請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27059完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃慶璨 | |
| dc.contributor.author | Chun-Yi Kuo | en |
| dc.contributor.author | 郭俊毅 | zh_TW |
| dc.date.accessioned | 2021-06-12T17:54:39Z | - |
| dc.date.available | 2008-02-18 | |
| dc.date.copyright | 2008-02-18 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-02-04 | |
| dc.identifier.citation | 1. Albano, C. R., L. Randers-Eichhorn, W. E. Bentley, and G. Rao. 1998. Green fluorescent protein as a real time quantitative reporter of heterologous protein production. Biotechnol Prog 14:351-4.
2. Antoniou, M., F. Geraghty, J. Hurst, and F. Grosveld. 1998. Efficient 3'-end formation of human beta-globin mRNA in vivo requires sequences within the last intron but occurs independently of the splicing reaction. Nucleic Acids Res 26:721-9. 3. Aoki, T., Y. Usuda, H. Miyakoshi, K. Tamura, and R. B. Herberman. 1987. Low natural killer syndrome: clinical and immunologic features. Nat Immun Cell Growth Regul 6:116-28. 4. Arakawa, T., D. K. Chong, J. L. Merritt, and W. H. Langridge. 1997. Expression of cholera toxin B subunit oligomers in transgenic potato plants. Transgenic Res 6:403-13. 5. Arundhati, A., H. Feiler, J. Traas, H. Zhang, P. A. Lunness, and J. H. Doonan. 1995. A novel Arabidopsis type 1 protein phosphatase is highly expressed in male and female tissues and functionally complements a conditional cell cycle mutant of Aspergillus. Plant J 7:823-34. 6. Baldauf, S. L., and J. D. Palmer. 1993. Animals and fungi are each other's closest relatives: congruent evidence from multiple proteins. Proc Natl Acad Sci U S A 90:11558-62. 7. Barta, A., K. Sommergruber, D. Tompson, K. Hartmuth, M. Matzke, and A. Matzke. 1986. The expression of a nopaline synthase-human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissue. Plant Molecular Biology 6:347-57. 8. Bartholomew, K., G. Dos Santos, T. Dumonceaux, T. Charles, and F. Archibald. 2001. Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble. Appl Microbiol Biotechnol 56:201-4. 9. Bayer, M. E., B. S. Blumberg, and B. Werner. 1968. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis. Nature 218:1057-9. 10. Bitter, G. A., and K. M. Egan. 1988. Expression of interferon-gamma from hybrid yeast GPD promoters containing upstream regulatory sequences from the GAL1-GAL10 intergenic region. Gene 69:193-207. 11. Bok, J. W., E. C. Choi, and B. K. Kim. 1994. Studies on protoplast fusion between Lentinula edodes and Ganoderma lucidum. Arch Pharm Res 17:492-6. 12. Bonetta, L. 2002. Edible vaccines: not quite ready for prime time. Nat Med 8:94. 13. Brown, A. J., and L. A. Casselton. 2001. Mating in mushrooms: increasing the chances but prolonging the affair. Trends Genet 17:393-400. 14. Burns, C., K. E. Gregory, M. Kirby, M. K. Cheung, M. Riquelme, T. J. Elliott, M. P. Challen, A. Bailey, and G. D. Foster. 2005. Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns. Fungal Genet Biol 42:191-9. 15. Burns, C., K. M. Leach, T. J. Elliott, M. P. Challen, G. D. Foster, and A. Bailey. 2006. Evaluation of Agrobacterium-mediated transformation of Agaricus bisporus using a range of promoters linked to hygromycin resistance. Mol Biotechnol 32:129-38. 16. Callis, J., M. Fromm, and V. Walbot. 1987. Introns increase gene expression in cultured maize cells. Genes Dev 1:1183-200. 17. Carrez, D., W. Janssens, P. Degrave, C. A. van den Hondel, J. R. Kinghorn, W. Fiers, and R. Contreras. 1990. Heterologous gene expression by filamentous fungi: secretion of human interleukin-6 by Aspergillus nidulans. Gene 94:147-54. 18. Chakraborty, B. N., N. A. Patterson, and M. Kapoor. 1991. An electroporation-based system for high-efficiency transformation of germinated conidia of filamentous fungi. Can J Microbiol 37:858-63. 19. Chang, M. H., C. J. Chen, M. S. Lai, H. M. Hsu, T. C. Wu, M. S. Kong, D. C. Liang, W. Y. Shau, and D. S. Chen. 1997. Universal hepatitis B vaccination in Taiwan and the incidence of hepatocellular carcinoma in children. N Engl J Med 336:1855-9. 20. Chen, N., M. R. Bowles, and S. M. Pond. 1993. Polyclonal amanitin-specific antibodies: production and cytoprotective properties in vitro. Biochem Pharmacol 46:327-9. 21. Chen, X., M. Stone, C. Schlagnhaufer, and C. P. Romaine. 2000. A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Appl Environ Microbiol 66:4510-3. 