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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 蔡懷楨 | |
dc.contributor.author | Jeng-Ting Liu | en |
dc.contributor.author | 劉政廷 | zh_TW |
dc.date.accessioned | 2021-06-08T07:21:55Z | - |
dc.date.copyright | 2008-07-26 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-24 | |
dc.identifier.citation | Amato, M.A., Boy, S., and Perron, M. (2004). Hedgehog signaling in vertebrate eye development: a growing puzzle. Cell Mol Life Sci 61, 899-910.
Amirthalingam, K., Lorens, J.B., Saetre, B.O., Salaneck, E., and Fjose, A. (1995). Embryonic expression and DNA-binding properties of zebrafish pax-6. Biochem Biophys Res Commun 215, 122-128. Bailey, T.J., El-Hodiri, H., Zhang, L., Shah, R., Mathers, P.H., and Jamrich, M. (2004). Regulation of vertebrate eye development by Rx genes. Int J Dev Biol 48, 761-770. Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A. (2005). Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina. Dev Biol 278, 381-395. Blader, P., Fischer, N., Gradwohl, G., Guillemot, F., and Strahle, U. (1997). The activity of neurogenin1 is controlled by local cues in the zebrafish embryo. Development 124, 4557-4569. Bovolenta, P., Mallamaci, A., Puelles, L., and Boncinelli, E. (1998). Expression pattern of cSix3, a member of the Six/sine oculis family of transcription factors. Mech Dev 70, 201-203. Burd, C.G., Strochlic, T.I., and Gangi Setty, S.R. (2004). Arf-like GTPases: not so Arf-like after all. Trends Cell Biol 14, 687-694. Carl, M., Loosli, F., and Wittbrodt, J. (2002). Six3 inactivation reveals its essential role for the formation and patterning of the vertebrate eye. Development 129, 4057-4063. Chow, R.L. and Lang, R.A. (2001). Early eye development in vertebrates. Annu Rev Cell Dev Biol 17, 255-296. Christensen, S.T. and Ott, C.M. (2007). Cell signaling. A ciliary signaling switch. Science 317, 330-331. Chuang, J.C., Mathers, P.H., and Raymond, P.A. (1999). Expression of three Rx homeobox genes in embryonic and adult zebrafish. Mech Dev 84, 195-198. Chuang, J.C. and Raymond, P.A. (2001). Zebrafish genes rx1 and rx2 help define the region of forebrain that gives rise to retina. Dev Biol 231, 13-30. D'Souza-Schorey, C. and Chavrier, P. (2006). ARF proteins: roles in membrane traffic and beyond. Nat Rev Mol Cell Biol 7, 347-358. Dyer, M.A. and Cepko, C.L. (2001). p27Kip1 and p57Kip2 regulate proliferation in distinct retinal progenitor cell populations. J Neurosci 21, 4259-4271. Easter, S.S. and Nicola, G.N. (1996). The development of vision in the zebrafish (Danio rerio). Dev Biol 180, 646-663. Fadool, J.M. and Dowling, J.E. (2008). Zebrafish: a model system for the study of eye genetics. Prog Retin Eye Res 27, 89-110. Fan, Y., Esmail, M.A., Ansley, S.J., Blacque, O.E., Boroevich, K., Ross, A.J., Moore, S.J., Badano, J.L., May-Simera, H., Compton, D.S., et al. (2004). Mutations in a member of the Ras superfamily of small GTP-binding proteins causes Bardet-Biedl syndrome. Nat Genet 36, 989-993. Fashena, D. and Westerfield, M. (1999). Secondary motoneuron axons localize DM-GRASP on their fasciculated segments. J Comp Neurol 406, 415-424. Gehring, W.J. and Ikeo, K. (1999). Pax 6: mastering eye morphogenesis and eye evolution. Trends Genet 15, 371-377. Harris, W.A. and Perron, M. (1998). Molecular recapitulation: the growth of