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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26714Full metadata record
| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 林中梧 | |
| dc.contributor.author | Mi-Hsin Huang | en |
| dc.contributor.author | 黃宓昕 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:22:09Z | - |
| dc.date.copyright | 2008-08-14 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-24 | |
| dc.identifier.citation | 1. M.Beverly, K.Harsough, L.Machemer, P.Pavo, and J.Lochridge. 2006. Liquid chromatography electrospray ionization mass spectrometry analysis of the ocular metabolites from a short interfering RNA duplex. J chromatography B, 835:62-70.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26714 | - |
| dc.description.abstract | MicroRNA (miRNA) 是一種有調節能力的小分子的RNA，作為基因轉錄後的調控者。由於在基因表現上扮演重要角色，研究基因體 (genome)中miRNA的表現，傳統方法常使用定量反轉錄聚合酶鏈式反應(qRT-PCR) 或微陣列 (array) 分析miRNA。在這篇論文中，我們利用一種新的技術來分析基因體中miRNA的表現。藉由高效液相色層分析法(HPLC)純化出不同細胞株之miRNA，分別定序每一個選殖株 (clone)，並藉由生物資訊的方法來比對。每一個資料庫中約30-40%的選殖株為已知可信的miRNA | zh_TW |
| dc.description.abstract | MicroRNAs (miRNAs) are a class of small regulatory RNAs, which act as post-transcriptional modulators of gene expression. Because of their critical roles in gene expression, genome-wide profiling of miRNAs, by qRT-PCR or array, has become an essential step in understanding cellular biology. In this thesis, we have developed a novel method for constructing miRNA libraries, as an initial step toward genome-wide miRNA characterization. We purified and cloned miRNAs by HPLC from several cell lines. Individual clones were sequenced, and identified by bioinformatics. It was estimated that about 30-40% of the clones from each library represented authentic miRNAs. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:22:09Z (GMT). No. of bitstreams: 1 ntu-97-R95444003-1.pdf: 452682 bytes, checksum: 1c5f01d8b60b3336ba026224dc747027 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | Content
Acknowledgement 02 Abstract 03 Chinese Abstract 04 Chapter 1: Introduction 05 Chapter 2: Materials and Methods 09 Chapter 3: Results and Discussion 26 Table1 33 Table2 42 Figure1 46 Figure2 48 Figure3 49 Reference 50 | |
| dc.language.iso | en | |
| dc.subject | 資料庫 | zh_TW |
| dc.subject | 淋巴癌 | zh_TW |
| dc.subject | 霍奇金病 | zh_TW |
| dc.subject | microRNA | en |
| dc.subject | library | en |
| dc.subject | lymphoma | en |
| dc.subject | Hodgkin lymphoma | en |
| dc.title | 建立自然殺手細胞淋巴癌及霍奇金病細胞株的microRNA資料庫 | zh_TW |
| dc.title | Construction of microRNA libraries in NK-cell and Hodgkin lymphoma cell lines | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林欽塘,周綠蘋,吳漢忠 | |
| dc.subject.keyword | 淋巴癌,霍奇金病,資料庫, | zh_TW |
| dc.subject.keyword | lymphoma,Hodgkin lymphoma,microRNA,library, | en |
| dc.relation.page | 52 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2008-07-24 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 病理學研究所 | zh_TW |
| Appears in Collections: | 病理學科所 | |
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| File | Size | Format | |
|---|---|---|---|
| ntu-97-1.pdf Restricted Access | 442.07 kB | Adobe PDF |
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