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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26681Full metadata record
| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 林中梧(Chung-Wu Lin) | |
| dc.contributor.author | Fai-Yun Lo | en |
| dc.contributor.author | 羅斐云 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:20:37Z | - |
| dc.date.copyright | 2008-08-08 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-24 | |
| dc.identifier.citation | 1. Trinchieri, G. Biology of natural killer cells. Adv. Immunol. 47, 187–376 (1989).
2. Kuby Immunology, 6th edition, by Thomas J. Kindt, Richard A. Goldsby,and Barbara A.Kuby W.H. Freeman and Company,New York 3. Cooper MA, Fehniger TA, Caligiuri MA. The biology of human natural killer-cell subsets. Trends in Immunology ) 22:633–40 ( 2001). 4. Ferlazzo G, Thomas D, Lin SL, et al. The abundant NK cells in human secondary lymphoid tissues require activation to express killer cell Ig-like receptors and become cytolytic. J Immunol 172:1455–62 (2004). 5. Robertson, M.J. et al. Human naturalkiller-cell adhesion molecules. Differential expression after activation and participation in cytolysis. J. Immunol. 145, 3194–3201 (1990). 6. Loza MJ, Perussia B. The IL-12 signature: NK cell terminal CD56+high stage and effector functions. J Immunol 172:88–96 ( 2004). 7. Fehniger, T.A. and Caligiuri, M.A. Interleukin-15: biology and relevance to human disease. Blood 97, 14–32 (2001). 8. Farag SS, Caligiuri MA. Human natural killer cell development and biology. Blood Reviews 20, 123–137 (2006). 9. Cooper MA, Fehniger TA, Turner SC, et al. Human natural killer cells: a unique innate immunoregulatory role for the CD56 (bright) subset. Blood 97:3146–51 (2001). 10. Perussia B, Chen Y, Loza MJ. Peripheral NK cell phenotypes: multiple changing of faces of an adapting, developing cell. Mol Immunol 42:385–95 (2005). 11. Nagler A, Lanier LL, Phillips JH. Constitutive expression of high affinity interleukin 2 receptors on human CD16- natural killer cells in vivo. J Exp Med 171:1527–33 (1990). 12. Robertson MJ, Soiffer RJ, Wolf SF, et al. Response of human natural killer (NK) cells to NK cell stimulatory factor (NKSF): cytolytic activity and proliferation of NK cells are differentially regulated by NKSF. J Exp Med 175: 779–88 (1992). 13. Caligiuri MA, Murray C, Robertson MJ, et al. Selective modulation of human natural killer cells in vivo after prolonged infusion of low dose recombinant interleukin 2. J Clin Invest 91:123–32 (1993). 14. Frey M, Packianathan NB, Fehniger TA, et al. Differential expression and function of L-selectin on CD56bright and CD56dim natural killer cell subsets. J Immunol 161:400–8 (1998). 15. Szabo,S.J., Kim,S.T., Costa,G.L., Zhang,X., Fathman,C.G. and Glimcher,L.H. A novel transcription factor, T-bet, directs Th1 lineage commitment Cell 100 (6), 655-669 (2000). 16. Di Santo JP. Natural killer cell developmental pathways: a question of balance. Annu Rev Immunol 24:257–286 (2006). 17. Townsend MJ, et al. T-bet regulates the terminal maturation and homeostasis of NK and Valpha14i NKT cells. Immunity 20:477–494 (2004). 18. Samson SI, et al. GATA-3 promotes maturation, IFN-gamma production, and liverspecific homing of NK cells. Immunity 19:701–711 (2003). 19. Brierley I, Pennell S, Gilbert RJ. Viral RNA pseudoknots: versatile motifs in gene expression and replication. Nat Rev Microbiol 5(8):598-610 (2007) Aug. 20. Moes L, Wirth M. The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codon. Virol J. 22;4:124 (2007) Nov. 21. Ralser M, Querfurth R, Warnatz HJ, Lehrach H, Yaspo ML, Krobitsch S. An efficient and economic enhancer mix for PCR. Biochem Biophys Res Commun. 1;347(3):747-51 (2006) Sep. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26681 | - |
