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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李平篤(Ping-Du Lee) | |
| dc.contributor.author | Hou-Ren Lu | en |
| dc.contributor.author | 盧厚任 | zh_TW |
| dc.date.accessioned | 2021-06-12T18:23:53Z | - |
| dc.date.available | 2007-08-28 | |
| dc.date.copyright | 2007-08-28 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-08-15 | |
| dc.identifier.citation | Amir, J., and Preiss, J. 1982. Kinetic Characterization of Spinach Leaf Sucrose-Phosphate Synthase. Plant Physiol. 69(5): 1027-1030.
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Coarse and Fine Control and Annual Changes of Sucrose-Phosphate Synthase in Norway Spruce Needles. Plant Physiol. 112(2): 641-649. Lunn, J.E., and ap Rees, T. 1990. Apparent equilibrium constant and mass-action ratio for sucrose-phosphate synthase in seeds of Pisum sativum. Biochem J. 267(3): 739-43. Lunn, J.E., and ap Rees, T. 1990. Purification and properties of sucrose-phosphate synthase from seeds of Pisum sativum. Phytochemistry 29:1057-1064 Lunn, J.E., Furbank RT, Hatch MD 1997 Adenosine 5'-triphophate-mediated activation of sucrose-phosphate synthase in bundle sheath cell of C4 plant. Planta 202: 249-256 Lunn, J.E., Price, G.D., and Furbank, R.T. 1999. Cloning and expression of a prokaryotic sucrose-phosphate synthase gene from the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol. 40(2): 297-305. McMichael, R.W., Jr., Bachmann, M., and Huber, S.C. 1995. Spinach Leaf Sucrose-Phosphate Synthase and Nitrate Reductase Are Phosphorylated/Inactivated by Multiple Protein Kinases in Vitro. Plant Physiol. 108(3): 1077-1082. McMichael, R.W., Jr., Klein, R.R., Salvucci, M.E., and Huber, S.C. 1993. Identification of the major regulatory phosphorylation site in sucrose-phosphate synthase. Arch Biochem Biophys. 307(2): 248-52. McMichael, R.W., Jr., Kochansky, J., Klein, R.R., and Huber, S.C. 1995. Characterization of the substrate specificity of sucrose-phosphate synthase protein kinase. Arch Biochem Biophys. 321(1): 71-5. Micallef B.J., Haskins K.A. Vanderveer P.J., Roh K., Shewmarker C.K., Sharkey T.D. 1995 Altered photosynthesis, flowering, and fruiting in transgenic tomato plant that have an increased capacity for sucrose synthesis. Planta 196: 327-334 Nguyen-Quoc, B., and Foyer, C.H. 2001. A role for 'futile cycles' involving invertase and sucrose synthase in sucrose metabolism of tomato fruit. J Exp Bot. 52(358): 881-9. 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Plant, Cell & Environment 15; 25-35 Reimholz R, Geigenberger P, Stitt M 1994 Sucrose-phosphate synthase is regulated via metabolites and protein phosphorylation in potato tubers, in a manner analogous to the enzyme in leaves. Planta 192: 480-488 Rorem ES, Walker H.G., McMicheal R.W. 1960 Biosynthesis of sucrose and sucrose-phosphate by sugar beet leaf extracts. Plant Physiol. 35: 269-273 Salerno G.L., Ponit H.G., 1987 Sucrose phosphate synthase. Separation from sucrose synthase and a study of its properties. Planta 142: 41-48 Salvucci, M.E., Drake, R.R., and Haley, B.E. 1990. Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves. Arch Biochem Biophys. 