請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26219
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 周子賓 | |
dc.contributor.author | Yi-Min Chen | en |
dc.contributor.author | 陳奕閔 | zh_TW |
dc.date.accessioned | 2021-06-08T07:03:13Z | - |
dc.date.copyright | 2011-08-16 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-15 | |
dc.identifier.citation | Aizer A, Brody Y, Ler LW, Sonenberg N, Singer RH, Shav-Tal Y (2008) The Dynamics of Mammalian P Body Transport, Assembly, and Disassembly In Vivo. Molecular Biology of the Cell 19: 4154-4166
Akhmanova A, Steinmetz MO (2008) Tracking the ends: a dynamic protein network controls the fate of microtubule tips. Nat Rev Mol Cell Biol 9: 309-322 Al-Bassam J, Ozer RS, Safer D, Halpain S, Milligan RA (2002) MAP2 and tau bind longitudinally along the outer ridges of microtubule protofilaments. J Cell Biol 157: 1187-1196 Andrei MA, Ingelfinger D, Heintzmann R, Achsel T, Rivera-Pomar R, Luhrmann R (2005) A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies. RNA 11: 717-727 Arnaoutov A, Azuma Y, Ribbeck K, Joseph J, Boyarchuk Y, Karpova T, McNally J, Dasso M (2005) Crm1 is a mitotic effector of Ran-GTP in somatic cells. Nat Cell Biol 7: 626-632 Babu K (2004) Roles of Bifocal, Homer, and F-actin in anchoring Oskar to the posterior cortex of Drosophila oocytes. Genes & Development 18: 138-143 Babu K, Cai Y, Bahri S, Yang X, Chia W (2004) Roles of Bifocal, Homer, and F-actin in anchoring Oskar to the posterior cortex of Drosophila oocytes. Genes Dev 18: 138-143 Baum B (2002) Winging it--actin on the fly. Dev Cell 2: 125-126 Becalska AN, Gavis ER (2010) Bazooka regulates microtubule organization and spatial restriction of germ plasm assembly in the Drosophila oocyte. Dev Biol 340: 528-538 Bessman MJ, Frick DN, O'Handley SF (1996) The MutT proteins or 'Nudix' hydrolases, a family of versatile, widely distributed, 'housecleaning' enzymes. J Biol Chem 271: 25059-25062 Bohrmann J, Biber K (1994) Cytoskeleton-dependent transport of cytoplasmic particles in previtellogenic to mid-vitellogenic ovarian follicles of Drosophila: time-lapse analysis using video-enhanced contrast microscopy. J Cell Sci 107 ( Pt 4): 849-858 Breitwieser W, Markussen FH, Horstmann H, Ephrussi A (1996) Oskar protein interaction with Vasa represents an essential step in polar granule assembly. Genes Dev 10: 2179-2188 Brendza RP, Serbus LR, Duffy JB, Saxton WM (2000) A function for kinesin I in the posterior transport of oskar mRNA and Staufen protein. Science 289: 2120-2122 Busson D, Gans M, Komitopoulou K, Masson M (1983) Genetic Analysis of Three Dominant Female-Sterile Mutations Located on the X Chromosome of DROSOPHILA MELANOGASTER. Genetics 105: 309-325 Cha BJ, Koppetsch BS, Theurkauf WE (2001) In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway. Cell 106: 35-46 Cha BJ, Serbus LR, Koppetsch BS, Theurkauf WE (2002) Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posterior. Nat Cell Biol 4: 592-598 Cho PF, Poulin F, Cho-Park YA, Cho-Park IB, Chicoine JD, Lasko P, Sonenberg N (2005) A new paradigm for translational control: inhibition via 5'-3' mRNA tethering by Bicoid and the eIF4E cognate 4EHP. Cell 121: 411-423 Chou TB, Perrimon N (1992) Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila. Genetics 131: 643-653 Chou TB, Perrimon N (1996) The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster. Genetics 