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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 蘇霩靄 | |
dc.contributor.author | Chia-Wei Ro | en |
dc.contributor.author | 羅嘉瑋 | zh_TW |
dc.date.accessioned | 2021-06-12T17:54:09Z | - |
dc.date.available | 2016-10-07 | |
dc.date.copyright | 2011-10-07 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-09 | |
dc.identifier.citation | 1. Baer JG, Miranda H, Fernandez W, and Medina J. Human diphyllobothriasis in Peru. Z Parasitenkd. 1967; 28(3): 277-89.
2. Bhadury P, Austen MC, Bilton DT, Lambshead PJD, Rogers AD, and Smerdon GR. Exploitation of archived marine nematodes-a hot lysis DNA extraction protocol for molecular studies. Zool Scr. 2007; 36(1): 93-8. 3. Carlton JM, Hirt RP, Silva JC, Delcher AL, Schatz M, Zhao Q, Wortman JR, Bidwell SL, Alsmark UC, Besteiro S, Sicheritz-Ponten T, Noel CJ, Dacks JB, Foster PG, Simillion C, Van de Peer Y, Miranda-Saavedra D, Barton GJ, Westrop GD, Müller S, Dessi D, Fiori PL, Ren Q, Paulsen I, Zhang H, Bastida-Corcuera FD, Simoes-Barbosa A, Brown MT, Hayes RD, Mukherjee M, Okumura CY, Schneider R, Smith AJ, Vanacova S, Villalvazo M, Haas BJ, Pertea M, Feldblyum TV, Utterback TR, Shu CL, Osoegawa K, de Jong PJ, Hrdy I, Horvathova L, Zubacova Z, Dolezal P, Malik SB, Logsdon JM Jr, Henze K, Gupta A, Wang CC, Dunne RL, Upcroft JA, Upcroft P, White O, Salzberg SL, Tang P, Chiu CH, Lee YS, Embley TM, Coombs GH, Mottram JC, Tachezy J, Fraser-Liggett CM, and Johnson PJ. Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis. Science. 2007; 315(5809): 207-12. 4. Chamberlain JS, Gibbs RA, Ranier JE, Nguyen PN, and Caskey CT. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 1988; 16(23): 11141-56. 5. Chervy L. The terminology of larval cestodes or metacestodes. Syst Parasitol. 2002; 52: 1-33. 6. Chou HF, Yen CM, Liang WC, and Jong YJ. Diphyllobothriasis latum: the first child case report in Taiwan. Kaohsiung J Med Sci. 2006; 22(7): 346-51. 7. deVos T and Dick TA. Differentiation between Diphyllobothrium dendriticum and D. latum using isozymes, restriction profiles and ribosomal gene probes. Syst Parasitol. 1989; 13: 161-6. 8. Diamond LS. The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol. 1957; 43(4): 488-90. 9. Eom KS and Rim HJ. Morphologic descriptions of Taenia asiatica sp. n. Korean J Parasitol. 1993; 31(1): 1-6. 10. Fan PC and Chung WC. Sociocultural factors and local customs related to taeniasis in east Asia. Kaohsiung J Med Sci. 1997; 13(11): 647-52. 11. Frick LP, Moon AP, and Lin SS. Parasitologic studies in the Far East. XV. A preliminary survey for parasitism in Southern Formosa. Metabolism. 1956; 5(3): 302-8. 12. Fukumoto S, Yazaki S, Kamo H, Yamane Y, and Tsuji M. Distinction between Diphyllobothrium nihonkaiense and Diphyllobothrium latum by immunoelectrophoresis. Jpn J Parasitol. 1988; 37: 91-5. 13. Grove DI. 1990. A history of human helminthology. C.A.B International. 14. Hamelmann E, Beyer K, Gruber C, Lau S, Matricardi PM, Nickel R, Niggemann B, and Wahn U. Primary prevention of allergy: avoiding risk or providing protection? Clin Exp Allergy. 2008; 38(2): 233-45. 15. Hersh SM. Pulmonary trichomoniasis and Trichomonas tenax. J Med Microbiol. 1985; 20: 1-10. 16. Honigberg BM. 1990. Trichomonads parasitic in humans. Springer-Verlag New York Inc. 17. Ito A, Wandra T, Yamasaki H, Nakao M, Sako Y, Nakaya K, Margono SS, Suroso T, Gauci C, and Lightowlers MW. Review: cysticercosis/taeniasis in Asia and the Pacific. Vector Borne Zoonotic Dis. 2004; 4(2): 95-107. 18. Jeon HK, Chai JY, Kong Y, Waikagul J, Insisiengmay B, Rim HJ, and Eom KS. Differential diagnosis of Taenia asiatica using multiplex PCR. Exp parasitol. 