請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26704完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭石通(Shih-Tong Jeng) | |
| dc.contributor.author | Shih-Chieh Liang | en |
| dc.contributor.author | 梁詩婕 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:21:40Z | - |
| dc.date.copyright | 2008-08-11 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-22 | |
| dc.identifier.citation | 金禹析 (2006). 文心蘭老化相關基因OSAG243的釣取與分析。國立台灣大學植物學研究所碩士論文。
張萌惠 (2001). 百日咳抗原於轉殖菸草之表現與老鼠口服免疫反應。國立台灣大學植物學研究所碩士論文。 Alatzas, A., and Foundouli, A. (2006). Distribution of ubiquitinated histone H2A during plant cell differentiation in maize root and dedifferentiation in callus Culture. Plant Science 171, 481-487. Anne-Valerie Gendrel, Z.L., Rob Martinenssen and Vincent Colot. (2005). Profiling histone modification pattern in plants using genomic tiling microarray. Nature Method 2, 213-218. Ausin, I., Alonso-Blanco, C., Jarillo, J.A., Ruiz-Garcia, L., and Martinez-Zapater, J.M. (2004). Regulation of flowering time by FVE, a retinoblastoma-associated protein. Nature Genetics 36, 162-166. Bäurle, I., and Dean, C. (2006). The timing of developmental transitions in plants. Cell 125, 655-664. Bartova, E., Krejci, J., Harnicarova, A., Galiova, G., and Kozubek, S. (2008). Histone modifications and nuclear architecture: a review. journal of histochemistry and cytochemistry 20, 3-34. Dennis, E.S., and Peacock, W.J. (2007). Epigenetic regulation of flowering. Current Opinion in Plant Biology 10, 520-527. Fleury, D., Himanen, K., Cnops, G., Nelissen, H., Boccardi, T.M., Maere, S., Beemster, G.T.S., Neyt, P., Anami, S., Robles, P., Micol, J.L., Inze´, D., and Lijsebettensa, M.V. (2007). The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. The Plant Cell 19, 417–432. Fukuda, H., Sano, N., Muto, S., and Horikoshi, M. (2006). Simple histone acetylation plays a complex role in the regulation of gene expression. Brief Funct Genomic Proteomic 5, 190-208. He, Y., and Amasino, R.M. (2005). Role of Chromatin Modification in Flowering-time Control. Trends in Plant Science 10, 30-35. He, Y., Michaels, S.D., and Amasino, R.M. (2003). Regulation of Flowering Time by Histone Acetylation in Arabidopsis. Science 302, 1751-1754. He, Y., Doyle, M.R., and Amasino, R.M. (2004). PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin is required for the vernalization-responsive, winter-annual habit in Arabidopsis. Genes and Development 18, 2772-2784. Joazeiro, C.A.P., and Weissman, A.M. (2000). RING Finger Proteins: Mediators of Ubiquitin Ligase Activity. Cell 102, 549-552. Lazaro, A., Gomez-Zambrano, A., Lopez-Gonzalez, L., Pineiro, M., and Jarillo, J.A. (2008). Mutations in the Arabidopsis SWC6 Gene, Encoding a Component of the SWR1 Chromatin Remodelling Complex, Accelerate Fowering Time and Alter Leaf and Flower Development. Journal of Experimental Botany 59, 653-666 Liu, Y., Koornneef, M., and Soppea, W.J.J. (2007). The Absence of Histone H2B Monoubiquitination in the Arabidopsis hub1 (rdo4) Mutant Reveals a Role for Chromatin Remodeling in Seed Dormancy. The Plant Cell 19, 433–444. Michaels, S.D., and Amasino, R.M. (1999). FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering. The Plant Cell 11, 949-956. Michaels, S.D., and Amasino, R.M. (2000). Memories of Winter: Vernalization and the Competence to Flower. Plant, Cell and Environment 23, 1145-1153. Nelissen, H., Boccardi, T.M., Himanen, K., and Van Lijsebettens, M. (2007). Impact of Core Histone Modifications on Transcriptional Regulation and Plant Growth. Critical Reviews in Plant Sciences 26, 243-263. Platta, H.W., Magraoui, F.E., Schlee, D., Grunau, S., Girzalsky, W., and Erdmann, R. (2007). Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J. Cell Biol. 177, 197-204. Quesada, V., Macknight, R., Dean, C., and Simpson, G.G. (2003). Autoregulation of FCA pre-mRNA Processing Controls Arabidopsis Flowering Time. The EMBO Journal 22, 3142-3152. Ruth Bastow, J.S.M., Clare Lister, Zachary Lippman, Robert A. Martienssen & Caroline Dean. (2004). Vernalization Requires Epigenetic Silencing of FLC by Histone Methylation. Nature 427, 164-167. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press). Santos-Rosa, H., Schneider, R., Bannister, A.J., Sherriff, J., Bernstein, B.E., Emre, N.C.T., Schreiber, S.L., Mellor, J., and Kouzarides, T. (2002). Active genes are tri-methylated at K4 of histone H3. Nature 419, 407-411. Searle, I., He, Y., Turck, F., Vincent, C., Fornara, F., Krober, S., Amasino, R.A., and Coupland, G. (2006). The Transcription factor FLC Confers aFlowering Response to Vernalization by Repressing Meristem Competence and Systemic Signaling in Arabidopsis. Genes and Development 20, 898-912. Sheldon, C.C., Rouse, D.T., Finnegan, E.J., Peacock, W.J., and Dennis, E.S. (2000). The Molecular Basis of Vernalization: the Central Role of FLOWERING LOCUS C (FLC). Proceedings of the National Academy of Sciences 97, 3753-3758. Shilatifard, A. (2006). Chromatin Modifications by Methylation and Ubiquitination: Implications in the Regulation of Gene Expression. Annual Review of Biochemistry 75, 243-269. Shukla, A., and Bhaumik, S.R. (2007). H2B-K123 Ubiquitination Stimulates RNAPII Elongation Independent of H3-K4 Methylation. Biochemical and Biophysical Research Communications 39, 214-220. Simpson, G.G. (2004). The Autonomous Pathway: Epigenetic and Post-transcriptional Gene Regulation in the Control of Arabidopsis Flowering Time. Current Opinion in Plant Biology 7, 570-574. Sung, S., and Amasino, R.M. (2004). Vernalization in Arabidopsis thaliana is Mediated by the PHD Finger Protein VIN3. Nature 427, 159-164. Wang, J., Tian, L., Lee, H.-S., and Chen, Z.J. (2006). Nonadditive Regulation of FRI and FLC Loci Mediates Flowering-Time Variation in Arabidopsis Allopolyploids. Genetics 173, 965-974. Weake, V.M., and Workman, J.L. (2008). Histone Ubiquitination: Triggering Gene Activity. Molecular Cell 29, 653-663. Wood, A., Schneider, J., Dover, J., Johnston, M., and Shilatifard, A. (2003a). The Paf1 Complex Is Essential for Histone Monoubiquitination by the Rad6-Bre1 Complex, Which Signals for Histone Methylation by COMPASS and Dot1p. Journal of Biological Chemistry 278, 34739-34742. Wood, A., Krogan, N.J., Dover, J., Schneider, J., Heidt, J., Boateng, M.A., Dean, K., Golshani, A., Zhang, Y., Greenblatt, J.F., Johnston, M., and Shilatifard, A. (2003b). Bre1, an E3 Ubiquitin Ligase Required for Recruitment and Substrate Selection of Rad6 at a Promoter. Molecular Cell 11, 267-274. Xiao, T., Kao, C.-F., Krogan, N.J., Sun, Z.-W., Greenblatt, J.F., Osley, M.A., and Strahl, B.D. (2005). Histone H2B Ubiquitylation Is Associated with Elongating RNA Polymerase II. Molecular and Cellular Biology, 637–651. Yanovsky, M.J., and Kay, S.A. (2002). Molecular Basis of Seasonal Time Measurement in Arabidopsis. Nature 419, 308-312. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26704 | - |