144: 1673-1679 Clark A, Meignin C, Davis I (2007) A Dynein-dependent shortcut rapidly delivers axis determination transcripts into the Drosophila oocyte. Development 134: 1955-1965 Clark I, Giniger E, Ruohola-Baker H, Jan LY, Jan YN (1994) Transient posterior localization of a kinesin fusion protein reflects anteroposterior polarity of the Drosophila oocyte. Curr Biol 4: 289-300 Coller J, Parker R (2005) General translational repression by activators of mRNA decapping. Cell 122: 875-886 Cooley L, Theurkauf WE (1994) Cytoskeletal functions during Drosophila oogenesis. Science 266: 590-596 Cougot N, van Dijk E, Babajko S, Seraphin B (2004) 'Cap-tabolism'. Trends Biochem Sci 29: 436-444 Cox MM (1983) The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli. Proc Natl Acad Sci U S A 80: 4223-4227 Dahlgaard K, Raposo AA, Niccoli T, St Johnston D (2007) Capu and Spire assemble a cytoplasmic actin mesh that maintains microtubule organization in the Drosophila oocyte. Dev Cell 13: 539-553 Decker CJ, Teixeira D, Parker R (2007) Edc3p and a glutamine/asparagine-rich domain of Lsm4p function in processing body assembly in Saccharomyces cerevisiae. J Cell Biol 179: 437-449 Dhadialla ASRaTS (1992) accumulation of yolk proteins in insect oocytes. Annu Rev Entomol Ding D, Parkhurst SM, Halsell SR, Lipshitz HD (1993) Dynamic Hsp83 RNA localization during Drosophila oogenesis and embryogenesis. Mol Cell Biol 13: 3773-3781 Doerflinger H, Benton R, Shulman JM, St Johnston D (2003) The role of PAR-1 in regulating the polarised microtubule cytoskeleton in the Drosophila follicular epithelium. Development 130: 3965-3975 Drewes G, Ebneth A, Mandelkow EM (1998) MAPs, MARKs and microtubule dynamics. Trends Biochem Sci 23: 307-311 Drewes G, Ebneth A, Preuss U, Mandelkow EM, Mandelkow E (1997) MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption. Cell 89: 297-308 Driever W, Nusslein-Volhard C (1989) The bicoid protein is a positive regulator of hunchback transcription in the early Drosophila embryo. Nature 337: 138-143 Dye RB, Fink SP, Williams RC, Jr. (1993) Taxol-induced flexibility of microtubules and its reversal by MAP-2 and Tau. J Biol Chem 268: 6847-6850 Ebneth A, Drewes G, Mandelkow EM, Mandelkow E (1999) Phosphorylation of MAP2c and MAP4 by MARK kinases leads to the destabilization of microtubules in cells. Cell Motil Cytoskeleton 44: 209-224 Emmons S, Phan H, Calley J, Chen W, James B, Manseau L (1995) Cappuccino, a Drosophila maternal effect gene required for polarity of the egg and embryo, is related to the vertebrate limb deformity locus. Genes Dev 9: 2482-2494 Ephrussi A, Dickinson LK, Lehmann R (1991) Oskar organizes the germ plasm and directs localization of the posterior determinant nanos. Cell 66: 37-50 Ephrussi A, Lehmann R (1992) Induction of germ cell formation by oskar. Nature 358: 387-392 Ephrussi A, St Johnston D (2004) Seeing is believing: the bicoid morphogen gradient matures. Cell 116: 143-152 Erdelyi M, Michon AM, Guichet A, Glotzer JB, Ephrussi A (1995) Requirement for Drosophila cytoplasmic tropomyosin in oskar mRNA localization. Nature 377: 524-527 Eulalio A, Behm-Ansmant I, Izaurralde E (2007a) P bodies: at the crossroads of post-transcriptional pathways. Nat Rev Mol Cell Biol 8: 9-22 Eulalio A, Behm-Ansmant I, Schweizer D, Izaurralde E (2007b) P-Body Formation Is