the vertebrate retina. Int J Dev Biol 42, 299-304. Hu, M. and Easter, S.S. (1999). Retinal neurogenesis: the formation of the initial central patch of postmitotic cells. Dev Biol 207, 309-321. Huang, H.Y., Dai, ES., Liu, J.T and Tsai, H.J. (2008). The embryonic expression pattern and the knowdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Dev Dyn (Revised). Ingley, E., Williams, J.H., Walker, C.E., Tsai, S., Colley, S., Sayer, M.S., Tilbrook, P.A., Sarna, M., Beaumont, J.G., and Klinken, S.P. (1999). A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit. FEBS Lett 459, 69-74. Kahn, R.A., Volpicelli-Daley, L., Bowzard, B., Shrivastava-Ranjan, P., Li, Y., Zhou, C., and Cunningham, L. (2005). Arf family GTPases: roles in membrane traffic and microtubule dynamics. Biochem Soc Trans 33, 1269-1272. Kalies, K.U., Rapoport, T.A., and Hartmann, E. (1998). The beta subunit of the Sec61 complex facilitates cotranslational protein transport and interacts with the signal peptidase during translocation. J Cell Biol 141, 887-894. Kikuchi, Y., Segawa, H., Tokumoto, M., Tsubokawa, T., Hotta, Y., Uyemura, K., and Okamoto, H. (1997). Ocular and cerebellar defects in zebrafish induced by overexpression of the LIM domains of the islet-3 LIM/homeodomain protein. Neuron 18, 369-382. Kim, C.H., Ueshima, E., Muraoka, O., Tanaka, H., Yeo, S.Y., Huh, T.L., and Miki, N. (1996). Zebrafish elav/HuC homologue as a very early neuronal marker. Neurosci Lett 216, 109-112. Kimmel, C.B., Ballard, W.W., Kimmel, S.R., Ullmann, B., and Schilling, T.F. (1995). Stages of embryonic development of the zebrafish. Dev Dyn 203, 253-310. Kinch, L.N., Saier, M.H., and Grishin, N.V. (2002). Sec61beta--a component of the archaeal protein secretory system. Trends Biochem Sci 27, 170-171. Krauss, S., Johansen, T., Korzh, V., and Fjose, A. (1991). Expression of the zebrafish paired box gene pax[zf-b] during early neurogenesis. Development 113, 1193-1206. Li, H., Tierney, C., Wen, L., Wu, J.Y., and Rao, Y. (1997). A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate. Development 124, 603-615. Liu, Y., Shen, Y., Rest, J.S., Raymond, P.A., and Zack, D.J. (2001). Isolation and characterization of a zebrafish homologue of the cone rod homeobox gene. Invest Ophthalmol Vis Sci 42, 481-487. Loosli, F., Koster, R.W., Carl, M., Krone, A., and Wittbrodt, J. (1998). Six3, a medaka homologue of the Drosophila homeobox gene sine oculis is expressed in the anterior embryonic shield and the developing eye. Mech Dev 74, 159-164. Lopez-Rios, J., Tessmar, K., Loosli, F., Wittbrodt, J., and Bovolenta, P. (2003). Six3 and Six6 activity is modulated by members of the groucho family. Development 130, 185-195. Lui, H.M., Chen, J., Wang, L., and Naumovski, L. (2003). ARMER, apoptotic regulator in the membrane of the endoplasmic reticulum, a novel inhibitor of apoptosis. Mol Cancer Res 1, 508-518. Macdonald, R., Barth, K.A., Xu, Q., Holder, N., Mikkola, I., and Wilson, S.W. (1995). Midline signalling is required for Pax gene regulation and patterning of the eyes. Development 121, 3267-3278. Macdonald, R., Scholes, J., Strahle, U., Brennan, C., Holder, N., Brand, M., and Wilson, S.W. (1997). The Pax protein Noi is required for commissural axon pathway formation in the rostral forebrain. Development 124, 