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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮 | |
dc.contributor.author | Han-Vei Jiang | en |
dc.contributor.author | 江漢威 | zh_TW |
dc.date.accessioned | 2021-06-08T07:02:22Z | - |
dc.date.copyright | 2009-02-10 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-02-03 | |
dc.identifier.citation | Abel, S., and Theologis, A. (1996). Early genes and auxin action. Plant Physiol 111, 9-17.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26187 | - |
dc.description.abstract | 植物多為固著生長,行光合作用,因此因應外界光線環境的改變,而調整自身的生長與發育能力,對植物尤其重要。在阿拉伯芥中,光敏素A 是負責接收遠紅光,並透過一連串的訊息傳遞而影響下游的基因表現,使植物進行光型態發生(photomorphogenesis)。前人研究指出GST 基因家族成員AtGSTU17在遠紅光中的基因表現量可受到phyA及其下游基因FIN219突變所抑制,隨著遠紅光強度越強,AtGSTU17也表現越多。在較弱的遠紅光下Atgstu17突變體呈現較野生型長的下胚
軸,顯示AtGSTU17的表現與遠紅光光照有密切的關係。但AtGSTU17在遠紅光中作用的分子機制尚未明瞭。利用遠紅光及PCR 篩選到Atgstu17phyA的雙突變體,從外表型的檢測,發現Atgstu17phyA的下胚軸長度在弱遠紅光下比親代各別的單突變體長。暗示AtGSTU17與phyA在遠紅光下有非等位非互補(nonallelic noncomplementation)的遺傳調控關係,可能分別調控同一訊息傳遞下的兩個平行途徑,或者兩者有相互作用的功能。另外,當AtGSTU17大量表現在phyA突變體(AtGSTU17OE-2/phyA),在遠紅光下的下胚軸長度比phyA突變體還短。然而,RT-PCR指出Atgstu17突變體在遠紅光下有幾個與auxin相關的基因表現增加,而PIFs及光訊息相關的基因(例如: CHS)的表現則是下降的。此外,在Atgstu17突變體有幾個受ABA調控的基因表現也受到影響。在鹽類逆境及滲透壓逆境處理之下,我們發現gstu17突變體對於逆境造成的側根抑制不敏感,這顯示 AtGSTU17可能有參與在逆境方面的調控。綜合上述結果顯示AtGSTU17的表現會受到遠紅光、荷爾蒙、逆境的調控,並且會影響植物在遠紅光下的光型態發生及荷爾蒙、逆境所控制的根部發育。 | zh_TW |
dc.description.abstract | Phytochrome A (phyA) is the photoreceptor of far-red (FR) light. Previous
studies have shown that a glutathione S-transferase (AtGSTU17), a tau class member of the GST gene family in Arabidopsis, can be induced rapidly in wild type under FR light, but abolished in the phyA mutant. The molecular mechanisms underlying the regulation of GSTU17 expression by phyA remain unknown. We isolated the gstu17phyA double mutant showing that it exhibited a longer hypocotyl phenotype than its parental lines specifically under weak FR light. When AtGSTU17 was overexpressed in the phyA mutant background (GSTU17OE-2/phyA), it led to a shorter hypocotyl phenotype than the phyA under FR light. These data suggest that AtGSTU17 and PHYA act as a nonallelic noncomplementary relation, and may work in parallel pathways or physically interact with each other to control hypocotyl elongation under FR. Furthermore, RT-PCR analyses indicated that several auxin responsive genes were up-regulated, and phytochrome-interacting factors (PIFs) as well as light responsive gene such as CHS down-regulated in the gstu17 mutant under FR. In addition, several ABA-regualted genes were affected in the gstu17 mutant. Under the ionic stress and osmotic stress treatments, the gstu17 mutant is hyposensitive in terms of lateral roots inhibition. Taken together, these data indicate that AtGSTU17 may function as a crosstalk among phyA-mediated FR light, auxin, ABA and stress signaling pathways to regulate hypocotyl elongation and root development in Arabidopsis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:02:22Z (GMT). No. of bitstreams: 1 ntu-98-R95b42013-1.pdf: 2936129 bytes, checksum: eea93df10c32c835fb5875a5c483a180 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 目錄 ............................................................................................................. I
中文摘要 .................................................................................................. IV 英文摘要 .................................................................................................... V 第一章 前言 ............................................................................................... 1 一、導讀 ............................................................................................................................... 1 二、植物的光訊息傳遞 ....................................................................................................... 1 1. Phytochromes 光敏素 ................................................................................................. 1 2.光訊息傳遞下游因子 ................................................................................................... 2 三、Auxin 與ABA 所影響的植物生長與基因表現 ............................................................ 3 四、活性氧分子(ROS) ......................................................................................................... 4 五、植物的抗氧化系統 ....................................................................................................... 5 六、植物GSTs 的歷史及分類 ............................................................................................ 6 七、植物GSTs 的功能 ......................................................................................................... 7 八、GST 與光的研究 ............................................................................................................ 