a Consequence, Not the Cause, of RNA-Mediated Gene Silencing. Molecular and Cellular Biology 27: 3970-3981 Fan SJ, Marchand V, Ephrussi A (2011) Drosophila Ge-1 Promotes P Body Formation and oskar mRNA Localization. PLoS One 6: e20612 Fenger-Grøn M, Fillman C, Norrild B, Lykke-Andersen J (2005) Multiple Processing Body Factors and the ARE Binding Protein TTP Activate mRNA Decapping. Molecular Cell 20: 905-915 Ferraiuolo MA, Basak S, Dostie J, Murray EL, Schoenberg DR, Sonenberg N (2005) A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay. J Cell Biol 170: 913-924 Forrest KM, Gavis ER (2003) Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr Biol 13: 1159-1168 Franks TM, Lykke-Andersen J (2008a) The control of mRNA decapping and P-body formation. Mol Cell 32: 605-615 Franks TM, Lykke-Andersen J (2008b) The Control of mRNA Decapping and P-Body Formation. Molecular Cell 32: 605-615 Glotzer JB, Saffrich R, Glotzer M, Ephrussi A (1997) Cytoplasmic flows localize injected oskar RNA in Drosophila oocytes. Curr Biol 7: 326-337 Golic KG, Lindquist S (1989) The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59: 499-509 Henrique D, Schweisguth F (2003) Cell polarity: the ups and downs of the Par6/aPKC complex. Curr Opin Genet Dev 13: 341-350 Idriss HT (2000a) Man to trypanosome: the tubulin tyrosination/detyrosination cycle revisited. Cell Motil Cytoskeleton 45: 173-184 Idriss HT (2000b) Phosphorylation of tubulin tyrosine ligase: a potential mechanism for regulation of alpha-tubulin tyrosination. Cell Motil Cytoskeleton 46: 1-5 Interthal H, Bellocq C, Bahler J, Bashkirov VI, Edelstein S, Heyer WD (1995) A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae. EMBO J 14: 1057-1066 Jankovics F, Sinka R, Lukacsovich T, Erdelyi M (2002) MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. Curr Biol 12: 2060-2065 Januschke J, Gervais L, Dass S, Kaltschmidt JA, Lopez-Schier H, St Johnston D, Brand AH, Roth S, Guichet A (2002) Polar transport in the Drosophila oocyte requires Dynein and Kinesin I cooperation. Curr Biol 12: 1971-1981 Jinek M, Eulalio A, Lingel A, Helms S, Conti E, Izaurralde E (2008) The C-terminal region of Ge-1 presents conserved structural features required for P-body localization. RNA 14: 1991-1998 Johnston SC, Zhang H, Messina LM, Lawton MT, Dean D (2005) Chlamydia pneumoniae burden in carotid arteries is associated with upregulation of plaque interleukin-6 and elevated C-reactive protein in serum. Arterioscler Thromb Vasc Biol 25: 2648-2653 Johnstone O, Lasko P (2001) Translational regulation and RNA localization in Drosophila oocytes and embryos. Annu Rev Genet 35: 365-406 Joly JC, Flynn G, Purich DL (1989) The microtubule-binding fragment of microtubule-associated protein-2: location of the protease-accessible site and identification of an assembly-promoting peptide. J Cell Biol 109: 2289-2294 Kamal A, Goldstein LS (2002) Principles of cargo attachment to cytoplasmic motor proteins. Curr Opin Cell Biol 14: 63-68 Kedersha N, Anderson P (2007) Mammalian stress granules and processing bodies. Methods Enzymol 431: 61-81 Kim-Ha J, Smith JL, Macdonald PM (1991) oskar mRNA is localized to the posterior pole of the Drosophila oocyte. Cell 66: 23-35 Krichevsky AM, Kosik KS (2001) Neuronal RNA granules: a link between RNA localization and stimulation-dependent translation. Neuron 32: 683-696 Lehmann