阿拉伯芥中 Osmotin 同源蛋白質的的選殖與生理研究

Abstract

Osmotin from tobacco can induce program cell death (PCD) of yeast by binding to a yeast membrane protein, YOL002c, which is a homolog of mammalian adiponectin receptor . Tobacco osmotin shares a similar structure fold with mammalian adiponectin with no sequence similarity. HHP1 (UhUeptaUhUelical UpUrotein 1) is a homolog of adiponectin receptor in Arabidopsis with 51% sequence similarity. It is interesting to find out if there is any physiological or biochemical interaction between AtOSM34 and HHP1 of Arabidopsis. AtOSM34 is a homolog of tobacco osmotin in Arabidopsis. Heterologous protein expression of AtOSM34 in E.coli was established. Cellular localization of AtOSM34 was studied using transient expression of AtOSM34-mGFP5 fusion protein in onion epidermal cells and Aradidopsis protoplasts. The plasmid used for AtOSM34-mGFP5 fusion protein expression was constructed using pCAMBIA1302 vector. It is likely that AtOSM34 is located in the cytosol or other subcellular organelle. We also tried to treat Arabidopsis protoplasts using recombinant AtOSM34. The possibility of a putative receptor for AtOSM34 is discussed.