| dc.description.abstract | 植物配合時令，在適當的時機開花結果是十分重要的。阿拉伯芥在接收各種環境因子之後，再藉由訊息傳導調控FLOWERING LOCUS C (FLC)表現來調控花期。在阿拉伯芥開花訊息傳導的路徑中，FLC扮演著主要的負調控者角色，而它的表現量和FLC啟動子區域的組蛋白修飾有關。本實驗室在阿拉伯芥中分離了一個和酵母菌BER1同源的基因HUB1，它與酵母菌BRE1同樣，具有E3泛素連結酶的功能，且它們的目標都是組蛋白H2B，在酵母菌的研究中發現當H2B接上泛素之後，會招來COMPASS(complex of proteins associated with Set1)複合體，並對附近的另一個組蛋白H3的第四個賴胺酸作三甲基化的修飾(H3K4 tri-methylation)，這樣的組蛋白後修飾組合會促進下游基因的表現。本實驗室發現T-DNA插入突變株hub1具有提早開花的性狀，而控制開花的主要調控者FLOWERING LOCUS C(FLC)的表現量較野生型阿拉伯芥為少。利用染色質免疫沉澱法觀察FLC啟動子區域組蛋白H3K4發生三甲基化之程度，發現若HUB1失去功能，三甲基化程度減少，同時泛素化的程度也降低，我們推測HUB1在阿拉伯芥開花生理調控的角色和酵母菌的BRE1調控基因表現的模式相同，它將單一泛素連結在FLC啟動子區域組蛋白H2B上之後，會促進H3K4發生三甲基化，活化FLC的表現，抑制植物開花，而在hub1植株中，FLC啟動子區域組蛋白活化性修飾減少，FLC表現因此下降，植物失去FLC的抑制，開花即提早發生。 | zh_TW |
| dc.description.abstract | FLOWERING LOCUS C (FLC), a central floral repressor in Arabidopsis thaliana, inhibits the expression of flowering genens, such as FLOWERING LOCUS T (FT) and SUPPRESSOR OF CONSTANT1 (SOC1). One of the mechanisms regulating FLC expression is epigenetic control, which includes histone modifications in gene’s promoter region. We isolated HISTONE MONO-UBIQUITINATION(HUB1) gene from Arabidopsis thaliana, which is a homolog of BRE1 in Saccharomyces cerevisiae. HUB1, with a RING-finger domain in its C-terminus, has E3 ligase activity, while its target, as that of BRE1 in yeast, is histone H2B. In yeast model, ubiquitination on H2B might induce H3K4 tri-methylation via COMPASS complex, and activate gene expression. T-DNA insertion plant with hub1 mutant shows early flowering. Besides, FLC expression level decreases, and its downstream flowering genes, FT and SOC1 are activated in hub1. Furthermore, Chromatin immunoprecipitation (ChIP) was performed, and a dramatic decrease in H3K4 tri-methylation and ubiquitylation was observed in FLC promoter. We propose that HUB1-mediated H2B ubiquitination might induce tri-methylation on H3K4, and consequently activate FLC expression | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:21:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2008 | en |
| dc.description.tableofcontents | 誌謝……………………………………………………………………………4
縮寫表…………………………………………………………………………5 中文摘要………………………………………………………………………6 英文摘要………………………………………………………………………7 第一章 前言……………………………………………………………………8 第二章 材料與方法…………………………………………………………14 一、材料………………………………………………………………………14 二、常用實驗方法……………………………………………………………14 三、質體構築與挑選…………………………………………………………17 四、掃描式電子顯微鏡觀察……………………………………………………18 五、植物基因組DNA抽取……………………………………………………18 六、RNA萃取…………………………………………………………………19 七、RT-PCR和real-timePCR………………………………………………20 八、阿拉伯芥組蛋白抽取……………………………………………………21 九、蛋白質分析………………………………………………………………22 十、染色質免疫沉澱…………………………………………………………25 第三章 結果……………………………………………………………………28 hub1有提早開花的現象………………………………………………………28 hub1開花相關基因表現情形………………………………………………28 hub1的組蛋白活化性修飾H3K4三甲基化有明顯下降情形…………………29 hub1的FLC啟動子區域的組蛋白修飾情形…………………………………29 第四章 討論………………………………………………………………………31 hub1有提早開花的性狀…………………………………………………………31 FLC的表現量與啟動子的表觀遺傳調控……………………………………31 HUB1如何調控特定基因………………………………………………………32 沉默的HUB1大量表現轉殖株OE1-1…………………………………………33 泛素化還是甲基化？…………………………………………………………33 結論………………………………………………………………………………34 參考文獻…………………………………………………………………………35 圖表………………………………………………………………………………42 | |
| dc.language.iso | zh-TW | |
| dc.title | 酵母菌BRE1同源基因HUB1於阿拉伯芥開花生理調控路徑之功能研究 | zh_TW |
| dc.title | HUB1, a homolog of BRE1 in Saccharomyces cerevisiae, plays a role in flowering time controlling in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 邱子珍(Tzyy-Jen Chiou),鄭秋萍(Chiu-Ping Cheng),吳克強(Keqiang Wu) | |
| dc.subject.keyword | 組蛋白修飾,開花調控, | zh_TW |
| dc.subject.keyword | epigenetic regulation,FLC,histone modification, | en |
| dc.relation.page | 34 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2008-07-24 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。