R, Nusslein-Volhard C (1986) Abdominal segmentation, pole cell formation, and embryonic polarity require the localized activity of oskar, a maternal gene in Drosophila. Cell 47: 141-152 Li R, Gundersen GG (2008) Beyond polymer polarity: how the cytoskeleton builds a polarized cell. Nature Reviews Molecular Cell Biology 9: 860-873 Lin M-D, Fan S-J, Hsu W-S, Chou T-B (2006) Drosophila Decapping Protein 1, dDcp1, Is a Component of the oskar mRNP Complex and Directs Its Posterior Localization in the Oocyte. Developmental Cell 10: 601-613 Ling SH, Decker CJ, Walsh MA, She M, Parker R, Song H (2008) Crystal structure of human Edc3 and its functional implications. Mol Cell Biol 28: 5965-5976 Ling SHM, Qamra R, Song H (2011) Structural and functional insights into eukaryotic mRNA decapping. Wiley Interdisciplinary Reviews: RNA 2: 193-208 Liu J, Rivas FV, Wohlschlegel J, Yates JR, 3rd, Parker R, Hannon GJ (2005) A role for the P-body component GW182 in microRNA function. Nat Cell Biol 7: 1261-1266 Luby-Phelps K (2000) Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area. Int Rev Cytol 192: 189-221 Mallardo M, Deitinghoff A, Muller J, Goetze B, Macchi P, Peters C, Kiebler MA (2003) Isolation and characterization of Staufen-containing ribonucleoprotein particles from rat brain. Proc Natl Acad Sci U S A 100: 2100-2105 Manseau L, Calley J, Phan H (1996) Profilin is required for posterior patterning of the Drosophila oocyte. Development 122: 2109-2116 Markussen FH, Michon AM, Breitwieser W, Ephrussi A (1995) Translational control of oskar generates short OSK, the isoform that induces pole plasma assembly. Development 121: 3723-3732 Messitt TJ, Gagnon JA, Kreiling JA, Pratt CA, Yoon YJ, Mowry KL (2008) Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes. Dev Cell 15: 426-436 Minshall N, Standart N (2004) The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer. Nucleic Acids Res 32: 1325-1334 Minshall N, Thom G, Standart N (2001) A conserved role of a DEAD box helicase in mRNA masking. RNA 7: 1728-1742 Mitchison T, Kirschner M (1984) Dynamic instability of microtubule growth. Nature 312: 237-242 Mizuno N, Toba S, Edamatsu M, Watai-Nishii J, Hirokawa N, Toyoshima YY, Kikkawa M (2004) Dynein and kinesin share an overlapping microtubule-binding site. EMBO J 23: 2459-2467 Mlodzik M, Gehring WJ (1987) Expression of the caudal gene in the germ line of Drosophila: formation of an RNA and protein gradient during early embryogenesis. Cell 48: 465-478 Muhlrad D, Decker CJ, Parker R (1994) Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript. Genes Dev 8: 855-866 Nakamura A, Amikura R, Hanyu K, Kobayashi S (2001) Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis. Development 128: 3233-3242 Nance J, Zallen JA (2011) Elaborating polarity: PAR proteins and the cytoskeleton. Development 138: 799-809 Navarro RE, Shim EY, Kohara Y, Singson A, Blackwell TK (2001) cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans. Development 128: 3221-3232 Neuman-Silberberg FS, Schupbach T (1993) The Drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGF alpha-like protein. Cell 75: 165-174 Ohashi S, Koike K, Omori A, Ichinose S, Ohara S, Kobayashi S, Sato TA, Anzai K (2002) Identification of mRNA/protein (mRNP) complexes containing Puralpha, mStaufen, fragile X protein, and myosin Va and their association with rough endoplasmic