2009a; 121: 151-6. 19. Jeon HK, Kim KH, Huh S, Chai JY, Min DY, Rim HJ, and Eom KS. Morphologic and genetic identification of Diphyllobothrium nihonkaiense in Korea. Korean J Parasitol. 2009b; 47(4): 369-75. 20. John F and Bergner JR. Intestinal parasites in an aborigine village in Southeast Taiwan. Am J Trop Med Hyg. 1964; 13: 78-81. 21. Jongwutiwes S, Silachamroon U, and Putaporntip C. Pentatrichomonas hominis in empyema thoracis. Trans R Soc Trop Med Hyg. 2000; 94: 185-6. 22. Jozefzoon LM, and Oostburg BF. Detection of hookworm and hookworm-like larvae in human fecocultures in Suriname. Am J Trop Med Hyg. 1994; 51(4): 501-5. 23. Kikuta N, Yamamoto A, Fukura K, and Goto N. Specific and sensitive detection of Trichomonas tenax by the polymerase chain reaction. Lett Appl Microbiol. 1997; 24: 193-7. 24. Lai PF, Yiang GT, Chang H, Wu RC, Hu SC, and Chen KC. Cerevral sparganosis. Tzu Chi Med J. 2010; 22(2): 115-8. 25. Lee SH, Chai JY, Seo BS, and Cho SY. Two cases of human infection by adult of Spirometra erinacei. Korean J Parasitol. 1984; 22(1): 66-71. 26. Lee SU, Huh S, and Phares CK. Genetic comparison between Spirometra erinacei and S. mansonoides using PCR-RFLP analysis. Korean J Parasitol. 1997; 35(4): 277-82. 27. Li J, Liao X, and Yang H. Molecular characterization of a parasitic tapeworm (Ligula) based on DNA sequences from formalin-fixed specimens. Biochem Genet. 2000; 38(9-10): 309-22. 28. Lin CK, Liao CH, Su KE, and Lee TH. Colonoscopic diagnosis of ancylostoma ceylanicum infection: report of a case. Gastroenterol J Taiwan. 2009; 26(1): 21-6. 29. Lou HY, Tsai PC, Chang CC, Lin YH, Liao CW, Kao TC, Lin HC, Lee WC, and Fan CK. A case of human diphyllobothriasis in northern Taiwan after eating raw fish fillets. J Microbiol Immunol Infect. 2007; 40: 452-6. 30. Mallat H, Podglajen I, Lavarde V, Mainardi JL, Frappier J, and Cornet M. Molecular characterization of Trichomonas tenax causing pulmonary infection. J Clin Microbiol. 2004; 42(8): 3886-7. 31. Markell EK. Pseudohookworm infection-trichostrongyliasis. Treatment with thiabendazole. Engl J Med. 1968; 278(15): 831-2. 32. Matsuura T, Bylund G, and Sugane K. Comparison of restriction fragment length polymorphisms of ribosomal DNA between Diphyllobothrium nihonkaiense and D. latum. J Helminthol. 1992; 66: 261-6. 33. Miyazaki I. Helminthic Zoonoses. International Medical Foundation of Japan. 1991. 34. Mortimer K, Brown A, Feary J, Jagger C, Lewis S, Antoniak M, Pritchard D, and Britton J. Does-ranging study for trials of therapeutic infection with Necator americanus in humans. Am J Trop Med Hyg. 2006; 75(5): 914-20. 35. Nakao M, Abmed D, Yamasaki H, and Ito A. Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda: Diphyllobothriidae). Parasitol Res. 2007; 101: 233-6. 36. Park JK, Kim KH, Kang S, Jeon HK, Kim JH, Littlewood DT, and Eom KS. Characterization of the mitochondrial genome of Diphyllobothrium latum (Cestoda: Pseudophyllidea) – implications for the phylogeny of eucestodes. Parasitology. 2007; 134: 749-59. 37. Rohela M, Jamaiah I, Chan KW, and Wan Yusoff WS. Diphyllobothriasis: the first case report from Malaysia. Southeast Asian J Trop Med Public Health. 2002; 33(2): 229-30. 38. Sato M, Sanguankiat S, Yoonuan T, Pongvongsa T, Keomoungkhoun M, Phimmayoi I, Boupa B, Moji K, and Waikagul J. Copro-molecular identification of infections with hookworm eggs in rural Lao PDR. Trans R Soc Trop Med Hyg. 2010; 104(9): 617-22. 39. Scholz T, Garcia HH, Kuchta R, and Wicht B. Update on the human broad tapeworm (genus Diphyllobothrium), including clinical relevance. Clin Microbiol Rev. 2009; 22(1): 146-60. 40. Schwebke JR and Burgess D. Trichomoniasis. Clin Microbiol Rev. 2004; 17(4): 794-803. 