281(2): 212-8. Salvucci, M.E., and Klein, R.R. 1993. Identification of the uridine-binding domain of sucrose-phosphate synthase. Expression of a region of the protein that photoaffinity labels with 5-azidouridine diphosphate-glucose. Plant Physiol. 102(2): 529-36. Schleucher J, Vanderveer P.J., Sharkey T.D. 1998 Exprot of carbon from chloroplasts at night. Plant Physiol. 118: 1439-1445 Sicher, R.C., Kremer, D.F., and Harris, W.G. 1986. Control of Photosynthetic Sucrose Synthesis in Barley Primary Leaves: Role of Fructose 2,6-Bisphosphate. Plant Physiol. 82(1): 15-18. Siegl G, Stitt M 1990 Partial purification of two froms of spinach leaf sucrose-phosphate synthase which differ in their kinetic properties. Plant Science 66: 205-210. Singh M.B., Knox RB 1984 Invertases of Lilium pollen. Characterization and activity during in vitro germination. Plant Physiol. 74: 510-515. Sinha A.K., Pathre U, Sane PV 1997 Purification and characterization of sucrose-phosphate synthase from Prosopis Juliflora. Phytochemistry 46: 441-447. Slabink E, Frydman RB, Cardini C.E., 1968 Some properties of potato tuber UDPG:D-fructose-2-glucosyltransferase (E.C.2.4.1.14) and UDPG:D-fructose-6-phosphate-2- glucosyltransferase (E.C. 2.4.1.13). Plant Physiol. 43: 1036-1068. 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Differential expression of two genes for sucrose-phosphate synthase in sugarcane: molecular cloning of the cDNAs and comparative analysis of gene expression. Plant Cell Physiol. 38(8): 961-5. Toroser, D., Athwal, G.S., and Huber, S.C. 1998. Site-specific regulatory interaction between spinach leaf sucrose-phosphate synthase and 14-3-3 proteins. FEBS Lett. 435(1): 110-4. Toroser, D., and Huber, S.C. 1997. Protein phosphorylation as a mechanism for osmotic-stress activation of sucrose-phosphate synthase in spinach leaves. Plant Physiol. 114(3): 947-55. Trethewey R.N., Rees T.A., 1994 A muntant of Arabidopsis thaliana lacking the ability to transport glucose across the chloroplast envelope. Biochemical Journal 301: 449-454. Usuda, H., Kalt-Torres, W., Kerr, P.S., and Huber, S.C. 1987. Diurnal Changes in Maize Leaf Photosynthesis : II. Levels of Metabolic Intermediates of Sucrose Synthesis and the Regulatory Metabolite Fructose 2,6-Bisphosphate. Plant Physiol. 83(2): 289-293. 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Yang, J., Zhang, J., Wang, Z., and Zhu, Q. 2001. Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling. J Exp Bot. 52(364): 2169-79. Zrenner R, Stitt M 1991 Comparison of the effect of rapidly and gradually developing water-stesson carbohydrate metablism in spinach leaves. Plant, Cell & Environment 14:939-946. 莊榮輝 1985 水稻蔗糖合成酶之研究。國立臺灣大學農業化學研究所博士論文。 鄭宇哲 1997 水稻乳熟期穀實蔗糖磷酯合成酶之生化研究。國立臺灣大學農業化學研究所碩士論文。 陳韋良 1998 甘藷塊根蔗糖磷酯合成酶之生化學研究。國立臺灣大學農業化學研究所碩士論文。 黃瀅如 2000 綠竹筍蔗糖合成酶之純化與生化性質探討。國立臺灣大學農業化學研究所碩士論文。 陳勁中 2001 甘藷葉蔗糖磷酯合成酶之生化學研究。國立臺灣大學農業化學研究所碩士論文。 林秋宏 2001 綠竹筍蔗糖合成酶 cDNA 之選殖與檢定。國立臺灣大學農業化學研究所碩士論文。 曾靖涵 2002 綠竹筍蔗糖磷酯合成酶之生化學研究。國立臺灣大學農業化學研究所碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27848 | - |
| dc.description.abstract | 蔗糖磷酯合成酶 (sucrose phosphate synthase, SPS, EC 2.4.1.14) 是植物蔗糖生合成的重要調控酵素，本論文以綠竹筍之筍殼為材料，進行 SPS 之生化學研究。粗抽液經陰離子交換層析 (DEAE-Sepharose CL-6B chromatography) 後，發現有兩種 SPS 活性分劃，分別經親和層析 ( | zh_TW |