22. Combier, J. P., D. Melayah, C. Raffier, G. Gay, and R. Marmeisse. 2003. Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum. FEMS Microbiol Lett 220:141-8. 23. Conesa, A., P. J. Punt, N. van Luijk, and C. A. van den Hondel. 2001. The secretion pathway in filamentous fungi: a biotechnological view. Fungal Genet Biol 33:155-71. 24. Cregg, J. M., T. S. Vedvick, and W. C. Raschke. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11:905-10. 25. Curtiss, R. I., and C. A. Cardineau. 1990. Oral immunisation by transgenic plants. World Patent Application WO 90/02484. 26. Daniell, H., S. J. Streatfield, and K. Wycoff. 2001. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 6:219-26. 27. De Groo, M. J., P. Bundtock, P. J. Hooykaas, and A. G. Beijersbergen. 1998. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16:839-42. 28. De Zoeten, G. A., J. R. Penswick, M. A. Horisberger, P. Ahl, M. Schultze, and T. Hohn. 1989. The expression, localization, and effect of a human interferon in plants. Virology 172:213-22. 29. Dogan, B., H. S. Mason, L. Richter, J. B. Hunter, and M. L. Shuler. 2000. Process options in hepatitis B surface antigen extraction from transgenic potato. Biotechnol Prog 16:435-41. 30. Doring, F., M. Klapper, S. Theis, and H. Daniel. 1998. Use of the glyceraldehyde-3-phosphate dehydrogenase promoter for production of functional mammalian membrane transport proteins in the yeast Pichia pastoris. Biochem Biophys Res Commun 250:531-5. 31. Dunn-Coleman, N. S., P. Bloebaum, R. M. Berka, E. Bodie, N. Robinson, G. Armstrong, M. Ward, M. Przetak, G. L. Carter, R. LaCost, and et al. 1991. Commercial levels of chymosin production by Aspergillus. Biotechnology 9:976-81. 32. Eriksson, P., L. Andre, R. Ansell, A. Blomberg, and L. Adler. 1995. Cloning and characterization of GPD2, a second gene encoding sn- glycerol 3-phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1. Mol Microbiol 17:95-107. 33. Faye, L., A. Boulaflous, M. Benchabane, V. Gomord, and D. Michaud. 2005. Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming. Vaccine 23:1770-8. 34. Fischer, R., and N. Emans. 2000. Molecular farming of pharmaceutical proteins. Transgenic Res 9:279-99; discussion 277. 35. Fox, J. L. 2003. Puzzling industry response to ProdiGene fiasco. Nat Biotechnol 21:3-4. 36. Franken, E., U. Teuschel, and R. Hain. 1997. Recombinant proteins from transgenic plants. Curr Opin Biotechnol 8:411-6. 37. Fujimiya, Y., Y. Suzuki, K. Oshiman, H. Kobori, K. Moriguchi, H. Nakashima, Y. Matumoto, S. Takahara, T. Ebina, and R. Katakura. 1998. Selective tumoricidal effect of soluble proteoglucan extracted from the basidiomycete, Agaricus blazei Murill, mediated via natural killer cell activation and apoptosis. Cancer Immunol Immunother 46:147-59. 38. Ganem, D., and H. E. Varmus. 1987. The molecular biology of the hepatitis B viruses. Annu Rev Biochem 56:651-93. 39. Geli, M. I., M. Torrent, and D. Ludevid. 1994. Two structural domains mediate two sequential events in [gamma]-zein targeting: protein endoplasmic reticulum retention and protein body formation. Plant Cell 6:1911-1922. 40. Glumoff, V., O. Kappeli, A. Fiechter, and J. Reiser. 1989. Genetic transformation of the filamentous yeast, Trichosporon cutaneum, using dominant selection markers. Gene 84:311-8. 41. Gniadkowski, M., M. Hemmings-Mieszczak, U. Klahre, H. X. Liu, and W. Filipowicz. 1996. Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia. Nucleic Acids Res 24:619-27. 42. Godio, R. P., R. Fouces, E. J. Gudina, and J. F. Martin. 2004. Agrobacterium tumefaciens-mediated transformation of the antitumor clavaric acid-producing basidiomycete Hypholoma sublateritium. Curr Genet 46:287-94. 43. Gouka, R. J., P. J. Punt, J. G. Hessing, and C. A. van den Hondel. 1996. Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains. Appl Environ Microbiol 62:1951-7. 44. Granado, J. D., K. Kertesz-Chaloupkova, M. Aebi, and U. Kues. 1997. Restriction enzyme-mediated DNA integration in Coprinus cinereus. Mol Gen Genet 256:28-36. 45. Hanif, M., A. G. Pardo, M. Gorfer, and M. Raudaskoski. 2002. T-DNA transfer and integration in the ectomycorrhizal fungus Suillus bovinus using hygromycin B as a selectable marker. Curr Genet 41:183-8. 46. Haq, T. A., H. S. Mason, J. D. Clements, and C. J. Arntzen. 