請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26822完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡懷楨(Huaiu-Jen Tsai) | |
| dc.contributor.author | Shih-Hung Chang | en |
| dc.contributor.author | 張世弘 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:27:23Z | - |
| dc.date.copyright | 2008-07-17 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-10 | |
| dc.identifier.citation | 陳紫媖, 蘇惠美. (2005). 水產種苗的生產. 科學發展385: 1-10.
陳彥達. (2006). 電穿孔法在豐年蝦之基因轉殖研究. 國立台灣大學漁業科學研究所碩士論文. 陳曜鴻, 李文志, 蔡懷楨. (1999). 九孔β-tubulin 基因的選殖及分子 結構分析. 生物技術在水產養殖上應用論文集 3: 59-66. 蔡奇立. (1993). Gene Transfer of Oyster (Crassostrea gigas Thunberg), hard clam and brine shrmp (Artemia salina Linnaeus) by electroporaton. 國立台灣大學漁業科學研究所養殖組碩士論文. Arenal A., Pimentel R., García C., Pimentel E., and Aleström P. (2004). The SV40 T antigen nuclear localization sequence enhances nuclear import of vector DNA in embryos of a crustacean (Litopenaeus schmitti). Gene 337: 71-77. Boshart M., Weber F., Jahn G., Dorsch-Häsler K., Fleckenstein B., and Schaffner W. (1985). A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41: 521-530. Browne R.A., and Sorgeloos P. (1991). Experimental biology of cyst diapause. In Artemia Biology, edited by Browne, R. A., Sorgeloos, P., and Trotman, C. N. CRC Press, Boca Raton, FL, pp 93-118. Chen S. L., Li S. S., H. R., and Tsai H. J. (2008). Conditional production of a funtional fish growth hormone in the transgenic line of Nannochloropsis Oculata (Eustigmatophyceae). Journal of Phycology 44: 768-776. Chourrout D., Guyomard R., and Houdebine L. M. (1986). High efficiency gene transfer in rainbow trout (Salmo gairdneri Rich) by microinjection into egg cytoplasm. Aquaculture 51: 143– 150. Clegg J. S. (2001). Cryptobiosis- a peculiar state of biological organization. Comparative Biochemistry and Physiology 128B: 613-624. Clegg J. S. and Conte F. P. (1980). A review of the cellular and developmental biology of Artemia. In The Brine Shrimp Artemia vol.2, edited by Persoone G.., Sorgeloos P., Roels O., and Jaspers E., Universa Press, Wetteren, Belgium, pp. 11-54. Clegg J. S., and Trotman C. N. A. (2002). Physiological and biochemical aspects of Artemia Ecology. In Artemia: Basic and Applied Biology, edited by Abatzopoulos T. J., Beardmore J. A., Clegg J. S., and Sorgeloos P. Kluwer Academic Publishers, Dordrecht, pp 129–170. Copf T., Schro¨ der R. and Averof M. (2004). Ancestral role of caudal genes in axis elongation and segmentation. Proceedings of National Academy Sciences U.S.A. 101: 17711-17715. Debuchy R., Purton S., and Rochaix J. D. (1989). The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. The EMBO Journal 8: 2803-2809. Devlin R. H., Yesaki T. Y., Biagi C. A., Donaldson E. M., and Swanson P. (1994). Extraordinary salmon growth. Nature 371: 209–210. Drewes C. (2005). Quantitative investigations of hatching in brine shrimp cysts In ABLE, edited by Drewes C. Iowa, pp 1-11. Evjemo J. O., Coutteau P., Olsen Y., and Sorgeloos P. (1997). The stability of docosahexaenoic acid in two Artemia species following enrichment and subsequent starvation. Aquaculture 155: 135-148. Fromm M. E., Taylor L. P., and Walbot V. (1986). Stable transformation of maize after gene transfer by electroporation. Nature 319: 791-793. Gendreau S., Lardans V., Cadoret J. P., and Mialhe E. (1995). Transient expression of a luciferase reporter gene after ballistic introduction into Artemia franciscana (Crustacea) embryos. Aquaculture 133: 199-205. Inoue K., Yamashit S., Hata J., Kabeno S., Asada S., Nagahisa E., and Fujita T. (1990). Electroporation as a new technique for producing transgenic fish. Cell Differentiation and Development 29: 123– 128. Keilin D. (1959). The problem of anabiosis or latent life: history and current concept. Proceeding Royal Society London 150B: 149-191. Kindle K. L., Schnell R. A., Fernandez E., and Lefebvre P. A. (1989). Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. Journal of Cell Biology 109: 2589-2601. Kolkovski, S. (2001). Digestive enzymes in fish larvae and juveniles—implications and applications to formulated diets. Aquaculture 200: 181– 202. Kolkovski, S., Arieli, A., and Tandler, A. (1997a). Visual and chemical cues stimulate microdiet ingestion in gilthead seabream, Sparus aurata, larvae. Aquaculture International 5: 527–536. Kolkovski, S., Koven, W.M., and Tandler, A., (1997b). The mode of action of Artemia in enhancing utilization of microdiet by gilthead seabream Sparus aurata larvae. Aquaculture 155: 193–205. Lavens, P., and Sorgeloos, P. (2000). The history, present status and prospects of the availability of Artemia cysts for aquaculture. Aquaculture 181: 397–403. Lavitrano M., Camaioni A., Fazio V. M., Dolci S., Farace M.G., and Spadafora C. (1989). Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell 57:717–723. Lewis L. D., DeCamillis A. M., Brunetti R. C., Halder G., Kassner A.V., Selegue E. J., Higgs S., and Carroll B. S. (1999). Ectopic gene expression and homeotic transformations in arthropods using recombinant Sindbis viruses. Current Biology 9: 1279-1287. Lin C. C., Lin J. H. Y., Chen M. S., and Yang H. L. (2007). An oral nervous necrosis virus vaccine that induces protective immunity in larvae of grouper (Epinephelus coioides). Aquaculture 268: 265–273 Lubon H., Paleyanda R. K., Velander W. H., and Drohan W. N. (1996). Blood proteins from transgenic animal bioreactors. Transfusion Medicine Reviews 10: 131–143. Martinez R., Estrada M. P., Berlanga J., Guillen I., and Hernandez O. (1996). Growth enhancement in transgenic tilapia by ectopic expression of tilapia growth hormone. Molecular Marine Biology Biotechnoloy 5: 62–70. Munilla-Moran R., Stark J. R., and Barbour A. (1990). The role of exogenous enzymes in digestion in cultured turbot larvae Scophthalmus maximus. Aquaculture 88: 337– 350. Mayfield S., Franklin S., and Lerner R. (2003) Expression and assembly of a fully a ctive antibody in algae. Proceedings of National Academy Sciences U.S.A. 100(2):438-42. Nam Y. K., Noh J. K., Cho Y. S., Cho H. J., and Cho K. N. (2001). Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis. Transgenic Research 10: 353–362. Nambu Z., Tanaka S., and Nambu F. (2004). Influence of photoperiod and temperature on reproductive mode in the brine shrimp, Artemia franciscana. Journal of Experimental Zoology Comparative Experimental Biology 301: 542-546. Nunes S. B., Carvalho D. F., Guilhermino M. L., and Stappen V. G. (2006). Use of the genus Artemia in ecotoxicity testing. Environmental Pollution 144: 453-462. Paleyanda R. K., Velander W. H., Lee T. K., Scandella D. H., and Gwazdauskas F.C. (1997). Transgenic pigs produce functional human factor VIII in milk. Nature Biotechnology 15: 971–975. Palmiter R. D., Brinster R. L., Hammer R. E., Trumbauer M. E., Rosenfeld M. G., Birnberg N. C., and Evans R. M. (1982). Dramatic growth of mice that develop from eggs microinjected with metallothionein−growth hormone fusion genes. Nature 294: 611-615. Pavlopoulos A., and Averof M. (2005). Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis. Proceedings of National Academy Sciences U.S.A. 102: 7888-7893. Papadopoulos A. I., Lazaridou A E., Mauridou A G., and Touraki M. (2004). Glutathione S-transferase in the branchiopod Artemia salina. Marine Biology 144: 295-301. Pitkanen T. I., Krasnov A., Teerijoki H., and Molsa H. (1999). Transfer of growth hormone (GH) transgenes into Arctic charr (Salvelinus alpinus L.) Growth response to various GH constructs. Genetic Analysis 15: 91–98. Rembold M., Lahiri K., Foulkes N. S., and Wittbrodt J. (2006). Transgenesis in fish : efficient selection of transgenic fish by co-injection with a fluorescent reporter construct. Nature Protocol 3: 1133-1139. Robinson C. D., Lourido S., Whlan S. P., Didycha J. L., Lych M., and Isern S. (2006). Viral Transgenesis of embryonic cell cultures from the freshwater