7 九、AtGSTU17 先前的研究 .................................................................................................. 8 十、逆境與GSH 系統 ........................................................................................................... 8 十一、研究目標 ................................................................................................................... 9 第二章 材料與方法 ................................................................................ 10 2-1 植物材料與生長條件 .................................................................................................. 10 2-2 Genomic DNA 萃取 ...................................................................................................... 10 2-3 分析T-DNA homozygous lines ................................................................................ 11 2-4 RNA 的萃取與RNA 表現量分析 .................................................................................. 11 2-5 花青素與葉綠素以及開花時間的測量 ...................................................................... 12 II 2-6 根毛觀察 ...................................................................................................................... 12 2-7 種子發芽測試 .............................................................................................................. 12 2-8 GUS 活性染色分析 ...................................................................................................... 13 2-9 Glutathione 萃取 ...................................................................................................... 13 2-10 逆境耐受程度測試 ................................................................................................... 14 第三章 結果............................................................................................. 15 一、利用遠紅光篩選及PCR 方法獲得Atgstu17phyA 雙突變株 ................................... 15 二、Atgstu17phyA 雙突變株在弱遠紅光下,其下胚軸長度比phyA 長 ...................... 15 三、GSTU17OE-2/phyA 在遠紅光下,其下胚軸長度較phyA 短 .................................... 16 四、AtGSTU17 影響在遠紅光下花青素累積、葉綠素生合成以及開花時間 ............... 16 五、AtGSTU17 在遠紅光下會影響光訊息及auxin 相關的基因表現 ............................ 17 六、Atgstu17 的側根與根毛發育與野生型不同 ............................................................ 18 七、AtgstU17、phyA、AtgstU17phyA 對於ABA 抑制根生長不敏感 ............................ 18 八、phyA 對於ABA 所影響的種子發芽及發育不敏感 .................................................... 18 九、AtGSTU17 會影響ABA 及與逆境相關的基因表現 .................................................... 19 十、Atgstu17 對於鹽類逆境或者滲透壓逆境不敏感 .................................................... 19 十一、pAtGSTU17:GUS 轉殖株經Auxin 及ABA 及滲透壓逆境處理之後,AtGSTU17 表現 在根尖及側根生長點 ......................................................................................................... 20 十二、AtGSTU17 影響植物內生的GSH level ................................................................. 21 第四章、討論........................................................................................... 22 AtGSTU17 參與在遠紅光訊息傳遞之中 ............................................................................ 22 AtGSTU17 影響Auxin 及ABA 所調控的植物生理 ............................................................ 24 AtGSTU17 影響ABA 及與逆境相關的基因表現 ................................................................ 25 AtGSTU17 參與鹽類及滲透壓逆境的調控 ........................................................................ 26 AtGSTU17 對於植物體內GSH 含量恆定是重要的 ............................................................ 26 結論 .................................................................................................................................... 27 III 參考文獻 ................................................................................................... 46 附錄 ........................................................................................................... 56 一、AtGSTU17 與phyA 在阿拉伯芥第一條染色體上的位置 .......................................... 56 二、fin219 與phyA 雜交所得F1 世代在遠紅光下的外表型 ........................................ 57 三、AtGSTU17 T-DNA 插入位置示意圖 ............................................................................ 58 四、本文中所使用的phyA 突變株 ................................................................................... 59 五、microarray data ...................................................................................................... 60 | |
dc.language.iso | zh-TW | |
dc.title | 阿拉伯芥AtGSTU17與PHYA在光、荷爾蒙及逆境的功能研究 | zh_TW |
dc.title | Functional Studies of Glutathione S-Transferase
(AtGSTU17)and Phytochrome A in Response to Light,Hormones and Stress Conditions | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王淑珍,林讚標,鄭秋萍 | |
dc.subject.keyword | 阿拉伯芥,光敏素,離層素,遠紅光, | zh_TW |
dc.subject.keyword | Arabidopsis,phytochrome,ABA,Far-red,GST, | en |
dc.relation.page | 62 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2009-02-03 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 2.87 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。