1995. Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268:714-6. 47. Harmsen, M. C., F. H. Schuren, S. M. Moukha, C. M. van Zuilen, P. J. Punt, and J. G. Wessels. 1992. Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus. Curr Genet 22:447-54. 48. Herzog, R. W., N. K. Singh, C. Schmidt, and P. A. Lemke. 1995. Presence of a P1 bacteriophage sequence in transforming plasmids of Pleurotus ostreatus. Curr Genet 27:460-5. 49. Hiatt, A. 1990. Antibodies produced in plants. Nature 344:469-70. 50. Hileman, B. 2003. Prodigene and Starlink incidents provide ammunition for critics. Chem Eng News 81:23. 51. Hilleman, M. R. 1987. Yeast recombinant hepatitis B vaccine. Infection 15:3-7. 52. Hiraiwa, N., M. Nishimura, and I. Hara-Nishimura. 1997. Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae. Plant J 12:819-29. 53. Hirano, T., T. Sato, K. Okawa, K. Kanda, K. Yaegashi, and H. Enei. 1999. Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Lentinus edodes. Biosci Biotechnol Biochem 63:1223-7. 54. Hirano, T., T. Sato, K. Yaegashi, and H. Enei. 2000. Efficient transformation of the edible basidiomycete Lentinus edodes with a vector using a glyceraldehyde-3-phosphate dehydrogenase promoter to hygromycin B resistance. Mol Gen Genet 263:1047-52. 55. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-9. 56. Holland, M. J., and J. P. Holland. 1978. Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase. Biochemistry 17:4900-7. 57. Honda, Y., T. Matsuyama, T. Irie, T. Watanabe, and M. Kuwahara. 2000. Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus. Curr Genet 37:209-12. 58. Hopwood, D. A. 1981. Genetic studies with bacterial protoplasts. Annu Rev Microbiol 35:237-72. 59. Huang, M. T., and C. M. Gorman. 1990. Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA. Nucleic Acids Res 18:937-47. 60. Huovila, A. P., A. M. Eder, and S. D. Fuller. 1992. Hepatitis B surface antigen assembles in a post-ER, pre-Golgi compartment. J Cell Biol 118:1305-20. 61. Irie, T., Y. Honda, T. Hirano, T. Sato, H. Enei, T. Watanabe, and M. Kuwahara. 2001. Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals. Appl Microbiol Biotechnol 56:707-9. 62. Irie, T., Y. Honda, T. Watanabe, and M. Kuwahara. 2001. Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA. Appl Microbiol Biotechnol 55:563-5. 63. Irie, T., Y. Honda, T. Watanabe, and M. Kuwahara. 2001. Homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus Pleurotus ostreatus. Appl Microbiol Biotechnol 55:566-70. 64. Irie, T., T. Sato, K. Saito, Y. Honda, T. Watanabe, M. Kuwahara, and H. Enei. 2003. Construction of a homologous selectable marker gene for Lentinula edodes transformation. Biosci Biotechnol Biochem 67:2006-2009. 65. Jain, S., H. Durand, and G. Tiraby. 1992. Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker. Mol Gen Genet 234:489-93. 66. Juge, N., B. Svensson, and G. Williamson. 1998. Secretion, purification, and characterisation of barley alpha-amylase produced by heterologous gene expression in Aspergillus niger. Appl Microbiol Biotechnol 49:385-92. 67. Kapusta, J., A. Modelska, M. Figlerowicz, T. Pniewski, M. Letellier, O. Lisowa, V. Yusibov, H. Koprowski, A. Plucienniczak, and A. B. Legocki. 1999. A plant-derived edible vaccine against hepatitis B virus. Faseb J 13:1796-9. 68. Kauffman, K. J., E. M. Pridgen, F. J. Doyle, 3rd, P. S. Dhurjati, and A. S. Robinson. 2002. Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae. Biotechnol Prog 18:942-50. 69. Kemppainen, M., A. Circosta, D. Tagu, F. Martin, and A. G. Pardo. 2005. Agrobacterium-mediated transformation of the ectomycorrhizal symbiont Laccaria bicolor S238N. Mycorrhiza 16:19-22. 70. Khashnobish, A., A. Hamann, and H. D. Osiewacz. 1999. Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina. Appl Microbiol Biotechnol 52:191-5. 71. Kilstrup, M., and K. N. Kristiansen. 2000. Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer. Nucleic Acids Res 28:E55. 72. Kim, K., Y. Leem, K. Kim, K. Kim, and H. T. Choi. 2002. Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration. FEMS Microbiol Lett 209:273-6. 73. Kim, S., J. Song, and H. T. Choi. 2004. Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration. FEMS Microbiol Lett 233:201-4. 74. Kojic, M., and W. K. Holloman. 2000. Shuttle vectors for genetic manipulations in Ustilago maydis. Can J Microbiol 46:333-8. 