microcrustacean Daphnia. Journal of Experimental Zoology 305A:62–67. Sargent J. (1992). New developments in the omega-3 story from man to fish and heart to brain. Aquaculture News, July:4-5. Sanford J. C. (1988). The biolistic process. Trends in Biotechnology 6: 299-302. Secchi C., and Borromeo V. (1997). Structure and function of bovine growth hormone bovine growth hormone as an experimental model for studies of protein-protein interactions. Journal of Chromatography 688B:161-177. Sorgeloos P., Bossuyt E., Laviña E., Baeza-Mesa M., and Persoone G. (1977). Decapsulation of Artemia cysts: A simple technique for the improvement of the use of brine shrimp in aquaculture. Aquaculture 12: 311-315. Sorgeloos, P., Dhert, P., and Candreva, P. (2001). Use of the brine shrimp, Artemia spp., in marine fish larviculture. Aquaculture 200: 147– 160. Takagi A., Sasado T., Tamiya G., Ozato K., Wakamatsu Y., Takeshita A., and Kimura M. (1994). An efficient expression vector for transgenic medaka construction. Molecular Marine Biology Biotechnology 3: 192–199. Tanguay J. A., Reyes R. C., and Clegg J. S. 2004. Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia. Journal of Bioscience 29: 489–501. Tate W. P., and Marshall C. J. (1991). Post-dormancy transcription and translation in the brine shrimp. In Artemia Biology, edited by Browne, R. A., Sorgeloos, P., and Trotman, C. N. CRC Press, Boca Raton, FL, pp 21-36. Tseng F. S., Tsai H. J., Liao I.C., and Song Y. L. (2000). Introducing foreign DNA into tiger shrimp (Penaeus monodon) by electroporation. Theriogenology 54: 1421– 1432. Tsai H.J. (2000). Electroporated sperm mediation of a gene transfer system for finfish and shellfish. Molecular Reproduction and Development 56: 281– 284. Tsai H. J., Lin K. L., Kuo J. C., and Chen S. W. (1995). Highly Efficient Expression of Fish Growth Hormone by Escherichia coli Cells. Applied and Environmental Microbiology 116: 4116-4119. van Berkel P. H., Welling M. M., Geerts M. M., van Veen H. A., and Ravensbergen B. (2002). Large scale production of recombinant human lactoferin in the milk of transgenic cow. Nature Biotechnology 20: 484–487. Walker R. L., Buret A. G., Jackson C. L., Scott K.G. E., Bajwa R., and Habibi H. R. (2004). Effects of growth hormone on leucine absorption,intestinal morphology, and ultrastructure of the goldish intestine. Canadian Journal of Physiology and Pharmacology 82: 951-959. Walker T. L., Purton S., Becker D. K., and Collet C. (2005). Microalgae as bioreactors. Plant Cell Reports 11: 629-641. Watanabe T., Kitajima C., and Fujta S. (1983a). Nutritional value of live food organisms used in Japan for mass culture of fish : A review. Aquaculture 34 : 115-143. Watanabe T., Oowa F., Kitajima C., and Fujta S. (1978). Nutritional quality of brine shrimp, Artemia salina, as a living feed from the viewpoint of essential fatty acid for fish. In Bulletin of the Japanese Society of Scientific Fisheries 44: 1115-1121. Wood D. C., Salsgiver W. J., Kasser T. R., Lange G. W., Rowold E., Violand B. N., Johnson A., Leimgrnber R. M., Parr G. R., Siegel N. R., Kimack N. M., Smith C. E., Zobel J. F., Ganguli S. M., Garbow J. R., Bild G., and Krivi G.G. (1989).Purification and characterization of pituitary bovine somatotropin. The Journal of Biological Chemisry 264: 14741-14747. Web site: USGS (2001). United States Geological Survey- Great Salt Lake: http://www.usgs.gov/ | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26822 | - |
| dc.description.abstract | 甲殼類豐年蝦的無節幼蟲，是最廣泛被利用於餵食養殖魚蝦貝類的活體餌料生物。我們利用電穿孔轉殖於豐年蝦休眠卵中。觀察孵化後七天具有綠螢光之F0豐年蝦個體，其綠螢光表現率為13.3% (3219/24054)，進而選取200隻有強綠螢光表現的豐年蝦個體，再與野生型 (wild-type)進行配對後得到F1，選取F1豐年蝦中有綠螢光表現，進行近親自交得到F2，選取F2豐年蝦中有綠螢光表現，進行近親自交得到F3，並針對每一子代 (G0~F3)染色體DNA進行萃取，利用PCR偵測是否含有698 bp綠螢光基因PCR放大的片段及564 bp黃鰭鯛生長激素 PCR放大的片段，最後成功篩選出2株遺傳穩定的轉殖品系A3及A8。經SDS-PAGE分析後，發現在相同位置與野生型比對有明顯多出一條band，將該band切割下來進行LC-MS蛋白質定序，經過序列比對後，證實為重組黃鰭鯛生長激素rGH。並利用西方轉漬法偵測重組生長激素能在25 kDa 被偵測到。經ELISA 測定50隻來自轉殖豐年蝦A3及A8 homozygotic品系的無節幼蟲，其所含重組黃鰭鯛生長激素分別為0.089μg 及0.032μg。進一步地，斑馬魚(Danio rerio)於受精後第25天(dpf)的幼魚，分別施予每日餵食10隻野生型或轉殖豐年蝦，連續餵食十天後，結果顯示餵食野生型的組平均淨增長率為34.74% ;而餵食轉殖組的豐年蝦的平均淨增長率為53.24%，餵食轉殖組其平均體長比野生型組高出15.5%，具有顯著差異(p<0.01)，因此，我們成功地建立一種可以穩定表達外來基因的轉殖豐年蝦品系，且能經口服的方式促進魚苗生長。 | zh_TW |