| dc.description.abstract | 在先天免疫系統中，自然殺手細胞扮演一重要的角色，可直接殺死許多癌細胞和被病毒感染的細胞。例如，受到感染的細胞釋放出干擾素活化自然殺手細胞，進而引起一連串的免疫反應殺死受感染的細胞，簡單來說，它們是用來專門摧毀外來的變質細胞。在眾多干擾素中，γ干擾素較為著名，是受到轉錄因子T-bet所調控。T-bet是屬於T-box家族的轉錄因子，能夠於Th1細胞中表達，作為Th1特異的轉錄因子，誘導出γ干擾素的產生，在Th1細胞與自然殺手細胞發育中起著決定性的作用，為了研究自然殺細胞發育的過程，利用microaray 所分析出來的資料去觀察兩種細胞株(NK-92和YT)中的基因表現，我們發現NK-92是類似於CD56bright 自然殺手細胞而YT則是類似於CD56dim自然殺手細胞。同時，也利用real-time PCR的方式去佐證microaray的結果。最後，我們構築突變的T-bet 基因，送入YT細胞株表現，以western blot的呈現，研究T-bet基因的不同的表現量。 | zh_TW |
| dc.description.abstract | Nature killer cells can mediate cellular cytotoxicity against pathogen infected or malignant cells through releasing a wide variety of cytokines, including IFNg, which is controlled by the transcription factor Tbx21/Tbet. T-bet may also regulate the development of Th1 and nature killer cells. To investigate the developmental process of nature killer cells, we analyzed gene expression profiles of two cell lines derived from NK-cell lymphoma, NK-92 and YT, by microarrays. The expression profile suggests that NK-92 was close to CD56bright NK cell and YT is close to CD56dim NK cell. Interestingly, T-bet was weak in the YT cell line despite strong mRNA expression. Real-time PCR and western blot were performed for confirmation and for dissecting the regulation of T-bet expression. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:20:37Z (GMT). No. of bitstreams: 1 ntu-97-R95444004-1.pdf: 784400 bytes, checksum: 5ba7a7a602163a2e26a3a54eda71cbf4 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 誌謝………………………………………………………………………3
中文摘要………………………………………………………………..5 Abstract…………………………………………………………………6 Introduction……..........................................7 Materials and Methods………………………………………………10 Results…………………………………………………………………20 Discussion….............................................24 References…………………………………………………………….26 Tables………………………………………………………………….29 Figures…………………………………………………………………31 Appendix……………………………………………………………….37 | |
| dc.language.iso | en | |
| dc.subject | 自然殺手細胞 | zh_TW |
| dc.subject | 微陣列 | zh_TW |
| dc.subject | 轉錄因子 | zh_TW |
| dc.subject | 干擾素 | zh_TW |
| dc.subject | T-bet | en |
| dc.subject | pseudoknot | en |
| dc.subject | microarray | en |
| dc.subject | IFNg | en |
| dc.title | YT和NK-92細胞株之轉錄因子與細胞激素的後轉錄調控 | zh_TW |
| dc.title | Microarray on YT & NK 92 lymphoma cell lines of NK-cell origin: Post-transcriptional regulation of cytokines & their transcriptional factors | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林欽塘,周綠蘋,吳漢忠 | |
| dc.subject.keyword | 轉錄因子,干擾素,微陣列,自然殺手細胞, | zh_TW |
| dc.subject.keyword | T-bet,IFNg,microarray,pseudoknot, | en |
| dc.relation.page | 61 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2008-07-25 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 病理學研究所 | zh_TW |
| Appears in Collections: | 病理學科所 | |
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| File | Size | Format | |
|---|---|---|---|
| ntu-97-1.pdf Restricted Access | 766.02 kB | Adobe PDF |
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