description.abstract | Abstract
Glutaredoxins are ubiquitous proteins that catalyze the reduction of disulfides via reduced glutathione (GSH). Escherichia coli has three glutaredoxins (Grx1, Grx2, Grx3), all containing the classical dithiol active site CPYC. In this work, we are focus at Grx4, a newly found glutaredoxin in E. coli, encoded by gene grxD. The protein consists of 115 amino acids (ca. 12.7 kDa), has a potential monothiol (CGFS) active site. The grxD gene was reported as an essential gene in Escherichia coli. In this work, we tried different strategy to make a disruption mutant and suspect that the gene is essential for E. coli; however, only a two-copy “knock out” mutant was obtained. In the growth test, the two-copy “knock out” mutant grew slowly, but after obtaining another wild-type grxD copy, the growth condition was compensated. In the protein purification, a Grx4-6xHis recombination protein was constructed, and then purified with an affinity resin. As examined with SDS-PAGE, the protein size is 12.7kDa, matching the theoretical size. At the last, Grx4 may have an interaction with ClpY protein, a part of protease ClpYQ in E. coli. In this work, the yeast two-hybrid system was performed on the ClpY mutants and Grx4 protein, thus it is likely the result that Grx4 would be a substrate of ClpY protease in vivo, and thus implying that ClpY might recognize and bind the substrate with the I domain. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-12T18:29:21Z (GMT). No. of bitstreams: 1 ntu-96-R92623006-1.pdf: 1297084 bytes, checksum: 74158783b013f278aa2f71d4ee923cd1 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 口試委員審定書 ……………………………………………………………… ii
謝誌 ………………….……………………………………………………… iii 中文摘要 …………………………………………………………………….. iv 英文摘要 …………….………………………………………………………. v 前言 ………………….……………………………………………………… 1 氧化壓力 ……….……………………………………………………… 1 Glutaredoxin系統簡介 ……………………………………………... 2 不同種類的Glutaredoxin ……………………………………………... 3 Glutaredoxin折疊構型 ……………………………………………... 5 Glutaredoxin的催化機制 ……………………………………………... 6 Glutaredoxin蛋白的功能 ……………………………………………... 7 Glutaredoxin系統介紹 ……………………………………………... 9 實驗緣由及目的 ……………………………………………………………… 13 材料與方法 ………………………………………………………………… 14 實驗材料 ……………………………….……………………………… 14 實驗方法 ……………………………….……………………………… 15 一般研究方法 …………...……..………………………………... 15 建構grxD突變株 ………………..……………………………... 20 grxD生長測試 ..………………………………………………... 24 Grx4表現 ………………………………………………………… 25 Grx4交互作用 ..………………………………………………... 32 實驗結果 …………….………………………………………………………. 36 建構grxD knock-out突變株 ………………………………………… 36 測定grxD::kan突變株中生長情況 …………………………………... 36 表現並純化Grx4蛋白質 ……………………………………………. 37 Grx4與ClpY蛋白質間的交互作用 …………………………………... 37 討論 …………………………………………………………………………. 40 Reference …………….………………………………………………………. 43 表 …………………..….……………………………………………………… 49 圖 …………………..….……………………………………………………… 51 附圖 …………………………………………………………………………. 57 | |
| dc.language.iso | zh-TW | |
| dc.title | 大腸桿菌中 Grx4 蛋白質之研究 | zh_TW |
| dc.title | Study of the Grx4 protein in Escherichia coli | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 丁一倪(I-Ni Ting),杜宜殷(Yi-Yin Do) | |
| dc.subject.keyword | 大腸桿菌,氧化壓力, | zh_TW |
| dc.subject.keyword | Escherichia coli,glutaredoxin, | en |
| dc.relation.page | 63 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-08-03 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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