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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26921完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 羅竹芳 | |
| dc.contributor.author | Kun-Chin Ho | en |
| dc.contributor.author | 何昆瑾 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:32:28Z | - |
| dc.date.copyright | 2008-07-02 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-06-20 | |
| dc.identifier.citation | Aderem A, Ulevitch RJ (2000) Toll-like receptors in the induction of the innate immune response. Nature 406:782-787
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Protein Expr Purif 39:144-151 Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402-408 Lo CF, Leu JH, Ho CH, Chen CH, Peng SE, Chen YT, Chou CM, Yeh PY, Huang CJ, Chou HY, Wang CH, Kou GH (1996) Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Dis Aquat Org 25:133-141 Luft T, Pang KC, Thomas E, Hertzog P, Hart DN, Trapani J, Cebon J (1998) Type I IFNs enhance the terminal differentiation of dendritic cells. J Immunol 161:1947-1953 Luna C, Hoa NT, Zhang J, Kanzok SM, Brown SE, Imler JL, Knudson DL, Zheng L (2003) Characterization of three Toll-like genes from mosquito Aedes aegypti. Insect Mol Biol 12:67-74 Medzhitov R, Janeway CA, Jr. (1997) Innate immunity: the virtues of a nonclonal system of recognition. 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Curr Opin Genet Dev 1:470-477 Pasare C, Medzhitov R (2005) Toll-like receptors: linking innate and adaptive immunity. Adv Exp Med Biol 560:11-18 Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, Beutler B (1998) Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282:2085-2088 Relf JM, Chisholm JR, Kemp GD, Smith VJ (1999) Purification and characterization of a cysteine-rich 11.5-kDa antibacterial protein from the granular haemocytes of the shore crab, Carcinus maenas. Eur J Biochem 264:350-357 Rogers JH (1990) The role of introns in evolution. FEBS Lett 268:339-343 Rutschmann S, Kilinc A, Ferrandon D (2002) Cutting edge: the toll pathway is required for resistance to gram-positive bacterial infections in Drosophila. J Immunol 168:1542-1546 Senger K, Armstrong GW, Rowell WJ, Kwan JM, Markstein M, Levine M (2004) Immunity regulatory DNAs share common organizational features in Drosophila. Mol Cell 13:19-32 Shi Y, Evans JE, Rock KL (2003) Molecular identification of a danger signal that alerts the immune system to dying cells. Nature 425:516-521 Shimazu R, Akashi S, Ogata H, Nagai Y, Fukudome K, Miyake K, Kimoto M (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med 189:1777-1782 Slack JL, Schooley K, Bonnert TP, Mitcham JL, Qwarnstrom EE, Sims JE, Dower SK (2000) Identification of two major sites in the type I interleukin-1 receptor cytoplasmic region responsible for coupling to pro-inflammatory signaling pathways. J Biol Chem 