| dc.description.abstract | Sucrose phosphate synthase (SPS, EC 2.4.1.14) is an important regulatory enzyme in sucrose-biosynthetic pathway. In this study, SPS was isolated from bamboo shells and two distinguished isofroms were identified using DEAE-Spharose CL-6B chromatography. The two isoform of SPS were then further purified by 24% PEG precipitation, | en |
| dc.description.provenance | Made available in DSpace on 2021-06-12T18:23:53Z (GMT). No. of bitstreams: 1 ntu-96-R94b47215-1.pdf: 1067079 bytes, checksum: f82a3fdd5caa0d19c892570be268f149 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目 錄
目錄 1 縮寫表 4 中文摘要 5 英文摘要 6 第一章 緒論 7 1.1 蔗糖的生合成與分解 7 1.2 蔗糖的生合成與分解 7 1.3 SPS 的研究背景 9 1.4 SPS 的純化 10 1.5 SPS 特質 10 1.5.1 基質的特異性 10 1.5.2 物理性質及化學特性 11 1.6 SPS 活性調控 11 1.6.1 活性調控與日夜變化的關係 11 1.6.1.1 蔗糖磷酯合成酶激酶 (SPS kinase, SPSK) 11 1.6.1.2 蔗糖磷酯合成酶之蛋白質磷酯酶 (SPS-protein phosphatase) 12 1.6.2 活性調控與滲透逆境的關係 13 1.6.3 活性調控與 Glc 6-P 及 Pi 的關係 14 1.6.4 SPS 分類 14 1.7 SPS 分子生物學研究 14 1.8 SPS 酵素複合體 15 1.9 SPS 在轉殖植物上的研究 16 1.10 實驗緣起 16 第二章 材料與方法 17 2.1 使用材料 17 2.2 蛋白質定量法 17 2.3 SPS 活性測定 18 2.4 SuS 活性測定 20 2.5 電泳檢定系統 21 2.5.1 原態膠體電泳 21 2.5.2 SDS-膠體電泳 23 2.5.3 Coomassie Brilliant Blue R-250 (CBR) 蛋白質染色法 25 2.5.4 膠片乾燥 25 2.6 SPS 之純化 26 2.6.1 酵素粗抽 26 2.6.2 DEAE Sepharoe CL-6B 管柱層析 (Batch) 27 2.6.3 ω-aminohexyl-Sepharose 4B 管柱層析 28 2.6.4 快速蛋白質液相層析 (Fast Protein Liquid Chromatography，FPLC) 29 FPLC 裝置 30 2.6.4.1 Mono Q HR 5/5 chromatography 30 2.6.4.2 Suprose 6 HR 10/30 chromatography 31 2.6.5 膠體過濾法 (Gel-filtraction) 32 2.7 酵素生化性質分析 34 2.7.1 最適反應溫度 34 2.7.2 最適反應時間 34 2.7.3 Vmin/Vmax 比値 35 2.7.4 不同 pH 値對酵素活性的影響 35 2.7.5 不同 Glc-6-P 濃度對酵素活性的影響 35 2.7.6 不同 Pi 濃度對酵素活性的影響 36 2.7.7 SPS之動力學研究 36 2.7.8 Cibacron blue 3GA 對酵素活性的影響 37 第三章 結果與圖表 38 3.1酵素純化方式的探討 38 3.2 綠竹筍之筍殼 SPS 純化結果 38 3.2.1 綠竹筍之筍殼純化之流程 39 3.2.2 粗萃取方法 39 3.2.3 DEAE-Sepharose chromatography (batch) 39 3.2.4.1 w-aminohexyl-Sepharose 4B (200 mM NaCl elution) 40 3.2.4.2 FPLC-Mono Q ( 200 mM elution) 40 3.2.5.1 w-aminohexyl-Sepharose 4B (400 mM NaCl elution) 40 3.2.5.2 FPLC-Mono Q for 400 mM elution 41 3.3 蔗糖磷酯合成酶的生化性質 41 3.3.1 最適反應溫度 41 3.3.2 最適反應時間 41 3.3.3 Vmax / Vlimit 41 3.3.4 pH 的影響 42 3.3.5 Glc 6-P 的影響 42 3.4.6 Pi 的影響 42 3.4.7 酵素動力學研究 42 3.4.8 Cibacron blue 3GA 對 SPS 的影響 43 3.5 SPS2 原態分子量之測定 43 圖表集 44 第四章 結論與展望 79 4.1筍殼 SPS 純化 79 4.2 筍殼 SPS 的確認 79 4.3 SPS 之酵素動力學探討 79 4.3.1 SPS 異構酶之 Vmax/Vlimit 比較 79 4.3.2 SPS 異構酶之最適 pH 79 4.3.3 Glc 6-P 與 Pi 對於 SPS 異構酶之影響 80 4.3.4 Km 與 SPS 異構酶之關係 80 4 4 筍殼純化所得兩個 SPS 為異構酶的確認 80 4.5 未來展望 81 參考文獻 82 | |
| dc.language.iso | zh-TW | |
| dc.title | 綠竹筍殼蔗糖磷酯合成酶之生化學研究 | zh_TW |
| dc.title | Biochemical Studies on Sucrose Phosphate Synthase
from Shell of Bamboo (Bambusa oldhami) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊健志(Chien-Chin Yang),林棋財(Chi-Tsai Lin) | |
| dc.subject.keyword | 蔗糖磷酯合成酶,異構酶, | zh_TW |
| dc.subject.keyword | sps,sucrose phosphate synthase,isozyme, | en |
| dc.relation.page | 87 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-08-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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