75. Kolar, M., P. J. Punt, C. A. van den Hondel, and H. Schwab. 1988. Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene. Gene 62:127-34. 76. Kong, Q., L. Richter, Y. F. Yang, C. J. Arntzen, H. S. Mason, and Y. Thanavala. 2001. Oral immunization with hepatitis B surface antigen expressed in transgenic plants. Proc Natl Acad Sci U S A 98:11539-44. 77. Koziel, M. G., N. B. Carozzi, and N. Desai. 1996. Optimizing expression of transgenes with an emphasis on post-transcriptional events. Plant Mol Biol 32:393-405. 78. Krecic, A. M., and M. S. Swanson. 1999. hnRNP complexes: composition, structure, and function. Curr Opin Cell Biol 11:363-71. 79. Kuo, C. Y., S. Y. Chou, and C. T. Huang. 2004. Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes. Appl Microbiol Biotechnol 65:593-9. 80. Kurashige, S., Y. Akuzawa, and F. Endo. 1997. Effects of Lentinus edodes, Grifola frondosa and Pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, N-butyl-N-butanolnitrosoamine. Immunopharmacol Immunotoxicol 19:175-83. 81. Kusnadi, A. R., Nikolov, Zivko L., Howard, John A. 1997. Production of recombinant proteins in transgenic plants: practical considerations. Biotechnology and Bioengineering 56:473-84. 82. Lacroix, B., T. Tzfira, A. Vainstein, and V. Citovsky. 2006. A case of promiscuity: Agrobacterium's endless hunt for new partners. Trends Genet 22:29-37. 83. Larraya, L. M., G. Perez, I. Iribarren, J. A. Blanco, M. Alfonso, A. G. Pisabarro, and L. Ramirez. 2001. Relationship between monokaryotic growth rate and mating type in the edible basidiomycete Pleurotus ostreatus. Appl Environ Microbiol 67:3385-90. 84. Li, B., F. A. Rotsaert, M. H. Gold, and V. Renganathan. 2000. Homologous expression of recombinant cellobiose dehydrogenase in Phanerochaete chrysosporium. Biochem Biophys Res Commun 270:141-6. 85. Li, D., H. L. Youngs, and M. H. Gold. 2001. Heterologous expression of athermostable manganese peroxidase from Dichomitus squalens in Phanerochaete chrysosporium. Arch Biochem Biophys 385:348-56. 86. Li, G., R. Li, Q. Liu, Q. Wang, M. Chen, and B. Li. 2006. A highly efficient polyethylene glycol-mediated transformation method for mushrooms. FEMS Microbiol Lett 256:203-8. 87. Lin, G., X. Cai, and R. M. Johnstone. 1997. Expression cloning of a mammalian amino acid transporter or modifier by complementation of a yeast transport mutant. J Cell Physiol 173:351-60. 88. Linnemannstons, P., T. Voss, P. Hedden, P. Gaskin, and B. Tudzynski. 1999. Deletions in the gibberellin biosynthesis gene cluster of Gibberella fujikuroi by restriction enzyme-mediated integration and conventional transformation-mediated mutagenesis. Appl Environ Microbiol 65:2558-64. 89. Lugones, L. G., K. Scholtmeijer, R. Klootwijk, and J. G. Wessels. 1999. Introns are necessary for mRNA accumulation in Schizophyllum commune. Mol Microbiol 32:681-9. 90. Ma, B., M. B. Mayfield, and M. H. Gold. 2001. The green fluorescent protein gene functions as a reporter of gene expression in Phanerochaete chrysosporium. Appl Environ Microbiol 67:948-55. 91. Ma, J. K., A. Hiatt, M. Hein, N. D. Vine, F. Wang, P. Stabila, C. van Dolleweerd, K. Mostov, and T. Lehner. 1995. Generation and assembly of secretory antibodies in plants. Science 268:716-9. 92. Mason, H. S., and C. J. Arntzen. 1995. Transgenic plants as vaccine production systems. Trends Biotechnol 13:388-92. 93. Mason, H. S., J. M. Ball, J. J. Shi, X. Jiang, M. K. Estes, and C. J. Arntzen. 1996. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci U S A 93:5335-40. 94. Mason, H. S., D. M. Lam, and C. J. Arntzen. 1992. Expression of hepatitis B surface antigen in transgenic plants. Proc Natl Acad Sci U S A 89:11745-9. 95. Mayfield, M. B., K. Kishi, M. Alic, and M. H. Gold. 1994. Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 60:4303-9. 96. McGarvey, P. B., J. Hammond, M. M. Dienelt, D. C. Hooper, Z. F. Fu, B. Dietzschold, H. Koprowski, and F. H. Michaels. 1995. Expression of the rabies virus glycoprotein in transgenic tomatoes. Biotechnology 13:1484-7. 97. Mikosch, T. S., B. Lavrijssen, A. S. Sonnenberg, and L. J. van Griensven. 2001. Transformation of the cultivated mushroom Agaricus bisporus (Lange) using T-DNA from Agrobacterium tumefaciens. Curr Genet 39:35-9. 98. Mizuno, M., M. Morimoto, K. Minato, and H. Tsuchida. 