reticulum equipped with a kinesin motor. J Biol Chem 277: 37804-37810 Palacios IM, St Johnston D (2002) Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte. Development 129: 5473-5485 Parker R, Sheth U (2007) P bodies and the control of mRNA translation and degradation. Mol Cell 25: 635-646 Payre F, Crozatier M, Vincent A (1994) Direct control of transcription of the Drosophila morphogen bicoid by the serendipity delta zinc finger protein, as revealed by in vivo analysis of a finger swap. Genes Dev 8: 2718-2728 Perrimon N (1984) Clonal Analysis of Dominant Female-Sterile, Germline-Dependent Mutations in DROSOPHILA MELANOGASTER. Genetics 108: 927-939 Piccirillo C, Khanna R, Kiledjian M (2003) Functional characterization of the mammalian mRNA decapping enzyme hDcp2. RNA 9: 1138-1147 Pignoni F, Zipursky SL (1997) Induction of Drosophila eye development by decapentaplegic. Development 124: 271-278 Pilkington GR, Parker R (2008) Pat1 contains distinct functional domains that promote P-body assembly and activation of decapping. Mol Cell Biol 28: 1298-1312 Reijns MA, Alexander RD, Spiller MP, Beggs JD (2008) A role for Q/N-rich aggregation-prone regions in P-body localization. J Cell Sci 121: 2463-2472 Riechmann V, Ephrussi A (2004) Par-1 regulates bicoid mRNA localisation by phosphorylating Exuperantia. Development 131: 5897-5907 Rodriguez AJ, Seipel SA, Hamill DR, Romancino DP, M DIC, Suprenant KA, Bonder EM (2005) Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes. RNA 11: 646-656 Rorth P (1998) Gal4 in the Drosophila female germline. Mech Dev 78: 113-118 Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218: 348-353 Schneider MD, Najand N, Chaker S, Pare JM, Haskins J, Hughes SC, Hobman TC, Locke J, Simmonds AJ (2006) Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila development. J Cell Biol 174: 349-358 Schupbach T (1987) Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster. Cell 49: 699-707 Serbus LR (2005) Dynein and the actin cytoskeleton control kinesin-driven cytoplasmic streaming in Drosophila oocytes. Development 132: 3743-3752 She M, Decker CJ, Chen N, Tumati S, Parker R, Song H (2006) Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe. Nat Struct Mol Biol 13: 63-70 She M, Decker CJ, Svergun DI, Round A, Chen N, Muhlrad D, Parker R, Song H (2008) Structural basis of dcp2 recognition and activation by dcp1. Mol Cell 29: 337-349 Shulman JM, Benton R, St Johnston D (2000) The Drosophila homolog of C. elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole. Cell 101: 377-388 Solinger JA, Pascolini D, Heyer WD (1999) Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis. Mol Cell Biol 19: 5930-5942 Steiger M, Carr-Schmid A, Schwartz DC, Kiledjian M, Parker R (2003) Analysis of recombinant yeast decapping enzyme. RNA 9: 231-238 Sweet TJ, Boyer B, Hu W, Baker KE, Coller J (2007) Microtubule disruption stimulates P-body formation. RNA 13: 493-502 Teixeira D, Parker R (2007) Analysis of P-body assembly in Saccharomyces cerevisiae. Mol Biol Cell 18: 2274-2287 Tharun S, He W, Mayes AE, Lennertz P, Beggs JD, Parker R (2000) Yeast Sm-like proteins function in mRNA decapping and decay. Nature 404: 515-518 Tharun S, Parker R (2001) Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs. Mol Cell 8: 1075-1083 Theurkauf WE, Smiley S, Wong ML, Alberts BM (1992) Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport. Development 