2397-2408. Malicki, J., Neuhauss, S.C., Schier, A.F., Solnica-Krezel, L., Stemple, D.L., Stainier, D.Y., Abdelilah, S., Zwartkruis, F., Rangini, Z., and Driever, W. (1996). Mutations affecting development of the zebrafish retina. Development 123, 263-273. Marquardt, T. and Gruss, P. (2002). Generating neuronal diversity in the retina: one for nearly all. Trends Neurosci 25, 32-38. Mathers, P.H., Grinberg, A., Mahon, K.A., and Jamrich, M. (1997). The Rx homeobox gene is essential for vertebrate eye development. Nature 387, 603-607. Mathers, P.H., Miller, A., Doniach, T., Dirksen, M.L., and Jamrich, M. (1995). Initiation of anterior head-specific gene expression in uncommitted ectoderm of Xenopus laevis by ammonium chloride. Dev Biol 171, 641-654. Nornes, S., Clarkson, M., Mikkola, I., Pedersen, M., Bardsley, A., Martinez, J.P., Krauss, S., and Johansen, T. (1998). Zebrafish contains two pax6 genes involved in eye development. Mech Dev 77, 185-196. Oliver, G., Loosli, F., Koster, R., Wittbrodt, J., and Gruss, P. (1996). Ectopic lens induction in fish in response to the murine homeobox gene Six3. Mech Dev 60, 233-239. Park, H.C., Hong, S.K., Kim, H.S., Kim, S.H., Yoon, E.J., Kim, C.H., Miki, N., and Huh, T.L. (2000). Structural comparison of zebrafish Elav/Hu and their differential expressions during neurogenesis. Neurosci Lett 279, 81-84. Pasqualato, S., Renault, L., and Cherfils, J. (2002). Arf, Arl, Arp and Sar proteins: a family of GTP-binding proteins with a structural device for 'front-back' communication. EMBO Rep 3, 1035-1041. Passini, M.A., Levine, E.M., Canger, A.K., Raymond, P.A., and Schechter, N. (1997). Vsx-1 and Vsx-2: differential expression of two paired-like homeobox genes during zebrafish and goldfish retinogenesis. J Comp Neurol 388, 495-505. Peterson, R.E., Fadool, J.M., McClintock, J., and Linser, P.J. (2001). Muller cell differentiation in the zebrafish neural retina: evidence of distinct early and late stages in cell maturation. J Comp Neurol 429, 530-540. Pettersson, M., Bessonova, M., Gu, H.F., Groop, L.C., and Jonsson, J.I. (2000). Characterization, chromosomal localization, and expression during hematopoietic differentiation of the gene encoding Arl6ip, ADP-ribosylation-like factor-6 interacting protein (ARL6). Genomics 68, 351-354. Puschel, A.W., Gruss, P., and Westerfield, M. (1992). Sequence and expression pattern of pax-6 are highly conserved between zebrafish and mice. Development 114, 643-651. Raymond, P.A., Barthel, L.K., Bernardos, R.L., and Perkowski, J.J. (2006). Molecular characterization of retinal stem cells and their niches in adult zebrafish. BMC Dev Biol 6, 36. Ross, A.J., May-Simera, H., Eichers, E.R., Kai, M., Hill, J., Jagger, D.J., Leitch, C.C., Chapple, J.P., Munro, P.M., Fisher, S., et al. (2005). Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates. Nat Genet 37, 1135-1140. Sampath, K., Rubinstein, A.L., Cheng, A.M., Liang, J.O., Fekany, K., Solnica-Krezel, L., Korzh, V., Halpern, M.E., and Wright, C.V. (1998). Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling. Nature 395, 185-189. Sato, T., Takahoko, M., and Okamoto, H. (2006). HuC:Kaede, a useful tool to label neural morphologies in networks in vivo. Genesis 44, 136-142. Schmitt, E.A. and Dowling, J.E. (1994). Early eye morphogenesis in the zebrafish, Brachydanio rerio. J Comp Neurol 344, 532-542. Schmitt, E.A. and Dowling, J.E. (1999). Early retinal development in the zebrafish, Danio rerio: light and electron microscopic analyses. J Comp Neurol 404, 515-536. Schwarz, M., Cecconi, F., Bernier, G., Andrejewski, N., Kammandel, B., Wagner, M., and Gruss, P. (2000). Spatial specification of mammalian eye territories by reciprocal transcriptional repression of Pax2 and Pax6. Development 127, 4325-4334. Seo, H.C., Curtiss, J., Mlodzik, M., and Fjose, A. (1999). Six class homeobox genes in drosophila belong to three distinct families and are involved in head development. Mech Dev 83, 127-139. Seo, H.C., Drivenes, Ellingsen, S., and Fjose, A. (1998). Expression of two zebrafish homologues of the murine Six3 gene demarcates the initial eye primordia. Mech Dev 73, 45-57. Seo, H.C., Drivenes, O., Ellingsen, S., and Fjose, A. (1998). Transient expression of a novel Six3-related zebrafish gene during gastrulation and eye formation. Gene 216, 39-46. Shen, Y.C. and Raymond, P.A. (2004). Zebrafish cone-rod (crx) homeobox gene promotes retinogenesis. Dev Biol 269, 237-251. Shkumatava, A. and Neumann, C.J. (2005). Shh directs cell-cycle exit by activating p57Kip2 in the zebrafish retina. EMBO Rep 6, 563-569. Takada, T., Iida, K., Sasaki, H., Taira, M., and Kimura, H. (2005). Expression of ADP-ribosylation factor (ARF)-like protein 6 during mouse embryonic development. Int J Dev Biol 49, 891-894. Tobin, J.L. and Beales, P.L. (2007). Bardet-Biedl syndrome: beyond the cilium. Pediatr Nephrol 22, 926-936. Valcarcel, R., Weber, U., Jackson, D.B., Benes, V., Ansorge, W., Bohmann, D., and Mlodzik, M. (1999). Sec61beta, a subunit of the protein translocation channel, is required during Drosophila development. J Cell Sci 112 ( Pt 23), 4389-4396. Yamamoto, Y., Stock, D.W., and Jeffery, W.R. (2004). Hedgehog signalling controls eye degeneration in blind cavefish. Nature 431, 844-847. Zaghloul, N.A. and Moody, S.A. (2007). Alterations of rx1 and pax6 expression levels at neural plate stages differentially affect the production of retinal cell types and maintenance of retinal stem cell qualities. Dev Biol 306, 222-240. Zuber, M.E., Perron, M., Philpott, A., Bang, A., and Harris, W.A. (1999). Giant eyes in Xenopus laevis by overexpression of XOptx2. Cell 98, 341-352 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26709 | - |
dc.description.abstract | ADP-ribosylation factor-like 6 interacting protein (Arl6ip) 是母體效應基因,在原腸胚期前的斑馬魚胚胎中有全身性表現,發育到咽期後則會局部表現在特定組織;在Arl6ip缺失的斑馬魚中可以觀察到斑馬魚腦部、眼睛、心臟、肌肉有明顯的發育不良,特別是眼睛,出現小眼睛及視網膜細胞分化不良的情形。過去的研究認為Arl6ip與細胞的分化有密切關係,但它在發育上的功能卻完全未知;於是我們利用斑馬魚在發育生物學上的優勢來觀察眼部發育相關基因的表現情形,進而探討Arl6ip在眼睛發育時期調控的分子機制。當Arl6ip缺失時,早期決定眼部區域的基因表現受到影響,包括shh在頭部過量表現,以及rx1與pax6表現量降低,並且pax2高量表現於視柄,這都導致眼部區域的縮小。而在視網膜形成時期,觀察與視網膜發育相關的基因之表現情形,發現Arl6ip缺失後,斑馬魚胚胎視網膜細胞不能正常表現shh、six3a及six6等基因,代表視網膜細胞分化的過程受到影響;而HuC、neurolin及HNK-1等蛋白在視網膜的表現也都受到抑制,另外感光受體專一表現的基因crx也出現缺失,僅有少量訊號出現在視網膜腹側,顯示視網膜結構的確沒有出現;再者,利用deltaC標定視網膜前驅細胞,也可發現Arl6ip缺失後,眼部區域的細胞的確大量處於命運未被決定的形態。最後觀察眼部區域細胞增生的情形,我們也發現在Arl6ip缺失的斑馬魚胚胎中,眼部區域有大量BrdU的訊號,並且細胞大量表現cyclin D1,而不表現p57kip2,說明這些細胞無法離開細胞週期且無法進行分化,而處於早期形態。總結,當Arl6ip缺失時,斑馬魚視網膜的前驅細胞的分化會受到影響,使眼睛的發育停留在早期,並且這群眼睛的前驅細胞又因為細胞週期無法被停止而持續進行分裂;因此,我們認為Arl6ip影響視網膜前驅細胞離開細胞週期,並且擔任了眼部區域及視網膜細胞分化與成熟過程的重要功能。 | zh_TW |