1998. Polysaccharides from Agaricus blazei stimulate lymphocyte T-cell subsets in mice. Biosci Biotechnol Biochem 62:434-7. 99. Modelska, A., B. Dietzschold, N. Sleysh, Z. F. Fu, K. Steplewski, D. C. Hooper, H. Koprowski, and V. Yusibov. 1998. Immunization against rabies with plant-derived antigen. Proc Natl Acad Sci U S A 95:2481-5. 100. Moore-Landecker, E. 1996. Fundamentals of the fungi. Prentice-Hall, Inc, USA. 101. Mumberg, D., R. Muller, and M. Funk. 1995. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156:119-22. 102. Murasugi, A., S. Tanaka, N. Komiyama, N. Iwata, K. Kino, H. Tsunoo, and S. Sakuma. 1991. Molecular cloning of a cDNA and a gene encoding an immunomodulatory protein, Ling Zhi-8, from a fungus, Ganoderma lucidum. J Biol Chem 266:2486-93. 103. Nakai, R., K. Sen, S. Kurosawa, and H. Shibai. 2000. Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes. FEMS Microbiol Lett 190:51-6. 104. Noel, T., and J. Labarere. 1994. Homologous transformation of the edible basidiomycete Agrocybe aegerita with the URA1 gene: characterization of integrative events and of rearranged free plasmids in transformants. Curr Genet 25:432-7. 105. Norris, S. R., S. E. Meyer, and J. Callis. 1993. The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression. Plant Mol Biol 21:895-906. 106. Ogawa, K., T. Yamazaki, T. Hasebe, S. Kajiwara, A. Watanabe, Y. Asada, and K. Shishido. 1998. Molecular breeding of the basidiomycete Coprinus cinereus strains with high lignin-decolorization and b-degradation activities using novel heterologous protein expression vectors. Appl Microbiol Biotechnol 49:285-9. 107. Okamura, T., T. Ogata, N. Minamimoto, T. Takeno, H. Noda, S. Fukuda, and M. Ohsugi. 2001. Characteristics of wine produced by mushroom fermentation. Biosci Biotechnol Biochem 65:1596-600. 108. Paramban, R. I., R. C. Bugos, and W. W. Su. 2004. Engineering green fluorescent protein as a dual functional tag. Biotechnol Bioeng 86:687-97. 109. Park, E. H., Y. M. Shin, Y. Y. Lim, T. H. Kwon, D. H. Kim, and M. S. Yang. 2000. Expression of glucose oxidase by using recombinant yeast. J Biotechnol 81:35-44. 110. Park, S., and W. F. Ramirez. 1990. Effect of transcription promoters on the optimal production of secreted protein in fed-batch reactors. Biotechnol Prog 6:311-8. 111. Peberdy, J. F. 1979. Fungal protoplasts: isolation, reversion, and fusion. Annu Rev Microbiol 33:21-39. 112. Peng, M. 1993. Improved conditions for protoplast formation and transformation of Pleurotus ostreatus. Appl Microbiol Biotechnol 40:101. 113. Peng, M., P. A. Lemke, and N. K. Singh. 1993. A nucleotide sequence involved in replicative transformation of a filamentous fungus. Curr Genet 24:114-21. 114. Peng, M., N. K. Singh, and P. A. Lemke. 1992. Recovery of recombinant plasmids from Pleurotus ostreatus transformants. Curr Genet 22:53-9. 115. Penna, T. C., M. Ishii, A. P. Junior, L. de Oliveira Nascimento, L. C. de Souza, and O. Cholewa. 2004. Evaluation of recombinant green fluorescent protein, under various culture conditions and purification with HiTrap hydrophobic interaction chromatography resins. Appl Biochem Biotechnol 113-116:453-68. 116. Penttila, M., H. Nevalainen, M. Ratto, E. Salminen, and J. Knowles. 1987. A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61:155-64. 117. Persidis, A. 1999. Agricultural biotechnology. Nat Biotechnol 17:612-4. 118. Peterson, D. L. 1987. The structure of hepatitis B surface antigen and its antigenic sites. Bioessays 6:258-62. 119. Piechaczyk, M., J. M. Blanchard, L. Marty, C. Dani, F. Panabieres, S. El Sabouty, P. Fort, and P. Jeanteur. 1984. Post-transcriptional regulation of glyceraldehyde-3-phosphate- dehydrogenase gene expression in rat tissues. Nucleic Acids Res 12:6951-63. 120. Pluddemann, A., and W. H. Van Zyl. 2003. Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model. Curr Genet 43:439-46. 121. Poppenborg, L., K. Friehs, and E. Flaschel. 1997. The green fluorescent protein is a versatile reporter for bioprocess monitoring. J Biotechnol 58:79-88. 122. Punt, P. J., R. P. Oliver, M. A. Dingemanse, P. H. Pouwels, and C. A. van den Hondel. 1987. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-24. 123. Punt, P. J., N. van Biezen, A. Conesa, A. Albers, J. Mangnus, and C. van den Hondel. 2002. Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol 20:200-6. 