115: 923-936 Tomancak P, Piano F, Riechmann V, Gunsalus KC, Kemphues KJ, Ephrussi A (2000) A Drosophila melanogaster homologue of Caenorhabditis elegans par-1 acts at an early step in embryonic-axis formation. Nat Cell Biol 2: 458-460 Tritschler F, Eulalio A, Truffault V, Hartmann MD, Helms S, Schmidt S, Coles M, Izaurralde E, Weichenrieder O (2007) A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body targeting. Mol Cell Biol 27: 8600-8611 Vanzo N, Oprins A, Xanthakis D, Ephrussi A, Rabouille C (2007) Stimulation of Endocytosis and Actin Dynamics by Oskar Polarizes the Drosophila Oocyte. Developmental Cell 12: 543-555 Vanzo NF, Ephrussi A (2002) Oskar anchoring restricts pole plasm formation to the posterior of the Drosophila oocyte. Development 129: 3705-3714 Watanabe N, Higashida C (2004) Formins: processive cappers of growing actin filaments. Exp Cell Res 301: 16-22 Wodarz A (2002) Establishing cell polarity in development. Nat Cell Biol 4: E39-44 Xu J, Yang JY, Niu QW, Chua NH (2006) Arabidopsis DCP2, DCP1, and VARICOSE form a decapping complex required for postembryonic development. Plant Cell 18: 3386-3398 Yu JH (2005) Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body. Rna 11: 1795-1802 Zhai Y, Kronebusch PJ, Simon PM, Borisy GG (1996) Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis. J Cell Biol 135: 201-214 Zhang HL, Eom T, Oleynikov Y, Shenoy SM, Liebelt DA, Dictenberg JB, Singer RH, Bassell GJ (2001) Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility. Neuron 31: 261-275 Zimyanin VL, Belaya K, Pecreaux J, Gilchrist MJ, Clark A, Davis I, St Johnston D (2008) In vivo imaging of oskar mRNA transport reveals the mechanism of posterior localization. Cell 134: 843-853 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26219 | - |
dc.description.abstract | 在果蠅卵子發育(oogenesis)時期,母源訊息核醣核酸(maternal mRNA), 如oskar mRNA,坐落在卵母細胞(oocyte)的後端並決定胚胎發育的軸向(axial determination)以及極細胞(pole cell)的發育。細胞內的微管(microtubule) 和纖維性機動蛋白(F-actin) 參與oskar mRNA的運送及卵子後端(posterior pole)的定位。
本論文研究人類促進去頭蓋大分子在果蠅中的同源基因 Drosophila Human enhancer of decapping large subunit (dHedls), 又稱為dGe-1(Gougerot syndrome)。 dGe-1在生殖細胞的缺失(dGe-1 loss-of-function in germline),會影響卵母細胞內微管 A-P gradient和bundles的形成;同時也會使後期的細胞質流(ooplasmic streaming)有停滯的現象。 dGe-1突變的背景下,組成微管結構的單體微管蛋白(α-tubulin)和果蠅去頭蓋蛋白質2,dDcp2的表現量都會下降;且在此背景下,大量表現dDcp2可以抑制dGe-1缺失所造成的卵母細胞內微管失序的性狀;我們推論dGe-1和 dDcp2在微管調控上有複合體的關係。 經酵母菌雙雜交系統(yeast-two hybrid system)也證實 dGe-1和dDcp2彼此之間有直接的交互作用(physical interaction)。免疫螢光染色也觀察到dDcp2和dGe-1坐落在果蠅卵母細胞皮層處(oocyte cortex),並有局部疊合(partial coloclaized)的現象。 因此我們推論dGe-1和dDcp2彼此之間會在卵母細胞皮層處有直接的交互作用,也共同調控細胞微管的組成, oskar mRNA在卵母細胞的運送及後端定位進而被影響。 | zh_TW |
dc.description.abstract | In Drosophila oogenesis, the localization of the maternal mRNAs at specific positions, such as oskar mRNA, is an important mechanism, which regulates axial determination and pole cell development in embryogenesis. In Drosophila oocyte, the location and anchoring of oskar mRNA at posterior pole are regulated by F-actin and microtubules.First, in my study, the Drosophila Human enhancer of decapping large subunit, (dHedls), which also called dGe-1(Gougerot syndrome) mutant GLC displays microtubule disrupted phenotypes, which are A-P gradient lost in stage 9 oocytes and microtubules bundles lost in stage 10. Ooplasmic streaming is also obstructed.