41. Simanjuntak GM, Margono SS, Okamoto M, Ito A. Taeniasis/cysticercosis in Indonesia as an emerging disease. Parasitol Today. 1997; 13: 321-3. 42. Suzuki N and Kumazawa H. A case of human infection with the adult of Spirometra erinacei (Rudolphi, 1819) Faust, Campbell and Kellogg, 1929. Jpn J Parasitol. 1982; 31(1): 23-6. 43. Tanowitz HB, Weiss LM, and Wittner M. Tapeworms. Curr Infect Dis Rep. 2001; 3: 77-84. 44. Torres P, Villalobos L, and Woelfl S. Experimental infection of copepods from four lakes in southern Chile with Diphyllobothrium latum (Linnaeus, 1758) coracidia. Comp Parasitol. 2007; 74(1): 167-70. 45. Tu CH, Liao WC, Chiang TH, and Wang HP. Pet parasites infesting the human colon. Gastrointest Endosc. 2008; 67(1): 159-60. 46. von Bonsdorff B. Pernicious anemia caused by Diphyllobothrium latum, in the light of recent investigation. Blood. 1948; 3: 91-102. 47. Walton BC and Bacharach T. Occurrence of trichomonads in the respiratory tract. Report of three cases. J Parasitol. 1963; 49: 35-8. 48. Wantland WW and Wantland EM. Incidence, ecology, and reproduction of oral protozoa. J Dent Res. 1960; 39: 863. 49. Wicht B, Scholz T, and Kuchta R. First record of human infection with the tapeworm Diphyllobothrium nihonkaiense in North America. Am J Trop Med Hyg. 2008a; 78(2): 235-8. 50. Wicht B, deMarval F, Gottstein B, and Peduzzi R. Imported diphyllobothriasis in Switzerland: molecular evidence of Diphyllobothrium dendriticum (Nitsch, 1824). Parasitol Res. 2008b; 102: 201-4. 51. Wicht B, Yanagida T, Scholz T, Ito A, Jimenez JA, and Brabec J. Multiplex PCR for differential identification of broad tapeworms (Cestoda: Diphyllobothrium) infecting humans. J Clin Microbiol. 2010; 48(9): 3111-6. 52. Wilson MS, Taylor MD, Balic A, Finney CA, Lamb JR, and Maizels RM. Suppression of allergic airway inflammation by helminth-induced regulatory T cells. J Exp Med. 2005; 202(9): 1199-212. 53. Yamasaki H, Allan JC, Sato MO, Nakao M, Sako Y, Nakaya K, Qiu D, Mamuti W, Craig PS, and Ito A. DNA differential diagnosis of Taeniasis and cysticercosis by multiplex PCR. J Clin Microbiol. 2004; 42(2): 548-53. 54. Yamasaki H, Nakaya K, Nakao M, Sako Y, and Ito A. Significance of molecular diagnosis using histopathological specimens in cestode zoonoses. Trop Med Health. 2007; 35(4): 307-21. 55. Yeh SJ and Wu RM. Neurocysticercosis presenting with epilepsia partialis continua: a clinicopathologic report and literature review. J Formos Med Assoc. 2008; 107(7): 576-81. 56. Yera H, Nicoulaud J, and Dupouy-Camet J. Use of nuclear and mitochondrial DNA PCR and sequencing for molecular identification of Diphyllobothrium isolates potentially infective for humans. Parasite. 2008; 15: 402-7. 57. Yoshida M, Hasegawa H, Takaoka H, and Miyata A. A case of Diphyllobothrium nihonkaiense infection successfully treated by oral administration of gastrografin. Parasitol Int. 1999; 48(2): 151-5. 58. Ziem JB, Olsen A, Magnuseen P, Horton J, Agongo E, Geskus RB, and Polderman AM. Distribution and clustering of Oesophagostomum bifurcum and hookworm infections in northern Ghana. Parasitology. 2006; 132(4): 525-34. 59. Zubacova Z, Cimburek Z, and Tachezy J. Comparative analysis of trichomonad genome sizes and karyotypes. Mol Biochem Parasitol. 2008; 161: 49-54. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27030 | - |