124. Richter, L. J., Y. Thanavala, C. J. Arntzen, and H. S. Mason. 2000. Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nat Biotechnol 18:1167-71. 125. Ridder, R., and H. D. Osiewacz. 1992. Sequence analysis of the gene coding for glyceraldehyde-3-phosphate dehydrogenase (GPD) of Podospora anserina: use of homologous regulatory sequences to improve transformation efficiency. Curr Genet 21:207-13. 126. Robinson, M., and A. Sharon. 1999. Transformation of the bioherbicide Colletotrichum gloeosporioides f. sp. Aeschynomene By electroporation of germinated conidia. Curr Genet 36:98-104. 127. Rozman, D., and R. Komel. 1991. Altered expression of the steroid bioconverting pathway in pAN 7-1 transformants of Cochliobolus lunatus. Curr Genet 20:385-9. 128. Rozman, D., and R. Komel. 1992. Transformation of Cochliobolus lunatus with pUT 720 changes the steroid hydroxylating ability of the fungus. Curr Genet 22:123-7. 129. Sato, T., K. Yaegashi, S. Ishii, T. Hirano, S. Kajiwara, K. Shishido, and H. Enei. 1998. Transformation of the edible basidiomycete Lentinus edodes by restriction enzyme-mediated integration of plasmid DNA. Biosci Biotechnol Biochem 62:2346-50. 130. Schiestl, R. H., and T. D. Petes. 1991. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88:7585-9. 131. Scholtmeijer, K., H. A. Wosten, J. Springer, and J. G. Wessels. 2001. Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune. Appl Environ Microbiol 67:481-3. 132. Schouten, A., J. Roosien, F. A. van Engelen, G. A. de Jong, A. W. Borst-Vrenssen, J. F. Zilverentant, D. Bosch, W. J. Stiekema, F. J. Gommers, A. Schots, and J. Bakker. 1996. The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. Plant Mol Biol 30:781-93. 133. Schuren, F. H., M. C. Harmsen, and J. G. Wessels. 1993. A homologous gene-reporter system for the basidiomycete Schizophyllum commune based on internally deleted homologous genes. Mol Gen Genet 238:91-6. 134. Schuren, F. H., and J. G. Wessels. 1998. Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts. Curr Genet 33:151-6. 135. Schuren, F. H., and J. G. Wessels. 1994. Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance. Curr Genet 26:179-83. 136. Sears, I. B., J. O'Connor, O. W. Rossanese, and B. S. Glick. 1998. A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Yeast 14:783-90. 137. Seeger, C., and W. S. Mason. 2000. Hepatitis B virus biology. Microbiol Mol Biol Rev 64:51-68. 138. Sharma, K. K., S. Gupta, and R. C. Kuhad. 2006. Agrobacterium-mediated delivery of marker genes to Phanerochaete chrysosporium mycelial pellets: a model transformation system for white-rot fungi. Biotechnol Appl Biochem 43:181-6. 139. She, Q. B., T. B. Ng, and W. K. Liu. 1998. A novel lectin with potent immunomodulatory activity isolated from both fruiting bodies and cultured mycelia of the edible mushroom Volvariella volvacea. Biochem Biophys Res Commun 247:106-11. 140. Shi, Z., D. Christian, and H. Leung. 1995. Enhanced transformation in Magnaporthe grisea by restriction enzyme mediated intergration of plasmid DNA. Phytopathology 85. 141. Sijmons, P. C., B. M. Dekker, B. Schrammeijer, T. C. Verwoerd, P. J. van den Elzen, and A. Hoekema. 1990. Production of correctly processed human serum albumin in transgenic plants. Biotechnology 8:217-21. 142. Simpson, G. G., and W. Filipowicz. 1996. Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery. Plant Mol Biol 32:1-41. 143. Sivan, A., T. E. Stasz, M. Hemmat, C. K. Hayes, and G. E. Harman. 1992. Transformation of Trichoderma spp. with plasmids conferring hygromycin B resistance. mycologia 84:687-694. 144. Sojikul, P., N. Buehner, and H. S. Mason. 2003. A plant signal peptide-hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells. Proc Natl Acad Sci U S A 100:2209-14. 145. Sollewijn Gelpke, M. D., M. Mayfield-Gambill, G. P. Lin Cereghino, and M. H. Gold. 1999. Homologous expression of recombinant lignin peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 65:1670-4. 146. Streatfield, S. J. 2005. Oral hepatitis B vaccine candidates produced and delivered in plant material. Immunol Cell Biol 83:257-62. 147. Su, W. W. 2005. Fluorescent proteins as tools to aid protein production. Microb Cell Fact 4:12. 148. Sun, L., H. Cai, W. Xu, Y. Hu, and Z. Lin. 2002. CaMV 35S promoter directs beta-glucuronidase expression in Ganoderma lucidum and Pleurotus citrinopileatus. Mol Biotechnol 20:239-44. 