α-tubulin and Drosophila decapping protein 2 (dDcp2) protein expression levels decreased in dGe-1 homozygous mutant larvae. Besides, overexpress dDcp2 in germline cells can complement microtubule disrupted phenotype in dGe-1 mutant GLC. This indicates that dGe-1 and dDcp2 have synergy effect on microtubule regulation. In addition, there is a physical interaction between dGe-1 C-terminal and dDcp2 C-terminal, which has been proven by yeast-two hybrid analysis in my study. Further, in immunofluorescence staining, dGe-1and dDcp2 are partial colocalized at oocyte cortex. In conclusion, we proposed that the physical interaction of dGe-1 and dDcp2 in oocyte is to regulate microtubule organization, which is responsible for oskar mRNA localizing and anchoring at posterior pole of oocyte | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:03:13Z (GMT). No. of bitstreams: 1 ntu-100-R98b43003-1.pdf: 10576906 bytes, checksum: c6bf24f8a44f3374a1d329744c08cf47 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | Table of contents
Introduction……………………………………………………………………...1 I. Processing body components…………………………………………..1 1. Processing body and mRNA degradation pathway………………………………...1 2. A dynamic movement of mRNA between P-bodies and the polysomes…………...2 3. Decapping protein 2, Dcp2, is the catalytic subunit of the decapping complex……3 4. Decapping protein 1, Dcp1, enhances Dcp2’s decapping activity in yeast………...4 5. dGe-1, the enhancer of decapping large subunit…………………………………...5 6. Other activators of decapping……………………………………………………....7 7. After decapping, mRNA is digested by Xrn1/Pacman which is 5’-3’ exoribonuclease…………………………………………………………………….8 II. Cell polarity determination and axial development in Drosophila oogenesis………………………………………………… ..9 1. Drosophila oogenesis……………………………………………………………9 2. Cell polarity determination relies on mRNA asymmetric localization…………10 3. The dorso-ventral axis determinant- gruken……………………………………10 4. The anterior determinant-bicoid………………………………………………..11 5. The posterior determinant- oskar……………………………………………….12 6. The general mechanism of mRNA asymmetric localization…………………...13 III. Microtubules organization and polarity in Drosophila oogenesis……………………………………………….................................14 1. Structures of microtubules………………………………………………………...14 2. Dynamic instability of microtubule……………………………………………….15 3. Microtubule regulator-microtubule-associated proteins (MAPs)…………………16 4. The biological function of microtubule in Drosophila development-microtubule dependent transport………………………………………………………………..18 5. The localization of oskar mRNA in the oocyte is depends on microtubule polarity…………………………………………………………………………….20 6. oskar mRNA transport and anchoring…………………………………………….21 7. Ooplasmic streaming ……………………………………………………………..22 8. Factors involved in streaming…………………………………………………….24 9. Biologic function of streaming……………………………………………………24 IV. Dynamic assembly of P-bodies and interaction with Cytoskeleton…………………………………………………………….…25 1. Assembly of P-bodies……………………………………………………………..25 2. P-bodies are associate with cytoskeleton………………………………………….27 3. P-body move on microtubules ……………………………………………………28 V. Previous studies on dGe-1……………………………………………..29 VI. The aim of this thesis…………………………………………………..31 Material and methods…………………………………………….….32 1. Fly handling…………………………………………….…………………………32 2. Fly stocks…………………………………………….……………………………32 3. Generation of transgenic flies……………………………………………………..33 4. Germ-line clones generation- combination of dominant female sterile( DFS) and FLP-FRT recombination technique……………………………………………….33 5. Ectopic expression by the GAL4-UAS system…………………………………...34 6. Immuno-fluorescent staining of Drosophila egg-chamber………………………..35 7. Immuno-fluorescent staining of microtubule in Drosophila ovary……………….36 8. Visualization of ooplasmic streaming……………………………………………..36 9. Western blotting of larvae lysate………………………………………………….36 10. RNA extraction……………………………………………………………………37 11. Reverse transcription PCR………………………………………………………..38 12. Yeast-two hybrid system………………………………………………………….38 | |
dc.language.iso | en | |
dc.title | 果蠅Processing body成員,dGe-1參與卵母細胞的細胞骨架組成 | zh_TW |
dc.title | Drosophila processing body component,dGe-1, is involved in cytoskeleton organization in the oocyte | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 柯逢春,王致恬,溫進德,黃偉邦 | |
dc.subject.keyword | decapping protein去頭蓋蛋白質,cytoskeleton細胞骨架,ooplasmic streaming細胞質流,dHedls促進去頭蓋大分子,oskar mRNA, | zh_TW |
dc.subject.keyword | decapping protein,cytoskeleton,ooplasmic streaming,dHedls,oskar mRNA, | en |
dc.relation.page | 149 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-08-15 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 10.33 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。