275:4670-4678 Takeda K, Kaisho T, Akira S (2003) Toll-like receptors. Annu Rev Immunol 21:335-376 Tauszig S, Jouanguy E, Hoffmann JA, Imler JL (2000) Toll-related receptors and the control of antimicrobial peptide expression in Drosophila. Proc Natl Acad Sci U S A 97:10520-10525 Thisse C, Perrin-Schmitt F, Stoetzel C, Thisse B (1991) Sequence-specific transactivation of the Drosophila twist gene by the dorsal gene product. Cell 65:1191-1201 Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882 Toshchakov V, Jones BW, Perera PY, Thomas K, Cody MJ, Zhang S, Williams BR, Major J, Hamilton TA, Fenton MJ, Vogel SN (2002) TLR4, but not TLR2, mediates IFN-beta-induced STAT1alpha/beta-dependent gene expression in macrophages. Nat Immunol 3:392-398 Tzou P, Ohresser S, Ferrandon D, Capovilla M, Reichhart JM, Lemaitre B, Hoffmann JA, Imler JL (2000) Tissue-specific inducible expression of antimicrobial peptide genes in Drosophila surface epithelia. Immunity 13:737-748 Vargas-Albores F, Yepiz-Plascencia G, Jimenez-Vega F, Avila-Villa A (2004) Structural and functional differences of Litopenaeus vannamei crustins. Comp Biochem Physiol B Biochem Mol Biol 138:415-422 Winans KA, Hashimoto C (1995) Ventralization of the Drosophila embryo by deletion of extracellular leucine-rich repeats in the Toll protein. Mol Biol Cell 6:587-596 Yamagata M, Merlie JP, Sanes JR (1994) Interspecific comparisons reveal conserved features of the Drosophila Toll protein. Gene 139:223-228 Yang LS, Yin ZX, Liao JX, Huang XD, Guo CJ, Weng SP, Chan SM, Yu XQ, He JG (2006) A Toll receptor in shrimp. Mol Immunol Zambon RA, Nandakumar M, Vakharia VN, Wu LP (2005) The Toll pathway is important for an antiviral response in Drosophila. Proc Natl Acad Sci U S A 102:7257-7262 Zheng L, Zhang, L., Lin, H., McIntosh, M.T., Malacrida, A.R. (2005) Toll-like receptors in invertebrate innate immunity. ISJ 2:105-113 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26921 | - |
| dc.description.abstract | 對蝦類乃是重要的水產養殖物種，藉由瞭解其免疫系統有助於增進對疾病之控制。在先天性免疫系統中，Toll 途徑是用以感知病原體並激活免疫反應的主要方式之ㄧ。本研究中，從草蝦選殖出其 Toll 基因 (PmToll) 之 cDNA 全長並對其序列進行分析。PmToll 之編碼區 (coding region) 中含有 4 個內插子 (intron)，其聚胜肽鏈由 931 個胺基酸所組成，並包含 Toll 家族分子明確之特徵：富含白胺酸的重複序列 (leucine-rich repeat motifs, LRR motifs) 以及保守的 Toll/interleukin-1 receptor (TIR) domain。PmToll 之 mRNA 可在胃、鰓、血球、類淋巴器官、肝胰腺、腸、神經組織與表皮等處偵測到。利用定量即時 PCR 之方法可知，在受革蘭氏陽性菌與真菌之肽聚醣刺激後，南美白蝦 (Litopenaeus vannamei) Toll (LvToll) 之 mRNA 表現量上升，而 immune deficiency (Imd) 基因之 mRNA 表現量則無顯著改變。而在受真菌肽聚醣刺激之蝦體中，一個可能由 Toll 途徑所調控之抗微生物胜肽基因－penaeidin 4-1 (PEN4)，其 mRNA 表現量亦上升。此結果暗示著對蝦類 Toll 途徑可能藉由調控 PEN4 使其表現量上升以對抗真菌之入侵。但其中之分子機制仍需進一步的實驗證明之。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:32:28Z (GMT). No. of bitstreams: 1 ntu-97-R95b41002-1.pdf: 1571624 bytes, checksum: 32f05a9fe46ff24c275cace1bf602e0b (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 一、文獻探討．．．．．．．．．．．．．．．．．．．．．．．．．．．．１
