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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
dc.contributor.author | Pei-Chun Liu | en |
dc.contributor.author | 劉佩群 | zh_TW |
dc.date.accessioned | 2021-06-08T07:11:08Z | - |
dc.date.copyright | 2008-08-06 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-31 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26461 | - |
dc.description.abstract | 本研究利用微機電技術製作三極式電化學微感測電極監測生理參數Glucose、Lactate,並針對微小化參考電極需具穩定性、可再現性、可靠性之需求,以及考量植入式感測器可能面臨之問題,提出藉由覆蓋於感測器上之保護膜(Protective membrane)來改善,期望除了能提高感測器之生物相容性或耐久性外,進而可同時提高微小化參考電極的穩定性。此外,為了將檢測系統即電化學感測系統之架構簡化與微小化,本研究也設計出單電源(+3V)定電壓之電化學感測電路,達到最終微小化之目的。
觀察不同保護膜材料對參考電極之穩定性探討,由結果顯示Polyurethane(PU)保護膜確實能改善參考電極之穩定性,因此後續實驗以Polyurethane(PU)保護膜為主,觀察其對酵素電極之感測影響,結果發現具Polyurethane(PU)保護膜之Glucose biosensor或Lactate biosensor並不會影響其感測能力,且動物實驗中得知植入數天後具Polyurethane(PU)保護膜之Glucose biosensor或Lactate biosensor同樣不會影響其感測能力!而觀察植入後之感測器電極表面,發現所有感測器植入數天後電極表面之Polyurethane(PU)保護膜結構皆無破壞,仍具保護功能! | zh_TW |
dc.description.abstract | In our study, preparation of the three-electrode biosensors used the MEMS technology. The three-electrode biosensors measured glucose and lactate using glucose oxidase (GOD) and lactate dehydrogenase (LDH). Biomedical and implantable sensor applications require stable, reproducible, reversible, and reliable miniaturized reference electrode , made of biocompatible materials. So the present study reports the application of protective membrane on the device, which exists both biocompatibility and permselectivity, and acts as a surface coating material for the outer layer of the Bio-sensor. For miniaturization of sensing system, we design a single-supply circuit.
Thus, our study used difference protective membrane to assure the long-term stability of the Ag/AgCl reference electrode. From those results, we can know that reference electrode with polyurethane protective membrane is more stable than the one without protective membrane. In vivo performance, the sensor with polyurethane protective membrane can also work when implanted subcutaneously in a rat. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:11:08Z (GMT). No. of bitstreams: 1 ntu-97-R95921044-1.pdf: 4133636 bytes, checksum: f86838718d87fc313921ae92bcd2aac5 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iii 英文摘要 iv 目錄 v 圖目錄 vii 表目錄 xi 第一章 緒論 1 1-1 前言 1 1-2 研究背景 2 1-2-1文獻回顧 2 1-2-2生理參數之選擇 7 1-3 研究動機與目的 9 1-4 論文之架構 11 第二章 實驗理論基礎 12 2-1 生物感測器 12 2-2 電化學生物感測器 17 2-2-1 何謂電化學 17 2-2-2 電化學反應種類 18 2-2-3 電極與溶液介面關係 21 2-2-4 電化學分析方法 22 第三章 實驗方法 25 3-1 三極式電化學微感測電極之研製 25 3-1-1 三極式電化學微感測電極之規格 25 3-1-2 微機電技術製作三極式電極之流程與方法 26 3-1-3 微機電技術製作三極式電極之製程技術 28 3-1-3-1 晶片清洗 28 3-1-3-2 光微影術 28 3-1-3-3 物理薄膜沉積 32 3-1-3-4 光阻剝離法 33 3-1-3-5 電鍍RE 34 3-2 電化學感測電極之修飾與感測 35 3-2-1 GOD、LDH感測機制 35 3-2-2 Polymer包埋固定酵素 37 3-2-3 保護膜(Protective membrane)之製作 39 3-2-3-1 Nafion保護膜之製作 39 3-2-3-2 Polyurethane保護膜之製作 41 3-2-4 實驗藥品與儀器 44 3-3 量測平台微小化之建立 47 3-3-1 電化學電路設計概念 48 3-3-2 單電源定電壓電化學感測電路設計 52 第四章 動物實驗測試 54 4-1 植入生物體之實驗步驟(Implantation) 54 4-2 In vivo量測與生物相容性評估 60 第五章 實驗結果與討論 61 5-1 三極式電化學微感測電極之研製 61 5-1-1 三極式電化學微感測電極完成實體 61 5-1-2 三極式電化學微感測電極功能性驗證 64 5-2 電化學微感測電極之之修飾與感測 66 5-2-1 保護膜對參考電極( Reference Electrode)之穩定性探討 66 5-2-2 Polyurethane(PU)對酵素電極之測試 69 5-2-3 Polyurethane(PU)對酵素電極之穩定性探討 72 5-3 量測平台微小化之建立 73 5-4 動物實驗之測試 77 5-4-1功能性測試 77 5-4-1-1 葡萄糖氧化酶(Glucose Oxidase,GOD) 功能測試 79 5-4-1-2 乳酸脫氫酵素 ( L-Lactic Dehydrogenase,LDH) 功能測試 81 5-4-2 生物相容性評估 84 第六章 結論與未來展望 92 6-1 結論 92 6-2 未來展望 93 參考文獻 95 | |
dc.language.iso | zh-TW | |
dc.title | 具高分子保護膜之植入式微型化酵素感測器研製 | zh_TW |
dc.title | Development of Implantable Enzyme Biosensors by Using Biomedical Polymer Protective Membrane | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林致廷,楊燿州,楊龍杰 | |
dc.subject.keyword | 微機電技術,植入式,酵素感測器,乳酸脫氫酵素,葡萄糖氧化酵素,聚氨酯, | zh_TW |
dc.subject.keyword | Micro-Electro-Mechanical System,Implant,Enzyme biosensor,Lactate dehydrogenase,Glucose Oxidase,Polyurethane membrane, | en |
dc.relation.page | 99 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2008-07-31 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 電機工程學研究所 | zh_TW |
顯示於系所單位: | 電機工程學系 |
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