dc.description.abstract | ADP-ribosylation factor-like 6 interacting protein (Arl6ip) was a maternally inherited gene in zebrafish, and it was expressed throughout the whole embryo before the gastrula period and restrictedly expressed in certain tissues after the pharyngula period. As losing Arl6ip, many phenotypic defects occurred in many tissues of the zebrafish embryo, such as brain, eyes, heart, and trunk. In particular, the occurrence of microphthalmos of eye and incomplete differentiation of retina were found. According to previous researches, Arl6ip was thought closely related to cell differentiation, but its biological function in development was unknow. Here we confer the molecular mechanism that Arl6ip involved in by observing the expression of related genes releated to zeberfish eyes development. First, we found that the expressions of early patterning genes were affected while losing Arl6ip, including overexpression of shh mRNA in the head, down-regulated of rx1 and pax6 mRNA in the eye field, and elevated expression of pax2 mRNA in optic stalk. These change in gene expression all result in the reduction of eye field. Then, we focued on the expressions of specific genes as retinal development. As losing Arl6ip, zebrafish embryos could not express shh, six3a, and six6 normally; that affected the differentiating processes of retina progenitor. Therefore, we could observe the specific proteins such as HuC, Neurolin, and HNK-1 were repressed in retina, and the photoreceptor specific gene crx was crowded at the ventral site of eyes. Furthermore, we labeled the cells in the eye field with deltaC and confirmed the cell fate of retinal progenitor kept indetermination while losing Arl6ip. Finally, we examined the level of cell proliferation in the eye field. While losing Arl6ip, we could observe the BrdU signals were higher in the eye, and the retinal progenitor kept expressing cyclin D1 but not p57kip2, suggesting the eye progenitor cell stayed as an early progenitor and could not exit cell cycle to process differentiation. Thus, inhibition of Arl6ip leads to arrest eye development at the early stage. These mal-functional eye progenitors keep proliferating and continually express early marker gene. Taken together, we conclude that arl6ip not only affects the signals controlling eye development, but also plays an important role in cell differentiation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:21:55Z (GMT). No. of bitstreams: 1 ntu-97-R95b43016-1.pdf: 7998896 bytes, checksum: 9a3ace14dfe0070556ba00f503361744 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 中文摘要………………………………………………………1
英文摘要………………………………………………………2 文獻回顧………………………………………………………3 前 言………………………………………………………14 實驗材料與方法………………………………………………17 實驗結果………………………………………………………23 討 論………………………………………………………32 結 論………………………………………………………41 參考文獻………………………………………………………42 圖 表………………………………………………………51 附 錄………………………………………………………63 | |
dc.language.iso | zh-TW | |
dc.title | ADP-Ribosylation Factor-like 6 Interacting Protein在斑馬魚眼睛發育功能的新發現 | zh_TW |
dc.title | Novel Function of ADP-Ribosylation Factor-like 6 Interacting Protein in the Ocular Development of Zebrafish Embryos | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 嚴宏洋,陳曜鴻,黃怡萱,焦傳金 | |
dc.subject.keyword | 斑馬魚,眼睛,發育, | zh_TW |
dc.subject.keyword | zebrafish,eye,development, | en |
dc.relation.page | 67 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2008-07-24 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
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