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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 潘子明 | |
dc.contributor.author | Chien-Te Lu | en |
dc.contributor.author | 呂建德 | zh_TW |
dc.date.accessioned | 2021-06-12T17:53:55Z | - |
dc.date.available | 2008-02-19 | |
dc.date.copyright | 2008-02-19 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-02-12 | |
dc.identifier.citation | 王澄清及吳哲文 (2000) 應用昆蟲學。國立台灣大學昆蟲學系。台北,台灣。401頁。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27017 | - |
dc.description.abstract | 自從基因改造作物開始商業化種植,其發展趨勢即逐年快速增長。基於食品安全上的考量,欲上市之基因改造作物必須經過嚴格的食用安全性評估,並利用準確的檢驗方法配合各國法定規範加以管理、標示。基因改造芥藍為臺灣大學葉開溫教授研究室開發之具抗蟲特性的芥藍,其轉殖蛋白 sporamin 可抑制胰蛋白酶活性而影響昆蟲消化。本研究以探討轉殖基因來源、胺基酸序列比對、模擬消化液實驗評估其是否具致過敏性;並與非基因改造芥藍比較營養成分含量,評估其是否實質等同 (substantial equivalence)。本研究利用網路資料庫搜尋基因改造芥藍轉殖蛋白 sporamin 之來源甘藷以及芥藍本身皆無相關致過敏紀錄;而 sporamin 之胺基酸序列比對結果只有過敏原 Sola t 4 與 Gly m TI 與其有較高之相似性;而模擬消化液實驗則顯示 sporamin 蛋白熱不穩定與易被消化的性質,不符合一般過敏原之特性。本研究對基因改造芥藍與非基因改造芥藍之主要營養成分包括水分、灰份、粗蛋白質、粗脂肪、膳食纖維、碳水化合物、維生素 A、維生素 B6、維生素 B12 與維生素 C 進行含量比較,結果皆無顯著差異。定性檢測系統以 sporamin 基因為目標設計引子對 spo137F/spo137R,對基因改造芥藍 DNA 樣品之靈敏度可達 0.5 ng。基因改造芥藍之定量系統建立,以 TaqManTM 與 SYBR Green I 系統所建立之定量方法,在本研究中同樣對於基因改造芥藍定量有良好的表現,其檢測範圍為 0.5% 至 100% 之基因改造芥藍,符合歐盟與我國對基因改造食品標示標準之要求。 | zh_TW |
dc.description.abstract | The development of genetically modified (GM) crops increases year by year since the commercial genetically modified crops beginning planted. However, for the concern of human health, safety assessment of GM food and development of detection system is necessary before it enter the market. Laboratory of professor Yeh had developed the insect-resistant kale by expressing the sporamin gene. In this study, we check the safety of the GM kale by search for allergic reaction reports and compare the amino acid sequence with allergen in database. The result showed that the sporamin is similar to Sola t 4 and Gly m TI by sequence alignment. But the heat-unstable and easy-digest character of sporamin is not alike allergen. For nutrient composition analysis, GM kale and wild type kale have not shown the difference on content of water, ash, protein, fat, dietary fiber, carbohydrate, vitamin A, vitamin B6, vitamin B12 and vitamin C. We use the primer pair spo137F/spo137R for qualitative analysis. The detection limit of qualitative system is 0.5 ng DNA for GM kale. Two quantitative methods, TaqManTM and SYBR Green I, based on real-time quantitative PCR were used for GM kale. Quantification range was from 0.5% to 100%. The results showed good quantitative performance in these two methods. | en |
dc.description.provenance | Made available in DSpace on 2021-06-12T17:53:55Z (GMT). No. of bitstreams: 1 ntu-97-R94b47108-1.pdf: 759070 bytes, checksum: e59b6d66be3d0d460739a784cf9f6230 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 目錄..I
圖次..IV 表次..V 縮寫表..VI 中文摘要..VII 英文摘要..VIII 第一章 前言..1 第二章 文獻回顧..4 2.1 基因改造作物..4 2.1.1 基因改造作物發展現況..4 2.1.2 基因改造作物之優點..8 2.1.3 基因改造作物潛在問題..10 2.1.4 基因改造生物相關規範..13 2.2 抗蟲蛋白..14 2.2.1 蛋白酶抑制因子..14 2.2.2 Sporamin..14 2.3 芥藍..16 2.3.1 芥藍..16 2.3.2 基因轉殖芥藍..16 2.4 基因改造食品之安全性評估..18 2.4.1 實質等同 (substantial equivalence)..18 2.4.2 過敏性反應評估 (Allergenic assessment)..18 2.4.3 基因來源與序列比對..21 2.4.4 血清學試驗..21 2.4.5 模擬腸胃液消化..22 2.4.6 動物模型試驗..22 2.4.7 皮膚針刺試驗 (skin prick test) 和雙盲法食物誘發試驗 (double-blind placebo-controlled oral-challenge test)..23 第三章 材料與方法..24 3.1 研究材料..24 3.2 藥品與試劑..24 3.3 實驗儀器與設備..26 3.4 實驗方法..27 3.4.1 探討基因來源..27 3.4.2 基因改造芥藍中抗蟲蛋白與過敏原序列之比對..27 3.4.3 模擬消化液試驗..29 3.4.4 基因改造芥藍之營養成分分析..34 3.4.5 基因改造芥藍之檢驗方法之建立..37 第四章 結果與討論..39 4.1 轉殖基因來源之食用安全性..39 4.2 Sporamin 胺基酸序列之過敏原性資料分析..40 4.3 模擬消化液試驗..44 4.4 基因改造芥藍之營養成分分析..46 4.5 基因改造芥藍之定性分析系統建立..48 4.6 基因改造芥藍之定量分析系統建立..51 4.7 標準參考物質..55 4.8 標準曲線與 PCR 放大效率..55 4.9 定量檢測系統之正確性 (accuracy) ..66 第五章 結論..75 參考文獻..76 附錄..82 附錄一 sporamin 之胺基酸序列..82 | |
dc.language.iso | zh-TW | |
dc.title | 基因改造芥藍之安全性評估及檢定 | zh_TW |
dc.title | Safety Assessment and the Detection for Genetically Modified Brassica oleracea var. alboglabra | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇遠志,方繼,葉開溫,李昆達 | |
dc.subject.keyword | 安全性評估,基因改造芥藍,sporamin, | zh_TW |
dc.subject.keyword | safety assessment,genetically modified kale,sporamin, | en |
dc.relation.page | 82 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-02-13 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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