1.1 Toll 與 Toll-like receptor 分子．．．．．．．．．．．．．．．．．．１ 1.2哺乳類 TLR 途徑之功能．．．．．．．．．．．．．．．．．．．．２ 1.3果蠅 Toll 途徑之功能．．．．．．．．．．．．．．．．．．．．．２ 1.4抗微生物胜肽．．．．．．．．．．．．．．．．．．．．．．．．．３ 1.5對蝦類中 Toll 分子之研究現況 ．．．．．．．．．．．．．．．．．５ 二、研究動機．．．．．．．．．．．．．．．．．．．．．．．．．．．．６ 三、材料與方法．．．．．．．．．．．．．．．．．．．．．．．．．．．７ 3.1草蝦 Toll（PmToll）基因之選殖．．．．．．．．．．．．．．．．．７ 3.1.1樣本之製備．．．．．．．．．．．．．．．．．．．．．．．．７ 3.1.2組織全 RNA 之萃取．．．．．．．．．．．．．．．．．．．．７ 3.1.3互補 DNA（complementary DNA, cDNA）之合成．．．．．．．７ 3.1.4 PmToll 部分序列之選殖．．．．．．．．．．．．．．．．．．８ 3.1.5 5’/3’ 快速增幅cDNA末端（rapid amplification of 5’/3’ cDNA ends, 5’/3’ RACE）．．．．．．．．．．．．．．．．．．．．．．．８ 3.1.6 PmToll cDNA 全長之增幅．．．．．．．．．．．．．．．．．９ 3.2 PmToll 基因體結構（genomic structure）之鑑定．．．．．．．．．．９ 3.3 PmToll 之序列分析．．．．．．．．．．．．．．．．．．．．．．９ 3.4 PmToll 轉錄本（transcript）組織分佈之分析．．．．．．．．．．．１０ 3.4.1樣本之製備．．．．．．．．．．．．．．．．．．．．．．１０ 3.4.2組織全 RNA 之萃取．．．．．．．．．．．．．．．．．．１０ 3.4.3反轉錄－聚合酶鏈鎖反應（reverse transcription- polymerase chain reaction, RT-PCR）．．．．．．．．．．．．．．．．．．．１０ 3.5 PmToll 之離體（in vitro）功能性分析．．．．．．．．．．．．．１１ 3.5.1質體之構築．．．．．．．．．．．．．．．．．．．．．．１１ 3.5.2轉染作用（transfection）．．．．．．．．．．．．．．．．．１２ 3.5.3冷光之測量與其數值之計算．．．．．．．．．．．．．．．１２ 3.5.4西方轉印法（Western blot assay）．．．．．．．．．．．．．１２ 3.6對蝦類 Toll 之活體（in vivo）功能性分析．．．．．．．．．．．１３ 3.6.1樣品之製備．．．．．．．．．．．．．．．．．．．．．．１３ 3.6.2組織全 RNA 之萃取．．．．．．．．．．．．．．．．．．１３ 3.6.3互補 DNA（complementary DNA, cDNA）之合成．．．．．．１３ 3.6.4定量即時PCR（Quantitative real-time PCR）．．．．．．．．１３ 3.6.5數據統計．．．．．．．．．．．．．．．．．．．．．．．１４ 四、結果．．．．．．．．．．．．．．．．．．．．．．．．．．．．１５ 4.1 PmToll 之選殖及序列分析．．．．．．．．．．．．．．．．．．１５ 4.2 PmToll 之基因體結構．．．．．．．．．．．．．．．．．．．．１６ 4.3 PmToll 轉錄本（transcript）之組織分佈．．．．．．．．．．．．１６ 4.4 PmToll 無啟動果蠅 AMP 基因啟動子之能力．．．．．．．．．．１６ 4.5 LvToll 之 mRNA 表現量在肽聚醣刺激後上升．．．．．．．．．１７ 4.6 PEN4 轉錄本在真菌之肽聚醣刺激後表現量上升．．．．．．．．１７ 五、討論．．．．．．．．．．．．．．．．．．．．．．．．．．．．１９ 六、結論．．．．．．．．．．．．．．．．．．．．．．．．．．．．２４ 七、參考文獻．．．．．．．．．．．．．．．．．．．．．．．．．．２５ 八、表與圖．．．．．．．．．．．．．．．．．．．．．．．．．．．３１ 九、附錄．．．．．．．．．．．．．．．．．．．．．．．．．．．．４１ | |
| dc.language.iso | zh-TW | |
| dc.subject | 抗微生物胜肽 | zh_TW |
| dc.subject | 對蝦 | zh_TW |
| dc.subject | 先天性免疫系統 | zh_TW |
| dc.subject | 脂多醣 | zh_TW |
| dc.subject | 聚醣 | zh_TW |
| dc.subject | peptidoglycan | en |
| dc.subject | antimicrobial peptide | en |
| dc.subject | shrimp | en |
| dc.subject | innate immune system | en |
| dc.subject | lipopolysaccharide | en |
| dc.title | 草蝦 Toll receptor 之鑑定與分析 | zh_TW |
| dc.title | Identification and analysis of Penaeus monodon
Toll receptor | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 郭光雄 | |
| dc.contributor.oralexamcommittee | 王重雄,周信佑 | |
| dc.subject.keyword | 對蝦,先天性免疫系統,脂多醣,肽,聚醣,抗微生物胜肽, | zh_TW |
| dc.subject.keyword | shrimp,innate immune system,lipopolysaccharide,peptidoglycan,antimicrobial peptide, | en |
| dc.relation.page | 42 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2008-06-23 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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