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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃青真(Ching-jang Huang) | |
| dc.contributor.author | Meng-fan Lu | en |
| dc.contributor.author | 呂孟凡 | zh_TW |
| dc.date.accessioned | 2021-06-08T07:06:34Z | - |
| dc.date.copyright | 2011-08-22 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-12 | |
| dc.identifier.citation | 王敦正（2002）。乳癌磷酸酶(Brk)與HaCaT細胞分化關係之研究。國立台灣大學分子醫學研究所碩士論文，未出版，台北市。
徐瑨（2011）。自山苦瓜單離植物雌激素及其化學鑑定與生物活性探討。國立台灣大學生化科技學系博士論文，未出版，台北市。 Adeline, F. D. & Van, G. ( 2007) WilsonIn vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line. Cytotechnology 54, 77–83. Agrawal, R.C., Beohar, T. (2010) Chemopreventive and Anticarcinogenic Effects of Momordica Charantia Extract. Asian Pacific J Cancer Prev 11, 371-375. Ameen, M., Exarchou, V., Chu, A.C. (2001) Topical calcipotriol as monotherapy and in combination with psoralen plus ultraviolet A in the treatment of vitiligo. Br J Dermatol 145: 476-9. Ames, B.N., Shigenaga, M.K., Hagen, T.M. (1993) Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A 90: 7915-22. Balazs, I.T., Olah, A., Attila G.S.,Czifra, G., and Biro, T. (2011) “Sebocytes’ makeup” - Novel mechanisms and concepts in the physiology of the human sebaceous glands. Eur J Physiol 461:593–606. Basch, E., Gabardi, S., and Ulbricht, C. (2003). 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26330 | - |
| dc.description.abstract | 皮膚是人體抵禦外來侵襲的第一道防線，其中角質細胞與皮脂細胞對滲透性屏障功能扮演著重要角色。許多研究指出，活化PPAR可促進角質細胞分化及皮脂細胞合成脂質，具有促進皮膚保健之功效；而本實驗室先前的研究結果顯示山苦瓜乙酸乙酯萃物可活化PPAR-alpha與PPAR-gamma。因此本研究的目的為：以人類角質細胞株HaCaT以及皮脂細胞株SZ95，探討山苦瓜萃物對角質細胞增生與分化以及皮脂細胞脂質生成的影響。
將HaCaT和SZ95細胞以山苦瓜乙酸乙酯萃物、皂化物及不皂化物處理後，以MTT偵測HaCaT細胞增生，並以Q-PCR偵測HaCaT細胞內四種分化指標基因keratin 1 (K1)、kerain 10 (K10)、involucrin與transglutaminase-1（TGM1）以及SZ95皮脂細胞分化指標基因melanocortin-5 receptor (MC5R)之表現。另以油紅染色分析皮脂細胞脂質生成。 實驗結果顯示，山苦瓜乙酸乙酯萃物與其皂化物可促進HaCaT細胞之增生；而山苦瓜乙酸乙酯萃物暨其區分物皆會促進K1、K10與involucrin之表現，此外乙酸乙酯萃物與不皂化物還會促進TGM1表現。山苦瓜乙酸乙酯萃物暨其區分物皆會促進SZ95皮脂細胞脂質生成；皂化物與不皂化物會促進SZ95皮脂細胞MC5R表現。 以上結果顯示，山苦瓜乙酸乙酯萃物暨其區分物具有促進角質細胞增生與分化以及皮脂細胞脂質生成的效果，推測可能具有促進滲透性屏障功能之潛力，具皮膚保健之功效。 | zh_TW |
| dc.description.abstract | Skin is the first line of human body defense against external invasion. Both keratinocyte and sebocyte play an important role in the permeability barrier function of skin. Many literatures showed that PPAR activation induced keratinocyte differentiation and sebocyte lipogenesis. Previous findings in our laboratory revealed that bitter melon ethyl acetate (EA) extract activate PPAR-alpha and PPAR-gamma | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T07:06:34Z (GMT). No. of bitstreams: 1 ntu-100-R98b47301-1.pdf: 6497356 bytes, checksum: d734c029c09a84a10d5acd2bc51e3f7b (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 總目錄
口試委員審定書 i 謝誌 ii 中文摘要 iv Abstract v 總目錄 I 表目錄 IV 圖目錄 V 第一章 緒論 1 第一節 研究動機與目的 1 第二節 文獻回顧 2 一、 皮膚的結構 2 (一) 表皮層 (epidermis) 3 (二) 真皮層（dermis） 4 (三) 皮下組織（hypodermis） 8 二、 滲透性屏障 （Permeability barrier） 8 三、 過氧化體增生器活化受器（PPARs） 11 (一) PPAR種類及作用機制 11 (二) PPAR與皮膚的相關性 12 四、 山苦瓜 17 第三節 實驗假說與實驗設計 21 一、 研究假說 21 二、 實驗架構 21 第二章 山苦瓜樣品對人類角質細胞株HaCaT生長與分化之影響 22 第一節 前言 22 第二節 材料與方法 23 一、 細胞株 23 二、 試劑 24 (一) 細胞培養 24 (二) H&E staining 25 (三) 定量聚合酶連鎖反應(Quantitative-Polymerase Chain Reaction, Q-PCR) 25 三、 儀器設備 26 四、 實驗材料 27 五、 實驗方法 28 (一) HaCaT 細胞培養 28 (二) HaCaT cell proliferation assay 29 (三) HaCaT cell differentiation assay 30 (四) HaCaT cell morphology 32 六、 數據整理與統計分析 33 第三節 結果 34 第四節 討論 56 第五節 結論 60 第三章 山苦瓜樣品對人類皮脂腺細胞SZ95皮脂含量及分化之影響 61 第一節 前言 61 第二節 材料與方法 62 一、 細胞株 62 二、 試劑 63 (一) 細胞培養 63 (二) 皮脂細胞油紅染色用試劑（細胞觀察及脂質定量） 64 (三) 定量聚合酶連鎖反應(Quantitative-Polymerase Chain Reaction, Q-PCR) 64 三、 儀器設備 65 四、 實驗材料 65 五、 實驗方法 66 (一) SZ95 皮脂細胞培養 66 (二) SZ95 sebocyte chamber slide 67 (三) SZ95 sebocyte lipid content assay 68 (四) SZ95 sebocyte differentiation assay 69 六、 數據整理與統計分析 69 第三節 結果 70 第四節 討論 80 第五節 結論 82 第四章 綜合討論與總結論 83 第一節 綜合討論 83 第二節 總結論 85 | |
| dc.language.iso | zh-TW | |
| dc.title | 山苦瓜萃物對人類角質細胞及皮脂細胞之增生與分化的影響 | zh_TW |
| dc.title | In vitro effects of Momrdica charantia L. extracts on the growth and differentiation of skin cells: HaCaT keratinocyte and SZ 95 sebocyte | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 蔡帛蓉(Po-Jung Tsai) | |
| dc.contributor.oralexamcommittee | 林璧鳳,吳文惠,蔡宗憲 | |
| dc.subject.keyword | 皮膚,滲透性屏障功能,HaCaT細胞,SZ95皮脂細胞,山苦瓜, | zh_TW |
| dc.subject.keyword | skin,permeability barrier function,HaCaT cell,SZ95sebocyt,wild bitter melon, | en |
| dc.relation.page | 95 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-08-12 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-100-1.pdf 目前未授權公開取用 | 6.35 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。