dc.description.abstract | 臺灣的衛生條件進步之後,寄生蟲病例已較少見,不過還是有零星病例發生,外勞的飲食習慣、進口食材及國際旅遊頻繁等都是可能產生這些零星病例的原因之一。近幾年來本實驗室收到魚肉絛蟲(Diphyllobothrium)節片五例、貓糞便中Spirometra之蟲卵及Taenia節片各一例、石臘包埋之鉤蟲(hookworm)及口腔滴蟲(Trichomonas)培養液各兩例。由於種間形態特徵太接近而容易誤判、僅蟲卵無成蟲期或檢體重要部位殘缺無法鑑定等因素,我們決定利用分子生物學技術來鑑定所得檢體明確的蟲種。鑑定方法主要有蟲種專一性聚合酶連鎖反應(species-specific PCR)、multiplex PCR、序列排比(sequence alignment)及演化樹分析(phylogenetic analysis),利用的基因有核糖體核糖核酸(rRNA)、傳送核糖核酸(tRNA)、cytochrome c oxidase(cox)及NADH dehydrogenase(ND)這些在細胞內重複數多的基因作為標的或參考基因。五例魚肉絛蟲中確認一例為廣節裂頭絛蟲(Diphyllobothrium latum)、兩例為日本海裂頭絛蟲(D. nihokaiense);貓糞中Spirometra蟲卵為猬迭宮絛蟲(Spirometra erinacei),又名曼氏裂頭絛蟲(S. mansoni);一例Taenia為亞洲絛蟲(Taenia asiatica);兩例鉤蟲均為錫蘭鉤蟲(Ancylostoma ceylanicum);口腔癌病人口腔之primary cell culture裡之Trichomonas為口腔毛滴蟲(Trichomonas tenax)。自兩件經福馬林固定甚至已用卡紅染色封片之魚肉絛蟲樣本萃取之DNA,經PCR反應無法偵測到產物,可能原因是樣本受福馬林影響導致DNA萃取效率降低以及DNA已片段化(fragmentation)。故欲利用分生技術鑑定蟲種仍以文獻建議之冷凍或酒精保存者為佳。 | zh_TW |
dc.description.abstract | With the advancement in public hygiene, reports on cases of parasitic disease become less frequently seen in Taiwan. Nonetheless, sporadic cases still emerge from time to time. Immigrants’ dietary habits, imported food items as well as frequent international traveling may be the reasons why parasitic diseases still occur. We came across with five specimens of the fish tapeworm, one specimen each of Spirometra eggs in stool and Taenia segment, and two specimens each of hookworm embedded in paraffin block and primary tissue culture of oral trichomonad in recent years. Due to factors such as interspecies overlaping in morphologic features, only parasite eggs without adult stage is available and only portion of the parasite without morphologic characteristics for species identification, we decided to utilize molecular techniques for species identification. Methods employed included species-specific PCR, multiplex PCR, sequence alignment and phylogenetic analysis. Ribosomal RNA (rRNA), transfer RNA (tRNA), cytochrome c oxidase (cox) and NADH dehydrogenase (ND) genes that are highly redundant in copy numbers in a single cell were used as target or reference gene. Among the five fish tapeworm specimens, we identified one as Diphyllobothrium latum and two as D. nihonkaiense. We also identified the Spirometra eggs as S. erinacei, the Taenia segment as T. asiatica, the two paraffin embedded hookworms as Ancylostoma ceylanicum and the oral trichomonads as Trichomonas tenax. The remaining two fish tapeworm specimens that came fixed in formalin or even processed to become carmine stained whole mount specimen failed to yield detectable PCR product after DNA extraction. Formalin fixation may have lowered the efficiency of DNA extraction or caused DNA fragmentation. Thus it is better to use frozen or ethanol fixed specimens for molecular identification as suggested in the literatures. | en |
dc.description.provenance | Made available in DSpace on 2021-06-12T17:54:09Z (GMT). No. of bitstreams: 1 ntu-100-R98445202-1.pdf: 3798420 bytes, checksum: 1c7cdf02babd61be3283b3bab3222a42 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 目 錄
口試委員會審定書…………………………………………………………………...i 誌謝…………………………………………………………………………………...ii 中文摘要………………………………………………………………………….…iii 英文摘要…………………………………………………………………………. …iv 前言…………………………………………………………………………………...1 材料與方法………………………………………………………………………. ….9 結果……………………………………………………………………………….16 討論………………………………………………………………………………... .19 參考文獻…………………………………………………………………….……22 圖…………………………………………………………………………………….27 表…………………………………………………………………………………….42 | |
dc.language.iso | zh-TW | |
dc.title | 寄生蟲種類之分子鑑定 | zh_TW |
dc.title | Molecular identification of parasite species | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蕭信宏,杜文圓 | |
dc.subject.keyword | 寄生蟲,分生鑑定,聚合酶,連鎖反應,絛蟲,鉤蟲,毛滴蟲, | zh_TW |
dc.subject.keyword | parasites,molecular identification,PCR,tapeworms,hookworms,trichomonads, | en |
dc.relation.page | 48 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-09 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
Appears in Collections: | 微生物學科所 |
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