| dc.description.abstract | Brine shrimp nauplii (Artemia sp.) are mostly used as the livefeed for many cultured marine shellfish and finfish larvae, and it has also been used in the biological study as a model organism. Yet, the transgenesis of artemia has not well documented. Here, we applied electroporation on artemia cysts to generate the transgenic artemia. After the artemia cysts were decapsulated, the cysts were then electroporated with two plasmids, in which one contained GFP reporter gene and the other contained yellowfin porgy growth hormone (ypGH) cDNA. The transient GFP signal shown in the artemia larvae was observed at 7-d after hatching and the expression rate was 13.3% (3219 out of 24054 examined). We chose 200 G0 individuals harboring a high GFP fluorescence to mate with wild-type. Among F1 offsprings, we sampled high GFP- positive individuals to generate F2, and thereof F3 generations of Artemia. Genomic DNAs were extracted from the G0, F1, F2 and F3 and were checked with PCR detection. Two stable lines of transgenic Artemia were screened, named A3 and A8, because a 698-bp and a 564-bp PCR product were amplified, which were correspondent with PCR from GFP gene and ypGH cDNA, respectively. We found that one extra protein band with 25-kDa was shown in the transgenic Artemia, when it was compared with the total protein profile of wild-type. This extra band was cut out and digested in-gel for LC-MS peptide mapping, resulting that the amino acid sequence was confirmed as the recombinant ypGH. In addition, this 25-kDa protein was positive for antiserum against ypGH .The concentrations of ypGH produced by 50 homozygotic nauplii from two transgenic Artemia lines A3 and A8 were calculated as 0.089μg and 0.032μg by indirect ELISA. We respectively fed zebrafish (Danio rerio) on 10 wild-type or transgenic Artemia nauplii once a day from 25 dpf (days of postfertilization) to 35 dpf. The wild-type group average body length gain rate was 34.74%. The transgenic group average body length gain rate was 53.24%. Transgenic Artemia was statistically 15.5% larger than feeding wild-type at 35 dpf (p<0.01). Therefore, we conclude that we successfully establish two transgenic lines of Artemia expressing exogenous genes and enhance fish growth by oral administration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:27:23Z (GMT). No. of bitstreams: 1 ntu-97-R95b43029-1.pdf: 1403175 bytes, checksum: 6308f02fb56b7f383cc77ddc15069230 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 中文摘要 (Abstract in Chinese)…………...…..…...…...… 1
英文摘要 (Abstract in English)…………...…..…..………. 2 前言 (Introduction)………………………..………………. 4 材料與方法 (Materials and methods) …….……………… 10 結果 (Results)…………………………….………………..21 討論 (Discussion)……………………….………………... 26 參考資料 (References)……………………..…………….. 32 圖與表 (Figures and tables) …………................................ 39 附錄(Appendix)……………………………………………. 49 | |
| dc.language.iso | zh-TW | |
| dc.subject | 基因轉殖 | zh_TW |
| dc.subject | 魚類生長激素 | zh_TW |
| dc.subject | 豐年蝦 | zh_TW |
| dc.subject | Artemia | en |
| dc.subject | transgene | en |
| dc.subject | fish growth hormone | en |
| dc.title | 基因轉殖豐年蝦：穩定遺傳品系之建立與應用 | zh_TW |
| dc.title | Transgenic Artemia: establishment and application
of stable-transmitted lines | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭登貴,蕭世民,嚴宏洋 | |
| dc.subject.keyword | 豐年蝦,基因轉殖,魚類生長激素, | zh_TW |
| dc.subject.keyword | Artemia,transgene,fish growth hormone, | en |
| dc.relation.page | 52 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2008-07-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-97-1.pdf 未授權公開取用 | 1.37 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。