149. Sun, L., W. Xu, Y. Hu, Y. Gao, and Z. Lin. 2001. Efficient transformation of the medicinal mushroom Ganoderma lucidum. Plant Molecular Biology Reporter 19:383-393. 150. Sunagawa, M., and Y. Magae. 2002. Transformation of the edible mushroom Pleurotus ostreatus by particle bombardment. FEMS Microbiol Lett 211:143-6. 151. Tacket, C. O., H. S. Mason, G. Losonsky, J. D. Clements, M. M. Levine, and C. J. Arntzen. 1998. Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nat Med 4:607-9. 152. Takeda, S., E. Tamaki, K. Miura, and I. Kumagai. 1990. Expression and secretion of goat alpha-lactalbumin as an active protein in Saccharomyces cerevisiae. Biochem Biophys Res Commun 173:741-7. 153. Tanaka, A., S. Mita, S. Ohta, J. Kyozuka, K. Shimamoto, and K. Nakamura. 1990. Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron. Nucleic Acids Res 18:6767-70. 154. Tanudji, M., S. Hevi, and S. L. Chuck. 2002. Improperly folded green fluorescent protein is secreted via a non-classical pathway. J Cell Sci 115:3849-57. 155. Thanavala, Y., M. Mahoney, S. Pal, A. Scott, L. Richter, N. Natarajan, P. Goodwin, C. J. Arntzen, and H. S. Mason. 2005. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci U S A 102:3378-82. 156. Thanavala, Y., Y. F. Yang, P. Lyons, H. S. Mason, and C. Arntzen. 1995. Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc Natl Acad Sci U S A 92:3358-61. 157. Thrane, C., M. Lubeck, H. Green, Y. Degefe, S. Allerup, U. Thrane, and D. Funck Jensen. 1995. A tool for monitoring Trichoderma harzianum: transformation with the GUS gene by protoplast technology. Phytopathology 85:1428-1435. 158. Tiollais, P., C. Pourcel, and A. Dejean. 1985. The hepatitis B virus. Nature 317:489-95. 159. Tsukihara, T., Y. Honda, T. Watanabe, and T. Watanabe. 2006. Molecular breeding of white rot fungus Pleurotus ostreatus by homologous expression of its versatile peroxidase MnP2. Appl Microbiol Biotechnol 71:114-20. 160. van Berkel, P. H., M. M. Welling, M. Geerts, H. A. van Veen, B. Ravensbergen, M. Salaheddine, E. K. Pauwels, F. Pieper, J. H. Nuijens, and P. H. Nibbering. 2002. Large scale production of recombinant human lactoferrin in the milk of transgenic cows. Nat Biotechnol 20:484-7. 161. van de Rhee, M. D., P. M. Graca, H. J. Huizing, and H. Mooibroek. 1996. Transformation of the cultivated mushroom, Agaricus bisporus, to hygromycin B resistance. Mol Gen Genet 250:252-8. 162. van de Rhee, M. D., O. Mendes, M. W. Werten, H. J. Huizing, and H. Mooibroek. 1996. Highly efficient homologous integration via tandem exo-beta-1, 3-glucanase genes in the common mushroom, Agaricus bisporus. Curr Genet 30:166-73. 163. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27059 | - |
| dc.description.abstract | 本研究成功建立了簡便且具有通用性之食用菇轉形策略，以發芽的擔孢子或是小片段的營養菌絲為材料，利用電穿孔的方式完成轉形。此方法免除原生質體的製備及物種的限制，完成測試並成功表現重組蛋白質之菌株包含金針菇、洋菇、鮑魚菇及香菇，轉形效率約為每微克質體可得5~50株轉形株。南方氏雜合分析結果顯示異源基因隨機插入染色體中，在無選殖壓力的環境下繼代培養數次仍維持穩定。以本研究所建構之pMush系列表現載體，可放置受測異源基因於載體中的多限制酶切位(multiple cloning site, MCS)以進行重組蛋白質表現測試。使用gpd啟動子及其第一個intron，可使金針菇、洋菇及鮑魚菇成功表現Egfp，表現量最高分別可占總可溶性蛋白質的2%、1%及0.5%。Egfp可在金針菇生活史中的每個階段表現，包括基原體、成熟之子實體、經減數分裂所生成之擔孢子及同核體。雙核菌株及其減數分裂所生成同核體之南方氏雜合分析訊號相符，顯示外來基因在減數分裂後仍能穩定存在。使用同源gpd啟動子及其第一個intron，香菇之GUS表現量最高可占總可溶性蛋白質的0.05%。以B型肝炎表面抗原為目標測試菇菌生產口服疫苗之可能性，發現野生型B型肝炎表面抗原基因序列遭受錯誤的剪輯，且有多個菇菌不常用之密碼子。經過序列修飾之B型肝炎表面抗原基因，可在香菇中順利生成重組蛋白質，於蛋白質C端外加內質網停留訊息胜肽HDEL，可使B型肝炎表面抗原表現量增加三倍，表現量占總可溶性蛋白質 1.31 | zh_TW |
| dc.description.abstract | A simple and applicable mushroom transformation procedure based on electroporation of basidiospores or mycelial fragments was developed. This method eliminated the problem of protoplast preparation and the limitation of host specificity. Flammulina velutipes, Agaricus bisporus, Pleurotus ostreatus, and Lentinula edodes were transformed and expressed recombinant protein successfully; the transformation efficiency were 5~50 transformants per ug DNA. Southern analysis of transformants indicated that the integration of heterologous genes might occur randomly and were maintained stably after multiple rounds of subculture without selection pressure. Heterologous genes tested for recombinant proteins expression were cloned in the multiple cloning site of pMush-series expression vectors constructed in this study. Fused to the glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter with its first intron, Egfp could be expressed successfully in F. velutipes, A. bisporus and P. ostreatus, and the highest expression of EGFP was 2%, 1%, and 0.5% of total soluble protein, respectively. Egfp was expressed during the whole life cycle of F. velutipes including primordia, mature fruiting bodies, basidiospores and monokaryons that produced from meiosis. Southern analysis of dikaryon and its green progeny showed all signals of progeny coincide to those of parental dikaryon. These results revealed that the integrated DNA was stable during meiosis. In the case of L. edodes, the highest expression of GUS was 0.05% of total soluble protein by using homologous gpd promoter and the 1st intron. Incorrect splicing and several uncommon codons were found when wild type Hepatitis B surface antigen gene was tested as oral vaccine produced in mushrooms. Modified Hepatitis B surface antigen gene could be expressed in L. edodes, and an ER retention signal (HDEL) fused to its C terminus can enhance the expression by threefold, as 1.31 ug/g of total soluble protein. The formation of hepatitis B virus-like particles with a diameter of 20 nm was revealed by transmission electron microscopy and the production of specific antibody was induced by oral immunization or injection in animal trials. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-12T17:54:39Z (GMT). No. of bitstreams: 1 ntu-97-D91623803-1.pdf: 2886206 bytes, checksum: a35c15210dd4f86e8646a8be7d72a353 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 摘 要 I
ABSTRACT II 圖 目 錄 VI 表 目 錄 VII 壹、總論 1 一、基因轉形 1 二、分子農場 6 1. 生產特用產物 6 2. 開發食用疫苗 8 3. 轉基因菇類於分子農場的應用 12 三、菇類與分子生物技術 16 1. 菇類概述 16 2. 菇類分子育種與分子農場 17 四、研究動機 19 貳、菇菌基因轉形系統之建立與報導者基因之表現 22 一、前言 22 1. 宿主(host)細胞模式 22 2. 載體 25 2.1 酵母菌表達系統 25 2.2 絲狀真菌表達系統 27 3. 轉形策略 28 3.1 PEG法 29 3.2 REMI (restriction enzyme mediated integration)法 29 3.3 電穿孔法 29 3.4 土壤農桿菌法 30 二、材料與方法 32 1. 菌株與培養 32 2. 鮑魚菇gpd cloning 33 2.1 鮑魚菇部分甘油醛-3-磷酸脫氫酶選殖 33 2.2 鮑魚菇甘油醛-3-磷酸脫氫酶擴增選殖 33 3. 質體與建構 34 3.1 商用載體 34 3.2 轉形測試質體建構 36 3.3 pMush系列載體建構 37 3.4 GUS表現質體之建立 44 3.5 EGFP表現質體之建立 44 3.6 建構具有第一個gpd intron 的報導者表現載體 44 4. 轉形條件測試 47 5. 轉形材料改進 47 6. 轉形株鑑定 47 6.1 PCR 47 6.2 Southern hybridization 47 7. 報導者基因表現檢測 48 7.1 GUS 活性定性測試 48 7.2 GUS 活性定量測試 48 7.3 GUS 比活性測定 49 7.4 EGFP表現之螢光顯微鏡檢定與ELISA定量 49 7.5 EGFP西方雜合分析 49 8. 轉形株穩定性檢測及出菇測試 50 三、結果 51 1. 食用菇轉形系統測試、建立與報導者基因之表現 51 1.1 金針菇 56 1.2 香菇 63 1.3 鮑魚菇 67 1.4 洋菇 70 四、討論 72 参、B型肝炎表面抗原基因於食用菇之表現研究 82 一、前言 82 二、材料與方法 86 1. 菌株與培養 86 2. HBsAg序列修正與基因合成 86 3. 質體與建構 88 4. 菌株轉形與轉形株挑選 92 5. RT-PCR檢測 92 6. HBsAg檢測 92 7. 以超高速離心分離HBsAg類病毒顆粒(virus like particle, VLP) 93 8. 以穿透式電子顯微鏡進行HBsAg VLP觀測 93 9. 以小鼠模式進行HBsAg專一性抗體生成測試 93 三、結果 94 1. HBsAg基因於金針菇與香菇之表現測試 94 2. 以質體改進與蛋白質融合策略測試HBsAg之表現 94 3. 金針菇轉形株Egfp-HBsAg之RT-PCR檢測 96 4. HBsAg基因序列修正與合成 99 5. mHBsAg基因於食用菇之表現 99 5.1 mHBsAg 在香菇之表現 101 5.2 mHBsAg-HDEL在香菇之表現 102 5.3 Intron-mHBsAg在香菇之表現 103 5.4 Intron-mHBsAg-HDEL在香菇之表現 103 6. 以超高速離心分離HBsAg VLP 105 7. 以穿透式電子顯微鏡進行HBsAg VLP觀測 106 8. 以小鼠模式進行HBsAg專一性抗體生成測試 108 四、討論 110 肆、綜合討論 115 一、轉形系統 115 二、蛋白質表現限制因子 116 三、表現量分析 119 四、GMO管理 120 伍、總結 122 陸、未來工作與展望 123 參考文獻 124 | |
| dc.language.iso | zh-TW | |
| dc.subject | gpd啟動子 | zh_TW |
| dc.subject | 口服疫苗 | zh_TW |
| dc.subject | B型肝炎表面抗原 | zh_TW |
| dc.subject | 綠色螢光蛋白質 | zh_TW |
| dc.subject | 電穿孔 | zh_TW |
| dc.subject | 食用菇 | zh_TW |
| dc.subject | 基因轉形 | zh_TW |
| dc.subject | 異源基因表現 | zh_TW |
| dc.subject | heterologous expression | en |
| dc.subject | transformation | en |
| dc.subject | oral vaccine | en |
| dc.subject | mushroom | en |
| dc.subject | electroporation | en |
| dc.subject | gpd promoter | en |
| dc.subject | EGFP | en |
| dc.subject | Hepatitis B surface antigen | en |
| dc.title | 食用菇異源基因表現系統之建立及其應用 | zh_TW |
| dc.title | Development and Application of Mushroom Heterologous Gene Expression Systems | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 許瑞祥,莊榮輝,常怡雍,謝兆樞 | |
| dc.subject.keyword | gpd啟動子,異源基因表現,基因轉形,食用菇,電穿孔,綠色螢光蛋白質,B型肝炎表面抗原,口服疫苗, | zh_TW |
| dc.subject.keyword | gpd promoter,heterologous expression,transformation,mushroom,electroporation,EGFP,Hepatitis B surface antigen,oral vaccine, | en |
| dc.relation.page | 138 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-02-04 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-97-1.pdf 未授權公開取用 | 2.82 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。